Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) from MedChemExpress

Product Specs
ItemProtease Inhibitor Cocktail (EDTA-Free, 100× in DMSO)
CompanyMedChemExpress
Price $35.00Supplier Page View Company Product Page
Catalog NumberHY-K0010
Quantity1 mL
ApplicationsBioactivity, Structure and function analysis, ELISA, SDS-PAGE, Western blotting
Typekit

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(1) MLPH/RAB3A accelerates the differentiation of pancreatic stem cells to islet β-cells to control blood glucose in diabetic ratsOrganogenesisDecember 31, 2026Junling Shan, Huifeng Wang, Guangyu ZhuShanghai, China; Cat. No. P0013J) containing protease inhibitor cocktail (MedChemExpress, USA; Cat. No. HY-K0010), followed by centrifugation at 12,000 g for 30 min and supernatant removal. An aliquot containing 30

(2) Streptococcus suis Stk1 sensitizes epithelial cells to ferroptosis and exacerbates disruption of the respiratory epithelial barrierEmerging Microbes & InfectionsDecember 31, 2026Lang Tian, Ruicheng Yang, Ting Qi, Wenquan Ouyang, Hongshuo Liu, Dong Huo, Hang Li, Chuyue Zhou, Manman Xu, Haojie Li, et al.buffer (MedChemExpress, HY-K1001) supplemented with a protease inhibitor cocktail (MedChemExpress, HY-K0010). Protein concentrations were determined using a BCA protein assay kit. Equal amounts of protein were

(3) Equine infectious anemia virus blocks interferon responses through Rev-mediated activation of the stress granule-PKR-eIF2α pathwayPLoS PathogMay 21, 2026Huiling Ren, Kewei Chen, Bingqian Zhou, Weiguo Zhang, Xue-Feng Wang, Xiaojun Wang[Sigma-Aldrich, T7878], 5 mM EDTA [Sigma-Aldrich, E6758]) containing 1% Protease Inhibitor Cocktail (MCE, HY-K0010). Proteins were separated by 4–20% SDS-PAGE and transferred to nitrocellulose membranes. After blocking

(4) UBE2M-mediated EGFR neddylation drives keratinocyte proliferation in psoriasisiScienceMay 15, 2026Xiang Lin, Qixia Wang, Linlin Xu, Yibo Wang, Yixuan Fu, Zhenzhen Yan, Shuhong Huang, Ningning Dangbuffer (CWBIO, Cat# CW2333S) supplemented with a protease inhibitor cocktail (MedChemExpress, Cat# HY-K0010) and a phosphatase inhibitor cocktail (CWBIO, Cat# CW2383S). Protein concentrations were determined

(5) Infectious bursal disease virus suppresses humoral immunity via blocking antibody affinity maturation in chickensiScienceMay 15, 2026Yuxin Guo, Zhaocan Zhou, Huixuan Jiao, Li Gao, Xiaoqi Li, Hong Cao, Yongqiang Wang, Shijun J. Zhengafter transfection and lysed in lysis buffer supplemented with protease inhibitor cocktail (MCE, Cat#HY-K0010). Proteins were separated by SDS-PAGE and transferred to PVDF membranes. Membranes were incubated with

(6) TRIM25 promotes antiviral innate immune response by stabilizing IRF7 and its nuclear translocationmBioMay 13, 2026Zhen Wu, Yingxuan Cui, Yu He, Tao Wang, Tao Hu, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Xinxin Zhao, et al.Lysis Buffer (Thermo Fisher Scientific, 87787) supplemented with protease inhibitor cocktail (MCE, HY-K0010). Lysates were clarified by centrifugation at 12,000 × g for 10 min at 4°C. For immunoprecipitation,

(7) Integrative Multi-Omics Reveal Silibinin Alleviates Heat Stress-Driven Hepatic Lipid Disruption in Laying HensInt J Mol SciMay 11, 2026Jiang Gao, Hongrui Ren, Xuanfu Wu, Cunzhi Zou, Bin He, Wenqiang Maice-cold RIPA buffer (P0013B, Beyotime, Shanghai, China), supplemented with protease inhibitor cocktails (HY-K0010, MCE, South Brunswick Township, New Jersey, USA). Protein concentrations were determined by BCA assay

(8) Talin1 loss activates DRG neurons to accelerate bone remodeling and fracture healing in miceJ Orthop TranslatMay 8, 2026Qinnan Yan, Donghao Gan, Kangtai Xu, Zecai Chen, Sixiong Lin, Bo Zhou, Zhen Xu, Qiyue Zhong, Chu Tao, Yiming Zhong, et al.lysed using RIPA buffer (Sigma–Aldrich; R0278) supplemented with 1% Protease Inhibitor Cocktail (MCE; HY-K0010). Then, the cellular proteins were extracted and sequentially underwent electrophoresis, membrane transfer,

(9) Elevated TRIM25 Impairs Poly (ADP‐ribose) Metabolism via PARG Degradation and Mediates Compression‐Induced Intervertebral Disc DegenerationAdvanced ScienceMay 1, 2026Zhangrong Cheng, Haiyang Gao, Wenbo Wu, Pengzhi Shi, Xianglong Chen, Zimu Yu, Wang Wu, Kangcheng Zhao, Cao Yang, Yukun Zhang(Cat# P0013B, Beyotime, China,) supplemented with 1:100 dilution of protease inhibitor cocktail (Cat# HY‐K0010, MedChemExpress, China). The lysates were incubated on ice for 30 min and subsequently sonicated to

(10) Lumican-TLR4 interaction accelerates hepatic fibrosis by activating hepatic stellate cellsHepatol CommunMay 1, 2026Yongqiang Xiong, Huanhuan Zhang, Ao Wang, Yaqian Cui, Jiaxi Ye, Leiming Jin, Mengsha Lin, Qianhui Zhang, Guang Liang, Wu Luo, et al.http://links.lww.com/HC9/C327. Western blot analysis RIPA buffer (Cat: P0013B, Beyotime) containing a protein inhibitor cocktail (Cat: HY-K0010; MCE, NJ, USA) was used to prepare liver tissue and cell lysates. The total protein extract was separated

(11) A sterol-binding pocket in iRhom1 underlies paralog-specific regulation of the sheddase ADAM17bioRxivMay 1, 2026Fangfang Lu, Hongtu Zhao, Yaxin Dai, Chia-Hsueh Lee, Matthew Freeman12338018 Sodium butyrate,Millipore-Sigma,Cat# 303410 Protease Inhibitor Cocktail,Medchemexpress,Cat# HY-K0010 Benzonase nuclease,Millipore-Sigma,Cat# E1014-25KU n-Dodecyl-β-D-Maltopyranoside (DDM),Anatrace,Cat#

(12) Homocysteine Drives Hippocampal Blood–Brain Barrier Disruption and Cognitive Decline Under Chronic Stress via DNA Hypomethylation of Cav1.2Brain SciencesApril 30, 2026Mao-Yang Zhou, Jin-Shan Li, Zhao-Xin Sun, Jie Yin, Yun Zhao, Fang Xie, Xue Wang, Sheng-Hui Zhang, Zhao-Wei Sun, Ling-Jia Qian(Applygen, Dhaka, Bangladesh, C1053+) supplemented with protease inhibitor cocktail (Mce, Singapore, HY-K0010) at 1 mL per 100 mg of tissue, and homogenized using an ultrasonic homogenizer. After lysis on a shaker

(13) Targeting adipocyte ESRRA alleviates osteoarthritis via interrupting inter-organelle crosstalk of complement C3-CFD-MAC cascadeBone ResApril 29, 2026Tongling Huang, Zihui Wang, Lu Gao, Jun Gao, Zhaocheng Lu, Pengda Li, Chon Him Choy, Zhuolei Yuan, Yanting Zhong, Chang-An Geng, et al.were lysed in RIPA buffer (Beyotime #P0013B) supplemented with a protease inhibitor cocktail (MCE #HY-K0010) and phosphatase inhibitors (Selleck #B15001). The resulting cell lysates were mixed with SDS loading

(14) AS1842856 Reduces β-Amyloid Burden via Inhibiting PLA2G4A-Mediated Lysosomal Dysfunction in APP/PS1 MiceCNS Neurosci TherApril 28, 2026Da-Long He, Zheng Wu, Rong-Jun Jia, Ting-Yao Wu, Yi-Min Qiu, Yong-Gang FanRIPA (Beyotime Biotechnology, P0013C) containing 1% PMSF (MCE, HY‐B0496), 1% protease inhibitor (MCE, HY‐K0010), and 1% phosphatase inhibitor (MCE, HY‐K0022). The lysate was centrifuged at 10,000 rpm for 20 min

(15) Lumican–TLR4 interaction accelerates hepatic fibrosis by activating hepatic stellate cellsHepatol CommunApril 24, 2026Yongqiang Xiong, Huanhuan Zhang, Ao Wang, Yaqian Cui, Jiaxi Ye, Leiming Jin, Mengsha Lin, Qianhui Zhang, Guang Liang, Wu Luo, et al.http://links.lww.com/HC9/C327. Western blot analysis RIPA buffer (Cat: P0013B, Beyotime) containing a protein inhibitor cocktail (Cat: HY-K0010; MCE, NJ, USA) was used to prepare liver tissue and cell lysates. The total protein extract was separated

(16) Intron polymorphism in Camk2d is associated with ventricular arrhythmias in normal adult Sprague-Dawley ratsExperimental AnimalsApril 22, 2026Rong LUO, Chunyun ZHAO, Yi WANG, Yilin HE, Chang LIU, Xiaoping LI, Xin CAO(BL-504A, Biosharp Biotechnology, Hefei, China) containing a complete protease inhibitor cocktail (HY-K0010, MedChemExpress, Monmouth Junction, NJ, USA) and phosphatase inhibitor (P1082, Beyotime Biotechnology,

(17) Thioamide Compound H0802 Enhances Hypoxia Tolerance by Mimicking Hypoxia-Adaptive Reprogramming of Glucose and Oxygen MetabolismAntioxidants (Basel)April 22, 2026Lehua Yin, Zhehan Liu, Yiran Li, Lei Li, Xiheng Li, Xingxing Yang, Jinyan Zhang, Shaoyi Huang, Hao Sun, Xu Yan, et al.X-100, 1% sodium deoxycholate, 0.1% SDS; pH 7.4) containing a protease inhibitor cocktail (MCE, Cat. #HY-K0010). The cell lysate was subjected to protein quantification using the Bradford assay (Bio-Rad (Hercules,

(18) Dual Pathways of UBE4B Inhibit Apoptosis in p53-Positive Tumor Cells via CCAR2 DegradationInt J Mol SciApril 21, 2026Bo Jin, Junyao Qu, Peng Xu, Bo Zhao, Xianting Jiao(Sangon Biotech, Shanghai, China, C500005-0100) supplemented with 100 × Protease Inhibitor Cocktail (MCE, HY-K0010), 100× Phenyl Methane Sulfonyl Fluoride (PMSF, Beyotime, Shanghai, China, ST507), and 100× Phosphatase

(19) Chicken infectious anemia virus exploits host CK2α as an essential factor for its replication via VP2 Ser182/Asp183-mediated interactionJournal of VirologyApril 21, 2026Ziyue Ma, Hongnuan Wang, Yongqiang Wang, Xiaoqi Li, Hong Cao, Li Gao, Shijun J. Zheng(Waltham, MA, USA). CHX (HY-12320), MG132 (HY-13259), CQ (HY-17589A), and protease inhibitor cocktail (HY-K0010) were purchased from MedChemExpress (China). Plasmid construction Chicken CK2α (GenBank accession

(20) Ectopic expression of Aspergillus flavus uricase and URAT1 in therapeutic cells promotes intracellular degradation of uric acid in hyperuricemic micePLoS OneApril 20, 2026Yuzhong Feng, Jiazhen Cui, Xuan Huang, Yupeng Li, Haolong Dong, Xianghua Xiong, Gang Liu, Qingyang Wang, Huipeng Chentransfected cells using RIPA lysis buffer supplemented with 1% protease inhibitor cocktail (MedChemExpress, HY-K0010), followed by centrifugation at 12,000 rpm, 4℃ for 15 min. The supernatant was separated by SDS-PAGE

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Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) from MedChemExpress

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Protease Inhibitor Cocktail (EDTA-Free, 100× in DMSO) from MedChemExpress

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