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Protein Biochemistry
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Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH
2
O)
Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH
2
O) from MedChemExpress
Be the first to write a review!
Citations:
(133)
Supplier Page
Supplier Page from
MedChemExpress for
Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH
2
O)
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Description
Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH
2
O) is a blend of 5 phosphatase inhibitors that can broadly inhibit acid phosphatases, alkaline phosphatases, and protein tyrosine phosphatases (PTPs)
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH
2
O)
Company
MedChemExpress
Price
$38.00
Supplier Page
View Company Product Page
Catalog Number
HY-K0022
Quantity
1 mL
Applications
Bioactivity, Structure and function analysis, ELISA, SDS-PAGE, Western blotting
Type
kit
Add to Compare List
MedChemExpress
1 Deer Park Drive, Suite F
MONMOUTH JUNCTION
,
NJ
08852
United States
Phone
: US Tel: 609-228-6898
US Fax: 609-228-5909
EU Tel: +4686500910
Email: sales@medchemexpress.com
eu.sales@MedChemExpress.com
Company Profile
Website:
medchemexpress.com
Citations (133)
(1)
AS1842856 Reduces β-Amyloid Burden via Inhibiting PLA2G4A-Mediated Lysosomal Dysfunction in APP/PS1 Mice
CNS Neurosci Ther
April 28, 2026
Da-Long He, Zheng Wu, Rong-Jun Jia, Ting-Yao Wu, Yi-Min Qiu, Yong-Gang Fan
1%
PMSF
(
MCE
,
HY‐B0496
), 1% protease
inhibitor
(
MCE
,
HY‐K0010
), and 1% phosphatase
inhibitor
(
MCE
,
HY‐K0022
). The lysate was centrifuged at 10,000 rpm for 20 min at 4°C. The supernatant was collected, and the
(2)
Characterizing Response to PARP Inhibitor Treatment Combinations in Advanced Prostate Cancer
Biomedicines
April 22, 2026
Bryan Correa Gonzalez, Akshaya Karthikeyan, Love A. Moore, Anamitra Bhaumik, Ethan Sandoval, Marion Hardy, John D. McPherson, Hong Li, Mamta Parikh, Marc Dall’Era,
et al.
560767.AD), 1X
Phosphatase Inhibitor Cocktail II
(
MedChemExpress
, Monmouth Junction, NJ, USA, Cat#:
HY-K0022
), and 1X Halt
Protease Inhibitor Cocktail
with 5 mM
EDTA
(
ThermoFisher
, Waltham, MA, USA, Cat#:
78429
).
(3)
A Novel Small-Molecule TLR7 Agonist AXC-715 Stabilizes TLR7 Dimerization and Exhibits Broad-Spectrum Antiviral Activity
Microorganisms
April 11, 2026
Chen Yao, Meng-Hua Du, Yan-Jie Ma, Heng Wang, Liu Hang, Zhi-Cheng Li, Hong-Yu Yang, Guo-Yu Yang, Meng-Di Wang, Sheng-Li Ming
0.1% SDS, and 2 mM MgCl2, supplemented with protease and phosphatase
inhibitor
cocktails (
HY-K0010
,
HY-K0022
;
MedChemExpress
, Monmouth Junction, NJ, USA). Protein concentrations were determined using a
BCA Protein Assay Kit
(4)
CLOCK-catalyzed histone H3K37 glutarylation suppresses H3K36 trimethylation pathways in glioblastoma
Sci Adv
March 27, 2026
Yu Wang, Runxin Zhou, Xiao Zeng, Dingyuan Guo, Nan Li, Shuangli Li, Xu Zhang, Mingxuan Shi, Li Jiang, Mingzhu Fan,
et al.
phosphatase inhibitor cocktail I (catalog no.
HY-K0021
),
phosphatase inhibitor cocktail II
(catalog no.
HY-K0022
), deacetylase
inhibitor
cocktail (catalog no.
HY-K0030
), and protease
inhibitor
cocktail (catalog no.
(5)
Betrixaban activates cGAS and ERVs to promote dual nucleic-sensing antiviral immunity
EMBO Molecular Medicine
March 23, 2026
Xingyu Chen, Yang Zhao, Yunfei Xie, Tianyi Liu, Haocheng Wang, Xiao Wang, Xuefei Guo, Fuping You
Protease Inhibitor Cocktail,
MedChemExpress
,
HY-K0010
Phosphatase Inhibitor Cocktail II
,
MedChemExpress
,
HY-K0022
Phosphatase Inhibitor Cocktail III
,
MedChemExpress
,
HY-K0023
Nitrocellulose membrane,Beyotime,FFN08 Enhanced
(6)
USP25 regulates atherosclerosis by restricting RIPK1-mediated inflammatory responses
EBioMedicine
March 19, 2026
Xian Su, Bincheng Zhou, Yanqi Xu, Xue Du, Jiaqing Chen, Maojin Yin, Xiaomin Miao, Ying Zhao, Deqi Wang, Zhenhu Zhu,
et al.
cocktail (Cat#:
HY-K0010
,
MedChemExpress
, New Jersey, USA),
Phosphatase Inhibitor Cocktail II
(Cat#:
HY-K0022
,
MedChemExpress
), and
PMSF
(Cat#: P0100, Solarbio). Cell or tissue lysates were centrifuged at 12,000
(7)
Neuroprotective response against the onset of ischemic stroke by upregulation of histone H3Y99 sulfation
Cell Rep Med
March 17, 2026
Li Jiang, Junchang Xie, Jianfeng Wang, Yili Yi, Runxin Zhou, Dingyuan Guo, Yu Wang, Xiao Zeng, Mingxuan Shi, Jianing Ding,
et al.
Phosphatase inhibitor cocktail I
,
MCE
Chemexpress,Cat#
HY-K0021
Phosphatase inhibitor cocktail II
,
MCE
Chemexpress,Cat#
HY-K0022
Protease inhibitor cocktail
,
MCE
Chemexpress,Cat#
HY-K0010
Deacetylase inhibitor cocktail,
MCE
Chemexpress,Cat#
(8)
Deciphering Stiffness-Driven Changes in Colorectal Cancer by Proteomics
Molecular & Cellular Proteomics
March 1, 2026
Charlotte Cresens, Ana Montero-Calle, Guillermo Solís-Fernández, Behrad Shaghaghi, Lotte Gerrits, Samet Aytekin, Paul H.J. Kouwer, Rodrigo Barderas, Susana Rocha
(
RIPA buffer
,
Sigma-Aldrich
,
R0278
), with
1:100
v/v of proteases and phosphatases
inhibitor
cocktails (
MCE
,
HY-K0022
and
HY-K0010
), followed by
centrifugation
(10 min at 10,000 g, 4 °C) to remove cell debris. Protein
(9)
TFE3 fusion proteins drive TFE3 rearranged renal cell carcinoma progression via PGC-1α-mediated fatty acid oxidation
Frontiers in Immunology
February 4, 2026
Fan Feng, Yanhao Xu, Zhenggen Deng, Xiang Dong, Guijuan Chen, Wenliang Ma, Dongmei Li, Weidong Gan
(Beyotime, P0013C) supplemented with
protease inhibitors
(
MCE
,
HYK0010
) and phosphatase
inhibitors
(
MCE
,
HY-K0022
), while keeping the samples on ice. Protein concentration was determined using a
BCA Protein Assay Kit
(10)
Arginase 2 Promotes Colorectal Cancer Metastasis via PI3K/AKT Pathway Activation and Regulates Tumor Immune Infiltration
Cancer Med
February 3, 2026
Yueyan Zhang, Ning Qu, Jiale Mei, Caixia Mo, Luojuan Wei, Haixian Shen, Shanfei Zhou, Jinmin Hu, Wenqi Luo, Xianwei Mo
supplemented with
protease inhibitors
(
HYK0010
,
MCE
) and phosphatase
inhibitors
I (
HY‐K0021
,
MCE
) and II (
HY‐K0022
,
MCE
) to extract total protein. For tissue samples, frozen CRC or adjacent normal tissue was homogenized
(11)
Senescence-induced endothelial-to-mesenchymal transition accelerates the subretinal fibrosis in neovascualr age-related macular degeneration
Journal of Translational Medicine
January 22, 2026
Yinhao Wang, Huiling Ma, Jiayun Ge, Kuangqi Chen, Kai Chen, Hui Ye, Xinlei Pan, Xinglin Wang, Jianhua Xia, Jianqin Shen,
et al.
P1010, Shanghai, China) and
phosphatase inhibitor cocktail I
/II (
MedChemExpress
, Cat. No.
HY-K0021
/
HY-K0022
, USA). Protein concentration was quantified via
BCA
assay
(Beyotime, Cat. No. PP102-01, Shanghai, China).
(12)
Palmitic acid-triggered B7H3 palmitoylation promotes immune escape
Nature Communications
January 19, 2026
Zejun Rao, Changsheng Huang, Qi Wu, Mao Li, Anyi Liu, Tong Zhu, Wangshuo Yang, Lanlan Yin, Shengyu Zhu, Xiaowei She,
et al.
(
HY-B0496
;
MCE
),
phosphatase inhibitor cocktail I
(
HY-K0021
;
MCE
),
phosphatase inhibitor cocktail II
(
HY-K0022
;
MCE
), chloroquine (
HY-17589A
;
MCE
)
2-bromohexadecanoic acid
(
HY-111770
;
MCE
), palmostatin B (HY-120911;
(13)
Mesenchymal stromal cells counteract with age-related immune decline and enhance vaccine efficacy by modulating endogenous splenic marginal reticular cells in elderly models
Cellular & Molecular Immunology
January 9, 2026
Jialing Liu, Zhishan Li, Qiong Ke, Qiuli Liu, Yueming Sun, Rong Yan, Huolin Ye, Yuxi Zhang, Jie Ren, Hong Chen,
et al.
phenylmethylsulfonyl fluoride (PMSF, Roche, CAS: 329-98-6) and a phosphatase
inhibitor
cocktail (
MedChemExpress
, Cat#:
HY-K0022
) for
protein extraction
. The protein concentration was determined via the
Pierce™ BCA Protein Assay Kit
(14)
Plant Virus‐Induced Inheritable Apoptosis Drives Reproductive Costs in Female Insect Vectors to Balance Viral Biparental Transmission
Advanced Science
January 1, 2026
Haibo Wu, Wenqiang Wan, Qingquan Liang, Hengsong Yang, Chengcong Lu, Taiyun Wei, Qian Chen
Switzerland; 04693132001) and phosphatase
inhibitor
cocktail (
MedChemExpress
, Monmouth Junction, NJ, USA;
HY‐K0022
). The lysates were separated by
SDS‐PAGE
using gels including Phos‐tag acrylamide (Wako, Osaka, Japan;
(15)
Targeting the FOSL1/IKKα positive feedback loop attenuates glioblastoma malignancy via suppression of NF-κB signaling
Theranostics
January 1, 2026
Beichen Zhang, Hao Tian, Haoyu Zhou, Yichang Wang, Ke Gao, Yiyang Cao, Mingjing Zhou, Maode Wang, Wei Wu, Jia Wang
supplemented with protease
inhibitor
cocktail (
HY-K0010
,
MCE
) and phosphatase
inhibitor
cocktails (
HY-K0022
,
HY-K0023
,
MCE
). Cytoplasmic and nuclear protein fractions were isolated using the Nuclear and Cytoplasmic
(16)
Porcine reproductive and respiratory syndrome virus activates the pentose phosphate pathway via the ROS/HIF-1α/G6PD axis to promote viral replication
Virulence
December 31, 2025
Ying-Xian Ma, Jia-Ming Yang, Hang-Tian Mei, Lei Zeng, Guo-Yu Yang, Jiang Wang, Sheng-Li Ming, Bei-Bei Chu
analysis
Cell lysis
used ice-cold RIPA buffer (P0013B, Beyotime) containing
protease inhibitors
(
HY-K0010
,
HY-K0022
;
MedChemExpress
). Lysates/supernatants mixed with SDS loading buffer were denatured (98°C, 10 min).
(17)
Single-cell transcriptomic profiling reveals aberrant CD4⁺ naive T cell differentiation driving immune activation in Behçet’s uveitis
Journal of Translational Medicine
December 2, 2025
Lu Zhang, Yimeng Sun, Xinyu Yao, Chun Yan, Yunfei Liu, Dijie Qiao, Ziyu Du, Cong Hu, Fuying Guo, Ziwei Meng,
et al.
(Beyotime, Cat#P0013B) supplemented with protease (
MCE
, Cat#
HY-K0010
) and phosphatase
inhibitors
(
MCE
, Cat#
HY-K0022
and
HY-K0023
), followed by
centrifugation
at 12,000 × g for 15 min at 4 °C. Equal amounts of protein
(18)
Tumoral ALOX5 mediated arachidonic acid metabolism regulates immune response in non-small cell lung cancer
Cellular oncology (Dordrecht)
December 1, 2025
Gao Y, Xia Y, Li J, Lai Y, Yin S, Lin J, Yu D, Huang S, Lu F, Wang W,
et al
.
inhibitor
cocktail (
MedChemExpress
,
HY-K0010
) and phosphatase
inhibitor
cocktails (
MedChemExpress
,
HY-K0022
,
HY-K0023
). The lysate was centrifuged at 13,000 × g for 15 min at 4 °C. Protein concentration in the
(19)
Aurora kinase B phosphorylates ZBP1 to drive PANoptosis following treatment with PARP and ATR inhibitors combination
Nature Communications
November 27, 2025
Wenjian Gong, Zhiqi Liao, Fan Xiong, Linghui Wang, Shennan Shi, Mengshi Luo, Yuewen Zhang, Qiuyang Xu, Yuanyuan Wang, Yijie Wu,
et al.
HY-K0010
) and
phosphatase inhibitor cocktail I
(
HY-K0021
) and
phosphatase inhibitor cocktail II
(
MCE
,
HY-K0022
). After
incubation
at 4 °C for 30 min, the lysates were sonicated for 30 s and clarified by
centrifugation
(20)
Glucose starvation mimetic aldometanib removes immune barriers permitting mice with hepatocellular carcinoma to live to normal ages
Cell Research
November 25, 2025
Hui-Hui Hu, Xuefeng Wang, Bin Lan, Haili Cheng, Hong Wen, Fangfang Chen, Jianfeng Wu, Mengqi Li, Jiazhou Chen, Jinhui Zhang,
et al.
New England Biolabs
.
Phosphatase Inhibitor Cocktail I
(Cat#
HY-K0021
),
Phosphatase Inhibitor Cocktail II
(Cat#
HY-K0022
),
Protease Inhibitor Cocktail
(Cat#
HY-K0010
), and DAPI (Cat#
HY-D0814
) were purchased from
MedChemExpress
.
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Reviews
Be the First to Write a Review!
Phosphatase Inhibitor Cocktail Ⅱ (100× in ddH
2
O) from MedChemExpress
Supplier Page
View Company Product Page
$38.00
Product Specs
Company Info
Citations
Reviews
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