BioLegend
Apotracker™ Green
427402
Info:Supplier Page View Product SpecsView Product
In this assay I treated Jurkat cells with a FAS agonist antibody to induce apoptosis. I then stained with Biolegend's next Apotracker green to test it.
Flow Cytometry
Jurkat cells
20 minutes RT
N/A
Flow cytometer, FITC channel
This apotracker green reagent was able to easily detect the apoptosis induced by FAS. I checked it against a live/dead cell reagent commonly used in my lab(Cell titer glo) and the results of the apotracker green matched with those from the cell titer glo.
I used at 1:50, but protocol suggests use between 1:50-1:400 (depending on cell type)
Quick/easy to use, no special buffers required
Nothing bad
This worked great for detecting FAS agonist-mediated Apoptosis in my assay. I look forward to using it in the future.