SignalStain ® Boost IHC Detection Reagent (AP, Rabbit) from Cell Signaling Technology

Product Specs
ItemSignalStain ® Boost IHC Detection Reagent (AP, Rabbit)
CompanyCell Signaling Technology
Price $390.00 Inquire
Catalog Number18653S
Quantity15 ml
ApplicationsIHC-p
Research AreaRelated Products
Source/Expression SystemHorse
ReactivityRab

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) SHP099-containing multi-targeting hydrogel promotes rapid skin reconstruction through modulating a variety of cellsFront Bioeng BiotechnolApril 7, 2025Zhixiao Liu, Lei Chen, Bingbing Hao, Yijin Hou, Chuan Lv, Yuanjie Zhu, Chaofeng Han(19245, CST) overnight at 4°C. The sections were then incubated with AP-labelled secondary antibodies (18653, CST). After reaction with the substrate (76713, CST), Cald1 or alpha-SMA positive cells were stained

(2) Platelets as key cells in endometriosis patients: Insights from small extracellular vesicles in peritoneal fluid and endometriotic lesions analysisFASEB JDecember 19, 2024Barbara Bortot, Roberta Di Florio, Simona Merighi, Ben Peacock, Rebecca Lees, Francesco Valle, Marco Brucale, Alessandro Mangogna, Giovanni Di Lorenzo, Federico Romano, et al.SignalStain® Boost IHC Detection rabbit, alkaline phosphatase (AP)‐conjugated produced in horse (cod. 18653S, Cell Signaling Technology) and DAB (3,3′‐Diaminobenzidine, Leica Biosystems) or Vulcan Fast Red (BioOptica)

(3) Platelets as key cells in endometriosis patients: Insights from small extracellular vesicles in peritoneal fluid and endometriotic lesions analysisThe FASEB JournalDecember 19, 2024Barbara Bortot, Roberta Di Florio, Simona Merighi, Ben Peacock, Rebecca Lees, Francesco Valle, Marco Brucale, Alessandro Mangogna, Giovanni Di Lorenzo, Federico Romano, et al.SignalStain® Boost IHC Detection rabbit, alkaline phosphatase (AP)‐conjugated produced in horse (cod. 18653S, Cell Signaling Technology) and DAB (3,3′‐Diaminobenzidine, Leica Biosystems) or Vulcan Fast Red (BioOptica)

(4) Interleukin‐6 transcripts up‐regulation in lymph nodes from unicentric and multicentric Castleman diseaseeJHaemDecember 1, 2024Marco Lucioni, Gaia Morello, Caterina Cristinelli, Sara Fraticelli, Giuseppe Neri, Erica Travaglino, Marco Minetto, Francesca Antoci, Paolo Libretti, Marcello Gambacorta, et al.Biosystems). Double IHC staining was performed by applying SignalStainBoost IHC Detection rabbit (cod. #18653, Cell Signaling Technology) alkaline phosphatase‐conjugated produced in horse and Vulcan Fast Red as

(5) UBE2C enhances temozolomide resistance by regulating the expression of p53 to induce aerobic glycolysis in gliomaActa Biochimica et Biophysica SinicaApril 18, 2024Kun Zhou, Dexin Wang, Xiaolin Du, Xia Feng, Xiaoxi Zhu, Cheng WangAbcam) overnight at 4°C. After that, the sections were incubated with an HRP-labeled secondary antibody (18653; CST) for 1 h at room temperature. The expression levels of UBE2C and p53 were scored by the area and

(6) Glomerular spatial transcriptomics of IgA nephropathy according to the presence of mesangial proliferationScientific reportsJanuary 26, 2024Park S, Kang M, Kim YC, Kim DK, Oh KH, Joo KW, Kim YS, Kim HJ, Moon KC, Lee H.saline, 0.1% tween 20 (1× TBST). The secondary antibody used was anti-rabbit AP-conjugated antibody (18653S, Cell Signaling Technology). A substrate mixture was applied to all slides, and the tissues were counterstained

(7) Hydrogel armed with Bmp2 mRNA-enriched exosomes enhances bone regenerationJournal of nanobiotechnologyApril 5, 2023Yang Z, Li X, Gan X, Wei M, Wang C, Yang G, Zhao Y, Zhu Z, Wang Z.Boster) and COL-1a (PB0981, Boster) staining was performed with an anti-rabbit HRP/DAB Detection Kit (18653 s, CST), following the manufacturer’s protocol. The images were photographed under the microscope. Semiquantitative

(8) SARS-CoV-2 infection induces DNA damage, through CHK1 degradation and impaired 53BP1 recruitment, and cellular senescenceNature cell biologyApril 1, 2023Gioia U, Tavella S, Martínez-Orellana P, Cicio G, Colliva A, Ceccon M, Cabrini M, Henriques AC, Fumagalli V, Paldino A, et al.previously reported91. Double IHC staining was conducted by applying SignalStainBoost IHC Detection (#18653; #31926, Cell Signaling) and Vulcan Fast Red as substrate chromogen. For combined ISH and IHC staining,

(9) Impaired activation of plasmacytoid dendritic cells via toll-like receptor 7/9 and STING is mediated by melanoma-derived immunosuppressive cytokines and metabolic driftFrontiers in immunologyJanuary 1, 2023Monti M, Ferrari G, Grosso V, Missale F, Bugatti M, Cancila V, Zini S, Segala A, La Via L, Consoli F, et al.expression of the E2.2 antigen. IHC was developed using SignalStainBoost IHC Detection rabbit (cod. #18653, Cell Signaling Technology) alkaline phosphatase-conjugated produced in horse and Vulcan Fast Red as

(10) Mapping the Tumor Microenvironment in TNBC and Deep Exploration for M1 Macrophages-Associated Prognostic GenesFrontiers in immunologyJanuary 1, 2022Xu B, Sun H, Song X, Liu Q, Jin W.incubated with AP-conjugated horse-anti-rabbit secondary antibody (Cell Signaling Technology; catalog no. 18653) for 30 min, followed by a 20-min incubation period with alkaline phosphatase substrate (Vector; catalog

(11) Prognostic biomarker SMARCC1 and its association with immune infiltrates in hepatocellular carcinomaCancer cell internationalDecember 22, 2021Cai X, Zhou J, Deng J, Chen Z.(dilution 1:300, GTX114777, GeneTex, Texas, USA) and a secondary antibody of anti-rabbit (dilution 1:1000, #18653, Cell Signaling Technologies, Massachusetts, USA). Then the tissue microarray was digitized using Aperio

(12) Accumulation of CD45RO+CD8+ T cells is a diagnostic and prognostic biomarker for clear cell renal cell carcinomaAgingMay 19, 2021Wu K, Zheng X, Yao Z, Zheng Z, Huang W, Mu X, Sun F, Liu Z, Zheng J.were preserved at 37°C for 0.5 hour and rinsed with PBS. After applying with the secondary antibody (18653, Cell Signaling Technologies, USA) labelled horseradish peroxidase, the slices were preserved at 37°C

(13) Downregulation of lncRNA H19 alleviates atherosclerosis through inducing the apoptosis of vascular smooth muscle cellsMolecular Medicine ReportsJuly 31, 2020Hui Sun, Qianqian Jiang, Li Sheng, Kai Cuisections were incubated with a horseradish peroxidase-conjugated secondary antibody (1:500; cat. no. 18653; Cell Signaling Technology, Inc.) at room temperature for 1 h, followed by incubation with the chromogen

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SignalStain ® Boost IHC Detection Reagent (AP, Rabbit) from Cell Signaling Technology

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Cell Signaling Technology

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SignalStain ® Boost IHC Detection Reagent (AP, Rabbit) from Cell Signaling Technology

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