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Protein Biochemistry
Chemicals and Reagents
Cal-520®, AM
Cal-520®, AM from AAT Bioquest, Inc.
Be the first to write a review!
Citations:
(7)
Supplier Page
Supplier Page from
AAT Bioquest, Inc. for
Cal-520®, AM
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Description
Cal-520® AM provides a robust homogeneous fluorescence-based assay tool for detecting intracellular calcium mobilization
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Cal-520®, AM
Company
AAT Bioquest, Inc.
Price
$334.00
Supplier Page
View Company Product Page
Catalog Number
21131
Quantity
1 mg
Add to Compare List
AAT Bioquest, Inc.
520 Mercury Drive
Sunnyvale
,
CA
94085
United States
Phone
: 1-800-990-8053 (US and Canada)
1-408-733-1055 (International)
Email: info@aatbio.com
Company Profile
Website:
https://www.aatbio.com/
Citations (7)
(1)
A Hypoxia-reoxygenation Injury Model in Self-assembling Human Cardioids
Journal of Visualized Experiments
March 17, 2026
Lauren E. Parker, Michael C. Thomas, Ashley B. Williams, Rachel Weirnick, Alícia S. Ogliari, Anish Karpurapu, Claudia Barreto, Sneha Manepalli, Katelyn C. Peña, Alena N. Brandt,
et al.
"
B-27™ Supplement (50X), serum free
",
Gibco
,
17504044
"
B-27™ Supplement, minus insulin
",
Gibco
,
A1895602
"
Cal-520®, AM
",
AAT Bioquest
,
21131
"Charged Microscope Slides, White Glass",VWR,89500-496
CHIR 99021
,
Tocris
,4423
CloneR
™,
STEMCELL Technologies
,5888 Corning™
(2)
Regional Differences in Calcium Dynamics in the Intact Lens: The Role of TRPV Channels in Lens Calcium Regulation
Invest Ophthalmol Vis Sci
December 11, 2025
Yadi Chen, Kevin F. Webb, Paul J. Donaldson
selectively image Ca²⁺ in
epithelial cells
, dissected lenses were incubated in 3 µM Cal520 AM (Cat no.
21131
;
AAT Bioquest
, Sunnyvale, CA, USA) for 40 minutes at 33°C in AAH. This allowed passive diffusion of
(3)
Serine synthesis pathway regulates cardiac differentiation from human pluripotent stem cells
iScience
July 18, 2025
Tomohiko C. Umei, Shugo Tohyama, Yuika Morita-Umei, Manami Katoh, Seitaro Nomura, Kotaro Haga, Takako Hishiki, Tomomi Matsuura, Hidenori Tani, Yusuke Soma,
et al.
previous report.76 Briefly, on the day of the
assay
,
hiPSC
-CMs were incubated in 5 mM
Cal-520®, AM
(
21131
,
AAT Bioquest
) in Hanks’ balanced salt solution (
14025092
,
Thermo Fisher Scientific
) supplemented with
(4)
Modulation of Ca2+ oscillation following ischemia and nicotinic acetylcholine receptors in primary cortical neurons by high-throughput analysis
Scientific Reports
November 12, 2024
Tsutomu Sasaki, Sunao Hisada, Hideaki Kanki, Kazuto Nunomura, Bangzhong Lin, Kumiko Nishiyama, Tomohito Kawano, Shigenobu Matsumura, Hideki Mochizuki
neurons35. The EarlyTox Cardiotoxicity Kit utilizes a calcium-sensitive indicator equivalent to Cal520AM (#
21131
,
AAT Bioquest
, Sunnyvale, CA, USA) to detect intracellular Ca2+ concentrations. Indeed, Ca2+ oscillations
(5)
High-Throughput Screening Assay for Detecting Drug-Induced Changes in Synchronized Neuronal Oscillations and Potential Seizure Risk Based on Ca2+ Fluorescence Measurements in Human Induced Pluripotent Stem Cell (hiPSC)-Derived Neuronal 2D and 3D Cultures
Cells
March 21, 2023
Hua-Rong Lu, Manabu Seo, Mohamed Kreir, Tetsuya Tanaka, Rie Yamoto, Cristina Altrocchi, Karel van Ammel, Fetene Tekle, Ly Pham, Xiang Yao,
et al.
3D Cells maintained under 2D conditions were incubated with 2 μM calcium-indicating dye, Cal-520 (#
21131
,
AAT Bioquest
, Sunnyvale, CA, USA), at 37 °C for 2 h as instructed by the manufacturer. After the
incubation
,
(6)
Identifying Acute Cardiac Hazard in Early Drug Discovery Using a Calcium Transient High-Throughput Assay in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Frontiers in Physiology
April 25, 2022
Hua Rong Lu, Mohamed Kreir, Van Ammel Karel, Fetene Tekle, Danny Geyskens, Ard Teisman, David J. Gallacher
to yield isokalemic (4.2 mM K+) conditions. The Ca2+-sensitive
fluorescence
dye
Cal-520™ AM
(Cat. no.
21131
;
AAT Bioquest
) was used to capture the intracellular Ca2+ transients in
hiPSC
-CMs. The protocol used
(7)
A Thalamic Orphan Receptor Drives Variability in Short-Term Memory
Cell
October 15, 2020
Hsiao K, Noble C, Pitman W, Yadav N, Kumar S, Keele GR, Terceros A, Kanke M, Conniff T, Cheleuitte-Nieves C,
et al
.
1199236-64-0
L-glutamic acid
,
Sigma-Aldrich
,RES5063G-A701X; CAS#6106-04-3 Cal520-AM dye,
Aat Bioquest
,
21131
Critical Commercial
Assays
,Critical Commercial
Assays
,Critical Commercial
Assays
Total RNA Purification
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Cal-520®, AM from AAT Bioquest, Inc.
Supplier Page
View Company Product Page
$334.00
Product Specs
Company Info
Citations
Reviews
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