NEBuffer 2.R Buffer from New England Biolabs

Product Specs
ItemNEBuffer 2.R Buffer
CompanyNew England Biolabs
Price $27.00 Inquire
Catalog NumberB6002S
Temperature-20 C
ApplicationsDNA Manipulation
TypeBuffer
Quantity5 ml

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Plant-associated fungi co-opt ancient antimicrobials for host manipulationSci AdvApril 29, 2026Fantin Mesny, Valentina Wolf, Ana López-Moral, Anton Kraege, Wilko Punt, Saifei Liu, Jinyi Zhu, Jiyeun Park, Yukiyo Sato, Bart P. H. J. Thommatriphosphates (10 mM each), 2 μl of NEBuffer r2.1 (10×, New England Biolabs, Ipswich, MA, USA; ref. B6002S), 0.5 μl of T4 DNA polymerase (New England Biolabs; ref. M0203S), and 5 μl nuclease-free water were

(2) Functional decoupling of crRNA enables customizable CRISPR diagnosticsNucleic Acids ResMarch 3, 2026Hyungbin Park, Jiyoung Yun, Kyuhan Lee, Jee Hyeon Kim, Ji-Ho Park, Yeo-Jin Park, Jun Hyeok Park, Hoyeon Lee, Min-Gon Kimoligonucleotides used in this study were synthesized by BIONEER (South Korea). The 10× NEBuffer r2.1 (cat. #B6002S) was obtained from New England Biolabs (USA). The MIRA DNA Basic Kit (cat. #MIRAD2401) and MIRA RNA

(3) Nuclear cell-free DNA on the loose: an early warning signal of ischemia–reperfusion injury in kidney transplantationFrontiers in ImmunologyJanuary 7, 2026Gabriel Strandberg, Rebecca Trattner, Myriam Martin, Carl M. Öberg, Carl Raihle, Oleg Slivca, Shahnawaz Alam, Mårten Segelmark, Anders Christensson, Bo Nilsson, et al.(# R0104S; New England Biolabs, Ipswich, Massachusetts, United States) mixed with NE buffer r2.1 (#B6002S; New England Biolabs, Ipswich, Massachusetts, United States) and Supermix for probes (#L002699C; Bio-Rad,

(4) Amplification-free cancer diagnosis based on inhibition of Cas12a activity by site-specific 5mC-modified cfDNANucleic Acids ResDecember 11, 2025Feng Yang, Can Xu, Chunhong Li, Xinni Xiang, Yi Zhao, Cong Hu, Huan Rong, Yu He, Jingyu Li, Yu Wang, et al.BioLabs Inc. (M0653T, NEB, USA). 10× NEBuffer r2.1 buffer was obtained from New England BioLabs Inc. (B6002S, NEB, USA). AsCas12a was procured from Genscript (Z03502, Genscript, China), and FnCas12a was purchased

(5) Pathways and products of base excision DNA repair in Xenopus laevis eggs: contrast with human cell pathwaysNucleic Acids ResDecember 10, 2025Zhouyiyuan Xue, Patrick James Sutton, John D Haley, Christopher W Brownlee, Bruce Dempleof HindIII and AflIII, 10 μg of previously purified plasmid was linearized in NEB buffer r2.1 (NEB, B6002S) in a total reaction volume of 50 μl. Following 1 h of incubation at 37°C, heat inactivation of the

(6) Argonaute-HNH filaments triggered by invader DNA confer bacterial immunityNature CommunicationsNovember 28, 2025Anna Kanevskaya, Manju Narwal, Lidiya Lisitskaya, Andrey V. Moiseenko, Olga S. Sokolova, Nikolai N. Sluchanko, Katsuhiko S. Murakami, Andrey Kulbachinskiyreaction, the DNA samples were supplemented with 15 μl of MiliQ-grade water, 5 μl of 10x NEBuffer 2.1 (B6002S), 2.5 μl of 10 mM dATP and 2.5 μl of 5 U/μl Klenow (exo-) (NEB). The reactions were carried out for

(7) A specific, stable, and accessible LAMP assay targeting the HSP70 gene of Trypanosoma cruziMicrobiology SpectrumNovember 4, 2025Sneider Alexander Gutierrez Guarnizo, Beth Jessy Condori, Luciana Basma, Shirley Equilia, Edith Malaga, Siena Defazio, Emily Arteaga, Jean Karla Velarde, Martín Obregón, Anshule Takyar, et al.of the vial, 20 µL of the Cas12a reaction mix was added, which included 2× NEB Buffer r2.1 (NEB, CN: B6002S), 8 U RNAase Inhibitor (Thermo Fisher, CN: N8080119), and 0.125 µM gRNA (5′- /AlTR1/rUrArArUrUrUrCrUrArCrUrArArGrUrGrUrArGrArUrCrGrUrCrArArUrGrCrGrCrUrCrGrCrGrCrUrGrU/AlTR2/−3′),

(8) Simultaneous Capture of Chromatin-Associated RNA and Global RNA–RNA Interactions With Reduced Input RequirementsBIO-PROTOCOLSeptember 5, 2025Cheng Ding, Guoting Chen, Shiping Luan, Yuanyuan Gong, Cuilin Gui, Chen Yang, Zihe Xiang, Junjie Du, Mohamed F. Foda, Jiapei Yan, et al.DNA polymerase (3 U/μL) (NEB, catalog number: M0203L) 33. NEBufferTM r2.1 (10×) (NEB, catalog number: B6002S) 34. Tween 20 (Sigma-Aldrich, catalog number: P9416-100ml) 35. Isopropanol (Sigma-Aldrich, catalog number:

(9) Translocations can drive expression changes of multiple genes in regulons covering entire chromosome armsNucleic Acids ResAugust 22, 2025Anna Oncins, Roser Zaurin, Houyem Toukabri, Kimberly Quililan, José R Hernández Mora, Magdalena A Karpinska, Erik Wernersson, Alastair Smith, Agostina Bianchi, Leone Albinati, et al.Tris–HCl pH 7.5 and stored at -20°C. Cells were then resuspended in 1X NEBuffer r2.1 (NEB; cat. no. B6002S) and digested overnight with 100 U Csp6I (NEB; 10 000 U/mL; #R0639S) at 25°C, 900 RPM, prior to PCI/ethanol

(10) A naked-eye biosensing system based on one-pot RPA-CRISPR/Cas12a driver G4-hemin self-assembly for Mycobacterium tuberculosisFront ChemAugust 7, 2025Ting Yuan, Jianbo Yuan, Jian Huang, Nana Li, Huan Cai, Yan Yang, Juan Li, Rui Chen, Xun MinMycoplasma pneumoniae, were used for specificity tests. LbaCas12a (Cpf1, M0653T) and NEBuffer 2.1 (B6002S) were purchased from New England Biolabs (United States). The lyophilized TwistAmp Basic kit (product

(11) TAC-C uncovers open chromatin interaction in crops and SPL-mediated photosynthesis regulationSci AdvMay 30, 2025Jingmin Kang, Zhaoheng Zhang, Xuelei Lin, Fuyan Liu, Yali Song, Peng Zhao, Yujing Lin, Xumei Luo, Xiaoyi Li, Yanyan Yang, et al.supernatant, the pellets were washed twice with NIB and then washed twice with 1.2× NEBuffer r2.1 (NEB, B6002S). Nuclei were obtained after centrifugation at 1260g at 4°C for 5 min with the removal of the supernatant.

(12) Single-cell parallel analysis of DNA damage and transcriptome reveals selective genome vulnerabilityNature MethodsMay 1, 2025Dongsheng Bai, Zhenkun Cao, Nivedita Attada, Jinghui Song, Chenxu Zhuunder the same conditions for 10 mins. The nuclei were resuspended in 800 μL 1× NEBuffer r2.1 (NEB, B6002S) with 0.3% SDS and incubated at 42°C, 300 r.p.m. for 30 mins using a Thermomixer (Eppendorf). After

(13) Cell differentiation controls iron assimilation in the choanoflagellate Salpingoeca rosettamSphereMarch 25, 2025Fredrick Leon, Jesus M. Espinoza-Esparza, Vicki Deng, Maxwell C. Coyle, Sarah Espinoza, David S. Boothmastermix was assembled in two parts. First, Cas12a RNP (13 µL water; 2 µL r2.1 buffer [NEB, Cat. No. B6002S]; 2.5 µL 100 µM gRNA [ordered as a synthesized oligo]; 2 µL 100 µM LbaCas12a [NEB, Cat. No. M0653T])

(14) Rapid and robust generation of cardiomyocyte-specific crispants in zebrafish using the cardiodeleter systemCell Reports MethodsMarch 24, 2025Sean Keeley, Miriam Fernández-Lajarín, David Bergemann, Nicolette John, Lily Parrott, Brittany E. Andrea, Juan Manuel González-RosaB0202S rCutSmart™ Buffer,New England Biolabs,Cat# B6004S NEBuffer™ r2.1,New England Biolabs,Cat# B6002S

(15) Amplification-free, OR-gated CRISPR-Cascade reaction for pathogen detection in blood samplesProceedings of the National Academy of SciencesMarch 18, 2025Jongwon Lim, An Bao Van, Katherine Koprowski, Matthew Wester, Enrique Valera, Rashid Bashir(M0314L) was acquired from NEB. The DNA reporter probe was synthesized by IDT. NEBuffer™ r2.1 (5 mL, B6002S) was purchased from NEB. Human Genomic DNA (100 µg, G3041) was obtained from Promega. DNA and Pathogens. MRSA

(16) A specific, stable, and accessible LAMP assay targeting the HSP70 gene of Trypanosoma cruzimedRxivJanuary 13, 2025Sneider Alexander Gutierrez Guarnizo, Luciana Basma, Shirley Equilia, Beth Jessy Condori, Edith Malaga, Siena Defazio, Emily Arteaga, Jean Karla Velarde, Martín Obregón, Anshule Takyar, et al.the vial, 20 μL of the Cas12a reaction mix were added, which included: 2X NEB Buffer r2.1 (NEB, CN: B6002S), 8 U RNAase Inhibitor (ThermoFisher, CN: N8080119), 0.125 μM gRNA (5’-/AlTR1/rUrArArUrUrUrCrUrArCrUrArArGrUrGrUrArGrArUrCrGrUrCrArArUrGrCrGrCrUrCrGrCrGrCrUrGrU/AlTR2/−3’),

(17) Protocol for developing Pseudomonas aeruginosa type III secretion system-neutralizing monoclonal antibodies from human B cellsSTAR ProtocolsDecember 20, 2024Alexandra Albus, Christoph Kreer, Florian Klein, Jan Rybniker, Alexander Simonis,Thermo Fisher Scientific,Cat.# 10966034 Moxifloxacin-hydrochloride,Sigma-Aldrich,Cat.# SML1581 NEB 2.1 buffer,NEB,Cat.#B6002S NP-40,Thermo Fisher Scientific,Cat.# 85124 Nuclease-free water,Thermo Fisher Scientific,Cat.# AM9937 Phusion

(18) Protocol for CRISPR-based endogenous protein tagging in mammalian cellsSTAR ProtocolsDecember 20, 2024Yu-Xi Xiao, Jiarun Wei, Jason Moffatbuffer,NEB,B0202S rSAP,NEB,M0371S EcoRI-HF,NEB,R3101S HindIII-HF,NEB,R3104S 10x NEB2.1 buffer,NEB,B6002S BbsI,NEB,R0539S MyTaq HS red mix,Bioline,BIO-25047 Alt-R L.b. Cas12a (Cpf1) Ultra,IDT,10007922 Alt-R L.b. Cas12a crRNA

(19) Efficient CRISPR/Cas9 Knock‐in Approaches for Manipulation of Endogenous Genes in Human B Lymphoma CellsCurrent ProtocolsNovember 13, 2024Laura A. Murray‐Nerger, Benjamin E. Gewurzvector. Materials: eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA (Addgene #86613) BbsI (NEB R3539) NEB Buffer 2.1 (NEB B6002S) Sterile water (autoclaved, or purchased as RNAse and DNAse free) Agarose 1x TAE Buffer (see recipe

(20) CRISPR/Cas-mediated “one to more” lighting-up nucleic acid detection using aggregation-induced emission luminogensNature CommunicationsOctober 3, 2024Yuqian Guo, Yaofeng Zhou, Hong Duan, Derong Xu, Min Wei, Yuhao Wu, Ying Xiong, Xirui Chen, Siyuan Wang, Daofeng Liu, et al.Thermo Fisher Scientific Inc. (Waltham, MA). EnGen LbaCas12a (Cpf1, cat. no. M0653T) and NEBuffer 2.1 (cat. no. B6002S) were obtained from New England BioLabs (Ipswich, MA). The lyophilized TwistAmp® Basic kit (product

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