Ionomycin, Calcium Salt from Cell Signaling Technology

Product Specs
ItemIonomycin, Calcium Salt
CompanyCell Signaling Technology
Price $303.00 Inquire
Catalog Number9995S
Quantity2.5 mg

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

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(1) NDRG3 is essential for sustaining antigen-driven T cell responses by protecting against restimulation-induced cell deathJ ImmunolMay 14, 2026Brian J Stack, Susan M KaechScientific Geneticin,10131027,Invitrogen "Trypsin-EDTA (0.25%), phenol red",25-200-056,Thermo Fisher Scientific PMA,P1585,MilliporeSigma Ionomycin,9995S,Cell Signaling Technology DMSO (dimethyl sulfoxide),25-950-CQC,Corning ACK buffer,RGF3015,Thermo Fisher

(2) TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cellsScientific reportsJuly 29, 2024Wünkhaus D, Tang R, Nyame K, Laqtom NN, Schweizer M, Scotto Rosato A, Krogsæter EK, Wollnik C, Abu-Remaileh M, Grimm C, et al.2418670-70-7), Bafilomycin A (Sigma, B1793), ML-SI3 (MedChemExpress, HY-139426), Ionomycin (Cell Signaling, 9995). For GPD synthesis, the following starting materials were purchases from Avanti: 18:1 Lyso PG (858125)

(3) Ca2+ transients on the T cell surface trigger rapid integrin activation in a timescale of secondsNature CommunicationsJuly 20, 2024Yue Li, ShiHui Wang, YouHua Zhang, ZhaoYuan Liu, YunZhe Zheng, Kun Zhang, ShiYang Chen, XiaoYing Lv, MengWen Huang, XingChao Pan, et al.min at room temperature, then followed by stimulation with 1 μM ionomycin (Cell Signaling Technology #9995 S), 0.5 μg/ml CCL25 or KCl/MOPS buffer control. Time-lapse images at a rate of one frame per 3 s were

(4) Gastrointestinal Dysmotility Predisposes to Colitis through Regulation of Gut Microbial Composition and Linoleic Acid MetabolismAdvanced science (Weinheim, Baden-Wurttemberg, Germany)May 1, 2024Zhang Y, Song F, Yang M, Chen C, Cui J, Xing M, Dai Y, Li M, Cao Y, Lu L, et al.stimulated with 20 ng mL−1 phorbol 12‐myristate 13‐acetate (PMA; Sigma), 1 µg mL−1 ionomycin (CST, 9995), and 1x brefeldin A (eBioscience, 00‐4506‐51) for 4 h at 37 °C. Cells were then washed, fixed by 2%

(5) The allergy mediator histamine confers resistance to immunotherapy in cancer patients via activation of the macrophage histamine receptor H1Cancer cellJanuary 10, 2022Li H, Xiao Y, Li Q, Yao J, Yuan X, Zhang Y, Yin X, Saito Y, Fan H, Li P, et al.Formaldehyde, Methanol-free",Pierce,Cat # 28906 "Ionomycin, Calcium Salt",Cell Signaling Technology,Cat # 9995; CAS: 56092-82-1 BAPTA-AM,Selleckchem,Cat # S7534; CAS: 126150-97-8 Recombinant Murine IFN-γ,PeproTech,Cat

(6) Castration-mediated IL-8 promotes myeloid infiltration and prostate cancer progressionNature cancerAugust 1, 2021Lopez-Bujanda ZA, Haffner MC, Chaimowitz MG, Chowdhury N, Venturini NJ, Patel RA, Obradovic A, Hansen CS, Jacków J, Maynard JP, et al.stimulated with PMA (50ng/ml; Thermo Fisher, Cat#NC9619504) and ionomycin (500ng/ml; Cell Signaling, Cat#9995S) for 4hrs in the presence of protein transport inhibitor cocktail (eBiosciences, Cat#00–4980-03). Gates

(7) Ubiquitin-specific peptidase 18 regulates the differentiation and function of Treg cellsGenes & diseasesMay 1, 2021Yang L, Jing Y, Kang D, Jiang P, Li N, Zhou X, Chen Y, Westerberg LS, Liu C.(50 ng/ml, Sigma, P1585-1 MG), GolgiStop (1:1000, BD Biosciences, 554,724) and ionomycin (1 μM, CST, 9995S). After culturing for 5 h in 37 °C, 5% CO2, cells were collected and stained with PE-anti-CD4 (Biolegend,

(8) Cytoneme delivery of Sonic Hedgehog from ligand-producing cells requires Myosin 10 and a Dispatched-BOC/CDON co-receptor complexeLifeFebruary 11, 2021Hall ET, Dillard ME, Stewart DP, Zhang Y, Wagner B, Levine RM, Pruett-Miller SM, Sykes A, Temirov J, Cheney RE, et al.red- and serum-free media supplemented with 0.083% DMSO (control), or DMSO with 2.5 µM ionomycin (#9995, CST). Image acquisition was performed with 60x/1.4NA/Oil objective lens (CFI Plan Apo Lambda) with

(9) B Cell Receptor Signaling and Protein Kinase D2 Support Regulatory B Cell Function in Pancreatic CancerFrontiers in immunologyJanuary 1, 2021Michaud D, Mirlekar B, Steward C, Bishop G, Pylayeva-Gupta Y.complete RPMI medium followed by the addition of PMA (50 ng/mL; #P8139, Sigma), Ionomycin (200 ng/mL; #9995S, Cell Signaling Technologies), and Brefeldin A (1x, #420601, BioLegend) for the final 5 hours at 37°C.

(10) STING couples with PI3K to regulate actin reorganization during BCR activationScience advancesApril 1, 2020Jing Y, Dai X, Yang L, Kang D, Jiang P, Li N, Cheng J, Li J, Miller H, Ren B, et al.ng/ml; Sigma-Aldrich, P1585-1MG), GolgiStop (1:1000; BD Biosciences, 554724), and ionomycin (1 μM; CST, 9995S) for 5 hours at 37°C, 5% CO2, and then cells were collected and stained with PE-anti-CD4 (BioLegend,

(11) Long-Term Programming of CD8 T Cell Immunity by Perinatal Exposure to GlucocorticoidsCellMarch 5, 2020Hong JY, Lim J, Carvalho F, Cho JY, Vaidyanathan B, Yu S, Annicelli C, Ip WKE, Medzhitov R.Biochemical,Cat#LS004186 Phorbol 12-myristate 13-acetate (PMA),Sigma-Aldrich,Cat#P1585 Ionomycin,Cell signaling technology,Cat#9995S Golgi plug,BD Biosciences,Cat#555029 Critical Commercial Assays,Critical Commercial Assays,Critical

(12) Rosmarinic acid alleviates cardiomyocyte apoptosis via cardiac fibroblast in doxorubicin-induced cardiotoxicityInternational Journal of Biological SciencesJanuary 1, 2019Xin Zhang, Jin-Xiu Zhu, Zhen-Guo Ma, Hai-Ming Wu, Si-Chi Xu, Peng Song, Chun-Yan Kong, Yu-Pei Yuan, Wei Deng, Qi-Zhu TangFas L Elisa Kit (RAB0179) were purchased from Sigma-Aldrich (St. Louis, MO, USA), whereas ionomycin (9995) was obtained from Cell Signaling Technology (Danvers, MA, USA). ApopTag® Plus In Situ Apoptosis Fluorescein Detection Kit

(13) A Comprehensive Roadmap of Murine Spermatogenesis Defined by Single-Cell RNA-SeqDevelopmental cellSeptember 10, 2018Green CD, Ma Q, Manske GL, Shami AN, Zheng X, Marini S, Moritz L, Sultan C, Gurczynski SJ, Moore BB, et al.Scientific,Cat#15632–011 SSC Buffer,ThermoFisher Scientific,Cat#15557–044 Ionomycin,Cell Signaling Technology,Cat#9995 PMA,Sigma,Cat#P1585 Fluorescent Nanodiamonds,Adamas Nano,Cat#NDNV100nmHi10ml Critical Commercial

(14) A sharp-edge-based acoustofluidic chemical signal generatorLab on a ChipMay 15, 2018Po-Hsun Huang, Chung Yu Chan, Peng Li, Yuqi Wang, Nitesh Nama, Hunter Bachman, Tony Jun Huangwas completed, the stimulant, which was a pure culture medium containing 2.0 μM ionomycin (Cat. No. 9995, Cell Signaling Technology, MA, USA), was injected into the channel through the ionomycin inlet (the

(15) Regulation of DLK-1 Kinase Activity by Calcium-Mediated Dissociation from an Inhibitory IsoformNeuronNovember 8, 2012Dong Yan, Yishi Jincell transfection. One day after transfection, cells were treated with 3 μM Ionomycin (Cell Signaling Technology #9995) with or without BAPTA-AM (10 mM) (Sigma #126150-97-8) for 15 min and lysed using radioimmunoprecipitation

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Ionomycin, Calcium Salt from Cell Signaling Technology

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Ionomycin, Calcium Salt from Cell Signaling Technology

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