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Protein Biochemistry
Chemicals and Reagents
Calcein, AM
Calcein, AM from AAT Bioquest, Inc.
Be the first to write a review!
Citations:
(9)
Supplier Page
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AAT Bioquest, Inc. for
Calcein, AM
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Description
Calcein AM readily passes through the cell membrane of viable cells because of its enhanced hydrophobicity as compared to calcein
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Calcein, AM
Company
AAT Bioquest, Inc.
Price
$109.00
Supplier Page
View Company Product Page
Catalog Number
22002
Quantity
1 mg
Format
CAS 148504-34-1
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AAT Bioquest, Inc.
520 Mercury Drive
Sunnyvale
,
CA
94085
United States
Phone
: 1-800-990-8053 (US and Canada)
1-408-733-1055 (International)
Email: info@aatbio.com
Company Profile
Website:
https://www.aatbio.com/
Citations (9)
(1)
Lipidomic changes in persister cancer cells drive enhanced ferroptosis sensitivity
Ferroptosis and Oxidative Stress
December 29, 2025
Eduard Reznik, Fereshteh Zandkarimi, Joleen M. Csuka, Qiulin Zhu, Jenny Jin, Taruna Vani Neelakantan, Jiewen Zheng, Vasiliki Polychronidou, Mark Fongheiser, Ashley Brown,
et al.
HT1080, were collected, washed twice with PBS, resuspended in 500 nM of
calcein-AM
(
AAT Bioquest
, #
22002
) and incubated at 37 °C in the dark for 20 minutes. Cells were then centrifuged at 300 g for 5 minutes
(2)
Co-targeting of glial activation and inflammation by tsRNA-Gln-i-0095 for treating retinal ischemic pathologies
Cell Commun Signal
January 10, 2025
Ying Zhang, Yan Ma, Yu-Ke Ji, Yi-Fei Jiang, Duo Li, Wan Mu, Mu-Di Yao, Jin Yao, Biao Yan
fluorescent microscope
.
Calcein-AM
and propidium iodide (PI) double
staining
Calcein-AM
(
AAT Bioquest
,
22002
, USA) can penetrate live
cell membranes
and react with intracellular esterases to form calcein, which
(3)
Silicon-Rhodamine Functionalized Evocalcet Probes Potently and Selectively Label Calcium Sensing Receptors In Vitro, In Vivo, and Ex Vivo
ACS Pharmacology & Translational Science
May 10, 2024
Daniel Bátora, Jérôme P. Fischer, Reto M. Kaderli, Máté Varga, Martin Lochner, Jürg Gertsch
were evaluated using the
Calcein AM
assay
as described by the manufacturer’s protocol (
AAT Bioquest
,
22002
) with a working concentration of 10 μM. For the
assay
, cells were incubated in various concentrations
(4)
A small molecule inhibitor of leucine carboxyl methyltransferase-1 inhibits cancer cell survival
Front Drug Discov (Lausanne)
April 11, 2024
O. A. Arosarena, A. S. Saribas, E. P. Papadopoulos
catalog #D1283). Cells were stained with 3 μM
calcein-AM
(
AAT Bioquest
, Pleasanton, California, catalog #
22002
) in DPBS for 30 minutes in the dark at 37°C. Plates were immediately read with a Biotek fluorescent
(5)
Hyperglycemia-regulated tRNA-derived fragment tRF-3001a propels neurovascular dysfunction in diabetic mice
Cell Reports Medicine
October 1, 2023
Jun-Ya Zhu, Wen Yao, Xi-Sen Ni, Mu-Di Yao, Wen Bai, Tian-Jing Yang, Zi-Ran Zhang, Xiu-Miao Li, Qin Jiang, Biao Yan
PowerUp™ SYBR™ Green Master Mix
,
Thermo Fisher Scientific
,Cat#
A25742
CCK-8,Beyotime,Cat# C0038
Calcein-AM
,
AAT Bioquest
,Cat#
22002
PI,Biofroxx,Cat# 1246MG100 Rhodamine123,Solarbio,Cat#
R8030
Methyl cellulose
solution,
Sigma-Aldrich
,Cat#
(6)
Regulatory effect of long-stranded non-coding RNA-CRNDE on neurodegeneration during retinal ischemia-reperfusion
Heliyon
October 1, 2022
Sun TT, Li XM, Zhu JY, Yao W, Yang TJ, Meng XR, Yao J, Jiang Q.
and dead cells. Cells were stained with a mixture of 10 μmol/L
Calcein-AM
solutions (
AAT Bioquest
,
22002
), 10 μmol/L PI (Biofroxx, 1246MG100), and 3 μM Hoechst 33342 (Biofroxx, 2289MG025) for 15 min at 37
(7)
Suppression of choroidal neovascularization by silencing of long non-coding RNA IPW
Aging (Albany NY)
April 15, 2021
Tian-Jing Yang, Mu-Di Yao, Ya-Nan Sun, Xiu-Miao Li, Qin Jiang, Biao Yan
manufacturer's instruction. After the required treatment, RF/6A cells were stained with
Calcein-AM
(2 μg/mL;
22002
,
AAT Bioquest
, USA) and PI (1 μg/mL; 1246MG100, Biofroxx, Germany) at 37°C for 15 min. The
fluorescence
(8)
PARIS, an optogenetic method for functionally mapping gap junctions
eLife
January 14, 2019
Ling Wu, Ao Dong, Liting Dong, Shi-Qiang Wang, Yulong Li
Scientific Cat#415422 Chemical compound,drug Forskolin
TargetMol
Cat#
T2939
Chemical compound,drug 8-Br-cAMP
Sigma-Aldrich
Cat#
B7880
Chemical compound,drug TPA
Sigma-Aldrich
Cat#
P1585
Chemical compound,drug
Calcein-AM
AAT Bioquest
Cat#
22002
Chemical compound,drug
Fluo-8-AM
AAT Bioquest
Cat#
21082
Recombinant DNA reagent pcDNA3.1 vector Michael
(9)
PARIS, an optogenetic method for functionally mapping gap junctions
Elife
January 14, 2019
Ling Wu, Ao Dong, Liting Dong, Shi-Qiang Wang, Yulong Li
Scientific Cat#415422 Chemical compound,drug Forskolin
TargetMol
Cat#
T2939
Chemical compound,drug 8-Br-cAMP
Sigma-Aldrich
Cat#
B7880
Chemical compound,drug TPA
Sigma-Aldrich
Cat#
P1585
Chemical compound,drug
Calcein-AM
AAT Bioquest
Cat#
22002
Chemical compound,drug
Fluo-8-AM
AAT Bioquest
Cat#
21082
Recombinant DNA reagent pcDNA3.1 vector Michael
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Calcein, AM from AAT Bioquest, Inc.
Supplier Page
View Company Product Page
$109.00
Product Specs
Company Info
Citations
Reviews
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