NEBuffer 2.R Buffer from New England Biolabs

Product Specs
ItemNEBuffer 2.R Buffer
CompanyNew England Biolabs
Price $27.00 Inquire
Catalog NumberB6002S
Temperature-20 C
ApplicationsDNA Manipulation
TypeBuffer
Quantity5 ml

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) RAPID: evaluation of Cas12a protospacer nicking and chimeric reporters for PAM-independent RNA and DNA diagnosticsNucleic Acids ResAugust 4, 2026Idorenyin A Iwe, Frank X Liu, Ariel Corsano, Severino Jefferson Ribeiro da Silva, Jennifer Doucet, Serena Singh, Gabriel Lamothe, Riham Zayani, Jessica Nguyen, Quinn Matthews, et al.Lachnospiraceae bacterium, LbCas12a (#M0653T), Cas12 diluent (#B0653A), Cas12 reaction buffer (NEBuffer™ r2.1, #B6002S), and RNase inhibitor (#M0314L) were purchased from New England Biolabs (NEB). Magnesium chloride solution

(2) Toward point-of-care and amplification-free detection of human cytomegalovirus using CRISPR-Cas12aiScienceJuly 17, 2026Kavish A.V. Kohabir, A.W.J. Rietveld, Lars O. Nooi, Roos E. Beijer, Jeanne E. van Dongen, Jasper Linthorst, Rob M.F. Wolthuis, Marcel Jonges, Matthijs R.A. Welkers, Loes I. Segerink, et al.Industries Inc.,N/A rCutSmart® buffer,New England Biolabs,B6004S NEBuffer 2.1,New England Biolabs,B6002S AsCas12a Ultra,Integrated DNA Technologies Inc.,10001273 FluoSurf,Emulseo,P/N 3200808 ,, Critical

(3) Amplification-Free Detection of Highly Structured RNA Molecules Using SCas12aV2Bio ProtocJune 20, 2026Teng Hu, Youyang Pei, Zhaoyi Hu, Jing Feng, Qiangyuan Jiang, Li Hu, Yi LiuPCR buffer (with Mg2+) (Beyotime, catalog number: D7223-1) 2. 10× NEBuffer r2.1 (NEB, catalog number: B6002S) 3. 1 M Tris pH 8.0, sterile (Sangon Biotech, catalog number: B548127-0500) 4. Sodium chloride (NaCl)

(4) Homogeneous Antibody–DNA Conjugates Using Unmodified Oligonucleotides and Photo-Cross-Linkable Protein G-HUH Endonuclease Fusion ProteinsBioconjugate ChemistryJune 17, 2026Anna Swietlikowska, Femi Hesen, Alexander Gräwe, Maarten MerkxpM antibody–DNA conjugates, 40 μM dNTPs, 0.016 mg/mL salmon sperm DNA, and 1x NEB Buffer r2.1 (NEB, #B6002S). The target was added, and the mixtures were incubated for 30 min at 37 °C. 0.5 μL amount of T4 polymerase

(5) Plant-associated fungi co-opt ancient antimicrobials for host manipulationSci AdvApril 29, 2026Fantin Mesny, Valentina Wolf, Ana López-Moral, Anton Kraege, Wilko Punt, Saifei Liu, Jinyi Zhu, Jiyeun Park, Yukiyo Sato, Bart P. H. J. Thommatriphosphates (10 mM each), 2 μl of NEBuffer r2.1 (10×, New England Biolabs, Ipswich, MA, USA; ref. B6002S), 0.5 μl of T4 DNA polymerase (New England Biolabs; ref. M0203S), and 5 μl nuclease-free water were

(6) Functional decoupling of crRNA enables customizable CRISPR diagnosticsNucleic Acids ResMarch 3, 2026Hyungbin Park, Jiyoung Yun, Kyuhan Lee, Jee Hyeon Kim, Ji-Ho Park, Yeo-Jin Park, Jun Hyeok Park, Hoyeon Lee, Min-Gon Kimoligonucleotides used in this study were synthesized by BIONEER (South Korea). The 10× NEBuffer r2.1 (cat. #B6002S) was obtained from New England Biolabs (USA). The MIRA DNA Basic Kit (cat. #MIRAD2401) and MIRA RNA

(7) Direct delivery of assay reagents to extracellular vesicles in liquid biopsies for biomarker analysisNature ProtocolsJanuary 30, 2026Bo Ning, Long Chen, Brady M. Youngquist, Christopher J. Lyon, Yun Su, Tony Hu(New England Biolabs, cat no. M0368L)NEBuffer 2.1 (New England Biolabs, cat. no. B7202 (previously B6002S))Zymo Quick-DNA/RNA Viral Kit (Zymo Research, cat. no. D7020)DNA/RNA Shield (Zymo Research, cat. no.

(8) N-terminal modification of Histone H3 inhibits H3K27M-mediated loss of H3K27 trimethylationbioRxivJanuary 16, 2026Nicolas Poux, Daren F Zhang, Adam F Kebede, Isabella Gainey, Olohireme E Famous, Rameen Beroukhim, Pratiti Bandopadhayay(New England BioLabs, R0197S), 1 μL EcoRV-HF (New England BioLabs, R3195S), 5 μL 1X NEBuffer (New England BioLabs, B6002S), 1 μg DNA, and ddH2O up to a total reaction mixture of 50 μL. Digestion proceeded for 2 hours at room

(9) Nuclear cell-free DNA on the loose: an early warning signal of ischemia–reperfusion injury in kidney transplantationFrontiers in ImmunologyJanuary 7, 2026Gabriel Strandberg, Rebecca Trattner, Myriam Martin, Carl M. Öberg, Carl Raihle, Oleg Slivca, Shahnawaz Alam, Mårten Segelmark, Anders Christensson, Bo Nilsson, et al.(# R0104S; New England Biolabs, Ipswich, Massachusetts, United States) mixed with NE buffer r2.1 (#B6002S; New England Biolabs, Ipswich, Massachusetts, United States) and Supermix for probes (#L002699C; Bio-Rad,

(10) Amplification-free cancer diagnosis based on inhibition of Cas12a activity by site-specific 5mC-modified cfDNANucleic Acids ResDecember 11, 2025Feng Yang, Can Xu, Chunhong Li, Xinni Xiang, Yi Zhao, Cong Hu, Huan Rong, Yu He, Jingyu Li, Yu Wang, et al.BioLabs Inc. (M0653T, NEB, USA). 10× NEBuffer r2.1 buffer was obtained from New England BioLabs Inc. (B6002S, NEB, USA). AsCas12a was procured from Genscript (Z03502, Genscript, China), and FnCas12a was purchased

(11) Pathways and products of base excision DNA repair in Xenopus laevis eggs: contrast with human cell pathwaysNucleic Acids ResDecember 10, 2025Zhouyiyuan Xue, Patrick James Sutton, John D Haley, Christopher W Brownlee, Bruce Dempleof HindIII and AflIII, 10 μg of previously purified plasmid was linearized in NEB buffer r2.1 (NEB, B6002S) in a total reaction volume of 50 μl. Following 1 h of incubation at 37°C, heat inactivation of the

(12) Argonaute-HNH filaments triggered by invader DNA confer bacterial immunityNature CommunicationsNovember 28, 2025Anna Kanevskaya, Manju Narwal, Lidiya Lisitskaya, Andrey V. Moiseenko, Olga S. Sokolova, Nikolai N. Sluchanko, Katsuhiko S. Murakami, Andrey Kulbachinskiyreaction, the DNA samples were supplemented with 15 μl of MiliQ-grade water, 5 μl of 10x NEBuffer 2.1 (B6002S), 2.5 μl of 10 mM dATP and 2.5 μl of 5 U/μl Klenow (exo-) (NEB). The reactions were carried out for

(13) A specific, stable, and accessible LAMP assay targeting the HSP70 gene of Trypanosoma cruziMicrobiology SpectrumNovember 4, 2025Sneider Alexander Gutierrez Guarnizo, Beth Jessy Condori, Luciana Basma, Shirley Equilia, Edith Malaga, Siena Defazio, Emily Arteaga, Jean Karla Velarde, Martín Obregón, Anshule Takyar, et al.of the vial, 20 µL of the Cas12a reaction mix was added, which included 2× NEB Buffer r2.1 (NEB, CN: B6002S), 8 U RNAase Inhibitor (Thermo Fisher, CN: N8080119), and 0.125 µM gRNA (5′- /AlTR1/rUrArArUrUrUrCrUrArCrUrArArGrUrGrUrArGrArUrCrGrUrCrArArUrGrCrGrCrUrCrGrCrGrCrUrGrU/AlTR2/−3′),

(14) An inducible model of human post-implantation development derived from primed and naive stem cellsCell stem cellOctober 2, 2025Oura S, Li L, Wu J.,NEW ENGLAND BioLabs,E2611 BamHI,NEW ENGLAND BioLabs,R0136S HindIII,NEW ENGLAND BioLabs,R0104S NEBuffer r2.1,NEW ENGLAND BioLabs,B6002S NEBuffer r3.1,NEW ENGLAND BioLabs,B6003S PrimeSTAR GXL DNA Polymerase,Takara Bio,R050B Lipofectamin

(15) Simultaneous Capture of Chromatin-Associated RNA and Global RNA–RNA Interactions With Reduced Input RequirementsBio ProtocSeptember 5, 2025Cheng Ding, Guoting Chen, Shiping Luan, Yuanyuan Gong, Cuilin Gui, Chen Yang, Zihe Xiang, Junjie Du, Mohamed F. Foda, Jiapei Yan, et al.DNA polymerase (3 U/μL) (NEB, catalog number: M0203L) 33. NEBufferTM r2.1 (10×) (NEB, catalog number: B6002S) 34. Tween 20 (Sigma-Aldrich, catalog number: P9416-100ml) 35. Isopropanol (Sigma-Aldrich, catalog number:

(16) Simultaneous Capture of Chromatin-Associated RNA and Global RNA–RNA Interactions With Reduced Input RequirementsBIO-PROTOCOLSeptember 5, 2025Cheng Ding, Guoting Chen, Shiping Luan, Yuanyuan Gong, Cuilin Gui, Chen Yang, Zihe Xiang, Junjie Du, Mohamed F. Foda, Jiapei Yan, et al.DNA polymerase (3 U/μL) (NEB, catalog number: M0203L) 33. NEBufferTM r2.1 (10×) (NEB, catalog number: B6002S) 34. Tween 20 (Sigma-Aldrich, catalog number: P9416-100ml) 35. Isopropanol (Sigma-Aldrich, catalog number:

(17) Translocations can drive expression changes of multiple genes in regulons covering entire chromosome armsNucleic Acids ResAugust 22, 2025Anna Oncins, Roser Zaurin, Houyem Toukabri, Kimberly Quililan, José R Hernández Mora, Magdalena A Karpinska, Erik Wernersson, Alastair Smith, Agostina Bianchi, Leone Albinati, et al.Tris–HCl pH 7.5 and stored at -20°C. Cells were then resuspended in 1X NEBuffer r2.1 (NEB; cat. no. B6002S) and digested overnight with 100 U Csp6I (NEB; 10 000 U/mL; #R0639S) at 25°C, 900 RPM, prior to PCI/ethanol

(18) A naked-eye biosensing system based on one-pot RPA-CRISPR/Cas12a driver G4-hemin self-assembly for Mycobacterium tuberculosisFront ChemAugust 7, 2025Ting Yuan, Jianbo Yuan, Jian Huang, Nana Li, Huan Cai, Yan Yang, Juan Li, Rui Chen, Xun MinMycoplasma pneumoniae, were used for specificity tests. LbaCas12a (Cpf1, M0653T) and NEBuffer 2.1 (B6002S) were purchased from New England Biolabs (United States). The lyophilized TwistAmp Basic kit (product

(19) TAC-C uncovers open chromatin interaction in crops and SPL-mediated photosynthesis regulationSci AdvMay 30, 2025Jingmin Kang, Zhaoheng Zhang, Xuelei Lin, Fuyan Liu, Yali Song, Peng Zhao, Yujing Lin, Xumei Luo, Xiaoyi Li, Yanyan Yang, et al.supernatant, the pellets were washed twice with NIB and then washed twice with 1.2× NEBuffer r2.1 (NEB, B6002S). Nuclei were obtained after centrifugation at 1260g at 4°C for 5 min with the removal of the supernatant.

(20) Single-cell parallel analysis of DNA damage and transcriptome reveals selective genome vulnerabilityNature MethodsMay 1, 2025Dongsheng Bai, Zhenkun Cao, Nivedita Attada, Jinghui Song, Chenxu Zhuunder the same conditions for 10 mins. The nuclei were resuspended in 800 μL 1× NEBuffer r2.1 (NEB, B6002S) with 0.3% SDS and incubated at 42°C, 300 r.p.m. for 30 mins using a Thermomixer (Eppendorf). After

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