MTT from Cayman Chemical

Product Specs
ItemMTT
CompanyCayman Chemical
Price $297.00Supplier Page View Company Product Page
Catalog Number21795
FormatA crystalline solid
Research AreaApoptosis, Cell Biology, Cell Death
Molecular Weight414.3
Quantity5 g
Purity≥95%
Storage-20
FormulaC18H16N5S • Br
OriginCHN

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Cayman Chemical

Cayman Chemical Company 1180 East Ellsworth Road

Ann Arbor, Michigan 48108

United States

Phone: (800) 364-9897
(734) 971-3640

Company Profile

Website: http://www.caymanchem.com/

(1) Zeaxanthin Mitigates Hydroxychloroquine-Induced Retinal Toxicity and Metabolic Reprogramming in ARPE-19 CellsJ Biochem Mol ToxicolJuly 8, 2026Münire Berna Asal Altıparmak, Emine Merve Yıldırım, Ozan Kaplan, Emine Koç, Açelya Erikçi, Mustafa Çelebier, Tuba Tüylü Küçükkılınç, Gülberk Uçarincubation. MTT assay was performed to assess cytotoxicity. Briefly, 10 µL of MTT reagent (5 mg/mL, Cayman 21795) was added to each well after incubation. The formed MTT formazan crystals were dissolved by adding

(2) Lipid droplets and small extracellular vesicles interplay in Japanese encephalitis virus non-lytic releasemBioJune 10, 2026Bhaghyasree Mallick, Ananya Mondal, Ankita Sarkar, Tamoghna Chakraborty, Khadijah Khan, Dilip Kumar, Subhas Chandra Biswas, Sourish GhoshHypotonic lysis buffer,22050012,bioWORLD,India Insulin,I6654,Sigma-Aldrich,Germany Laemmli,3016242,Invitrogen,USA LB agar,M1151,HIMEDIA,India LB broth,M1245,HIMEDIA,India MTT,21795,Cayman,USA 2-Mercaptoethanol,M3148,Sigma,USA Neurobasal medium,211003-049,Thermo Scientific,USA Nitrocellulose membrane

(3) Increased vulnerability of colorectal cancer models with high alkylation damage to NTHL1 inactivationScientific ReportsApril 21, 2026Hyeoncheol Kim, Jules Cazaubiel, Tung-Lin Chiang, Dimitrios Stylianakis, Samantha Fitzgerald, Pushpamali De Silva, Carino Gurjao, Marios GiannakisAZD7762 (1 µM), Prexasertib (5 µM), or Rabusertib (5 µM) for 48 h. Following treatment, 1 mg/mL MTT (21795, Cayman, Ann Arbor, Michigan) was added to each plate and incubated for 1 h. Dimethyl sulfoxide (A13280.0E,

(4) TBXA2R activates ERMs to drive motility, invasion, and metastatic colonization of TNBC cellsLife Sci AllianceMarch 17, 2026Kévin Leguay, Omaima Naffati, Camila Lie Kiyan, Yu Yan He, Mireille Hogue, Chloé Tesnière, Melania Gombos, Hellen Kuasne, Louis Gaboury, Christian Le Gouill, et al.in the figure legend. The medium was then replaced with fresh medium supplemented with 1.1 mM MTT (#21795; Cayman Chemical), and cells were incubated at 37°C for 4 h. Dissolution of formazan was then performed

(5) Myofibroblast lineage mapping and inhibiting subretinal fibrosis by targeting SMAD3 and MRTF pathways via microRNA-24 functional studybioRxivMarch 4, 2026Yinga Wu, Yao Tong, Katherine G. Byrnes, Qi Zhou, Chunmin Dong, Chase Benjamin, Emma Parker, Duran Bao, Zhaoyang Ren, Chastain A. Anderson, et al.and infected with Ad-miR-24 or control virus. Four days later, MTT reagent (1 mg/mL; Cayman Chemical, 21795) was added and incubated for 4 h at 37 °C. Formazan crystals were dissolved in DMSO, and absorbance

(6) Epstein-Barr Virus Promotes Gastric Cancer Progression by Modulating m6A-Dependent YTHDF1-TSC22D1 AxisMicroorganismsDecember 11, 2025Yea Rim An, Jaehun Jung, Kyeong Min Kwon, Jun Yeob Kim, Min-Hyeok Lee, Ju Yeon Lee, Minho Lee, Suk Kyeong Leeexperiments. 2.8. MTT Assay (Cell Viability Assay) Cell proliferation was analyzed using an MTT assay (#21795, Cayman Chemical, Ann Arbor, MI, USA). AGS (5 × 103), SNU-216 (1 × 104), AGS-EBV (7.5 × 103), and YCCEL1

(7) Uremic serum exposure leads to differential phenotypic switch in porcine arterial and venous smooth muscle cellsAmerican Journal of Physiology-Renal PhysiologyDecember 1, 2025Huanjuan Su, Unimunkh Uriyanghai, Christine Wai, Eyla C. Arteaga, Haibao Wan, Klaus-Peter Adam, Vinay A. Sudarsanam, Samuel O’Brien Haddad, Anthony Yang, Lianxia Li, et al.Cell Signaling Technology, Inc. (Danvers, MA). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Cat# 21795) was purchased from Cayman Chemical (Ann Arbor, MI). Nitrocellulose membrane was obtained from Thermofisher

(8) Characterization of Protein Expression and Signaling Pathway Activation That May Contribute to Differential Biological Functions in Porcine Arterial and Venous Smooth Muscle CellsInternational journal of molecular sciencesMarch 28, 2025Arteaga EC, Wai C, Uriyanghai U, Rudraraju M, Su H, Sudarsanam VA, Haddad SO, Poulton JS, Roy-Chaudhury P, Xi G.USA). The anti-TIMP-3 antibody (Orb214673) was purchased from Biorbyt (Durham, NC, USA). MTT (Cat# 21795) was purchased from Cayman Chemical (Ann Arbor, MI, USA). All other reagents were obtained from MilliporeSigma

(9) Oxidized Low-Density Lipoprotein Induces Reactive Oxygen Species-Dependent Proliferation of Intestinal Epithelial CellsPharmaceuticals (Basel)November 1, 2024Eddy E. Gonzalez-Horta, Juan F. Burgueno, María J. Leiva, Carla Villavicencio, Fernando I. Kawaguchi, Hajar Hazime, Fátima Reyes, Viana Manrique-Suárez, Natalie C. Parra, Maria T. Abreu, et al.with 0.5 mg/mL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Cayman Chemical, 21795, Ann Arbor, MI, USA) for 1–2 h at 37 °C to assess cell viability. This assay quantifies metabolically

(10) Dysregulation of mitochondrial translation caused by CBFB deficiency cooperates with mutant PIK3CA and is a vulnerability in breast cancerCancer ResApril 14, 2023Navdeep Malik, Young-Im Kim, Hualong Yan, Yu-Chou Tseng, Wendy du Bois, Gamze Ayaz, Andy D. Tran, Laura Vera-Ramirez, Howard Yang, Aleksandra M. Michalowski, et al.72 hours after the treatment, cell viability was analyzed using a MTT-based (Cayman chemicals, Cat#21795) cell viability assay. IC50 values for each drug alone or in combination were computed using GraphPad

(11) Regulation of programmed death ligand 1 (PD‐L1) expression by TNF‐related apoptosis‐inducing ligand (TRAIL) in triple‐negative breast cancer cellsMolecular CarcinogenesisFebruary 1, 2023Julio M. Pimentel, Jun‐Ying Zhou, Gen Sheng Wu9542) were purchased from Cell Signaling. MTT was purchased from Cayman Chemical Company (catalog. No 21795). siGenome SMARTpool Human MAPK1 (catalog no. M-003555-04), siGenome SMARTpool Human MAPK3 (catalog

(12) Antibacterial and Proliferative Effects of NaOH-Coated Titanium, Zirconia, and Ceramic-Reinforced PEEK Dental Composites on Bone Marrow Mesenchymal Stem CellsPharmaceuticsDecember 28, 2022Artiom Lijnev, Jeevithan Elango, Vicente M. Gómez-López, Carlos Pérez-Albacete Martínez, José Manuel Granero Marín, José Eduardo Maté Sánchez De ValNext, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Cayman chemical company, Ref. 21795) reagent (400 µL) was added into wells for 2 h, and then the formazan dye was solubilized with 200 µL

(13) The UCHL5 inhibitor b-AP15 overcomes cisplatin resistance via suppression of cancer stemness in urothelial carcinomaMolecular therapy oncolyticsSeptember 15, 2022Chow PM, Dong JR, Chang YW, Kuo KL, Lin WC, Liu SH, Huang KH.indicated time or received no treatment. After treatment, cell viability was determined using an MTT (#21795, Cayman Chemical, Ann Arbor, MI, USA) assay by incubating the cells with 500 μg/mL MTT in culture medium

(14) Programmable human histone phosphorylation and gene activation using a CRISPR/Cas9-based chromatin kinaseNature CommunicationsDecember 1, 2021Jing Li, Barun Mahata, Mario Escobar, Jacob Goell, Kaiyuan Wang, Pranav Khemka, Isaac B. HiltonPLX-4720, in 200 µl. MTT reagent (3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide; Cayman, 21795-1) was then added to a final concentration of 0.5 mg/ml. The reaction was incubated at 37 °C for 3 h

(15) A light-switching pyrene probe to detect phase-separated biomoleculesiScienceAugust 20, 2021Masaharu Hazawa, Shogo Amemori, Yoshio Nishiyama, Yoshihiro Iga, Yuki Iwashima, Akiko Kobayashi, Hirohisa Nagatani, Motohiro Mizuno, Kenji Takahashi, Richard W. WongChmicals,Cat#161-23181 "MTT 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium, monobromide",Cayman Chemical,Cat#21795 DMF,Nacalai Tesque,Cat#13016-65 Paraformaldehyde,Nacalai Tesque,Cat#26123-55 BSA,FUJIFILM Wako Pure

(16) Activation of lysosomal iron triggers ferroptosis in cancerNatureJanuary 1, 0001"Cañeque T, Baron L, Müller S, Carmona A, Colombeau L, Versini A, Solier S, Gaillet C, Sindikubwabo F, Sampaio JL, et al."the medium was carefully removed and 50 μl serum-free medium and 50 μl MTT solution (Cayman Chemical, 21795) were added per well. The plate was incubated at 37 °C for 3 h. After incubation, the solution was removed

(17) The ORP9-ORP11 dimer promotes sphingomyelin synthesiseLifeJanuary 1, 0001"Cabukusta B, Borst Pauwels S, Akkermans JJLL, Blomberg N, Mulder AA, Koning RI, Giera M, Neefjes J."methyl-β-cyclodextrin (Sigma #C4555) and cell viability was tested using MTT viability assay (Cayman #21795). Absorbance at 560 nm was measured using a BMG ClarioStar plate reader. Liposome preparations For

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Cayman Chemical

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MTT from Cayman Chemical

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