Calbryte™ 520 AM from AAT Bioquest, Inc.

Product Specs
ItemCalbryte™ 520 AM
CompanyAAT Bioquest, Inc.
Price $334.00Supplier Page View Company Product Page
Catalog Number20651
Quantity10x50 ug

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AAT Bioquest, Inc.

520 Mercury Drive

Sunnyvale, CA 94085

United States

Phone: 1-800-990-8053 (US and Canada)
1-408-733-1055 (International)

Email: info@aatbio.com

Company Profile

Website: https://www.aatbio.com/

(1) Kinemomics: spatiotemporal morphodynamic mapping of ventricular kinematic subpopulations in organotypic fetal heart slicesbioRxivMay 25, 2026Gening Dong, Linyang Wu, Junho Choe, Dylan Mostert, Mingkun Wang, Jonathan T. ButcherAnimal Care and Use Committee (IACUC). Calcium imaging and analysis Calbryte 520 AM (AAT Bioquest, 20651) was used as the fluorescent calcium indicator. Slices were incubated with 10 μM Calbryte 520 AM in

(2) Biosensor Cell Array Reveals Temporal GABA Secretion Dynamics from Pancreatic IsletsbioRxivApril 3, 2026Austin E. Stis, Charles S. Lazimi, Sandra M. Ferreira, Alexandra E. Cuaycal, Dylan Smurlick, D. Walker Hagan, Taylor Nakayama, Sunil P. Gandhi, Emery Smith, Timothy P. Spicer, et al.resuspended in KRBH with 5 mM glucose (5G). Biosensor cells were loaded with Calbryte 520 AM (AAT Bioquest 20651) for 30 minutes at 37°C. Islets were loaded with Calbryte 590 AM (AAT Bioquest 20701), or Calbryte 520 AM

(3) Tri-Culture System Reveals an Activation Cascade From Microglia Through Astrocytes to Neurons During NeuroinflammationJ NeurochemMarch 20, 2026Hayato Kobayashi, Hiroshi Kato, Mitsuho Taniguchi, Setsu Endoh-Yamagamiculture medium and 50 μL of working solution containing 8 μM Calbryte 520 AM (AAT Bioquest, cat. no. 20651), 2.5 mM calcium chloride dihydrate (Fujifilm Wako, cat. no. 036‐19 731), 0.08% pluronic F‐127 (Thermo Fisher Scientific

(4) Human iPSC-derived salivary gland organoids model diabetic salivary gland dysfunctionbioRxiv : the preprint server for biologyOctober 3, 2025Ehnes DD, Morishita A, Phal A, Jung HY, Lim YC, Foreman Z, Cirulli V, Mathieu J, Ruohola-Baker H.low-glucose media containing 5mM Calbryte 520 AM fluorescent intracellular calcium indicator (AAT Bioquest, #20651) for 30 min at 37°C, then washed 3X with media. Just prior to image acquisition, cells were treated

(5) Assessing the potential of bacterial signal peptides for radiopharmaceutical applicationsScientific ReportsSeptember 12, 2025Zukaa Al Taleb, Ina Hierlmeier, Heiko Heilmann, Martin Jung, Mark Bartholomä, Bernd Bufewith a loading solution consisting of 2 µM Calbryte dye in C1 buffer (Calbryte 520AM, AAT Bioquest 20651) 50 µL per well, for at least 2 h at RT in the dark. After 2 h of loading with the fluorescent dye,

(6) Multiscale and recursive unmixing of spatiotemporal rhythms for live-cell and intravital cardiac microscopyNature Cardiovascular ResearchMay 7, 2025Zhi Ling, Wenhao Liu, Kyungduck Yoon, Jessica Hou, Parvin Forghani, Xuanwen Hua, Hansol Yoon, Maryam Bagheri, Lakshmi P. Dasi, Biagio Mandracchia, et al.Thermo Fisher Scientific) into one vial of Calbryte 520 AM (Fig. 2 and Extended Data Figs. 4 and 5; 20651, AAT Bioquest) or one vial of Fluo-4 AM (4, F14201, Thermo Fisher Scientific) and diluting it into 4.56

(7) Modulation of FGF pathway signaling and vascular differentiation using designed oligomeric assembliesCellJuly 11, 2024Natasha I. Edman, Ashish Phal, Rachel L. Redler, Thomas Schlichthaerle, Sanjay R. Srivatsan, Devon Duron Ehnes, Ali Etemadi, Seong J. An, Andrew Favor, Zhe Li, et al.serum-free media containing 5μM Calbryte 520 AM fluorescent intracellular calcium indicator [AAT Bioquest, #20651] for 30 min at 37°C. Cells were washed 3X with serum-free media and treated with various concentrations

(8) The endoplasmic reticulum plays a key role in α-cell intracellular Ca2+ dynamics and glucose-regulated glucagon secretion in mouse isletsiScienceMay 17, 2024Samuel Acreman, Jinfang Ma, Geoffrey Denwood, Rui Gao, Andrei Tarasov, Patrik Rorsman, Quan Zhangpentapotassium,ThermoFisher,Cat# F14200 cyclopiazonic acid,Sigma-Aldrich,Cat# C1530 Calbryte 520-AM,AAT Bioquest,Cat# 20651 Calbryte 630-AM,AAT Bioquest,Cat# 20721 Isradipine,Alamone,Cat# I-100 Ryanodine,Bio-techne,Cat# 1329/1 ω-agatoxin

(9) Piezo1 activates noncanonical EGFR endocytosis and signalingScience advancesSeptember 29, 2023Pardo-Pastor C, Rosenblatt J.on glass coverslips were loaded with 4.5 μM of the Ca2+-sensitive dye Calbryte 520 AM (AAT Bioquest, 20651, reconstituted in DMSO) for 30 min in the cell culture incubator (37°C, 5% CO2), followed by washing

(10) Protocol to generate and utilize pancreatic tissue slices to study endocrine and exocrine physiology in situ from mouse and human tissueSTAR ProtocolsSeptember 1, 2023Julia K. Panzer, Alejandro CaicedoA1153 Arginine,Sigma,Cat# A5006 Bovine berum albumin,Sigma,Cat# 9048-46-8 Calbryte 520 AM,AAT Bioquest,Cat# 20651 Calcium chloride,Sigma,Cat# C4901 Epinephrine,Sigma,Cat# E4375 Fluo4-AM,Invitrogen,Cat# F14201 Glutamic

(11) An airway-to-brain sensory pathway mediates influenza-induced sicknessNatureMarch 1, 2023Bin NR, Prescott SL, Horio N, Wang Y, Chiu IM, Liberles SD.(3 min, 300g, 4 °C). Cells were then resuspended in culture medium containing 5 μM Calbryte 520 AM (20651, AAT Bioquest); culture medium contains 1× B27 (Gibco, 17504044), 1× N2 (Gibco, 17502048), 1× penicillin

(12) Downregulated Calcium-Binding Protein S100A16 and HSP27 in Placenta-Derived Multipotent Cells Induce Functional Astrocyte DifferentiationStem Cell Reviews and ReportsFebruary 1, 2022Yu-Che Cheng, Chi-Jung Huang, Wei-Chi Ku, Shu-Lin Guo, Lu-Tai Tien, Yih-Jing Lee, Chih-Cheng Chiencells were first cultured in a 96-well microplate and incubated with 2 μM Calbryte 520 calcium dye (#20651, AAT Bioquest, CA, USA) in phenol red-free DMEM (#30153028, Gibco/Thermo Fisher) for 1 h at 37 °C. The

(13) Influence of Acetylcholine Esterase Inhibitors and Memantine, Clinically Approved for Alzheimer’s Dementia Treatment, on Intestinal Properties of the MouseInternational Journal of Molecular SciencesJanuary 20, 2021Vu Thu Thuy Nguyen, Jason Sallbach, Malena dos Santos Guilherme, Kristina EndresHitech Expert 96 UV Microplate Reader). 4.12. Calcium Influx Measurement Calbryte 520-AM (AAT Bioquest 20651, Sunnyvale, CA, USA) was used to measure the calcium influx upon KCl administration. 62.5 μL supernatant

(14) Differentiation of Human iPS Cells Into Sensory Neurons Exhibits Developmental Stage-Specific Cryopreservation ChallengesFrontiers in cell and developmental biologyJanuary 1, 0001"Li R, Walsh P, Truong V, Petersen A, Dutton JR, Hubel A."camera. Calcium Imaging Live D14 mSN cultures were loaded with 2.5 µM Calbryte 520 AM (AAT Bioquest, 20651) and PowerLoad Concentrate (1:100, ThermoFisher Scientific, P10020) for 1 h before imaging. Fluorescent

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Calbryte™ 520 AM from AAT Bioquest, Inc.

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AAT Bioquest, Inc.

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Calbryte™ 520 AM from AAT Bioquest, Inc.

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