PMSF from MedChemExpress

Product Specs
ItemPMSF
CompanyMedChemExpress
Price $61.00Supplier Page View Company Product Page
Catalog NumberHY-B0496
Quantity10 mM * 1 mL
ApplicationsBioactivity, Structure and function analysis, ELISA, SDS-PAGE, Western blotting
Purity99.32%
TypeChemical
Molecular Weight174.19
Molecule NamePMSF
FormulaC7H7FO2S
Research AreaNeurological Disease

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(1) AS1842856 Reduces β-Amyloid Burden via Inhibiting PLA2G4A-Mediated Lysosomal Dysfunction in APP/PS1 MiceCNS Neurosci TherApril 28, 2026Da-Long He, Zheng Wu, Rong-Jun Jia, Ting-Yao Wu, Yi-Min Qiu, Yong-Gang Fanor mouse brain tissues were lysed in RIPA (Beyotime Biotechnology, P0013C) containing 1% PMSF (MCE, HY‐B0496), 1% protease inhibitor (MCE, HY‐K0010), and 1% phosphatase inhibitor (MCE, HY‐K0022). The lysate was

(2) Apolipoprotein E (APOE) Promotes Cell Proliferation and Invasion in Glioma via the PI3K/AKT Signaling PathwayCancer Manag ResApril 1, 2026Mengdi Xia, Ke Chen, Weiwei Chen, Zhenhua Ren, Xiaohui LiWestern Blotting The cells subjected to various treatments were lysed via RIPA buffer supplemented with PMSF (# HY-B0496, MedChemExpress) (1:100) and proteinase inhibitor cocktail (#HY-K00010, MedChemExpress) (1:100). Nuclear

(3) Apolipoprotein E (APOE) Promotes Cell Proliferation and Invasion in Glioma via the PI3K/AKT Signaling PathwayCancer Manag ResApril 1, 2026Mengdi Xia, Ke Chen, Weiwei Chen, Zhenhua Ren, Xiaohui LiWestern Blotting The cells subjected to various treatments were lysed via RIPA buffer supplemented with PMSF (# HY-B0496, MedChemExpress) (1:100) and proteinase inhibitor cocktail (#HY-K00010, MedChemExpress) (1:100). Nuclear

(4) Ginsenoside Rg3 Mitigates LPS-Induced Injury in Human Bronchial Epithelial Cells by Restoring Autophagic Flux and Inhibiting the TLR4/NF-κB-Mediated Inflammatory ResponseJ Inflamm ResJanuary 20, 2026Xingyu Tao, Lingjiao Liu, Xiaoke Gu, Baohui Jia, Qi Yu, Yingying Wei, Wei Zhang, Jing Zhou(Millipore, Cat# 20–188), protease inhibitor cocktail (Roche, Cat# 04693159001), PMSF (MedChemExpress, Cat# HY-B0496), BCA protein assay kit (Thermo Fisher, Cat# 23225), 5× SDS loading buffer (Solarbio, Cat# P1040), acrylamide

(5) Ginsenoside Rg3 Mitigates LPS-Induced Injury in Human Bronchial Epithelial Cells by Restoring Autophagic Flux and Inhibiting the TLR4/NF-κB-Mediated Inflammatory ResponseJournal of Inflammation ResearchJanuary 20, 2026Xingyu Tao, Lingjiao Liu, Xiaoke Gu, Baohui Jia, Qi Yu, Yingying Wei, Wei Zhang, Jing Zhou(Millipore, Cat# 20–188), protease inhibitor cocktail (Roche, Cat# 04693159001), PMSF (MedChemExpress, Cat# HY-B0496), BCA protein assay kit (Thermo Fisher, Cat# 23225), 5× SDS loading buffer (Solarbio, Cat# P1040), acrylamide

(6) Palmitic acid-triggered B7H3 palmitoylation promotes immune escapeNature CommunicationsJanuary 19, 2026Zejun Rao, Changsheng Huang, Qi Wu, Mao Li, Anyi Liu, Tong Zhu, Wangshuo Yang, Lanlan Yin, Shengyu Zhu, Xiaowei She, et al.μL per 1 × 106 cells in 100 μL. The reagents used as follows: phenylmethylsulfonyl fluoride (PMSF) (HY-B0496; MCE), phosphatase inhibitor cocktail I (HY-K0021; MCE), phosphatase inhibitor cocktail II (HY-K0022;

(7) Multidimensional mapping of stimulation-responsive regulatory elements and candidate causal variants in T cell activationSci AdvJanuary 1, 2026Zhao Wang, Wei Dong, Xiangling Feng, Xinran Xu, Yao An, Huiling Xiao, Menghan Luo, Qian Liang, Ruoxuan Bai, Ke Zhao, et al.supplemented with 1× protease inhibitor cocktail (MCE, HY-K0010) and phenylmethanesulfonyl fluoride (MCE, HY-B0496). The lysates were incubated on ice for 40 min with gentle mixing every 8 min. Following incubation,

(8) DHCR7 Promotes Liver Metastasis of Pancreatic Cancer Through PI3K ‐Akt Signaling PathwayCancer ScienceNovember 1, 2025Yu Xie, Ziyue Zhou, Jiangwei Song, Chengzhi Wang, Yibing Ding, Yudong Qiu, Lei Fang, Hao Chenglysis (AR0105, Beyotime, China) which contains Protease Inhibitor Cocktail (HY‐K0011, MCE, USA), PMSF (HY‐B0496, MCE, USA) and Phosphatase Inhibitor Cocktail I (HY‐K0021, MCE, USA), cells were incubated for 10 min,

(9) Lithium chloride-treated human umbilical cord mesenchymal stem cells-derived exsomes promote hair growth by regulating miR-146a-5p-wnt pathwayStem Cells Transl MedOctober 28, 2025Zhao Wang, Zeyu Chai, Xuegang Xu, Yongjun Piao, Lin Gongperformed using RIPA buffer (cat#HY-K1001, MedChemExpress) supplemented with a protease inhibitor (cat#HY-B0496, MedChemExpress) and a phosphatase inhibitor cocktail (cat#HY-K0021, MedChemExpress). Protein concentration

(10) Congenital Hypothyroidism Dysregulates TRPC6 to Mediate Abnormal Dendritic Spine Growth of Hippocampal NeuronsCNS Neuroscience & TherapeuticsSeptember 25, 2025Tianci Li, Feifei Shen, Lingling Li, Peng Chen, Zhiwen Zhu, Yuqin Zheng, Haiying Li, Guihai Suo, Yongjun Wang, Jinlong Shi, et al.were extracted from the cells with RIPA lysis buffer (MCE, Cat# HY‐K1001) containing PMSF (MCE, Cat# HY‐B0496). The primary neurons were scraped with a cytoskeletal scraper on ice, vortexed for 30 min at 4°C, and

(11) Inhibition of XPOT promotes breast cancer cell pyroptosis to suppress cancer progressionCentral European Journal of ImmunologySeptember 24, 2025Meng Liang, Min Wang, Beibei Zhuwith RIPA lysate (Yeasen, 20101ES60, China) containing 1% phenylmethanesulfonyl fluoride (PMSF; MCE, HY-B0496, USA) on ice. Lysates were collected and centrifuged to obtain protein samples. Protein concentration

(12) Targeted Blockage of Pathological Extracellular Vesicles and Particles From Fibroblast-Like Synoviocytes for Osteoarthritis Relief: Proteomic Analysis and Cellular EffectJournal of extracellular vesiclesSeptember 1, 2025Liu B, Xian Y, Shen T, Ben Y, Wu W, Shi Y, An X, Peng R, Gao W, Gong W, et al.supplemented with protease inhibitor cocktail, phosphatase inhibitors and PMSF (HY‐K1001, HY‐K0010 and HY‐B0496, MedChemExpress). Cells were lysed on ice for 15 min with periodic vortexing and detached from the culture

(13) Protocol for elucidating metabolite binding and regulation of TET2 dioxygenaseSTAR ProtocAugust 6, 2025Shuyuan Zhang, Zhou-Li Cheng, Dan YeBiotech,CAT#: A600153 Pepstatin A,APExBIO,CAT#: A2571 Leupeptin,APExBIO,CAT#: A2570 PMSF,MCE,CAT#: HY-B0496 His60 Ni Superflow resin,TAKARA,CAT#: 635659 10× T4 DNA ligase reaction buffer,NEB,CAT#: B0202S Dynabeads MyOne streptavidin C1

(14) Cancer-associated fibroblast-derived extracellular vesicles facilitate metastasis in hepatocellular carcinoma by delivering CTGFCell Oncol (Dordr)July 1, 2025Mengli Zheng, Luyao Liu, Haochen Cui, Yuchong Zhao, Wei Chen, Shuya Bai, Wang Peng, Yun Wang, Yanling Li, Ronghua Wang, et al.containing protease inhibitor cocktail (MCE, Cat#HY-K0021) and phenylmethylsulfonyl fluoride (MCE, Cat#HY-B0496). Protein concentration was determined using a BCA assay kit. Protein samples were boiled with 5 × SDS-PAGE

(15) Serine/threonine/tyrosine kinase 1 drives pancreatic carcinogenesis via GSK3β sequestration-mediated Wnt/β-catenin pathway hyperactivationSignal Transduct Target TherJune 30, 2025Cefan Zhou, Xueying Dong, Shi Li, Yue Xi, Yuan Liu, Xuehong Qian, Ziyan Song, Li Zhou, Rui Zhang, Hao Lyu, et al.proteinase inhibitor cocktail (Biomake, B14001) and 1 mM phenylmethylsulfonyl fluoride (PMSF, MCE, HY-B0496). Western blot was performed as described previously.20 The following primary antibodies were used:

(16) Pathway-based cancer transcriptome deciphers a high-resolution intrinsic heterogeneity within bladder cancer classificationJ Transl MedJune 17, 2025Zhan Wang, Zhaokai Zhou, Shuai Yang, Zhengrui Li, Run Shi, Ruizhi Wang, Kui Liu, Xiaojuan Tang, Qi Li(HY-K1001, MCE, China) supplemented with a 1% protease inhibitor cocktail (HY-K0010, MCE, China) and PMSF (HY-B0496, MCE, China). Protein concentration was determined using a BCA Protein Assay Kit (E162-01, GenStar,

(17) Protocol for high-throughput screening of SIRT7 inhibitors using fluorescent peptide technologySTAR ProtocolsJune 1, 2025Tian-Shu Kang, Xiaopeng Lu, Jinke Gu, Yuan Tian, Wei-Guo Zhu(LB) broth,Solarbio,Cat#L8291 Dimethyl sulfoxide (DMSO),Solarbio,Cat#D8371 Dithiothreitol (DTT),Beyotime,Cat#ST041 PMSF,MCE,Cat#HY-B0496 Protease inhibitor cocktail,MCE,Cat#HY-K0011 Sodium chloride (NaCl),Macklin,Cat#S805281 Magnesium

(18) Piezo1 regulates actin cytoskeleton remodeling to drive EMT in cervical cancer through the RhoA/ROCK1/PIP2 signaling pathwayDiscov OncolMay 16, 2025Juexiao Deng, Yang Li, Lanyue Zhang, Wenxin Liao, Tingting Liu, Fujin ShenP0013B) containing 1% protease inhibitor and 1% phenylmethylsulfonyl fluoride (MCE, China, Cat. No. HY-B0496). Protein concentrations were determined using the BCA method. SDS-PAGE was used for protein separation,

(19) Mechanical Load‐Induced Upregulation of Talin2 through Non‐Canonical Deubiquitination of OTUB1 Drives Facet Joint Osteoarthritis PathogenesisAdvanced ScienceApril 25, 2025Yizhen Huang, Heng Sun, Haojie Chen, Xiangpeng Wang, Junduo Zhao, Yang Jiao, Hongyi Zhou, Haoyu Cai, Jiafeng Dai, Xuan Huang, et al.radioimmunoprecipitation assay lysis buffer (HY‐K1001, MedChemExpress, Shanghai, China), phenylmethylsulphonyl fluoride (HY‐B0496, MedChemExpress), and 4× SDS‐PAGE sample loading buffer (HY‐K1100, MedChemExpress). Proteins were separated

(20) Retinoic acid enhances γδ T cell cytotoxicity in nasopharyngeal carcinoma by reversing immune exhaustionCell communication and signaling : CCSMarch 29, 2025Liu G, Quan Q, Pan L, Duan H, Zhang G, Li K, Zhu X, Zhang D, Li P, Zhao J.buffer containing Phosphatase Inhibitor Cocktail (Selleck, B15001) and 1 mM Protease Inhibitor (MCE, HY-B0496) on ice for 35 min. Protein supernatants were collected for further analysis. Total protein (20–40 μg)

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PMSF from MedChemExpress

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