1X OPAL ANTI-MS + RB HRP (formerly Opal Polymer HRP Ms + Rb, 1X, 50 mL) from Akoya Biosciences, a Quanterix company

Product Specs
Item1X OPAL ANTI-MS + RB HRP (formerly Opal Polymer HRP Ms + Rb, 1X, 50 mL)
CompanyAkoya Biosciences, a Quanterix company
Price $586.58Supplier Page View Company Product Page
Catalog NumberARH1001EA
Quantity1
ApplicationsMultiplex IHC
ReactivityAny (including Human)

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Akoya Biosciences, a Quanterix company

900 Middlesex Turnpike

Building 1

Billerica, MA 01821

United States

Phone: +1 (617) 765-7943

Email: sales@quanterix.com

Company Profile

Website: quanterix.com

(1) Persistent glycolysis defines pathological foreign body-associated inflammation to polymeric implantsSci AdvDecember 19, 2025Christian Rempe, Neal Callaghan, Lauren Fong-Hollohan, Sarah Nersesian, Zachary Froom, Kyle Medd, Ibrahim Ahmed, Tobias Karakach, Jeanette E. Boudreau, Michael Bezuhly, et al.phosphate-buffered saline (DPBS) (Corning, 20-031-CV), 1X Opal Polymer HRP MS + RB (Akoya Biosciences, ARH1001EA), 1X RPMI 1640 + l-glutamine (300 mg liter−1) (Corning, 10-040-CV), 1X Plus Manual Amplification Diluent

(2) Mast Cell Association with the Microenvironment of a Phosphaturic Mesenchymal Tumour Secreting Fibroblast Growth Factor 23Medical SciencesSeptember 16, 2025Andrey Kostin, Alexei Lyundup, Alexander Alekhnovich, Aleksandra Prikhodko, Olga Patsap, Sofia Gronskaia, Zhanna Belaya, Olga Lesnyak, Galina Melnichenko, Natalia Mokrysheva, et al.FP1609,"Akoya Biosciences, Marlborough, MA, USA",ready-to-use,w/o Opal Polymer HRP Ms + Rb Akoya Biosciences ARH1001EA,"Akoya Biosciences, Marlborough, MA, USA",ready-to-use,HRP Antibody Diluent/Block buffer Akoya Biosciences

(3) Renal prognostic value of serum monoclonal immunoglobulin in cryoglobulinemic glomerulonephritisFrontiers in ImmunologyJuly 29, 2025Lei Ma, Yuanyuan Xia, Yun Fan, Dan Zhou, Xinchen Yao, Yongzhong Zhong, Fan Yang, Feng Xu, Shaoshan Liang, Yujie Wang, et al.hour. After incubation with a universal secondary antibody for mouse and rabbit (Akoya Biosciences, ARH1001EA) at room temperature for 30 minutes, corresponding dyes were applied according to the primary antibody

(4) HMGA2 and protein leucine methylation drive pancreatic cancer lineage plasticityNat CommunMay 26, 2025Stephanie Dobersch, Naomi Yamamoto, Aidan Schutter, Sarah M. Cavender, Tess M. Robertson, Nithya Kartha, Annie N. Samraj, Ben Doron, Lisa A. Poole, Cynthia L. Wladyka, et al.HRP was used for all secondary applications, either 1X Opal Anti-Ms + Rb HRP (Akoya Biosciences, Cat #ARH1001EA) or PowerVision Poly-HRP anti-mouse IgG (Leica Biosystems, Cat #PV6114). Primary and secondary antibodies

(5) Harnessing nutrient scarcity for enhanced CAR-T-cell potency and safety in solid tumorsCell Mol ImmunolMay 8, 2025Enzo Manchon, Nell Hirt, Benjamin Versier, Aravindhan Soundiramourty, Ludmila Juricek, Celeste Lebbe, Maxime Battistella, Yves Christen, Jacques Mallet, Dominique Charron, et al.incubated with an anti-CD3 antibody (ab11089, Abcam), incubated with a secondary antibody against Akoya (ARH1001EA, Akoya), and then incubated with Opal 520 (1:250) (OP001001, Akoya). The second staining cycle included

(6) LINE-1 ORF1p Mimics Viral Innate Immune Evasion Mechanisms in Pancreatic Ductal AdenocarcinomaCancer DiscoveryMay 2, 2025Eunae You, Bidish K. Patel, Alexandra S. Rojas, Siyu Sun, Patrick Danaher, Natalie I. Ho, Ildiko E. Phillips, Michael J. Raabe, Yuhui Song, Katherine H. Xu, et al.room temperature. The slides were then washed, and the Opal anti-Ms + Rb HRP (Akoya Biosciences, cat. #ARH1001EA) was applied for 30 minutes at room temperature. The slides were washed again, and the Opal 620 dye

(7) Association of peripheral monocytic myeloid-derived suppressor cells with molecular subtypes in single-center endometrial cancer patients receiving carboplatin + paclitaxel/avelumab (MITO-END3 trial)Cancer Immunol ImmunotherApril 17, 2025C. D'Alterio, G. Rea, M. Napolitano, E. Coppola, A. Spina, D. Russo, R. Azzaro, C. Mignogna, G. Scognamiglio, D. Califano, et al.followed by secondary: HRP-conjugated AKOYA biosciences Opal Polymer HRP Ms + Rb (Akoya Biosciences Cat# ARH1001EA, RRID:AB_2890927) and Opal™ 520 (1:250, PerkinElmer—Akoya Biosciences Cat# NEL811001KT, RRID:AB_3665660).

(8) Multiplex immunofluorescence assessment of macrophages and IL-23R in inflammatory and malignant diseases of the oral mucosa: a pilot studyFront ImmunolApril 14, 2025Leah Trumet, Bettina Grötsch, Abbas Agaimy, Kerstin Galler, Carol Geppert, Linus Winter, Jutta Ries, Marco Kesting, Manuel Webermin. For signal amplification, 1X Opal Anti-Ms + Rb HRP (Akoya Biosciences, Marlborough, MA, USA; REF: ARH1001EA) was used as a secondary antibody and Opal polymer and applied for 10 min. The Opal Anti-MS polymer

(9) Artificial intelligence-based tissue segmentation and cell identification in multiplex-stained histological endometriosis sectionsHuman ReproductionMarch 1, 2025Scott E Korman, Guus Vissers, Mark A J Gorris, Kiek Verrijp, Wouter P R Verdurmen, Michiel Simons, Sebastien Taurin, Mai Sater, Annemiek W Nap, Roland BrockThe sections were then incubated with a secondary antibody (Akoya BioSciences, Opal Polymer HRP, cat# ARH1001EA, USA) for 30 min. After rinsing for 10 min, 100 µl of the Opal 650 fluorophore (Akoya Biosciences, cat#

(10) Exploring the characteristics of immortalized human ovarian surface epithelial cell linesHeliyonFebruary 28, 2025Ha-Yeon Shin, Wookyeom Yang, Jue Young Kim, Ha Kyun Chang, Jongman Yoo, Woo Hee Choi, Gwan Hee Han, Hanbyoul Cho, Jae-Hoon KimDiluent/block buffer. Next, after washing three times with TBS-T, a solution of OPAL polymer HRP Ms + Rb (ARH1001EA, AKOYA, Marlborough, MA, USA) was dropped to the cell block and treated for 10 min at room temperature.

(11) The ubiquitin ligase Pellino1 targets STAT3 to regulate macrophage-mediated inflammation and tumor developmentNature communicationsFebruary 1, 2025Hwang S, Park J, Koo SY, Lee SY, Jo Y, Ryu D, Go H, Lee CW.Opal 7 Immunology Discovery Kit, Akoya Biosciences), CK (1:1000, NCL-L-AE1/AE3, Leica Biosystems), and secondary antibodies (ARH1001EA, Akoya Biosciences) for 1 h. TSA visualization was performed using Opal fluorophores [Opal 520 (Pellino1),

(12) Gemistocytic tumor cells programmed for glial scarring characterize T cell confinement in IDH-mutant astrocytomaNat CommunJanuary 29, 2025Levi van Hijfte, Marjolein Geurts, Iris de Heer, Santoesha A. Ghisai, Hayri E. Balcioglu, Youri Hoogstrate, Wies R. Vallentgoed, Rania Head, Rosa Luning, Thierry van den Bosch, et al.#MRQ-42) staining for 30 min at RT, incubation with HRP polymer Ms + RB (1/500 in ABD; Akoya Biosciences, #ARH1001EA) for 10 min at RT and finally incubation with opal 620 (1/100 in ABD; Akoya Biosciences, #OP-001004)

(13) Intestinal Akkermansia muciniphila complements the efficacy of PD1 therapy in MAFLD-related hepatocellular carcinomaCell Reports MedicineJanuary 21, 2025Xue Qian Wu, Fan Ying, Katherine Po Sin Chung, Carmen Oi Ning Leung, Rainbow Wing Hei Leung, Karl Kam Hei So, Martina Mang Leng Lei, Wing Ki Chau, Man Tong, Jun Yu, et al.Cell Signaling Technology. The sections were then washed thoroughly and incubated with Opal polymer HRP Mouse+Rabbit (ARH1001EA, Akoya Biosciences) for 30 min at room temperature. Followed by a brief wash with 1×TBST, Opal fluorophore

(14) Single‑cell transcriptomic analysis revealed the tumor‑associated microenvironment of papillary thyroid carcinoma with metastasisOncology LettersJanuary 7, 2025Ni Zhang, Qingbin Liu, Qian Wang, Xiuli Liu, Suya Zhang, Xinchen Tian, Long Li, Shuanglong Wang, Bin Lv, Shulong Jiangcorresponding secondary horseradish peroxidase-conjugated antibody against mouse or rabbit immunoglobulins (ARH1001EA; Akoya Biosciences). The slides were then incubated in different Opal fluorophores (1:100) diluted in

(15) A five-plex Hepatic Oncochip reveals EMT triplet correlated with BAP31 in liver cancerFront Cell Dev BiolJanuary 6, 2025Youjia Kong, Tengfei Zhuang, Xvshen Ding, Sirui Cai, Weijie Ding, Xiaoxiao Zhang, Yubo Sun, Bingquan Zhou, Yuanjie Sun, Shuya Yang, et al.66219-1-IG)—followed by incubation with HRP-conjugated secondary antibodies (Akoya; Opal Polymer HRP Ms + Rb; ARH1001EA) to detect specific molecular markers. Afterwards, during each round of staining, the slides are incubated

(16) Mouse Models for Pancreatic Ductal Adenocarcinoma are Affected by the cre-driver Used to Promote KRASG12D ActivationCellular and Molecular Gastroenterology and HepatologyJanuary 1, 2025Fatemeh Mousavi, Joyce Thompson, Justine Lau, Nur Renollet, Mickenzie B. Martin, Jake McGue, Oneeb Hassan, Timothy Frankel, Parisa Shooshtari, Christopher L. Pin, et al.triplicate, tissues were incubated with secondary antibody, Opal Anti-Ms + Rb HRP (Akoya Biosciences, ARH1001EA), before conjugating with Opal fluorophores. Following incubation, tissue samples were rinsed in deionized

(17) Progressive plasticity during colorectal cancer metastasisNatureJanuary 1, 2025Moorman A, Benitez EK, Cambulli F, Jiang Q, Mahmoud A, Lumish M, Hartner S, Balkaran S, Bermeo J, Asawa S, et al.performed using a secondary horseradish peroxidase (HRP)-conjugated polymer (Akoya Opal polymer HRP Ms + Rb ARH1001EA; 1:5, 10 min incubation). Detection of all of the other primary antibodies was performed using goat

(18) Cancer-specific innate and adaptive immune rewiring drives resistance to PD-1 blockade in classic Hodgkin lymphomaNature CommunicationsDecember 30, 2024Julia Paczkowska, Ming Tang, Kyle T. Wright, Li Song, Kelsey Luu, Vignesh Shanmugam, Emma L. Welsh, Jason L. Weirather, Naomi Besson, Harrison Olszewski, et al.antibodies, including anti-mouse and anti-rabbit Opal Polymer Horseradish Peroxidase (Akoya Biosciences #ARH1001EA), along with Post Primary Block (Leica Biosystems #RE7159) and Polymer (Leica Biosystems #RE7161), were

(19) Pneumococcal pneumonia is driven by increased bacterial turnover due to bacteriocin-mediated intra-strain competitionCommunications biologyDecember 6, 2024Aggarwal SD, Lokken-Toyli KL, Weiser JN.Statens Serum Institut; cat. #16747) for 60 min followed by an anti-rabbit secondary polymer (Akoya ARH1001EA) and HRP-mediated tyramide signal amplification with the 520 Opal® fluorophore (Akoya FP1487001KT).

(20) Proteogenomic analysis dissects early-onset breast cancer patients with prognostic relevanceExperimental & molecular medicineNovember 1, 2024Yoon KA, Kim Y, Jung SY, Ryu JS, Kim KH, Lee EG, Chae H, Kwon Y, Kim J, Park JB, et al.PD-1 (ab137132; Abcam; dilution 1:500). Then, the sections were incubated with the Polymer HRP Ms+Rb (ARH1001EA, AKOYA Biosciences) and subsequently treated with an Opal fluorophore (Opal690, Opal570, Opal520, Opal620,

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1X OPAL ANTI-MS + RB HRP (formerly Opal Polymer HRP Ms + Rb, 1X, 50 mL) from Akoya Biosciences, a Quanterix company

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Akoya Biosciences, a Quanterix company

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1X OPAL ANTI-MS + RB HRP (formerly Opal Polymer HRP Ms + Rb, 1X, 50 mL) from Akoya Biosciences, a Quanterix company

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