Puromycin (10 mg/ml; 10 x 1 ml) from ScienCell Research Laboratories

Puromycin is an aminonucleoside antibiotic isolated from Streptomyces alboniger. Puromycin terminates protein synthesis at the level of translation in prokaryotic and eukaryotic cells and is active against gram-positive bacteria and weakly active against gram-negative bacteria. Resistance to puromycin is conferred by the puromycin N-acetyl-transferase (pac) gene [1, 2]. Exogenous expression of the pac gene is commonly used in conjunction with puromycin to generate stable cell lines. Most working concentrations range from 1-10 µg/ml; however, the optimal concentration should be determined for individual cell types and culture conditions. In most cases, puromycin kills 99% of non-resistant cells within 48 hours