NEBuffer 3.R Buffer from New England Biolabs

Product Specs
ItemNEBuffer 3.R Buffer
CompanyNew England Biolabs
Price $27.00 Inquire
Catalog NumberB6003S
Temperature-20 C
ApplicationsDNA Manipulation
TypeBuffer
Quantity5 ml

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Plant-associated fungi co-opt ancient antimicrobials for host manipulationSci AdvApril 29, 2026Fantin Mesny, Valentina Wolf, Ana López-Moral, Anton Kraege, Wilko Punt, Saifei Liu, Jinyi Zhu, Jiyeun Park, Yukiyo Sato, Bart P. H. J. Thommatransformation. First, 4 μM of this Cas9 enzyme was mixed to 2× of NEBuffer r3.1 (New England Biolabs; ref. B6003S) and sgRNA (0.3 μg/μl). The mixture was incubated at 25°C for 30 min to bind sgRNA and enzyme. Both

(2) CUT&Tag for High-Resolution Epigenomic Profiling From a Low Amount of Arabidopsis TissuePlant DirectApril 10, 2026Yixuan Fu, Marc W. Schmid, Sara Simoniniplasmid at 250 ng/μL (we used a derivative of pAGM65879 [Addgene #153214]), 1 μL NEBuffer 3.1 (NEB #B6003S), 6.75 μL ddH2O, and incubated at 37°C for 15 min. To stop the reaction, 1 μL of 1% SDS (Roth #CN30.3)

(3) Protease-mediated PRC1 dissociation promotes H2AK119ub remodeling during stress responsesThe EMBO JournalMarch 11, 2026Wei Cui, Qingyang Li, Jinsong Wei, Ting Zhou, Haozhe Zhu, Delai Huang, Shuai Wang, Jianan Gao, Ru Zhou, Zeyu Sun, et al.restriction enzyme,New England Biolabs,New England Biolabs NEBuffer™ r3.1,New England Biolabs,Cat# B6003S rCutSmart™ Buffer,New England Biolabs,Cat# B6004S RIPA,Beyotime Biotechnology,Cat# P0013B Cell lysis

(4) Haplotype editing with CRISPR-Cas9 as a therapeutic approach for dominant-negative missense mutations in NEFLMolecular TherapyMarch 4, 2026Poorvi H. Dua, Bazilco M.J. Simon, Chiara B.E. Marley, Carissa M. Feliciano, Hannah L. Watry, Quinn T. Cowan, Dylan Steury, Abin Abraham, Erin N. Gilbertson, Grace D. Ramey, et al.by addition of 1 mL of 10 μM Cas9 with 3 mL of 10 μM gRNA in 3 mL NebBuffer r3.1 (NEB, catalog no. B6003S) and 20 mL DNAse/RNase free water. This was incubated at room temperature for 10 min. Purified PCR amplicon

(5) In situ transplantation and multiple omics reveal holobiont adaptation in deep-sea mussel Gigantidas haimaensisiScienceFebruary 20, 2026Tong Wei, Guoyong Yan, Maeva Perez, Xing He, Wai Chuen Wong, Ting Xu, Yi Lan, Jin Sun, Pei-Yuan Qian,Thermo Fisher Scientific,Cat#J61196.AP Lysis Buffer,Thermo Scientific,Cat#PI87787 NEB Buffer,New England Biolabs,Cat#B6003S MboI Restriction Enzyme,New England Biolabs,Cat#R0147S Sodium Dodecyl Sulfate (SDS),Thermo Scientific,Cat#28312 Biotin-14-dCTP,Thermo Fisher Scientific

(6) Integrative Single-Cell Epigenomic Atlas Annotates the Regulatory Genome of the Adult Mouse BrainbioRxivFebruary 7, 2026Zhaoning Wang, Songpeng Zu, Ethan J. Armand, Timothy H. Loe, Jonathan A. Rink, Wanying Wu, Yang Xie, Lei Chang, Chenxu Zhu, Nicholas D. Johnson, et al.equilibrated to room temperature before use. Post-RT nuclei were resuspended in 2 mL of 1x NEBuffer 3.1 (NEB, B6003S) and then transferred to the ligation mix [4500 μL H2O, 1000 μL 10x T4 Ligase Buffer (NEB, B0202S),

(7) Efficient CRISPR-Cas genome editing in brown algaeCell Reports MethodsJanuary 26, 2026Cláudia Martinho, Masakazu Hoshino, Morgane Raphalen, Viktoriia Bukhanets, Anagha Kerur, Kenny A. Bogaert, Rémy Luthringer, Susana M. CoelhoBuffer,IDT,Cat# 11-01-03-01 IDTE Buffer (pH 7.5),IDT,Cat# 11-01-02-02 NEBuffer r3.1,New England Biolabs,Cat# B6003S 2-fluoroadenine (2-FA),Sigma,Cat# 535087-1G Nuclease-free water,Ambion,Cat# AM9932 ,, Critical commercial

(8) Multi-targeting zinc finger nuclease vector unsilences paternal UBE3A in a mouse model of Angelman syndromeGene TherapyJanuary 1, 2026Hannah O. Bazick, Lucas M. James, Bonnie Taylor-Blake, Justin M. Wolter, Mark J. ZylkaWe then incubated protein lysate (produced above) with 200 ng of plasmid substrate in NEBuffer r3.1 (B6003S, New England Biolabs, Ipswich, MA, USA) for 18 h at 37 °C. Samples were then combined with 6x gel loading

(9) Pathways and products of base excision DNA repair in Xenopus laevis eggs: contrast with human cell pathwaysNucleic Acids ResDecember 10, 2025Zhouyiyuan Xue, Patrick James Sutton, John D Haley, Christopher W Brownlee, Bruce Demple100 pmol of N(3–1) Ver 1 X:A was nicked using 120 units of Nt.BspQI (NEB, R0644S) in NEB r3.1 (NEB, B6003S) at 50°C for 1 h in a total reaction volume of 500 μl. Subsequently, 100 units of Nb.BbvCI (NEB, R0631L)

(10) The individual isoforms of ORMDL, the regulatory subunit of serine palmitoyltransferase, have distinctive sensitivities to ceramideBiochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of LipidsOctober 1, 2025Usha Mahawar, Deanna L. Davis, Muthukumar Kannan, John Suemitsu, Christopher D. Oltorik, Faheem Farooq, Raheema Fulani, Collin Weintraub, Binks Wattenberg(NEB #R0580), DTT (NEB #7061LM), calf intestinal alkaline phosphatase (NEB #M0290), NEB buffer 3 (NEB #B6003S), GE healthcare PCR DNA and gel purification kit as per their protocol (Cytiva, #28903470), Quick ligase

(11) Genetic and behavioral analyses suggest that larval and adult stages of Lucilia cuprina employ different sensory systems to detect rotten beefParasit VectorsJuly 9, 2025Juan P. Wulff, Rachel K. Laminack, Maxwell J. Scottwith the duplex crRNA::tracrRNA, 1 M potassium chloride (KCl) and 1 μl of 3.1 buffer (NEB, cat. no. B6003S) and incubated at 25 °C for 20 min to form the RNP complex. The mixture was cooled to room temperature

(12) Protocol for Generation of Single-Gene Knockout in Hard-to-Transfect THP1 Cell Lines Using CRISPR/Cas9BIO-PROTOCOLJuly 5, 2025Kaveri Srivastava, Bhaswati Pandit(New England Biolabs, catalog number: R0739S) 22. r3.1 buffer (New England Biolabs, catalog number: B6003S) 23. TEMED (Sigma-Aldrich, catalog number: 1107320100) 24. cOmplete Mini (Sigma-Aldrich, catalog number:

(13) Toward optimizing diversifying base editors for high-throughput mutational scanning studiesNucleic Acids ResJuly 4, 2025Carley I Schwartz, Nathan S Abell, Amy Li, Aradhana, Josh Tycko, Alisa Truong, Stephen B Montgomery, Gaelen T Hessdigested for 8 h at 55°C [10 μg plasmid library, 9 μl BsmBI (NEB, R0739L), and 10 μl of Buffer 3.1 (NEB, B6003S)]. The resulting digest was analyzed on a 1% TAE agarose gel and purified. The stem-loop insert was

(14) DNA G-quadruplex structures act as functional elements in α- and β-globin enhancersGenome BiolJune 4, 2025Colm Doyle, Krzysztof Herka, Sean M. Flynn, Larry Melidis, Somdutta Dhir, Stefan Schoenfelder, David Tannahill, Shankar Balasubramaniancentrifuged at 1800 rpm for 5 min at 4 °C. Nuclei were overlayed once with 500 mL of 1.25 × NEBuffer r3.1 (B6003S, New England Biolabs); buffer was removed, pellet was resuspended in 1 mL of 1.25 × NEBuffer r3.1, and

(15) MIC-Drop-seq: Scalable single-cell phenotyping of mutant vertebrate embryosbioRxivMay 30, 2025Clayton M. Carey, Saba Parvez, Zachary J. Brandt, Brent W. Bisgrove, Christopher J. Yates, Randall T. Peterson, James A. Gagnoncontaining 200 ng/μL gRNAs, 3.36 μM EnGen Cas9 (NEB, cat. no. M0646M) and 1x NEB buffer r3.1 (NEB, cat. no. B6003S) was assembled and incubated at room temperature for 5–10 min. 3.5 μL of 0.5% phenol red dye (Sigma-Aldrich,

(16) CRISPR/Cas9 Knockout of Shell Matrix Protein 1 in the Slipper‐Snail Crepidula atrasoleaJournal of Experimental Zoology Part B: Molecular and Developmental EvolutionMay 4, 2025Grant Batzel, Yiqun Wang, Antonia Bock, Elbereth Chen, Stephanie Neal, Rebecca N. Lopez‐Anido, Yoon Lee, Evan Tjeerdema, Emily Ignatoff, Tejasvi Patil, et al.was incubated with 30 nM SpCas9 nuclease (M0386S, New England Biolabs) with or without NEBuffer r3.1 (B6003S, New England Biolabs) for 10 min at 25°C. Circular or linearized plasmid DNA (3 nM each) was added to

(17) Single-cell parallel analysis of DNA damage and transcriptome reveals selective genome vulnerabilityNature MethodsMay 1, 2025Dongsheng Bai, Zhenkun Cao, Nivedita Attada, Jinghui Song, Chenxu Zhubarcoding. Ligation-based combinatorial barcoding. Nuclei were resuspended in 1 mL of 1× NEBuffer r3.1 (NEB, B6003S) and transferred to a ligation mix containing: 2,262 μL H2O, 500 μL 10× T4 DNA Ligase Buffer (NEB, B0202S),

(18) The Hydractinia cell atlas reveals cellular and molecular principles of cnidarian colonialityNat CommunMarch 3, 2025David A. Salamanca-Díaz, Helen R. Horkan, Helena García-Castro, Elena Emili, Miguel Salinas-Saavedra, Alberto Pérez-Posada, Maria Eleonora Rossi, Marta Álvarez-Presas, Rowan Mac Gabhann, Paula Hillenbrand, et al.the RT/R1 barcoding plate (for a final concentration of 5000 cells per well) with NEBuffer r3.1 (NEB, B6003S), T4 Ligase Buffer 10× (NEB, M0202L), T4 DNA ligase (400 U/μL, NEB, M0202L). The addition of 6.5 μL

(19) Dynamic changes in 3D chromatin structure during male gametogenesis in Arabidopsis thalianaGenome BiolFebruary 10, 2025Zhihan Song, Qimin Xia, Minqi Yang, Tingting Yang, Yali Liu, Dingyue Wang, Jiayue Shu, Zhiyuan Liu, Yi Chi, Heming Xu, et al.generative nuclei were sorted into 200-μL tubes containing 2.5 μL 0.3% SDS buffer in NEBuffer r3.1 (B6003S, NEB). After gentle centrifugation, the mixture was incubated at 37 °C for 1 h, and then quenched with

(20) Pioneering genome editing in parthenogenetic stick insects: CRISPR/Cas9-mediated gene knockout in Medauroidea extradentataSci RepJanuary 20, 2025Giulia Di Cristina, Elina Dirksen, Benjamin Altenhein, Ansgar Büschges, Sigrun I. Korsching200 ng of the candidate sgRNA, 300 ng of EnGen® Spy Cas9 NLS (NEB M0646M) and 1X NEBuffer r3.1 (NEB B6003S). The digestion products were separated and visualized in a 2% agarose TAE (Tris-acetate-EDTA) gel.

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