NEBuffer 3.R Buffer from New England Biolabs

Product Specs
ItemNEBuffer 3.R Buffer
CompanyNew England Biolabs
Price $27.00 Inquire
Catalog NumberB6003S
Temperature-20 C
ApplicationsDNA Manipulation
TypeBuffer
Quantity5 ml

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A spatial multi-omics atlas of immunosenescence reveals germinal-center B cell dysfunction in human lymph nodesCell Press BlueJuly 20, 2026Negin Farzad, Archibald Enninful, Yao Lu, Fabio Parisi, Anthony A. Fung, Yumi Kwon, Yajuan Li, Margaux Labrosse, Mingyu Yang, Francesco Strino, et al.,Thermo Fisher Scientific,EP0753 dNTP Mix,Thermo Fisher Scientific,R0192 NEBuffer 3.1,New England Biolabs,B6003S T4 DNA ligase,New England Biolabs,M0202L SDS,Sigma-Aldrich,71736-500ML "Proteinase K, recombinant, PCR grade

(2) Spatial Glyco-Codes Define Human Liver Pathology and ProgressionbioRxivJuly 12, 2026Xiaolong Tian, Anthony Fung, Xingbo Shang, Dingyao Zhang, Binfan Chen, Lei Zhang, Keyi Li, Mei Zhong, Yifan Deng, Mingyu Yang, et al.Formaldehyde (37% by Weight/Molecular Biology),Fisher Scientific,BP531-25 NEBuffer™ r3.1,New England Biolabs,B6003S E.coli Poly (A) Polymerase,New England Biolabs,M0276L Cell Staining Buffer,BioLegend,420201 DNA LoBind®

(3) The molecular asynchrony of single cellsbioRxivJune 5, 2026Boshi Fu, Robert Tan, Zhenkun Cao, Xiuzhen Bai, Dongsheng Bai, Jinghui Song, Chenxu Zhubarcoding. Combinatorial barcoding. Nuclei pellets were resuspended and thoroughly dispersed in 1 mL 1× NEBuffer 3.1(NEB, B6003S). The homogenized nuclei suspension was then incorporated into a master ligation mix consisting of 2,262

(4) Nickase NmCas9 unsilences paternal Ube3a in a mouse model of Angelman syndrome without causing AAV vector integrationScientific ReportsMay 14, 2026Hannah O. Bazick, Lucas M. James, Mark J. Zylkaprotein lysate (produced above) with 200 ng of plasmid or PCR amplified substrate in NEBuffer r3.1 (B6003S, New England Biolabs) for 18 h at 37 °C. We then mixed samples with 6x gel loading dye (B7024S, New England Biolabs

(5) APE1 coordinates its disordered region and metal cofactors to drive genome surveillanceNucleic Acids ResMay 14, 2026Donghun Lee, Subin Kim, Gyeongpil Jo, Juwon Kim, Jungmin Yoo, Jejoong Yoo, Ja Yil Lee, Gwangrog Leeabasic site. Six units/ml of Nt.BspQI (R0644S, NEB) was treated to 10 nM of λ-I3 in 1× NEBuffer r3.1 (B6003S, New England Biolabs) with 1 mM ATP and then incubated at 50°C for 1 h. To inactivate nickases, proteinase

(6) Spatially Resolved in vivo CRISPR Screen Sequencing via Perturb-DBiTbioRxivMay 3, 2026Alev Baysoy, Xiaolong Tian, Feifei Zhang, Paul Renauer, Zhiliang Bai, Hao Shi, Haikuo Li, Bo Tao, Mingyu Yang, Archibald Enninful, et al.,Thermo Fisher Scientific,EP0753 dNTP Mix,Thermo Fisher Scientific,R0192 NEBuffer 3.1,New England Biolabs,B6003S T4 DNA ligase,New England Biolabs,M0202L SDS,Sigma-Aldrich,71736-500ML "Proteinase K, recombinant, PCR grade

(7) Plant-associated fungi co-opt ancient antimicrobials for host manipulationSci AdvApril 29, 2026Fantin Mesny, Valentina Wolf, Ana López-Moral, Anton Kraege, Wilko Punt, Saifei Liu, Jinyi Zhu, Jiyeun Park, Yukiyo Sato, Bart P. H. J. Thommatransformation. First, 4 μM of this Cas9 enzyme was mixed to 2× of NEBuffer r3.1 (New England Biolabs; ref. B6003S) and sgRNA (0.3 μg/μl). The mixture was incubated at 25°C for 30 min to bind sgRNA and enzyme. Both

(8) CUT&Tag for High-Resolution Epigenomic Profiling From a Low Amount of Arabidopsis TissuePlant DirectApril 10, 2026Yixuan Fu, Marc W. Schmid, Sara Simoniniplasmid at 250 ng/μL (we used a derivative of pAGM65879 [Addgene #153214]), 1 μL NEBuffer 3.1 (NEB #B6003S), 6.75 μL ddH2O, and incubated at 37°C for 15 min. To stop the reaction, 1 μL of 1% SDS (Roth #CN30.3)

(9) MIC-Drop-seq: scalable single-cell phenotyping of mutant vertebrate embryosNature CommunicationsApril 1, 2026Clayton M. Carey, Saba Parvez, Zachary J. Brandt, Brent W. Bisgrove, Christopher J. Yates, Randall T. Peterson, James A. Gagnoncontaining 200 ng/µL gRNAs, 3.36 µM EnGen Cas9 (NEB, cat. no. M0646M) and 1x NEB buffer r3.1 (NEB, cat. no. B6003S) was assembled and incubated at room temperature for 5-10 min. 3.5 µL of 0.5% phenol red dye (Sigma-Aldrich,

(10) Protease-mediated PRC1 dissociation promotes H2AK119ub remodeling during stress responsesThe EMBO JournalMarch 11, 2026Wei Cui, Qingyang Li, Jinsong Wei, Ting Zhou, Haozhe Zhu, Delai Huang, Shuai Wang, Jianan Gao, Ru Zhou, Zeyu Sun, et al.restriction enzyme,New England Biolabs,New England Biolabs NEBuffer™ r3.1,New England Biolabs,Cat# B6003S rCutSmart™ Buffer,New England Biolabs,Cat# B6004S RIPA,Beyotime Biotechnology,Cat# P0013B Cell lysis

(11) Haplotype editing with CRISPR-Cas9 as a therapeutic approach for dominant-negative missense mutations in NEFLMolecular TherapyMarch 4, 2026Poorvi H. Dua, Bazilco M.J. Simon, Chiara B.E. Marley, Carissa M. Feliciano, Hannah L. Watry, Quinn T. Cowan, Dylan Steury, Abin Abraham, Erin N. Gilbertson, Grace D. Ramey, et al.by addition of 1 mL of 10 μM Cas9 with 3 mL of 10 μM gRNA in 3 mL NebBuffer r3.1 (NEB, catalog no. B6003S) and 20 mL DNAse/RNase free water. This was incubated at room temperature for 10 min. Purified PCR amplicon

(12) In situ transplantation and multiple omics reveal holobiont adaptation in deep-sea mussel Gigantidas haimaensisiScienceFebruary 20, 2026Tong Wei, Guoyong Yan, Maeva Perez, Xing He, Wai Chuen Wong, Ting Xu, Yi Lan, Jin Sun, Pei-Yuan Qian,Thermo Fisher Scientific,Cat#J61196.AP Lysis Buffer,Thermo Scientific,Cat#PI87787 NEB Buffer,New England Biolabs,Cat#B6003S MboI Restriction Enzyme,New England Biolabs,Cat#R0147S Sodium Dodecyl Sulfate (SDS),Thermo Scientific,Cat#28312 Biotin-14-dCTP,Thermo Fisher Scientific

(13) Multichannel genomic recording of biological information with ENGRAMNature protocolsFebruary 11, 2026Nathans JF, McDiarmid TA, Chen W, Shendure J.(NEB, cat. no. R3539S)10× rCutSmart buffer (NEB, cat. no. B6004S)10× NEBuffer™ r3.1 (NEB, cat. no. B6003S)NEBridge Ligase Master Mix (NEB, cat. no. M1100S)T4 DNA ligase (NEB, cat. no. M0202S)10× T4 DNA ligase reaction buffer

(14) Integrative Single-Cell Epigenomic Atlas Annotates the Regulatory Genome of the Adult Mouse BrainbioRxivFebruary 7, 2026Zhaoning Wang, Songpeng Zu, Ethan J. Armand, Timothy H. Loe, Jonathan A. Rink, Wanying Wu, Yang Xie, Lei Chang, Chenxu Zhu, Nicholas D. Johnson, et al.equilibrated to room temperature before use. Post-RT nuclei were resuspended in 2 mL of 1x NEBuffer 3.1 (NEB, B6003S) and then transferred to the ligation mix [4500 μL H2O, 1000 μL 10x T4 Ligase Buffer (NEB, B0202S),

(15) Efficient CRISPR-Cas genome editing in brown algaeCell Reports MethodsJanuary 26, 2026Cláudia Martinho, Masakazu Hoshino, Morgane Raphalen, Viktoriia Bukhanets, Anagha Kerur, Kenny A. Bogaert, Rémy Luthringer, Susana M. CoelhoBuffer,IDT,Cat# 11-01-03-01 IDTE Buffer (pH 7.5),IDT,Cat# 11-01-02-02 NEBuffer r3.1,New England Biolabs,Cat# B6003S 2-fluoroadenine (2-FA),Sigma,Cat# 535087-1G Nuclease-free water,Ambion,Cat# AM9932 ,, Critical commercial

(16) Multi-targeting zinc finger nuclease vector unsilences paternal UBE3A in a mouse model of Angelman syndromeGene TherapyJanuary 1, 2026Hannah O. Bazick, Lucas M. James, Bonnie Taylor-Blake, Justin M. Wolter, Mark J. ZylkaWe then incubated protein lysate (produced above) with 200 ng of plasmid substrate in NEBuffer r3.1 (B6003S, New England Biolabs, Ipswich, MA, USA) for 18 h at 37 °C. Samples were then combined with 6x gel loading

(17) Pathways and products of base excision DNA repair in Xenopus laevis eggs: contrast with human cell pathwaysNucleic Acids ResDecember 10, 2025Zhouyiyuan Xue, Patrick James Sutton, John D Haley, Christopher W Brownlee, Bruce Demple100 pmol of N(3–1) Ver 1 X:A was nicked using 120 units of Nt.BspQI (NEB, R0644S) in NEB r3.1 (NEB, B6003S) at 50°C for 1 h in a total reaction volume of 500 μl. Subsequently, 100 units of Nb.BbvCI (NEB, R0631L)

(18) An inducible model of human post-implantation development derived from primed and naive stem cellsCell stem cellOctober 2, 2025Oura S, Li L, Wu J.,NEW ENGLAND BioLabs,R0104S NEBuffer r2.1,NEW ENGLAND BioLabs,B6002S NEBuffer r3.1,NEW ENGLAND BioLabs,B6003S PrimeSTAR GXL DNA Polymerase,Takara Bio,R050B Lipofectamin 3000 Transfection Reagent,Thermo Fisher Scientific

(19) The individual isoforms of ORMDL, the regulatory subunit of serine palmitoyltransferase, have distinctive sensitivities to ceramideBiochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsOctober 1, 2025Usha Mahawar, Deanna L. Davis, Muthukumar Kannan, John Suemitsu, Christopher D. Oltorik, Faheem Farooq, Raheema Fulani, Collin Weintraub, Binks Wattenberg(NEB #R0580), DTT (NEB #7061LM), calf intestinal alkaline phosphatase (NEB #M0290), NEB buffer 3 (NEB #B6003S), GE healthcare PCR DNA and gel purification kit as per their protocol (Cytiva, #28903470), Quick ligase

(20) Genetic and behavioral analyses suggest that larval and adult stages of Lucilia cuprina employ different sensory systems to detect rotten beefParasit VectorsJuly 9, 2025Juan P. Wulff, Rachel K. Laminack, Maxwell J. Scottwith the duplex crRNA::tracrRNA, 1 M potassium chloride (KCl) and 1 μl of 3.1 buffer (NEB, cat. no. B6003S) and incubated at 25 °C for 20 min to form the RNP complex. The mixture was cooled to room temperature

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