LunaScriptRT Master Mix Kit (Primer-free) from New England Biolabs

Product Specs
ItemLunaScriptRT Master Mix Kit (Primer-free)
CompanyNew England Biolabs
Price $123.00 Inquire
Catalog NumberE3025S
Temperature-20 C
ApplicationsDNA Analysis
TypeMaster Mix
Quantity25 reactions

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Replicon-based genome-wide CRISPR knockout screening for the identification of host factors involved in viral replicationNat CommunDecember 10, 2025Karen W. Cheng, Madhura Bhave, Andrew L. Markhard, Duo Peng, Karan D. Bhatt, Katherine A. Travisano, Josette V. Medicielo, Astrid Anaya, Sanae Lembirik, Leila Njoya, et al.was performed using LunaScript RT supermix kits. The LunaScript RT supermix (primer-free) kit (NEB E3025S) was used to synthesize cDNA for EBOV vRNA, cRNA, and mRNA; a LunaScript RT supermix kit (NEB E3010S)

(2) KLF7 Blocks MKNK2/HIF-1 Pathway-Mediated M1 Microglia Polarization to Ameliorate Ischemic Stroke-Induced Neurological InjuryBrain and behaviorSeptember 1, 2025Wei R, Li S, Tang L.tissues or cells, and RNA was reverse transcribed into cDNA using the LunaScript RT Master Mix Kit (E3025S, New England Biolabs, Ipswich, MA, USA). Real‐time PCR was performed using the 7500 Fast Real‐Time PCR

(3) Assessing the Function of Porcine A Kinase-Interacting Protein 1 (AKIP1) In Vitro—A Central Regulator of Oxidative Stress and Mitochondrial FunctionsInt J Mol SciAugust 11, 2025Agnieszka Bak, Arne Hinrichs, Anna Schwaiger, Tobias Fromme, Andrea Fischer, Mayuko Kurome, Valeri Zakhartchenko, Barbara Kessler, Martin Klingenspor, Eckhard Wolf, et al.cDNA was synthesized based on the LunaScript RT Master Mix Kit (New England Biolabs, Germany, cat. no. E3025S) according to the manufacturer’s instructions. Relative gene expression levels were determined by quantitative real-time PCR

(4) Osteocalcin induces phosphorylation of FOXO1 in human beta-cells and restores insulin expression under hyperglycemic conditionsResearchSquareJune 13, 2025Shubhashish Sarkar, A. Osama Gaber, Christine A. Beamish, Omaima M. Sabekreverse transcribed by reverse transcriptase (RT) (NEB, LunaScript RT Master Mix Kit (Primer-free), E3025S) The cDNA obtained was diluted 1:10 in water and was used for PCR using specific primers (Table 2).

(5) High-throughput investigation of genetic design constraints in domesticated Influenza A Virus for transient gene deliverybioRxivFebruary 20, 2024Adam Sychla, Christopher S Stach, Shanley N Roach, Amanda N Hayward, Ryan A Langlois, Michael J SmanskiReverse Transcriptase PCR. Intracellular RNA was collected as above. The LunaScript Primer-Free RT Master Mix Kit (NEB E3025S) was used to generate cDNA per manufacturer protocol. Primers were used as listed in Supplementary Note

Be the First to Write a Review!