Apotracker(TM) Green from BioLegend

Product Specs
ItemApotracker(TM) Green
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number427403
Quantity200 tests
ApplicationsFlow Cytometry (FC), Live cell imaging
FormatThis product consist of dry-down Apotracker™ Green and DMSO reconstitution solution
SpeciesHuman, Mouse

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) αβ-T cell receptor transduction gives superior mitochondrial function to γδ-T cells with promising persistenceiScienceOctober 20, 2023Mikiya Ishihara, Hiroshi Miwa, Hiroshi Fujiwara, Yasushi Akahori, Takuma Kato, Yoshimasa Tanaka, Isao Tawara, Hiroshi ShikuLive or dead fixable dead cell staining kit NIR fluorescence,AAT Bioquest,Cat#22605 CytoTell Red 650,AAT Bioquest,Cat#22255 Apotracker Green,Biolegend,Cat#427402 2-deoxy-D-glucose,Sigma–Aldrich,Cat#D8375 Etomoxir,Cayman,Cat#11969 CB-839,Cayman,Cat#22038 Oligomycin,Abcam,Cat#141829 MitoTracker

(2) PSGL-1 attenuates early TCR signaling to suppress CD8(+) T cell progenitor differentiation and elicit terminal CD8(+) T cell exhaustionCell reportsMay 30, 2023Hope JL, Otero DC, Bae EA, Stairiker CJ, Palete AB, Faso HA, Lin M, Henriquez ML, Roy S, Seo H, et al.AB_2616717 APC/Fire™ 750 anti-mouse CD38,BioLegend,Cat# 102738; RRID: AB_2876402 Apotracker™ Green,BioLegend,Cat# 427403 Brilliant Violet 421™ anti-human CD38,BioLegend,Cat# 303526; RRID: AB_10900230 Brilliant Violet 605™

(3) Modeling breast cancer proliferation, drug synergies, and alternating therapiesiScienceMay 19, 2023Wei He, Diane M. Demas, Ayesha N. Shajahan-Haq, William T. BaumannInhibitor Cocktail Tablets,Roche Applied Science,Cat#11836153001 Apotracker,"BioLegend, San Diego, CA",Cat#427402 propidium iodide,"Thermofisher Scientific Waltham, MA",Cat#P1304MP ,, Critical commercial assays,Critical

(4) Circadian regulator CLOCK promotes tumor angiogenesis in glioblastomaCell reportsFebruary 28, 2023Pang L, Dunterman M, Xuan W, Gonzalez A, Lin Y, Hsu WH, Khan F, Hagan RS, Muller WA, Heimberger AB, et al.apoptosis of CT2A cells with or without POSTN knockdown was evaluated using Apotracker Green (BioLegend, #427402).62 Briefly, 105 cells were stained with Apotracker (1:10 dilution) and then 5 μL propidium iodide (PI)

(5) Osteopontin associates with brain T(RM)-cell transcriptome and compartmentalization in donors with and without multiple sclerosisiScienceJanuary 20, 2023Hsiao CC, Engelenburg HJ, Jongejan A, Zhu J, Zhang B, Mingueneau M, Moerland PD, Huitinga I, Smolders J, Hamann J.AB_2833255 Anti-human TNF–Alexa Fluor 700,BD Biosciences,Cat#557996RRID: AB_396978 Apotracker Green,BioLegend,Cat#427402 Donkey-anti-goat–Alexa Fluor 488,Jackson ImmunoResearch,Cat#705-545-003RRID: AB_2340428 Donkey-anti-rabbit–Cy3,Jackson

(6) LY6D marks pre-existing resistant basosquamous tumor subpopulationsNature communicationsDecember 6, 2022Haensel D, Gaddam S, Li NY, Gonzalez F, Patel T, Cloutier JM, Sarin KY, Tang JY, Rieger KE, Aasi SZ, et al.ApotrackerTM experiments, we used the ApotrackerTM Green reagent as per manufacturer instructions (BioLegend; 427402). FlowJo 10.6 was used for data analysis. scRNA-Seq library preparation and sequencing As per the

(7) Fibrotic Lung Disease Alters Neutrophil Trafficking and Promotes Neutrophil Elastase and Extracellular Trap ReleaseImmunoHorizonsDecember 1, 2022Helen I. Warheit-Niemi, Gabrielle P. Huizinga, Summer J. Edwards, Yizhou Wang, Susan K. Murray, David N. O’Dwyer, Bethany B. MooreCD62L-BV421 (catalog no.104435), CXCR4-PE/Dazzle (catalog no. 146513), Apotracker Green (catalog no. 427401) (BioLegend, San Diego, CA). Cells were incubated with Abs in the dark for 30 min on ice, after which

(8) Alum-anchored intratumoral retention improves the tolerability and antitumor efficacy of type I interferon therapiesProceedings of the National Academy of SciencesSeptember 6, 2022Emi A. Lutz, Yash Agarwal, Noor Momin, Sarah C. Cowles, Joseph R. Palmeri, Ellen Duong, Vladlena Hornet, Allison Sheen, Brianna M. Lax, Adrienne M. Rothschilds, et al.(Invitrogen). Some tumors were stained with Zombie NIR (BioLegend 423105), Apotracker Green (BioLegend 427402), BUV395-CD45 (BD Biosciences 30-F11), AF647-TA99 (labeled in-house), PE/Cy7-MHC-I (BioLegend 28-8-6),

(9) An autoimmune stem-like CD8 T cell population drives type 1 diabetesNatureFebruary 3, 2022Sofia V. Gearty et al.measured by incubating cells with 800 nM Apotracker Detection Reagent (Biolegend; catalogue (cat.) no. 427403) for 15 min at room temperature. Adoptive T cell transfer For transfer into first NOD/SCID hosts,

(10) Lymphatic PD-L1 Expression Restricts Tumor-Specific CD8⁺ T-cell ResponsesCancer researchAugust 1, 2021Cousin N, Cap S, Dihr M, Tacconi C, Detmar M, Dieterich LC.Zombie-NIR. T-cell and myeloid responses were examined using Zombie-Aqua, Apotracker-Green (BioLegend 427401, 1:200), AnnexinV-APC (BioLegend 640932), CD45-PacificBlue (clone 30-F11, BioLegend 103126, RRID:AB_493535,

(11) In Vitro Sensitivity to Venetoclax and Microenvironment Protection in Hairy Cell LeukemiaFrontiers in OncologyJuly 26, 2021Alexia Vereertbrugghen et al.from Biolegend (#121406 and 300908, respectively). Annexin V staining and Apotracker Green (Biolegend #427402) were used to evaluate the percentage of cell death induced by venetoclax. After the incubation with

(12) Expansion of CD10neg neutrophils and CD14+HLA-DRneg/low monocytes driving proinflammatory responses in patients with acute myocardial infarctioneLifeJuly 22, 2021Daniela Fraccarollo, Jonas Neuser, Julian Möller, Christian Riehle, Paolo Galuppo, Johann BauersachsViolet/SYTOX AADvanced Apoptosis Kit (A35135, Invitrogen) and Apotracker Green (Barth et al., 2020) (427402; Biolegend), according to the manufacturer’s protocols. Stained neutrophils were analyzed using a Gallios

(13) Animal-Free Human Whole Blood Sepsis Model to Study Changes in Innate ImmunityFrontiers in immunologyJanuary 1, 2020Messerer DAC, Vidoni L, Erber M, Stratmann AEP, Bauer JM, Braun CK, Hug S, Adler A, Nilsson Ekdahl K, Nilsson B, et al.1:4000, #423114, BioLegend) and apoptotic cells using Apotracker Green (final concentration 200 nM, #427402, BioLegend). The generation of radical oxygen species (ROS) was determined by staining 40 µl heparin

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4/6/2020Excellent Way to Detect Apoptosis, Easier than Annexin V Elisebeth TorrettiThis worked great for detecting FAS agonist-mediated Apoptosis in my assay. I look forward to using it in the future.

Apotracker(TM) Green from BioLegend

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BioLegend

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Apotracker(TM) Green from BioLegend

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