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Protein Biochemistry
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Coelenterazine 400a
Coelenterazine 400a from Cayman Chemical
Be the first to write a review!
Citations:
(7)
Supplier Page
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Cayman Chemical for
Coelenterazine 400a
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Description
A bisdeoxy derivative of coelenterazine that produces an emission maximum of 395 nm following conversion by Renilla luciferase; commonly used in BRET2 protocols to evaluate protein-protein interactions
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Coelenterazine 400a
Company
Cayman Chemical
Price
$194.00
Supplier Page
View Company Product Page
Catalog Number
16157
Format
A crystalline solid
Molecular Weight
391.5
Quantity
1 mg
Purity
≥95%
Origin
CAN
Formula
C26H21N3O
Storage
-20
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Cayman Chemical
Cayman Chemical Company 1180 East Ellsworth Road
Ann Arbor
,
Michigan
48108
United States
Phone
: (800) 364-9897
(734) 971-3640
Company Profile
Website:
http://www.caymanchem.com/
Citations (7)
(1)
Discovery and dynamic pharmacology of µ-opioid receptor positive allosteric modulators
bioRxiv
February 24, 2026
Evan S. O’Brien, Junzheng Wang, Parthasaradhireddy Tanguturi, Mengchu Li, Elizabeth White, Yuki Shiimura, Barnali Paul, Kevin Appourchaux, Kaavya Krishna Krishna Kumar, Weijiao Huang,
et al.
albumin), followed by the addition of 15 μL substrate buffer (40 μM
coelenterazine 400a
(
Cayman Chemical
,
16157
). This was incubated for 5 minutes in the dark at room temperature before reading. The plates were read
(2)
BRET-Based Self-Cleaving Biosensors for SARS-CoV-2 3CLpro Inhibitor Discovery
Microbiology Spectrum
August 31, 2022
Ningke Hou, Chen Peng, Lijing Zhang, Yuyao Zhu, Qi Hu
transferred to a 96-well clear-bottom white plate (
Corning
,
3610
). After adding
coelenterazine 400a
(
Cayman
,
16157
) to a final concentration of 20 μM, the luminous signal at 410 nm and the fluorescent signal at 515
(3)
Ligand-directed bias of G protein signaling at the dopamine D2 receptor
Cell Chemical Biology
February 1, 2022
Ee Von Von Moo, Kasper Harpsøe, Alexander S. Hauser, Ikuo Masuho, Hans Bräuner-Osborne, David E. Gloriam, Kirill A. Martemyanov
System,
Promega
,Cat#
N2420
[3H]Spiperone,
Perkin Elmer
,Cat#
NET1187250UC
Coelentrazine 400a,
Cayman Chemicals
,Cat#
16157
Deposited data,Deposited data,Deposited data β1 structure,"(Warne et al., 2019)",PDB ID 6H7J
Aripiprazole
(4)
Bioluminescence Resonance Energy Transfer 2 (BRET2)‐Based RAS Biosensors to Characterize RAS Inhibitors
Current Protocols in Cell Biology
June 1, 2019
Nicolas Bery, Terence H. Rabbitts
10010023
) OptiMEM no red phenol (
Thermo Fisher
, cat. no.
11058021
)
Coelenterazine 400a
(
Cayman Chemicals
, cat. no.
16157
) 100% Ethanol (EtOH) RAS
biosensors
plasmids [(Bery et al., 2018); see Table 1]
pEF-myc-cyto
, empty
(5)
Small molecule inhibitors of RAS-effector protein interactions derived using an intracellular antibody fragment
Nature communications
August 9, 2018
Quevedo CE, Cruz-Migoni A, Bery N, Miller A, Tanaka T, Petch D, Bataille CJR, Lee LYW, Fallon PS, Tulmin H,
et al
.
directly after addition of
Coelenterazine 400a
substrate (10 μM final) to cells (
Cayman Chemicals
, cat#
16157
). BRET2 reading was carried out on an Envision instrument (2103 Multilabel Reader, PerkinElmer) with
(6)
BRET-based RAS biosensors that show a novel small molecule is an inhibitor of RAS-effector protein-protein interactions
eLife
July 10, 2018
Bery N, Cruz-Migoni A, Bataille CJ, Quevedo CE, Tulmin H, Miller A, Russell A, Phillips SE, Carr SB, Rabbitts TH.
(EGF),
Life Technologies
,Cat#
PHG0311
, "Chemical compound, drug",
Coelenterazine 400a
,
Cayman Chemical
,Cat#
16157
, "Chemical compound, drug",2-bromo-6-methoxyphenol,This paper,N/A, "Chemical compound, drug","3-bromobenzene-1,2-diol",This
(7)
Structural and functional characterization of a DARPin which inhibits Ras nucleotide exchange
Nature communications
July 14, 2017
Guillard S, Kolasinska-Zwierz P, Debreczeni J, Breed J, Zhang J, Bery N, Marwood R, Tart J, Overman R, Stocki P,
et al
.
directly after addition of
Coelenterazine 400a
substrate (10 μM final) on cells (
Cayman Chemicals
, cat#
16157
) on an Envision instrument (2103 Multilabel Reader, PerkinElmer) with the BRET2 Dual Emission optical
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Coelenterazine 400a from Cayman Chemical
Supplier Page
View Company Product Page
$194.00
Product Specs
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