IPTG from MedChemExpress

Product Specs
ItemIPTG
CompanyMedChemExpress
Price $61.00Supplier Page View Company Product Page
Catalog NumberHY-15921
Quantity10 mM * 1 mL
ApplicationsBioactivity, Structure and function analysis, ELISA, SDS-PAGE, Western blotting
Purity99.93%
TypeChemical
Molecular Weight238.3
FormulaC₉H₁₈O₅S
Research AreaOthers

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(1) Linking sugar sensing to immunity in plants through O-glycosylation of immune-signaling kinasesbioRxivJuly 8, 2026Yalikunjiang Aizezi, Hongliang Zhang, Hongliang Zhang, Jung-Gun Kim, Ajeet Chaudhary, Cao Son Trinh, Qingxi J. Shen, Shou-Ling Xu, Mary Beth Mudgett, Zhi-Yong WangScientific,A32957 Protease Inhibitor Cocktail,Thermo Scientific,3214605 PMSF,Sigma-Aldrich,P7626 IPTG,MedChemExpress,HY-15921 DTT,Goldbio,DTT10 Immidazole,Sigma-Aldrich,I-2399 L-Glutathione reduced,Sigma-Aldrich,G6629 Luminol,Sigma-Aldrich,A8511 Peroxidase from horseradish

(2) Protocol to study membrane-associated protein phase separation using protein-lipid blot overlay assays and giant unilamellar vesicles in vitroSTAR ProtocMay 14, 2026Chenyi Xie, Wei Wei, Lujia You, Xiaofang Cui, Ze-Guang HanBiotech,Cat#B548138 PEG8000,BBI,Cat#A600433 Tris Base,BBI,Cat#A501492 Sucrose,BBI,Cat#A420074 NaCl,BBI,Cat#A610477 IPTG,MCE,Cat#HY-15921 Imidazole,Sigma-Aldrich,Cat#206-019-2 Tween-20,BBI,Cat#A631023 PMSF,BBI,Cat#A430281 Glycerol,BBI,Cat#A600232 LB

(3) Renal coenzyme A (CoA) production from VB5 fuels stem cell proliferation and tumor growthNature CommunicationsApril 18, 2026Ting Miao, Ying Liu, Mujeeb Qadiri, Amaury Dasseux, John M. Asara, Yanhui Hu, Xiaomei Sun, Luz del Carmen Pliego-Alcántara, Christian C. Dibble, Norbert Perrimonprotein expression was induced with 250 µM isopropyl β-D-1-thiogalactopyranoside (IPTG; MedChemExpress, HY-15921) for 3 hrs. Bacteria were pelleted by centrifugation, washed, and resuspended with PBS at 4 °C, pelleted

(4) YEATS2 promotes DNA repair and induces anoikis resistance by enhancing chromatin accessibility to drive prostate cancer metastasisOncogeneFebruary 18, 2026Haoran Li, Yarong Song, Yukun Cong, Chuxiong Wang, Kang Chen, Chunyu Liu, Menghao Zhou, Yunjie Ju, Jinyu Chen, Liang Chen, et al.(PAIVIBIO, China). The pGEX-6P-1 vector was induced with 1 mM Isopropyl β-D-thiogalactoside (IPTG) (HY15921, MCE) for 12 h, and the GST-YEATS domain was purified following the protocol of the GST-tag Protein

(5) HSPA5 promotes YAP/TAZ stability independently of the Hippo pathway and induces proneural-to-mesenchymal transition in glioblastomaCell Death DisFebruary 7, 2026Shikai Gui, Wanli Yu, Zhen Song, Lunshan Peng, Haitao Luo, Kai Huang, Juexian Xiao, Jiabao Xie, Shihao Cai, Shengtao Yuan, et al.and GST-TAZ plasmids (OBIO Technology Company, Shanghai, China) were transfected into BL21 E. coli (#HY-15921, MCE, USA), and the culture was shaken overnight at 17 °C for protein amplification. The fusion protein

(6) Ginsenoside Rg5 Targets PRDX1 to Disrupt Redox Homeostasis and Induce Mitochondria-Dependent Apoptosis in Human Hepatocellular Carcinoma HepG2 CellsMoleculesFebruary 5, 2026Hai-Lun Ye, Ya-Ni Wang, Gang-Ao Li, Xing-Hui Jin, Guan-Ting Wu, Yang Li, Ying-Hua Jininduced by adding 0.2 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; MCE, Monmouth Junction, NJ, USA, HY-15921), followed by incubation at 16 °C for 16–18 h. After induction, the bacterial cells were harvested by

(7) Manganese activates the CBASS immunity to protect bacteria from phage infectionmBioJanuary 14, 2026Xiao Wang, Yongdong Li, Xiao Wang, Wenjing Zhang, Muohua Liu, Xinwei Hao, Shukun Chen, Tianyuan Chang, Conghui Wu, Chonghua Hao, et al.ΔmntH, ΔmntR, and ΔmntP mutants were successively eliminated by β-D-1-thiogalactopyranoside (IPTG) (Cat# HY-15921, CAS: 367-93-1, MCE) induction and overnight incubation at 37°C, respectively. Determination of metal

(8) Extracellular vesicle-based targeted protein degradation platform for multiple extracellular proteinsEMBO Molecular MedicineJanuary 12, 2026Bide Tong, Xiaoguang Zhang, Dingchao Zhu, Yulei Wang, Junyu Wei, Zixuan Ou, Huaizhen Liang, Hanpeng Xu, Zhengdong Zhang, Jie Lei, et al.Kit,Invent,LY-034 NHS-sulfo-biotin,APEXBIO,A8001 Protein A/G Magnetic Beads,MedChemExpress,HY-K0202 IPTG,MedChemExpress,HY-15921 GST-pull down Kit,Beyotime,P2262 Proximity Ligation Assay Kit,Naveni,60025 Lipo2000,Thermo Fisher,11668030 TRIzol

(9) Engineered outer membrane vesicles enhance solid tumour CAR-T cell therapyNature Biomedical EngineeringJanuary 7, 2026Xianjun Li, Xuehan Li, Jiaqi Shi, Yingjing Li, Hanyu Zhang, Tianjun Chen, Hui Pang, Shuyuan Zhang, Shengnan Luo, Fengyi Liu, et al.0.6–0.8. PD-L1 expression was induced with 1:1,000 1 M isopropyl β-D-1-thiogalactopyranoside (CAS no. HY-15921, MedChemExpress) diluted 1:1,000 in lysogeny broth broth. Incubated overnight at 18 °C, the bacteria

(10) LGR5 interacts with HSP90AB1 to mediate enzalutamide resistance by activating the WNT/β-catenin/AR axis in prostate cancerChinese Medical JournalDecember 5, 2025Ze Gao, Zhi Xiong, Yiran Tao, Qiong Wang, Kaixuan Guo, Kewei Xu, Hai Huangtag. The GST-LGR5 plasmid was transfected into Escherichia coli. Isopropylthio-β-galactoside (IPTG) (#HY-15921, MCE, NJ, USA, 200 ng/mL) was added, and the culture was shaken at 17°C overnight for protein amplification.

(11) RNA m1A methyltransferase TRMT61A promotes colorectal tumorigenesis by enhancing ONECUT2 mRNA stability and is a potential therapeutic targetCancer CommunicationsOctober 16, 2025Xiaoting Zhang, Na Qin, Fenfen Ji, Hao Su, Haiyun Shang, Hongyan Chen, Dan Huang, Qing Li, Jing Ren, Weixin Liu, et al.until reaching an OD600 of 0.6–0.8. Protein expression was induced with 0.5 mmol/L IPTG (1 mmol/L, # HY‐15921, MedChemExpress) at 18°C overnight with shaking. Cells were harvested and lysed by sonication on ice.

(12) Cancer-associated fibroblasts regulate mitochondrial metabolism and inhibit chemosensitivity via ANGPTL4-IQGAP1 axis in prostate cancerJournal of Advanced ResearchSeptember 1, 2025Zhi Xiong, Rui-Lin Zhuang, Shun-Li Yu, Zhao-Xiang Xie, Shi-Rong Peng, Ze-An Li, Bing-Heng Li, Jun-Jia Xie, Yi-Ning Li, Kai-Wen Li, et al.10 h to amplify the protein. Afterward, 0.5mM isopropyl β-D-thiogalactoside (IPTG) (MedChemExpress, HY-15921) was added to the bacterial solution, which was then shaken and cultured for 6h at 37 °C. The bacteria

(13) LGR5 interacts with HSP90AB1 to mediate enzalutamide resistance by activating the WNT/β-catenin/AR axis in prostate cancerChin Med J (Engl)April 25, 2025Ze Gao, Zhi Xiong, Yiran Tao, Qiong Wang, Kaixuan Guo, Kewei Xu, Hai Huangtag. The GST-LGR5 plasmid was transfected into Escherichia coli. Isopropylthio-β-galactoside (IPTG) (#HY-15921, MCE, NJ, USA, 200 ng/mL) was added, and the culture was shaken at 17°C overnight for protein amplification.

(14) Macrophage SUCLA2 coupled glutaminolysis manipulates obesity through AMPKNat CommunFebruary 18, 2025Chang Peng, Haowen Jiang, Liya Jing, Wenhua Yang, Xiaotong Guan, Hanlin Wang, Sike Yu, Yutang Cao, Min Wang, Huan Ma, et al.and rotated at a 37 °C, 250 rpm until the OD595 of the medium reached 0.4 to 0.6. Next, 100 μM IPTG (#HY-15921, MCE) was added to the LB medium to induce protein expression at 16 °C, 180 rpm for 16 h. Then, the

(15) Gut-liver translocation of pathogen Klebsiella pneumoniae promotes hepatocellular carcinoma in miceNat MicrobiolJanuary 2, 2025Xueliang Wang, Yi Fang, Wei Liang, Yuhong Cai, Chi Chun Wong, Junlin Wang, Na Wang, Harry Cheuk-Hay Lau, Ying Jiao, Xingyu Zhou, et al.grown in ampicillin-supplemented LB broth at 37 °C. When optical density reached 1.0, 0.5 mM IPTG (HY-15921, MCE) was added to induce protein synthesis at 20 °C overnight. Bacterial pellets were collected and

(16) m 6 A Modification of Profilin-1 in Vascular Smooth Muscle Cells Drives Phenotype Switching and Neointimal Hyperplasia via Activation of the p-ANXA2/STAT3 PathwayArteriosclerosis, Thrombosis, and Vascular BiologyDecember 1, 2024Xiao-Fei Gao, Ai-Qun Chen, Hao-Yue Tang, Xiang-Quan Kong, Huan Zhang, Zhi-Mei Wang, Wei Lu, Li-Guo Wang, Feng Wang, Wen-Ying Zhou, et al.GST-PFN1 protein. The GST protein was purified using IPTG (isopropyl-beta-D-thiogalactopyranoside; HY-15921, MCE), and the bound ANXA2 was detected through Western blot analysis. Statistics The results are

(17) m6A Modification of Profilin-1 in Vascular Smooth Muscle Cells Drives Phenotype Switching and Neointimal Hyperplasia via Activation of the p-ANXA2/STAT3 PathwayArterioscler Thromb Vasc BiolNovember 7, 2024Xiao-Fei Gao, Ai-Qun Chen, Hao-Yue Tang, Xiang-Quan Kong, Huan Zhang, Zhi-Mei Wang, Wei Lu, Li-Guo Wang, Feng Wang, Wen-Ying Zhou, et al.GST-PFN1 protein. The GST protein was purified using IPTG (isopropyl-beta-D-thiogalactopyranoside; HY-15921, MCE), and the bound ANXA2 was detected through Western blot analysis. Statistics The results are

(18) Tripartite motif containing 26 prevents steatohepatitis progression by suppressing C/EBPδ signalling activationNature CommunicationsOctober 11, 2023Minxuan Xu, Jun Tan, Xin Liu, Li Han, Chenxu Ge, Yujie Zhang, Fufang Luo, Zhongqin Wang, Xiaoqin Xue, Liangyin Xiong, et al.stimulated by treatment with 0.5 mM isopropyl β-D-thiogalactoside (IPTG) obtained from MedChemExpress (Cat: HY-15921) located in Shanghai, China. Next, the extractions were subjected to co-treatment with the respective

(19) USP13 deubiquitinates and stabilizes cyclin D1 to promote gastric cancer cell cycle progression and cell proliferationOncogeneJuly 1, 2023Ma C, Wang D, Tian Z, Gao W, Zang Y, Qian L, Xu X, Jia J, Liu Z.Escherichia coli BL21 (DE3) cells, which were induced by isopropyl-b-D-thiogalactoside (IPTG, MedChemExpress, HY-15921). Cyclin D1 expression vector was transfected into HEK293T cells. The GST tagged USP13 protein was purified

(20) The deubiquitinating enzyme 13 retards non-alcoholic steatohepatitis via blocking inactive rhomboid protein 2-dependent pathwayActa Pharmaceutica Sinica BMarch 1, 2023Minxuan Xu, Jun Tan, Liancai Zhu, Chenxu Ge, Wei Dong, Xianling Dai, Qin Kuang, Shaoyu Zhong, Lili Lai, Chao Yi, et al.competent cell system, then followed by activated with isopropyl β-d-thiogalactoside (IPTG) (Cat.: HY-15921, MedChemExpress, Shanghai, China). 2.12 In vivo and in vitro binding ubiquitination detection The

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