ELISA substrate: TMB for HRP from Mabtech AB

This high sensitivity TMB substrate is suitable for ELISA assays with horseradish peroxidase (HRP). The substrate is supplied as a ready-to-use solution and produces a soluble blue product. Addition of an acid, e.g. sulfuric acid, stops the enzymatic reaction, which changes the color from blue to yellow. The absorbance of the stopped reaction is measured at 450 nm