Protease Inhibitor Cocktail (100X) from Abcam

Product Specs
ItemProtease Inhibitor Cocktail (100X)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab271306
Quantity3 mL, 10 mL, 30 mL
TypeInhibitor

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Abcam

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United Kingdom

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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) DUSP11 is an Intracellular Innate Immune Checkpoint in Lung AdenocarcinomaCancer Immunology ResearchNovember 3, 2025Brian J. Thomas, Xue Bai, Benjamin J. Cryer, Sydney M. Escobar, Lee-Ann H. Allen, Mark A. Daniels, Margaret J. Lange, Donald H. Burkedetection reagents (cat #22660) were purchased from ThermoFisher Scientific (Waltham, MA). Protease (cat #ab271306) and phosphatase (cat #ab201112) inhibitor cocktails and RIPA buffer (cat #ab156034) were purchased

(2) DUSP11 is an intracellular innate immune checkpoint in lung adenocarcinomaCancer Immunol ResNovember 3, 2025Brian J. Thomas, Xue Bai, Benjamin J. Cryer, Sydney M. Escobar, Lee-Ann H. Allen, Mark A. Daniels, Margaret J. Lange, Donald H. Burkedetection reagents (cat #22660) were purchased from ThermoFisher Scientific (Waltham, MA). Protease (cat #ab271306) and phosphatase (cat #ab201112) inhibitor cocktails and RIPA buffer (cat #ab156034) were purchased

(3) Specificity and mechanism of tRNA cleavage by the AriB Toprim nuclease of the PARIS bacterial immune systemPhilosophical Transactions of the Royal Society B: Biological SciencesSeptember 4, 2025Svetlana Belukhina, Baptiste Saudemont, Florence Depardieu, Tom Lorthios, Tinashe P. Maviza, Alexei Livenskyi, Marina Serebryakova, Maria Aleksandrova, Elizaveta Ukholkina, Nadezhda Burmistrova, et al.buffer A (100 mM Tris pH 8.0, 150 mM NaCl, 1 mM EDTA) containing protease inhibitor cocktail (Abcam, ab271306). Cells were lysed by sonication on ice and cell debris was removed by centrifugation (15 000g, 20 min,

(4) Cinnamic Acid: A Shield Against High-Fat-Diet-Induced Liver Injury-Exploring Nrf2's Protective MechanismsInternational journal of molecular sciencesAugust 17, 2025Alahdal AT, Al-Harbi LN, Alshammari GM, Saleh A, Yahya MA.This tissue/PBS mixture was further supplemented with a protease and phosphatase inhibitor cocktail (ab271306, Abcam, Cambridge, UK) to achieve a final volume of 500 µL, thereby preserving protein stability. The

(5) Sox9 in the epicardium: Implications for cell invasion, differentiation, and coronary vascular developmentPLoS OneJune 23, 2025Andrew B. Harvey, Allison M. Trouten, Renélyn A. Wolters, Jenna R. Drummond, Raymond N. Deepe, Hannah G. Tarolli, Inara Devji, Silvia G. Vaena, Martin J. Romeo, Robin Muise-Helmericks, et al.dissected embryonic hearts using 1X RIPA buffer (Thermo, 89900) with 1X Protease Inhibitor Cocktail (Abcam, ab271306), boiled at 95 °C for 5 mins with 4X Laemmli Buffer (BioRad, 1610747) with 10% 2-mercaptoethanol, and

(6) Increased Expression of Synaptic Vesicle Glycoprotein 2A (SV2A) in the Brain of Chronic Diabetic RatsSynapseMay 3, 2025Burcu Azak Pazarlar, Cansu Bilister Egilmez, Eser Öz Oyar, Jens D. Mikkelsenbuffer (150 mM NaCl, 1% Triton X‐100, and 10 mM Tris, pH 7.4) containing a protease inhibitor (Abcam, ab271306). The homogenate was centrifuged at 10,000 × g for 10 min at 4°C and the supernatant was collected for

(7) Purple Sweet Potato Ameliorates High-Fat Diet-Induced Visceral Adiposity by Attenuating Inflammation and Promoting Adipocyte BrowningJournal of agricultural and food chemistryFebruary 12, 2025Yen CH, Chiang MH, Lee YC, Kao ES, Lee HJ.homogenized in 1000 μL of radioimmunoprecipitation assay buffer containing 10 μL of protease inhibitors (ab271306, Abcam Ltd., Cambridge, UK) using a homogenizer (Bertin, K0668, France). The homogenate was centrifuged

(8) Modulation of Extrinsic and Intrinsic Signaling Together with Neuronal Activation Enhances Forelimb Motor Recovery after Cervical Spinal Cord InjuryeNeuroFebruary 7, 2025Hirohide Takatani, Naoki Fujita, Fumiyasu Imai, Yutaka Yoshida150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, and protease inhibitor cocktail (Abcam, ab271306)]. After centrifugation at 10,000 × g for 10 min at 4°C, the protein concentration was adjusted to 1

(9) Modulation of Extrinsic and Intrinsic Signaling Together with Neuronal Activation Enhances Forelimb Motor Recovery after Cervical Spinal Cord InjuryeNeuroFebruary 7, 2025Hirohide Takatani, Naoki Fujita, Fumiyasu Imai, Yutaka Yoshida150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, and protease inhibitor cocktail (Abcam, ab271306)]. After centrifugation at 10,000 × g for 10 min at 4°C, the protein concentration was adjusted to 1

(10) Curcumin nanoparticles alleviate brain mitochondrial dysfunction and cellular senescence in γ-irradiated ratsScientific ReportsJanuary 31, 2025Omnia A. Moselhy, Nahed Abdel-Aziz, Azza El-bahkery, Said S. Moselhy, Ehab A. Ibrahimand then homogenized in mitochondria isolation buffer with a protease inhibitor cocktail (CAT. NO. ab271306, Abcam, UK) added to prevent protein degradation at a ratio of 1:5 (W/V). The homogenate was centrifuged

(11) β-hydroxybutyrate is a metabolic regulator of proteostasis in the aged and Alzheimer disease brainCell Chemical BiologyJanuary 16, 2025Sidharth S. Madhavan, Stephanie Roa Roa Diaz, Sawyer Peralta, Mitsunori Nomura, Christina D. King, Kaya E. Ceyhan, Anwen Lin, Dipa Bhaumik, Anna C. Foulger, Samah Shah, et al.cold TEM buffer (50 mM Tris, 1 mM EDTA, 0.5 mM MgCl2, pH 7.4) + 1x protease inhibitor cocktail (Abcam ab271306) with 30 up-and-down strokes on ice. Homogenate was transferred by pipetting into a 1.5 mL microtube

(12) Therapeutic efficacy of rhoifolin in type 2 diabetes mellitus: Effects on metabolic parameters, hepatic function, and oxidative stress: a dose-dependent studyFrontiers in pharmacologyJanuary 1, 2025Fattiny SZ, Binobead MA, Yagoub AEA, Alshammari GM, Saleh A, Abdo Yahya M.(w/v) was prepared and supplemented with 1.5 µL of protease and phosphatase inhibitor cocktail (code #ab271306, Abcam, Cambridge, UK) to preserve protein integrity. The liver tissue (100 mg) was cut into small fragments

(13) The tubulin polymerization inhibitor gambogenic acid induces myelodysplastic syndrome cell apoptosis through upregulation of Fas expression mediated by the NF-κB signaling pathwayCancer biology & therapyDecember 31, 2024Zhong C, Wang S, Xia L, Yang X, Fang L, Zhang X, Wang M, Zhao H, Wang G, Wu J, et al.Tris-HCl, pH 7.4, 150 mm NaCl, 1 mm EDTA, 1% Triton X-100) plus a protease inhibitor cocktail (Abcam, ab271306) for 60 min on ice. Proteins were separated on a 4–20% SDS-polyacrylamide gradient gel and transferred

(14) Isoliquiritigenin Prevents the Development of Nephropathy by an HFD in Rats Through the Induction of Antioxidant Production and Inhibition of the MD-2/TLR4/NF-κB PathwayBiology (Basel)November 28, 2024Mohammed Abdo Yahya, Ghedeir M. Alshammari, Magdi A. Osman, Laila Naif Al-Harbi, Setah Naif Alotaibi(PBS) at a ratio of 1:10 (w/v) and supplemented with a protease and phosphatase inhibitor cocktail (ab271306, Abcam, Cambridge, UK) to achieve a final volume of 500 µL, thereby preserving protein stability. The

(15) FXR1 associates with and degrades PDZK1IP1 and ATOH8 mRNAs and promotes esophageal cancer progressionBiology DirectNovember 7, 2024Faiz Ali Khan, Dalia Fouad, Farid S. Ataya, Na Fang, Jingcheng Dong, Shaoping JiWestern blot analysis The cells were lysed in RIPA lysis buffer, including the complete protease inhibitor cocktail (Abcam, ab271306). Protein concentration was determined using a BCA reagent (Thermo Scientific, Cat#23225). An equal

(16) Protective Effect of Whey Protein Supplement Against Rotenone Induced Motor Dysfunction in a Rat Model of Parkinson DiseaseAdv Biomed ResOctober 28, 2024Parvin Zarei, Behnam Amirpour-Najafabadi, Parnian Sam-Sani, Mohammad Hassan Sakhaie, Mehdi Sadeghhomogenized in 1000 µl of phosphate buffer at pH 7.4, along with a protease inhibitor (Abcam, USA: ab271306). The homogenate was centrifuged at 14,000 rpm for 40 minutes at 4°C, after which the supernatant was

(17) Royal Jelly Exerts a Potent Anti-Obesity Effect in Rats by Activating Lipolysis and Suppressing AdipogenesisNutrientsSeptember 19, 2024Alaa Hasanain Felemban, Ghedeir M. Alshammari, Abu ElGasim Ahmed Yagoub, Ali Saleh, Mohammed Abdo Yahyaavoid protein degradation and phosphatase activation, 1.5 µL of a protease inhibitor cocktail (Cat. #ab271306, Abcam, Cambridge, UK) and 1.5 µL of a phosphatase inhibitor cocktail (Cat. #78420, ThermoFisher, Waltham,

(18) N-Acetylcysteine Alleviates Necrotizing Enterocolitis by Depressing SESN2 Expression to Inhibit Ferroptosis in Intestinal Epithelial CellsInflammationJuly 22, 2024Chuchu Gao, Lixia Wang, Kai Fu, Shan Cheng, Sannan Wang, Zongtai Feng, Shenglin Yu, Zuming Yangreverse (R) primers are provided in Table 3. Western Blot Analysis A protease inhibitor cocktail (ab271306, Abcam, Cambridge, UK) was added to RIPA buffer (Beyotime, Shanghai, China) for the extraction of total

(19) Fatty acid binding protein 5 suppression attenuates obesity-induced hepatocellular carcinoma by promoting ferroptosis and intratumoral immune rewiringNature metabolismApril 1, 2024Sun J, Esplugues E, Bort A, Cardelo MP, Ruz-Maldonado I, Fernández-Tussy P, Wong C, Wang H, Ojima I, Kaczocha M, et al.P-40, 0.5% sodium deoxycholate and 0.1% SDS) supplemented with protease inhibitor cocktail (Abcam; ab271306). Cell lysates were rotated at 4 °C for 1 h before the insoluble material was removed by centrifugation

(20) A multifunctional Wnt regulator underlies the evolution of rodent stripe patternsNature ecology & evolutionDecember 1, 2023Johnson MR, Li S, Guerrero-Juarez CF, Miller P, Brack BJ, Mereby SA, Moreno JA, Feigin CY, Gaska J, Rivera-Perez JA, et al.0.5% sodium deoxycholate, 0.1% Triton-X, 1 mM EDTA) supplemented with protease inhibitor cocktail (ab271306, Abcam). Protein concentration was quantified by Pierce Detergent Compatible Bradford Assay (Thermo Fisher Scientific

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Protease Inhibitor Cocktail (100X) from Abcam

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Protease Inhibitor Cocktail (100X) from Abcam

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