AR9 buffer, 10X, 250 mL from Akoya Biosciences, a Quanterix company

Product Specs
ItemAR9 buffer, 10X, 250 mL
CompanyAkoya Biosciences, a Quanterix company
Price $111.08Supplier Page View Company Product Page
Catalog NumberAR900250ML
Quantity1
ApplicationsMultiplex IHC
Typebuffer

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Akoya Biosciences, a Quanterix company

900 Middlesex Turnpike

Building 1

Billerica, MA 01821

United States

Phone: +1 (617) 765-7943

Email: sales@quanterix.com

Company Profile

Website: quanterix.com

(1) Differentiation grade is highly concordant between matched primary and metastatic colorectal cancerClin Exp MetastasisOctober 17, 2025Camilla M. Reehorst, Jennifer K. Mooi, Charles J. Uy, Kristen A. Needham, Sarah Ellis, Ian Y. Luk, Laura J. Jenkins, Rebecca Nightingale, Fiona Chionh, Andreas Behren, et al.retrieval (HIER) by submerging the slides in pH9 or pH6 antigen retrieval (AR) buffer (Akoya Biosciences, AR900250ML and AR600250ML, Akoya Biosciences) and microwaving at 98 °C (20% power) for 15 min. Slides were washed

(2) Latent plasticity of the human pancreas across development, health, and diseasebioRxivOctober 7, 2025Elisabetta Mereu, Diego Balboa, Johannes Liebig, Aitor Gonzalez-Herrero, Anna Martinez-Casals, Mariya Mardamshina, Fanny Mollandin, Felix Schicktanz, Luca Tosti, Valerie Vandenbempt, et al.Heat-Induced Epitope Retrieval (HIER) treatment, using pH 6 EDTA buffer (Akoya Biosciences, Cat. No. AR900250ML) in a pressure cooker (Bio SB TintoRetriever, Cat. No. BSB-7087) at a temperature of 114–121 °C, high

(3) Flexible and robust cell-type annotation for highly multiplexed tissue imagesCell SystemsSeptember 17, 2025Huangqingbo Sun, Shiqiu Yu, Anna Martinez Casals, Anna Bäckström, Yuxin Lu, Cecilia Lindskog, Matthew Ruffalo, Emma Lundberg, Robert F. Murphyheat-induced epitope retrieval (HIER) treatment using pH 9 EDTA buffer (Akoya Biosciences, Cat No. AR900250ML) in a pressure cooker (Bio SB TintoRetriever, Cat No. BSB-7087) at 114–121 °C for 20 minutes. Once the

(4) SARC028 samples reveal an interplay between TGFβ, interferon signaling and low HLA class I expression as contributors to Ewing sarcoma checkpoint blockade resistanceClinical Cancer ResearchSeptember 2, 2025Jessica D. Daley, Elina Mukherjee, David Ferraro, Shanthi Bhaskar, Anthony Green, Ernest M. Meyer, Hussain Tawbi, Melissa Burgess, Tullia C. Bruno, Anthony R. Cillo, et al.(1:250, Cell Signaling Technology, catalog No. 12653, RRID:AB_2797979), and AR9 (Akoya Biosciences AR900250ML) was used for heat-induced antigen retrieval in serial cycles to stain tissues for CD4 (prediluted,

(5) Integrative multi-omics reveals a regulatory and exhausted T-cell landscape in CLL and identifies galectin-9 as an immunotherapy targetNat CommunAugust 7, 2025L. Llaó-Cid, Jkl Wong, I. Fernandez Botana, Y. Paul, M. Wierz, L-M Pilger, A. Floerchinger, C L Tan, S. Gonder, G. Pagano, et al.water. Slides were then cooked in a steam cooker with antigen retrieval buffer pH9 (Akoya Biosciences, AR900250ML) at 100 °C for 25 min. Slides were allowed to cool at room temperature and washed in tris-buffered saline

(6) Enhanced IL36RN Expression and Its Association With Immune Microenvironment Predicts Poor Prognosis in Gastric CancerCancer medicineMay 1, 2025Zhang X, Jiang S, Yin H, Zhang H, Yang L, Sun P, Kuai X, Chen C, Huang J.conducted according to established protocols [21]. Antigen retrieval was performed using AR6 buffer (AR900250ML, AKOYA, Boston, USA) and AR9 buffer (AR600250ML, AKOYA, Boston, USA). Sequential rounds of staining

(7) Quantitatively defined stromal B cell aggregates are associated with response to checkpoint inhibitors in unresectable melanomaCell ReportsApril 22, 2025James W. Smithy, Xiyu Peng, Fiona D. Ehrich, Andrea P. Moy, Mohammad Yosofvand, Colleen Maher, Nathaniel Aleynick, Rami Vanguri, Mingqiang Zhuang, Jasme Lee, et al.heat-induced stripping steps of the primary/secondary antibody complex were performed using Akoya AR9 buffer (AR900250ML) and Leica Bond ER2 (90% AR9 and 10% ER2) at 100°C for 20 min, 3 × 2 min Bond washing steps preceded

(8) Gemistocytic tumor cells programmed for glial scarring characterize T cell confinement in IDH-mutant astrocytomaNat CommunJanuary 29, 2025Levi van Hijfte, Marjolein Geurts, Iris de Heer, Santoesha A. Ghisai, Hayri E. Balcioglu, Youri Hoogstrate, Wies R. Vallentgoed, Rania Head, Rosa Luning, Thierry van den Bosch, et al.using the Opal™ reagents were performed as follows: Antigen retrieval using AR9 (Akoya Biosciences, #AR900250ML) at 90 °C for 15 min was followed by incubation in antibody diluent (ABD; Akoya Biosciences, #ARD1001EA)

(9) Progressive plasticity during colorectal cancer metastasisNatureJanuary 1, 2025Moorman A, Benitez EK, Cambulli F, Jiang Q, Mahmoud A, Lumish M, Hartner S, Balkaran S, Bermeo J, Asawa S, et al.followed by heat-induced stripping of the primary–secondary antibody complex using Akoya AR9 buffer (AR900250ML) and Leica Bond ER2 (90% AR9 and 10% ER2) at 100 °C for 20 min preceding the next cycle (1 cycle of

(10) CHIT1 at diagnosis predicts faster disability progression and reflects early microglial activation in multiple sclerosisNature CommunicationsJune 12, 2024Jarne Beliën, Stijn Swinnen, Robbe D’hondt, Laia Verdú Verdú de Juan, Nina Dedoncker, Patrick Matthys, Jan Bauer, Celine Vens, Sinéad Moylett, Bénédicte Dubois(PFA, 20 min), rinsed with deionized water and steamed in Antigen Retrieval buffer (pH 9.0, Akoya, AR900250ML) in a household food steamer (Braun, 1 h). The sections were rinsed with TBS-Tween 2.0 (TBST, pH 7.5)

(11) CD200 is overexpressed in the pancreatic tumor microenvironment and predictive of overall survivalCancer immunology, immunotherapy : CIIApril 15, 2024Wedig J, Jasani S, Mukherjee D, Lathrop H, Matreja P, Pfau T, D'Alesio L, Guenther A, Fenn L, Kaiser M, et al.opal 780 for 1 h. Antigen-Retrieval was accomplished using AR6 (AKOYA, AR600250ML) and AR9 (AKOYA, AR900250ML) buffers followed by microwaving for 45 s at 100% power and 15 min at 20% power. After completing all

(12) Stem cell-like T cell depletion in the recurrent head and neck cancer immune microenvironmentOncoImmunologyDecember 31, 2023Linda Chen, Nancy Lee, Reith Sarkar, Nora Katabi, Yanyun Li, Luc Morris, Richard Wong, Eric Sherman, Jorge S. Reis-Filho, Travis Hollmann, et al.staining cycle, a heat induced stripping of the primary/secondary antibody complex using Akoya AR9 buffer (AR900250ML) and Leica Bond ER2 (90% AR9 and 10% ER2) at 100°C for 20 min preceding the next cycle with Ki67 requiring

(13) Tissue-specific features of the T cell repertoire after allogeneic hematopoietic cell transplantation in human and mouseScience translational medicineJuly 26, 2023DeWolf S, Elhanati Y, Nichols K, Waters NR, Nguyen CL, Slingerland JB, Rodriguez N, Lyudovyk O, Giardina PA, Kousa AI, et al.heat-induced stripping of the primary/secondary antibody complex was performed using Akoya AR9 buffer (AR900250ML) and Leica Bond ER2 (90% ER2 and 10% AR9) at 100°C for 20 minutes preceding the next cycle. After 6

(14) Ewing Sarcoma and Osteosarcoma Have Distinct Immune Signatures and Intercellular Communication NetworksClinical cancer research : an official journal of the American Association for Cancer ResearchNovember 14, 2022Cillo AR, Mukherjee E, Bailey NG, Onkar S, Daley J, Salgado C, Li X, Liu D, Ranganathan S, Burgess M, et al.tumor tissue was deparaffinized. AR6 (Akoya Biosciences, cat# AR600250ML) or AR9 (Akoya Biosciences AR900250ML) were utilized for heat induced antigen retrieval in serial cycles in order to stain tissues for CD4

(15) Checkpoint Blockade-Induced Dermatitis and Colitis Are Dominated by Tissue-Resident Memory T Cells and Th1/Tc1 CytokinesCancer immunology researchOctober 4, 2022Reschke R, Shapiro JW, Yu J, Rouhani SJ, Olson DJ, Zha Y, Gajewski TF.Bioscience antigen retrieval buffer with low pH (pH 6; catalog no. AR600250ML) or high pH (pH 9; catalog no. AR900250ML). Each section underwent five rounds of antibody staining, each round consisting of blocking (Akoya

(16) Tumor MHC Class I Expression Associates with Intralesional Interleukin-2 Response in MelanomaCancer Immunology ResearchMarch 1, 2022Maryam Pourmaleki et al.followed by heat-induced stripping of the primary/secondary antibody complex using Akoya AR9 buffer (cat. #AR900250ML) and Leica Bond ER2 (90% ER2 and 10% AR9) at 100°C for 20 minutes preceding the next cycle. After three

(17) Sting Is Commonly and Differentially Expressed in T- and Nk-Cell but Not B-Cell Non-Hodgkin LymphomasCancersFebruary 24, 2022Xagoraris I, Farrajota Neves da Silva P, Kokaraki G, Stathopoulou K, Wahlin B, Österborg A, Herold N, Ng SB, Medeiros LJ, Drakos E, et al.tissue sections and antigen retrieval using a cooking steamer and antigen retrieval solution (cat. no. AR900250ML, Akoya Biosciences, Marlborough, MA, USA), the sections were incubated with the primary antibodies overnight

(18) Spatial UMAP and Image Cytometry for Topographic Immuno-oncology Biomarker DiscoveryCancer Immunology ResearchNovember 1, 2021Nicolas A. Giraldo et al.through microwave pretreatment with AR9 followed by AR6 (Akoya Biosciences, Marlborough, MA, cat. #AR900250ML and #AR600125ML). The first primary antibody (“Position 1”) was then applied. Opal polymer HRP Ms+Rb

(19) Meflin defines mesenchymal stem cells and/or their early progenitors with multilineage differentiation capacityGenes to cells : devoted to molecular & cellular mechanismsJuly 1, 2021Hara A, Kato K, Ishihara T, Kobayashi H, Asai N, Mii S, Shiraki Y, Miyai Y, Ando R, Mizutani Y, et al.manufacturer's instruction. Briefly, FFPE sections were deparaffinized, followed by antigen retrieval in AR9 (AR900250ML, Akoya Biosciences) for 15 min at 98°C. After blocking with Antibody Diluent/Blocking (ARD1001EA, Akoya Biosciences

(20) Meflin-positive cancer-associated fibroblasts enhance tumor response to immune checkpoint blockadeLife science allianceJanuary 1, 0001"Miyai Y, Sugiyama D, Hase T, Asai N, Taki T, Nishida K, Fukui T, Chen-Yoshikawa TF, Kobayashi H, Mii S, et al."Briefly, FFPE sections were deparaffinized, followed by antigen retrieval in AR6 or AR9 (AR600250ML, AR900250ML; Akoya Biosciences) for 15 min at 98°C. After blocking with Antibody Diluent/Blocking (ARD1001EA; Akoya Biosciences

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AR9 buffer, 10X, 250 mL from Akoya Biosciences, a Quanterix company

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Akoya Biosciences, a Quanterix company

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AR9 buffer, 10X, 250 mL from Akoya Biosciences, a Quanterix company

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