AR6 buffer, 10X, 250 mL from Akoya Biosciences, a Quanterix company

Product Specs
ItemAR6 buffer, 10X, 250 mL
CompanyAkoya Biosciences, a Quanterix company
Price $111.08Supplier Page View Company Product Page
Catalog NumberAR600250ML
Quantity1
ApplicationsMultiplex IHC
Typebuffer

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Akoya Biosciences, a Quanterix company

900 Middlesex Turnpike

Building 1

Billerica, MA 01821

United States

Phone: +1 (617) 765-7943

Email: sales@quanterix.com

Company Profile

Website: quanterix.com

(1) Persistent glycolysis defines pathological foreign body-associated inflammation to polymeric implantsSci AdvDecember 19, 2025Christian Rempe, Neal Callaghan, Lauren Fong-Hollohan, Sarah Nersesian, Zachary Froom, Kyle Medd, Ibrahim Ahmed, Tobias Karakach, Jeanette E. Boudreau, Michael Bezuhly, et al.Alexa Fluor 700 Conjugation Kit (Fast)–Lightning-Link (Abcam, AB269824), AR6 Antigen Retrieval Buffer [1 to 5% (w/w) sodium citrate; Akoya Biosciences, AR600250ML], AR9 Antigen Retrieval Buffer [1 to 2.5% tris (w/w) and <1% EDTA (w/w); Akoya Biosciences, AR900259ML],

(2) Differentiation grade is highly concordant between matched primary and metastatic colorectal cancerClin Exp MetastasisOctober 17, 2025Camilla M. Reehorst, Jennifer K. Mooi, Charles J. Uy, Kristen A. Needham, Sarah Ellis, Ian Y. Luk, Laura J. Jenkins, Rebecca Nightingale, Fiona Chionh, Andreas Behren, et al.submerging the slides in pH9 or pH6 antigen retrieval (AR) buffer (Akoya Biosciences, AR900250ML and AR600250ML, Akoya Biosciences) and microwaving at 98 °C (20% power) for 15 min. Slides were washed with Tris-buffered

(3) SARC028 samples reveal an interplay between TGFβ, interferon signaling and low HLA class I expression as contributors to Ewing sarcoma checkpoint blockade resistanceClinical Cancer ResearchSeptember 2, 2025Jessica D. Daley, Elina Mukherjee, David Ferraro, Shanthi Bhaskar, Anthony Green, Ernest M. Meyer, Hussain Tawbi, Melissa Burgess, Tullia C. Bruno, Anthony R. Cillo, et al.formalin-fixed, paraffin-embedded tumor tissue was deparaffinized. AR6 (Akoya Biosciences, catalog No. AR600250ML) was used for heat-induced antigen retrieval in serial cycles to stain tissues for CD68 (1:800, Cell Signaling Technology

(4) Methylation-induced suppression of BEX1 activates AKT/ERK/STAT3 signaling pathways regulating cell cycle and apoptosis in gliomaSci RepAugust 13, 2025Li-Zhi Xiao, Shi Feng , Zhen He, Rui Mao, Kai Qian, Zhi-Ying Zhou, Huabao Cai, Mengyu Zhao, Cunzhi Wang, Tianhang Yu, et al.deparaffinized, rehydrated, and subjected to antigen retrieval in AR6 buffer (Akoya Biosciences, Cat. No. AR600250ML). Sequential staining was performed using primary antibodies against p-STAT3 (CST, Cat. No. 9145, 1:100),

(5) Renal prognostic value of serum monoclonal immunoglobulin in cryoglobulinemic glomerulonephritisFrontiers in ImmunologyJuly 29, 2025Lei Ma, Yuanyuan Xia, Yun Fan, Dan Zhou, Xinchen Yao, Yongzhong Zhong, Fan Yang, Feng Xu, Shaoshan Liang, Yujie Wang, et al.water for 3 min. Antigen retrieval was then performed using citrate buffer (pH 6.0) (Akoya Biosciences, AR600250ML). Endogenous peroxidase activity was blocked with a peroxidase-blocking solution (Beyotime Biotechnology,

(6) Enhanced IL36RN Expression and Its Association With Immune Microenvironment Predicts Poor Prognosis in Gastric CancerCancer medicineMay 1, 2025Zhang X, Jiang S, Yin H, Zhang H, Yang L, Sun P, Kuai X, Chen C, Huang J.[21]. Antigen retrieval was performed using AR6 buffer (AR900250ML, AKOYA, Boston, USA) and AR9 buffer (AR600250ML, AKOYA, Boston, USA). Sequential rounds of staining were carried out, followed by nuclear counterstaining

(7) Association of peripheral monocytic myeloid-derived suppressor cells with molecular subtypes in single-center endometrial cancer patients receiving carboplatin + paclitaxel/avelumab (MITO-END3 trial)Cancer Immunol ImmunotherApril 17, 2025C. D'Alterio, G. Rea, M. Napolitano, E. Coppola, A. Spina, D. Russo, R. Azzaro, C. Mignogna, G. Scognamiglio, D. Califano, et al.Opal fluorescence signal in a decloaking chamber for 20 min 95 °C with AR6 Buffer AKOYA biosciences (AR600250ML); a second run was conducted with Recombinant CD14 (EPR3653) Rabbit Monoclonal (Cell Marque Cat# 114R,

(8) Tissue-resident CCR2+ macrophage TREM-1/3 signaling is necessary for monocyte and neutrophil recruitment to injured heartsCell ReportsMarch 25, 2025Yuriko Terada, Wenjun Li, Junedh M. Amrute, Amit I. Bery, Charles R. Liu, Venkatrao Nunna, Christian Corbin Frye, Hao Dun, Andrew L. Koenig, Hannah P. Luehmann, et al.Protein Transport Inhibitor (Containing Brefeldin A),BD Biosciences,Cat# 555029 10X AR6 buffer,Akoya Biosciences,Cat# AR600250ML "TRIS-buffered saline (TBS, 20X), with 2% Tween 20",Fisher Scientific,Cat# J60497.K3 "VECTASTAIN ®

(9) α7-Nicotinic Acetylcholine Receptor and Mutated α-Synuclein Interact in Motor Behaviour and Nigrostriatal Dopamine-Findings With Potential Relevance for a Protective Effect of Cigarette Smoking and Parkinson's DiseaseEur J NeurosciMarch 24, 2025Christian Pifl, Alexandra Wolf, Mark Elevado, Petra Scholzeintroducing the next primary antibody, α‐Syn sections were steamed again using AR6 (Akoya Biosciences Inc. #AR600250ML) for 30 min. After blocking step, α‐Syn was incubated over night at 4°C. Detection was done with secondary

(10) Gemistocytic tumor cells programmed for glial scarring characterize T cell confinement in IDH-mutant astrocytomaNat CommunJanuary 29, 2025Levi van Hijfte, Marjolein Geurts, Iris de Heer, Santoesha A. Ghisai, Hayri E. Balcioglu, Youri Hoogstrate, Wies R. Vallentgoed, Rania Head, Rosa Luning, Thierry van den Bosch, et al.ABD, Akoya Biosciences, OP-001001) for 15 min at RT. Antigen retrieval using AR6 (Akoya Biosciences, #AR600250ML) at 90 °C for 15 min was followed by incubation in ABD for 10 min at RT, anti-CD20 (1/1000 in ABD; Perklin

(11) Intestinal Akkermansia muciniphila complements the efficacy of PD1 therapy in MAFLD-related hepatocellular carcinomaCell Reports MedicineJanuary 21, 2025Xue Qian Wu, Fan Ying, Katherine Po Sin Chung, Carmen Oi Ning Leung, Rainbow Wing Hei Leung, Karl Kam Hei So, Martina Mang Leng Lei, Wing Ki Chau, Man Tong, Jun Yu, et al.50x Envision FLEX Target Retrieval Buffer (pH9.0, K8004, Dako) for TLR2, CD11b, CD4, CD8, PD1 (pH6.0, AR600250ML, Akoya BioSciences) for 15 min. Endogenous peroxidase activities were quenched using 3% hydrogen peroxide

(12) CHIT1 at diagnosis predicts faster disability progression and reflects early microglial activation in multiple sclerosisNature CommunicationsJune 12, 2024Jarne Beliën, Stijn Swinnen, Robbe D’hondt, Laia Verdú Verdú de Juan, Nina Dedoncker, Patrick Matthys, Jan Bauer, Celine Vens, Sinéad Moylett, Bénédicte Duboisrinsed with TBST, fixed with 4% PFA (10 min) and steamed with Antigen Retrieval buffer (AR6, Akoya, AR600250ML, 30 min). From here, the above-mentioned steps were repeated for each primary antibody. Finally, we

(13) Therapeutic Modulation of Arginase with nor-NOHA Alters Immune Responses in Experimental Mouse Models of Pulmonary Tuberculosis including in the Setting of Human Immunodeficiency Virus (HIV) Co-InfectionTropical medicine and infectious diseaseJune 6, 2024Chauhan S, Nusbaum RJ, Huante MB, Holloway AJ, Endsley MA, Gelman BB, Lisinicchia JG, Endsley JJ.Enzymatic and heat mediated antigen retrieval was performed using AR6 antigen retrieval buffer (Cat# AR600250ML, Akoya Biosciences, Hopkinton, MA, USA) in (3 × 5 min) EZ-Retriever microwave cycles at 95 °C. Blocking

(14) Chemokine ligand 14 correlates with immune cell infiltration in the gastric cancer microenvironment in predicting unfavorable prognosisFrontiers in PharmacologyJune 3, 2024Bing Lu, Jiawen Shi, Tong Cheng, Congshuo Wang, Manyu Xu, Pingping Sun, Xiaojing Zhang, Lei Yang, Peng Li, Han Wu, et al.room temperature for 10 min with 10% formalin. Then, the sections were microwave-cooked in AR6 buffer (AR600250ML, Akoya Biosciences, United States) to repair antigens, and then, they were placed in an antibody-blocking

(15) CD200 is overexpressed in the pancreatic tumor microenvironment and predictive of overall survivalCancer immunology, immunotherapy : CIIApril 15, 2024Wedig J, Jasani S, Mukherjee D, Lathrop H, Matreja P, Pfau T, D'Alesio L, Guenther A, Fenn L, Kaiser M, et al.min. Slides were stained with opal 780 for 1 h. Antigen-Retrieval was accomplished using AR6 (AKOYA, AR600250ML) and AR9 (AKOYA, AR900250ML) buffers followed by microwaving for 45 s at 100% power and 15 min at 20%

(16) Multi-omic profiling of follicular lymphoma reveals changes in tissue architecture and enhanced stromal remodeling in high-risk patientsCancer cellMarch 11, 2024Radtke AJ, Postovalova E, Varlamova A, Bagaev A, Sorokina M, Kudryashova O, Meerson M, Polyakova M, Galkin I, Svekolkin V, et al.Chemicals,Cat#93-0397 Chrome Alum Gelatin,Newcomer Supply,Cat#1033A AR6 buffer 10X,Akoya Biosciences,Cat#AR600250ML Bond™ Epitope Retrieval 1-1L,Leica Biosystems,Cat#AR9961 Bond™ Epitope Retrieval 2-1L,Leica Biosystems,Cat#AR9640 "Wash

(17) Lineage plasticity enables low-ER luminal tumors to evolve and gain basal-like traitsBreast cancer research : BCRMarch 1, 2023Mohamed GA, Mahmood S, Ognjenovic NB, Lee MK, Wilkins OM, Christensen BC, Muller KE, Pattabiraman DR.min in a microwave at 100% power, followed by 15 min at 20% power in AR6 Buffer (Akoya Biosciences, AR600250ML) to affix Opal to target sites and remove primary and secondary antibodies. This process is repeated

(18) Ewing Sarcoma and Osteosarcoma Have Distinct Immune Signatures and Intercellular Communication NetworksClinical cancer research : an official journal of the American Association for Cancer ResearchNovember 14, 2022Cillo AR, Mukherjee E, Bailey NG, Onkar S, Daley J, Salgado C, Li X, Liu D, Ranganathan S, Burgess M, et al.mounted formalin-fixed paraffin embedded tumor tissue was deparaffinized. AR6 (Akoya Biosciences, cat# AR600250ML) or AR9 (Akoya Biosciences AR900250ML) were utilized for heat induced antigen retrieval in serial cycles

(19) Checkpoint Blockade-Induced Dermatitis and Colitis Are Dominated by Tissue-Resident Memory T Cells and Th1/Tc1 CytokinesCancer immunology researchOctober 4, 2022Reschke R, Shapiro JW, Yu J, Rouhani SJ, Olson DJ, Zha Y, Gajewski TF.retrieval was performed using Akoya Bioscience antigen retrieval buffer with low pH (pH 6; catalog no. AR600250ML) or high pH (pH 9; catalog no. AR900250ML). Each section underwent five rounds of antibody staining,

(20) Meflin-positive cancer-associated fibroblasts enhance tumor response to immune checkpoint blockadeLife science allianceJune 1, 2022Miyai Y, Sugiyama D, Hase T, Asai N, Taki T, Nishida K, Fukui T, Chen-Yoshikawa TF, Kobayashi H, Mii S, et al.instructions. Briefly, FFPE sections were deparaffinized, followed by antigen retrieval in AR6 or AR9 (AR600250ML, AR900250ML; Akoya Biosciences) for 15 min at 98°C. After blocking with Antibody Diluent/Blocking (ARD1001EA;

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AR6 buffer, 10X, 250 mL from Akoya Biosciences, a Quanterix company

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Akoya Biosciences, a Quanterix company

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AR6 buffer, 10X, 250 mL from Akoya Biosciences, a Quanterix company

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