Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) from MedChemExpress

Product Specs
ItemProtease Inhibitor Cocktail, mini-Tablet (EDTA-Free)
CompanyMedChemExpress
Price $120.00Supplier Page View Company Product Page
Catalog NumberHY-K0011
Quantity25 pcs
ApplicationsBioactivity, Structure and function analysis, ELISA, SDS-PAGE, Western blotting
Typekit

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(1) Dihydromyricetin confers protection against myocardial ischemia-reperfusion injury by inhibiting ferroptosis through direct targeting of PPARαFront PharmacolApril 10, 2026Binwei Jin, Yang Zhang, Jiangbo Lin, Yu Zhu, Mengqi Yang, Yutao Zhang, Yanping Yin, Zhiming Zhang, Minjun Yang, Jianjun Jiang, et al.H9c2 cells and heart tissues using RIPA lysis buffer (Beyotime, China) containing protease inhibitors (HY-K0011; MedChemExpress, United States). Protein concentration was determined by the BCA method (P0010S; Beyotime

(2) A spontaneous termination mechanism of RNA polymerase V shapes the DNA methylation landscape in plantsThe EMBO JournalApril 2, 2026Guohui Xie, Xuan Du, Yifang Tan, Yuxing Zhou, Cheng Chi, Sixian Zhou, Colette L Picard, Songge Chai, Lei Wu, Danling Zhu, et al.MgCl2, 2 mM DTT, 5 mM CaCl2, 25% glycerol, 0.2 mM PMSF, 1× protease inhibitor cocktail (MedChemExpress, HY-K0011), and 50 mM Tris-HCl, pH 7.5. The nucleus was disrupted by osmotic shock. Micrococcal Nuclease (MNase,

(3) Short-chain acyl-CoA dehydrogenase initiates mtDNA demethylation and leakage to fuel antitumor immunity in colorectal cancerSignal Transduct Target TherMarch 26, 2026Fang Yang, Meng Wang, Shaofan Hu, Xu Guan, Kun Zhao, Yong Zhou, Hui Yao, Tianying Zhang, Liuli Li, Yuan Gao, et al.in cell lysis/IP buffer (#P0013, Beyotime, Shanghai, China) supplemented with protease inhibitors (#HY-K0011, MedChemExpress, Shanghai, China). The protein concentration of the lysates was detected via an enhanced

(4) METTL16-mediated m6A modification of MSMO1 modulates cholesterol metabolism and activates MAPK-p38/NF-κB signaling in colorectal cancerJournal of Experimental & Clinical Cancer ResearchMarch 17, 2026Yongheng Zhao, Tingyue Gong, Hao Li, Haiping Lin, Minhao Yu, Yang Luo, Ming Zhong, Jun Qinwere lysed in RIPA buffer (#P0013K, Beyotime, China) supplemented with a protease inhibitor cocktail (#HY-K0011, MCE, USA) and a phosphatase inhibitor cocktail III (#HY-K0023, MCE, USA) to extract total cellular

(5) Neuropilin-2 Deficiency Promotes Mitochondrial Dysfunction and NAD⁺-Dependent Cellular Senescence in Retinal DegenerationInvest Ophthalmol Vis SciMarch 5, 2026Lechun Ou, Baoyue Cui, Linbin Zhou, Keli Liu, Le Shi, Tian Zhou, Lei Zeng, Hong Zhou, Huiyi Xu, Yingfeng Zheng, et al.clustering. Western Blotting and RT-qPCR Cells were lysed in RIPA buffer with protease/phosphatase inhibitors (HY-K0011/HY-K0023; MedChemExpress). Proteins were separated by SDS-PAGE, transferred to PVDF membranes, and probed

(6) Targeting leucine-rich repeat kinase 2 overcomes resistance to oncolytic herpes simplex virus-based therapies in glioblastomaNature CommunicationsFebruary 28, 2026Yaning Qin, Zhiqi Liang, Mengqin Yu, Nanqian Lin, Naizhen Wang, Xuanke Huang, Shi Chen, Qun Peng, Yanhua Lin, Heqin Wang, et al.Phosphatase inhibitor cocktail III (TopScience, C0004), Protease inhibitor cocktail (MedChemExpress, HY-K0011), and RIPA lysis buffer (Beyotime, P0013C). Crystal violet (BBI, CB0331), Recombinant Human CD300a Protein

(7) Endothelial UNC5B regulates blood‑retinal barrier homeostasisInternational Journal of Molecular MedicineFebruary 20, 2026Yujia Yao, Suyu Wang, Jiajun Li, Qianzi Jin, Ziyi Chen, Qin Jiang, Keran Libuffer (cat. no. P0013B; Beyotime Biotechnology) supplemented with protease inhibitor cocktail (cat. no. HY-K0011; MedChemExpress). Protein concentrations were determined using the BCA assay (cat. no. 23225; Thermo Fisher Scientific

(8) Cerebellar microglia-derived IL-17A mitigates autism-related behavioral and synaptic deficitsMolecular PsychiatryFebruary 9, 2026Jun Yin, Wei Li, Li-Ping Shen, Wen-Lei Zhang, Jun-Yi Chen, Bei-Bei Zhang, Yi-Jie Chen, Tong Li, Hong-Zhao Li, Zhenyu Gao, et al.homogenized using an electric homogenizer in ice-cold PBS containing protease inhibitors (MedChemExpress, HY-K0011). The homogenates were then centrifuged at 1500 g for 10 min to remove pellet debris, and the supernatant

(9) Dual genomic localizations and gene regulatory functions of MBD-2 with and without NuRD in Caenorhabditis elegans which lacks DNA methylationNature CommunicationsJanuary 20, 2026Hok Ning Tsui, Charmaine Yan Yu Wong, Chaogu Zheng, Karen Wing Yee Yuensolubilized by adding EDTA to a final 10 mM concentration, one protease inhibitor tablet (MCE Cat# HY-K0011) and Triton X-100 to a final 1% concentration, nutated at 4 °C for 2 hr. Two volumes of cold 150 mM

(10) A CD25–chemokine receptor complex initiates noncanonical IL-2 signalingJournal of Biological ChemistryJanuary 1, 2026Ho-Sup Lee, Sarah Hyun Ji Kim, Javid Aceil, Amelia Meecham, Alexandre Gingras, Klaus Ley, Jamie B. Spangler, Mark H. GinsbergmM CaCl2, 0.5 mM MgCl2, and an EDTA-free protease inhibitor cocktail (MedChemExpress; catalog no.: HY-K0011). Lysates were then centrifuged at 12,000 rpm for 15 min to remove insoluble debris. The resulting lysates

(11) DHCR7 Promotes Liver Metastasis of Pancreatic Cancer Through PI3K ‐Akt Signaling PathwayCancer ScienceNovember 1, 2025Yu Xie, Ziyue Zhou, Jiangwei Song, Chengzhi Wang, Yibing Ding, Yudong Qiu, Lei Fang, Hao ChengWestern Blot Analysis Following ice‐cold RIPA buffer lysis (AR0105, Beyotime, China) which contains Protease Inhibitor Cocktail (HY‐K0011, MCE, USA), PMSF (HY‐B0496, MCE, USA) and Phosphatase Inhibitor Cocktail I (HY‐K0021, MCE, USA), cells

(12) Protocol to investigate the biochemical details of immune checkpoint ligand/receptor ubiquitination using in vitro ubiquitination assaySTAR ProtocOctober 17, 2025Guojiao Xie, Lin Gao, Linxia Tian, Xinning Li, Tiantian Zheng, Xian Yu, Hanjie Jiang, Zan ChenBiotech,Cat#E607008 X-Gal solution,Sangon Biotech,Cat#B541006 Protease inhibitor cocktail (EDTA-free),MedChemExpress,Cat#HY-K0011 Magnesium chloride anhydrous (MgCl2),Adamas-beta,Cat#829999A TM buffer,Beyotime,Cat#ST455 LipoInsect,Beyotime,Cat#C0551 Bovine

(13) DCAF7 recruits USP2 to facilitate hepatocellular carcinoma progression by suppressing clockophagy-induced ferroptosisCell Death DisAugust 28, 2025Honglv Jiang, Xiaohui Wang, Zhenhua Zhu, Cheng Song, Dan Li, Yixuan Yun, Li Hui, Leilei Bao, Darran P. O’Connor, Jingjing Ma, et al.HY-17589A, RRID: SCR_023066), MLN4924 (HY-70062), and protease inhibitor cocktail mini-tablet (EDTA-free, HY-K0011, RRID: SCR_022150) were purchased from MedChemExpress (MCE). CoCl2 (409332) was ordered from Sigma–Aldrich. Cell

(14) NSUN2 promotes colorectal cancer progression by stabilizing PHGDH mRNA to promote serine metabolism reprogrammingCancer MetabAugust 14, 2025Hao Li, Tingyue Gong, Yongheng Zhao, Yang Luo, Shuibin Tang, Tingfeng Wang, Haiping Lin, Ming Zhonglysed in RIPA lysis buffer (#20114ES, YEASEN, China) supplemented with protease inhibitor cocktail (#HY-K0011, MedChemExpress, USA). Western blotting analysis was performed following standard protocols, and target

(15) O-GalNAc Glycosylation Activates MBL-Mediated Complement and Coagulation Cascades to Drive Organotropic MetastasisAdvanced science (Weinheim, Baden-Wurttemberg, Germany)August 1, 2025Chen X, Bao W, Liu K, Jing N, Du G, Jiang L, You Q, Zhang Y, Xu P, Cheng C, et al.using the RIPA lysis buffer (Thermo Fisher) supplemented with Protease Inhibitor Cocktail (PIC, MCE, HY‐K0011). Proteins of interest were separated by SDS‐PAGE and electrophoresis, and transferred onto the polyvinylidene

(16) ACE-tRNAs are a platform technology for suppressing nonsense mutations that cause cystic fibrosisNucleic Acids ResJuly 12, 2025Wooree Ko, Joseph J Porter, Sacha Spelier, Emily G Sorensen, Priyanka Bhatt, Jeffrey T Gabell, Isabelle van der Windt, Tyler Couch, Kevin Coote, Martin Mense, et al.RIPA buffer (Thermo Scientific, Cat. # 89900) containing 1× protease inhibitors (MedChemExpress, Cat. # HY-K0011) was added to each well. The cells were mechanically dislodged from the bottom of the well, and the

(17) Protocol for high-throughput screening of SIRT7 inhibitors using fluorescent peptide technologySTAR ProtocolsJune 1, 2025Tian-Shu Kang, Xiaopeng Lu, Jinke Gu, Yuan Tian, Wei-Guo Zhu(DMSO),Solarbio,Cat#D8371 Dithiothreitol (DTT),Beyotime,Cat#ST041 PMSF,MCE,Cat#HY-B0496 Protease inhibitor cocktail,MCE,Cat#HY-K0011 Sodium chloride (NaCl),Macklin,Cat#S805281 Magnesium chloride (MgCl2),Millipore,Cat#814733 Tris,Solarbio,Cat#T8060 95%

(18) Effect modification by socioeconomic status on the associations between early placental protein damage and exposure to ambient air PM 2.5 chemical componentsEnvironmental Health PerspectivesMay 23, 2025Junnan Yang, Xuesong Li, Mengyu Chu, Mengyuan Wang, Yan Huo, Bin Han, Zhipeng Bai, Jianmei Wang, Yujuan Zhangmixed with eight times its mass of phosphate buffer solution and moderate protease inhibitor (number HY-K0011; MedChemExpress) and homogenized at 6.5m/s for five cycles at 4°C. After centrifugation of the villi

(19) Effect Modification by Socioeconomic Status on the Associations between Early Placental Protein Damage and Exposure to Ambient Air PM2.5 Chemical ComponentsEnviron Health PerspectMay 23, 2025Junnan Yang, Xuesong Li, Mengyu Chu, Mengyuan Wang, Yan Huo, Bin Han, Zhipeng Bai, Jianmei Wang, Yujuan Zhangmixed with eight times its mass of phosphate buffer solution and moderate protease inhibitor (number HY-K0011; MedChemExpress) and homogenized at 6.5m/s for five cycles at 4°C. After centrifugation of the villi

(20) EIDD-2801 resists to infection and co-infection of SARS-CoV-2 and influenza virusVirol JApril 28, 2025Bingshuo Qian, Rongbo Luo, Beilei Shen, Lingjun Fan, Junkui Zhang, Shijun Zhang, Yan Sun, Xiuwen Deng, Xiaobin Pang, Wu Zhong, et al.Radio Immunoprecipitation Assay Lysis (RIPA) buffer (Beyotime, China) with protease inhibitor (MCE, HY-K0011, Shanghai, China) for Western Blot (WB) or in DMEM for quantification of the viral titer. Co-infection

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Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) from MedChemExpress

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Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) from MedChemExpress

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