Western-Ready™ Protein Sample Loading Buffer (5X) from BioLegend

Product Specs
ItemWestern-Ready™ Protein Sample Loading Buffer (5X)
CompanyBioLegend
Price $47.00Supplier Page View Company Product Page
Catalog Number426311
Quantity20 mL
ApplicationsWB - Quality tested
Format300 mM Tris-HCl pH 6.8, 10% Sodium dodecyl sulfate, 30% glycerol, 0.02% Bromophenol blue

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) A Conserved Enhancer Locus in Extrachromosomal DNA and Homogeneously Staining Regions Activates MYC Transcription in Group 3 MedulloblastomaCancer ResApril 22, 2026Jake D. Friske, Flore Cuisin, Paloma Guernalec, Hayden A. Malone, Stephanie Nance, Declan Bennett, Steven J. Burden, Ti-Cheng Chang, Hao Shi, Justin S. Williams, et al.concentration of 20 μg per lane in a final volume of 20 μL, containing 4 μL of 5x loading buffer (Biolegend #426311). Samples were adjusted with lysis buffer when needed, heated at 95°C for 5 minutes, immediately cooled

(2) Inherent Lipid Composition Abnormalities in Astrocytes Associated with Late-Onset Alzheimer's Disease (LOAD)CellsMarch 19, 2026Cohen BM, Koh E, Levental KR, Levental I, Sonntag KC.were mixed with Western-Ready Protein Sample Loading Buffer (BioLegend, San Diego, CA, USA, Cat No. 426311) containing 5% beta mercaptoethanol (ß-ME, Sigma-Aldrich, Cat No. M3148) and heated at 60 °C for 10

(3) Temporal and cell-type specific SPAK-NKCC1 disruption following severe TBI in the developing gyrencephalic brainActa Neuropathologica CommunicationsFebruary 24, 2026Alexandra Hochstetler, Ya’el Courtney, Peace Oloko, Benjamin Baskin, Andrew Ding-Su, Tawny Stinson, Elyssa Alber, David Y. Chung, Declan McGuone, Robin Haynes, et al.(ThermoScientific #23250). 20 µg of the sample was reduced with 5X Laemmli Sample Buffer (BioLegend #426311) supplemented with B-mercaptoethanol (5%) and diluted with double distilled water to an equivalent volume.

(4) A GDF-15–GFRAL axis controls autoimmune T cell responses during neuroinflammationNature ImmunologyJanuary 15, 2026Jana K. Sonner, Audrey Kahn, Lars Binkle-Ladisch, Jan Broder Engler, Beatrice Haack, Christina Zeiler, Lisa Unger, Simone Bauer, Felix Fischbach, Giovanni Almanzar, et al.manufacturer’s protocol. Samples were prepared using Western-Ready Protein Sample Loading Buffer (5×; BioLegend, 426311) and boiled at 95 °C for 10 min. A total of 10 µg of protein was loaded onto 8–12% gradient or 10% non-gradient

(5) Mass Cytometry-Based Approach for the Investigation of Stimulator of Interferon Genes PathwayEuropean journal of immunologyDecember 1, 2025Reversat A, Kennedy PT, Georghiou A, Slupsky JR, Dahal LN.electrophoresis, lysates were thawed, diluted in Western‐Ready Protein Sample Loading Buffer (5X, BioLegend, #426311) or 4X Laemmli Sample Buffer (Bio‐Rad, #1610747), and incubated at 95°C for 5–10 min to denature proteins.

(6) TERT PfeRNA regulates telomere length during cellular senescence of normal human bronchial epithelial cellsSci RepJuly 1, 2025Suguru Yamauchi, Kaitlyn Ecoff, Andrei Gurau, Kristen P. Rodgers, Malcolm V. Brock, Yuping Meito that of IP described above. Proteins were denatured using protein loading buffer (BioLegend, Cat# 426311), boiled for 10 min, separated in 10% SDS-polyacrylamide electrophoresis gel, and transferred to nitrocellulose

(7) Biocompatibility Study of Purified and Low-Temperature-Sterilized Injectable Collagen for Soft Tissue Repair: Intramuscular Implantation in RatsGelsSeptember 26, 2024Tae-Hoon Koo, Jason K. Lee, Shawn P. Grogan, Ho Jong Ra, Darryl D. D’Limadiluted to 1 mg/mL with deionized (DI) water. The diluted collagen was mixed with 5× loading buffer (#426311, BioLegend®, Seoul, Republic of Korea) and boiled at 95 °C for 5 min. The samples were separated by

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Western-Ready™ Protein Sample Loading Buffer (5X) from BioLegend

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Western-Ready™ Protein Sample Loading Buffer (5X) from BioLegend

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