LunaScriptRT Master Mix Kit (Primer-free) from New England Biolabs

Product Specs
ItemLunaScriptRT Master Mix Kit (Primer-free)
CompanyNew England Biolabs
Price $382.00 Inquire
Catalog NumberE3025L
Temperature-20 C
ApplicationsDNA Analysis
TypeMaster Mix
Quantity100 reactions

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Hepatitis C Virus 5'UTR Sequences That Bind eIF3 and Ribosomal 40S Subunits Confer Stimulation of Minus-Strand RNA SynthesisInt J Mol SciApril 2, 2026Attiya Qadoos Malik, Lyudmila Shalamova, Mozhdeh Khajouei, Jonas Budnik, Anna-Lena Hell, Elena Jost, Gesche K. Gerresheim, Oliver Rossbach, Michael NiepmannGermany; C10365); Monarch RNA isolation kit (NEB T2040L); LunaScript RT Master Mix Kit (Primer-free) (NEB E3025L); Luna Universal qPCR Master Mix (NEB M3003); Steady-Glo Luciferase Assay System (Promega, Walldorf,

(2) Protocol for validating computationally predicted splice-altering variants using full-length gene reporter assaysSTAR ProtocMarch 20, 2026Maria E. Lukes, Kostantin Kiianitsa, Anthony Widjaja, Olena Korvatska,Thermo Fisher Scientific,Cat# AM1907 LunaScript RT Master Mix Kit (Primer-free),New England Biolabs,Cat# E3025L Zymoclean Gel DNA Recovery Kit,Zymo Research,Cat# D4001 PureLink Quick PCR Purification Kit,Thermo Fisher Scientific

(3) Metabolic and behavioral effects of neurofibromin result from differential recruitment of MAPK and mTOR signalingPLoS GenetMarch 5, 2026Valentina Botero, Jenifer Barrios, Anneke Knauss, Greta Dahlen, Ethan Rosendahl, Kenneth J. Colodner, Seth M. TomchikComplimentary DNA library was constructed using LunaScript RT Master Mix (New England Biolabs, catalog #E3025L) with the Random Primer Mix (New England Biolabs, catalog #S1330S). Nf1 gene expression was quantified

(4) Metabolic and behavioral effects of neurofibromin result from differential recruitment of MAPK and mTOR signalingbioRxivJuly 31, 2025Valentina Botero, Jenifer Barrios, Anneke Knauss, Ethan Rosendahl, Kenneth J. Colodner, Seth M. TomchikComplimentary DNA library was constructed using LunaScript RT Master Mix (New England Biolabs, catalog #E3025L) with the Random Primer Mix (New England Biolabs, catalog #S1330S). Nf1 gene expression was quantified

(5) Choroid plexus apocrine secretion shapes CSF proteome during mouse brain developmentNature neuroscienceJuly 1, 2025Courtney Y, Head JP, Dani N, Chechneva OV, Shipley FB, Zhang Y, Holtzman MJ, Sadegh C, Libermann TA, Lehtinen MK.of RNA was reverse transcribed into cDNA using a LunaScript RT Master Mix kit (New England Biolabs, E3025L). qPCR reactions used specific primers and were performed in triplicate with Applied Biosystems TaqMan

(6) Targeted syndromic next-generation sequencing panel for simultaneous detection of pathogens associated with bovine reproductive failureJournal of Clinical MicrobiologyJanuary 31, 2025Dhinesh Periyasamy, Yanyun Huang, Janet E. HillLunaScript RT SuperMix Kit [cat. no. E3010L], and NEB LunaScript RT Master Mix Kit Primer-free [cat. no. E3025L] with BVDv-specific primers) were tested using manufacturer’s instructions with two BVDv-positive clinical

(7) Aedes aegypti D7 long salivary proteins modulate blood feeding and parasite infectionmBioNovember 1, 2023Ines Martin-Martin, Bianca Burini Kojin, Azadeh Aryan, Adeline E. Williams, Alvaro Molina-Cruz, Paola Carolina Valenzuela-Leon, Gaurav Shrivastava, Karina Botello, Mahnaz Minai, Zach N. Adelman, et al.modifications. RNA was reverse transcribed into cDNA using the LunaScript RT Master Mix Kit (Primer Free) (NEB, E3025L) with 6 µM Random Primer Mix (NEB, S1330S) following the manufacturer’s instructions in 20 µL total

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