Cycloheximide from Cell Signaling Technology

Product Specs
ItemCycloheximide
CompanyCell Signaling Technology
Price $144.00 Inquire
Catalog Number2112S
Quantity1 g

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Large-scale quaternary structural transitions underlie gain of function of SPOP cancer mutationsMol CellAugust 6, 2026Matthew J. Cuneo, Ömer Güllülü, Mohamed-Raafet Ammar, Xinrui Gui, Kelly Churion, Martin Turk, Brian G. O'Flynn, Nafiseh Sabri, Tanja Mittagproteins",, Ubiquitin,Boston Biochem,Cat# U-100H-10M MG132,Selleck Chemicals,Cat# S2619 Cycloheximide,Cell Signaling,Cat# 2112 Lipofectamine 3000,ThermoFisher,Cat# L3000008 F-BRD3 peptide: Ac-VKRKADTTTDTT,St. Jude Peptide Synthesis,N/A Deposited

(2) The titin N2A-MARP signalosome constrains muscle longitudinal hypertrophy in response to stretchElifeJuly 30, 2026Robbert van der Pijl, Jochen Gohlke, Joshua Strom, Eva Peters, Shengyi Shen, Stefan Conijn, Zaynab Hourani, Stephan Lange, Ju Chen, Paul Langlais, et al.(1:500) Antibody,anti-4EBP1(Rabbit IgG),Cell Signaling,Cat#9452,WB (1:750) Antibody,anti-GAPDH (Rabbit IgG),Cell Signaling,Cat#2112,WB (1:5000) Antibody,anti-GAPDH (Mouse IgG1),Thermo Fisher,Cat#MA5-15738,WB (1:3000) "Chemical compound,

(3) Silencing the Signal: The Metastasis Suppressor NDRG1 Disrupts Small Extracellular Vesicle‐Mediated Crosstalk in Pancreatic CancerJournal of Extracellular VesiclesJuly 1, 2026Jiawei Chang, Shafi Alenizi, Heloisa Zaccaron Milioli, Winston Lay, Saranya Pounraj, Yujie Li, Mekonnen Sisay Shiferaw, Elham Hosseini‐Beheshti, Zaklina KovacevicHalf‐life Assay MIAPaCa‐2 VC and NDRG1 cells were treated with cycloheximide (CHX, 15 µg/mL; CAT#: 2112, Cell Signalling) diluted in serum free media to inhibit protein synthesis. CHX treatment was administered

(4) FUCA2 Sustains AKT Signaling and Suppresses Senescence by Antagonizing FUT3‐Mediated ErbB3 Fucosylation in Lung AdenocarcinomaAdvanced ScienceJune 16, 2026Lu Chen, Jintao Guo, Fei Li, Mingjie Gao, Runyang Li, Zhaozhang Huang, Yaolin Zheng, Chunyi Gao, Jihuan Hou, Qiang Yu, et al.confluence. Following cell attachment, fresh medium containing cycloheximide (Cell Signaling Technology, #2112; 50 µg/mL for A549, 80 µg/mL for NCI‐H1299) was applied. Whole‐cell lysates were harvested at specified

(5) SIRT2-mediated deacetylation activates USP22 catalytic function for PD-L1 protein stabilization and tumor immune escapeJ Clin InvestJune 2, 2026Na Li, Qiong Gao, Huijun Jia, Guoqing Xue, Yuanzhang Zhou, Shengnan Wang, Suxian Ma, Bingjin Hu, Zhuoyue Zhao, Chen Su, et al.transfected HEK293T or T24 cells were treated with cycloheximide (CHX) (Cell Signaling Technology, 2112) at indicated time points. T24 cells were treated with 20 μM AGK2, 20 μM TM, or 20 μM SirReal (TargetMol,

(6) SIRT6‐Mediated Deacetylation of ATF3 Promotes Silica‐Induced Lung Fibrosis by Enhancing its Nuclear Import via Binding to Importin αAdvanced ScienceMay 20, 2026Demin Cheng, Wenxia Bu, Fengxu Wang, Yueyuan Jin, Rongzhu Liu, Rui Zhao, Xuehai Wang, Mengna Jiang, Jinping Shen, Xinhang Cheng, et al.fluorescence microscopy. 4.18 CHX Assay Cells were treated with 20 µm cycloheximide (Cell Signaling Technology, #2112) and harvested at 0, 3, 6, and 9 h post‐treatment. Whole‐cell lysates were prepared using RIPA buffer

(7) Proteasomal inhibition compromises microvascular integrity via distinct effects on non-immune endothelial cells and immune cellsFrontiers in ImmunologyMay 19, 2026Prajakta Sawant, Hannah Wolf, Aleena Mathew, Johanna Bensalel, Julio Gallego-Delgado, Pratyusha MandalALX-260-020-M005). TNF (Peprotech, Cat# 300-01B-1MG), Cycloheximide (Cell Signaling Technology, Cat# 2112), Staurosporine (Tocris, Cat# 1285) were used as needed for experiments. 2.2 Endothelial permeability

(8) Macrophage Gsα promotes NLRP3 stability and its intervention attenuates abdominal aortic aneurysm in male miceNature CommunicationsApril 2, 2026Lifan He, Xiaoteng Qin, Qingnan Ren, Hanlin Lu, Min Chen, Lee S. Weinstein, Cheng Zhang, Jianmin Yang, Xiangjiu Ding, Weiqiang Jing, et al.were obtained from Abcam. Detailed information is available in the Report Summary. Cycloheximide (CHX, 2112, Cell Signaling Technology), CQ (S6999, Selleck, Houston, USA), MG-132 (S2619, Selleck), MCC950 (Abmol,

(9) Mechanistic insights into SOCS5-related DNA damage and cellular senescence in diabetic retinopathyCell Death DiscoveryApril 1, 2026Di Yang, Siduo Lu, Hongmei Liu, You Zhou, Hua Zhongcultured for 20 h. The culture medium was then replaced with fresh medium containing 2–3 μg/mL CHX (2112, Cell Signaling) to inhibit de novo protein synthesis. Cells were harvested at 0, 3, 6, and 9 h after

(10) High-throughput screening identifies a trafficking corrector for long QT syndrome–associated KCNQ1 variantsJCI InsightMarch 9, 2026Katherine R. Clowes Clowes Moster, Carlos G. Vanoye, Ana C. Chang-Gonzalez, Ian M. Romaine, Katherine M. Stefanski, Mason C. Wilkinson, Joshua A. Bauer, Thomas P. Hasaka, Emily L. Days, Reshma R. Desai, et al.rate of KCNQ1 degradation, cells were then treated with either 50 μg/mL cycloheximide (Cell Signaling, 2112S) or 0.1% DMSO (as a control) for 0, 3, 6, or 9 hours. Soluble KCNQ1 was collected using the same lysis

(11) E3 ligase LMO7 enhances temozolomide sensitivity by promoting MGMT degradation in lung cancerJournal of Biological ChemistryFebruary 26, 2026Jiabing Li, Xiaorong Feng, Yunfang Deng, Bei Chen, Lin Miao, Zhaohui Liu, Zhiming Sun, Lei Hu, Jinqiu Ma, Liyuan Zeng, et al.(SA00001-1) (Proteintech). TMZ (S1237) was purchased from Selleck. CHX (180 μg/ml) was obtained from CST (2112S). The protease and phosphatase inhibitor cocktail was from Beyotime (P1051). Western blotting Cells

(12) USP20 governs tyrosine kinase inhibitors resistance through ferroptosis evasion by targeting GPX4 in cancersRedox BiologyFebruary 20, 2026Yuzhao Wang, Bo Liu, Yanxin Zhuang, Yulin Zhang, Weichao Dan, Peng Ding, Yi Wei, Chendong Guo, Mengxing Li, Chi Wang, et al.(cat. no. T2376), and MTT (cat. no. T19029) were purchased from TargetMol. Cycloheximide (CHX; cat. no. 2112) was purchased from CellSignaling Technology. Primary antibodies against USP20 (cat. no. PA5-85719;

(13) Dlgap2 deficiency disrupts synaptic homeostasis by promoting ubiquitin-mediated Itsn1 degradation in a valproic acid-induced autism-like modelScientific ReportsFebruary 11, 2026Xiaofeng Guo, Ling Zhang, Kai Zhuangchase assays Protein degradation was assessed using a CHX chase assay (Cell Signaling Technology, #2112). shDlgap2 interfering primary cortical neurons were treated with cycloheximide (150 ng/µl) and MG132

(14) Inhibitors supercharge kinase turnover through native proteolytic circuitsNatureJanuary 22, 2026Natalie S. Scholes, Martino Bertoni, Arnau Comajuncosa-Creus, Katharina Kladnik, Xuefei Guo, Fabian Frommelt, Matthias Hinterndorfer, Hlib Razumkov, Polina Prokofeva, Martin P. Schwalm, et al.(HY-12070) and GNF-2 (HY-11007). Cycloheximide (CHX) was purchased from Cell Signaling Technology (2112S). All compounds were dissolved in DMSO (Sigma-Aldrich, D1435) as 1 mM, 10 mM, 20 mM, 25 mM or 100 mM

(15) IGF2BP3 recognizes m6A to regulate histone-to-protamine replacement during mouse sperm developmentThe EMBO JournalDecember 5, 2025Dazhuang Wang, Zhenyi Huang, Yichun Zhou, Peiyan Chen, Gang Chang, Liwei Ke, Congying Jing, Haojie Yang, Jiexiang Zhao, Shaofang Ren, et al.cocktail,TargetMol,C0001 Recombinant RNasin® Ribonuclease Inhibitor,Promega,N2515 DNase I,NEB,M0303S Cycloheximide,CST,2112 TRIzol reagent,TIANGEN,Y1809 Hiscript III Reverse Transcriptase,Vazyme,R302-01 AceQ qPCR SYBR Green

(16) Targeting ubiquitin-specific protease 14 reduces metastatic potential and metabolic activity in cervical cancer via direct modulation of monocarboxylate transporter-4Journal of Translational MedicineDecember 2, 2025Ravi Chauhan, Gunjan Dagar, Lakshay Malhotra, Ashna Gupta, Anju Surendranath, Harshul Kapoor, Hemant Yadav, Rajat Ujjainiya, Suraja Kumar Das, Sameer Mirza, et al.in cell culture media for in vitro experiments. 10 mg/mL stock solution of Cycloheximide (Cat. No.: #2112, Cell Signaling Technology) was prepared by dissolving 50 mg of powder in 5 mL of DMSO and further diluted

(17) Palmitoylation of TBK1 enhances the type I interferon signaling and strengthens anti-malarial immunity in miceNature communicationsNovember 18, 2025Han Z, Xiong S, Zeng K, Xiao Z, Zhang L, Zhang Y, Guo J, Peng W, Xie Y, Liu W, et al.purchased from Selleck. MG132 (Cat#S2619) was purchased from Ambeed (A181909). Cycloheximide (CHX) (Cat#2112S) was purchased from Cell Signaling Technology. ML349 (Cat#HY-100737), protein A and G beads (Cat#HY-K0202)

(18) Cytoplasmic p21 promotes stemness of colon cancer cells via activation of the NFκB pathwayMolecular OncologyNovember 3, 2025Arnatchai Maiuthed, Kerstin Huebner, Katharina Erlenbach‐Wuensch, Chuanpit Hampel, Daniela Thalheim, Adriana Vial‐Roehe, Bodee Nutho, Susanne Merkel, Arndt Hartmann, Pithi Chanvorachote, et al.HCT116 cells were treated with the protein synthesis inhibitor cycloheximide (CHX; Cell Signaling Technology; #2112) and with the proteasome inhibitor MG132 (Sigma‐Aldrich, St. Louis, MO, USA; M7449) for the indicated

(19) Normal circadian period length requires repression of Npas2 by REV-ERB nuclear receptorsCell ReportsOctober 28, 2025Michael C. Tackenberg, Kristina M. Heliodoro, Lily C. Melink, Yifan Liu, Kun Zhu, Mitchell A. LazarScientific,A3582801 GlutaMAX,Thermo Scientific,35050061 Cycloheximide,Cell Signaling Technologies,2112S PF670462,Sigma,SML0795-5MG DMEM,Corning,90-013-PB ,, Deposited data,, ,, Control and Hepatocyte-specific

(20) High Throughput Screening Identifies a Small Molecule Trafficking Corrector for Long-QT Syndrome Associated KCNQ1 VariantsbioRxivOctober 15, 2025Katherine R. Clowes Clowes Moster, Carlos G. Vanoye, Ana C. Chang-Gonzalez, Ian M. Romaine, Katherine M. Stefanski, Mason C. Wilkinson, Joshua A. Bauer, Thomas P. Hasaka, Emily L. Days, Reshma R. Desai, et al.KCNQ1 degradation, cells were then treated with either 50 μg/mL cycloheximide (CHX) (Cell Signaling, 2112S) or 0.1% DMSO (as a control) for 0, 3, 6, or 9 hours. Soluble KCNQ1 was collected using the same lysis

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Cycloheximide from Cell Signaling Technology

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Cycloheximide from Cell Signaling Technology

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