RBC Lysis Buffer (10X) from BioLegend

Product Specs
ItemRBC Lysis Buffer (10X)
CompanyBioLegend
Price $206.00Supplier Page View Company Product Page
Catalog Number420302
Quantity500 mL
FormatThis red blood cell (RBC) lysis buffer is supplied as a 10X solution and should be diluted to 1X in deionized water. 100 ml of 10X concentrate will yield a quantity of 1X solution that is sufficient to lyse 500 samples

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Contrasting functions of CD73 and adenosine in CD8+ T-cell exhaustion during antitumor immunityOncoimmunologyDecember 31, 2026Juan Saavedra-Almarza, Solange Gouët, Felipe Malgue, Catalina Bascuñan, Andrés Hernández-Oliveras, Joaquín De la Rosa, Javiera Reyes-Alvarez, Camilo Villaman, José Corrales-Bermúdez, Justine Castañeda, et al.and perfused with RPMI+10% FBS, followed by centrifugation and resuspension in RBC lysis buffer (cat: 420302, BioLegend). After washing, the cells were resuspended in RPMI+10% FBS. Draining and non-draining inguinal

(2) Intratumoral Virus-Like Particles Containing a TLR9 Agonist Combined with Systemic αPD-1 Activate Tumor-Specific CD8+ T CellsCancer Res CommunMay 1, 2026Travis D. Fischer, Caitlin D. Lemke-Miltner, George J. Weiner70-μm strainer (Fisherbrand), and red blood cells were removed via RBC Lysis Buffer (BioLegend, cat. #420302). Unfractionated OT-1 splenocytes (2 × 105 per well) were plated in 96-well U-bottom plates and were

(3) BMAL1 modulates glutamine supply to control haematopoietic stem and progenitor cell expansionDevelopmentApril 15, 2026Tim Petzold, Lydia K. Lutes, Keila Navarro I. Batista, Antonia Konle, Bastien Baechler, Stéphane Jemelin, Holger Gerhardt, Rachel Golub, Christoph Scheiermann, Julien Y. BertrandBSA in PBS by pipetting, and then underwent red blood cell lysis using RBC Lysis Buffer (Biolegend, 420302) for 5 min at room temperature. Cells were then washed and blocked with FcR Blocking Reagent (mouse;

(4) Transcriptional and spatial profiling of fibroblasts from human lungs highlights CTHRC1+ cells as fibrogenic signaling hubs in fibrosisbioRxivApril 10, 2026N.D. Vanegas-Avendano, H. Chen, J. Wellmerling, J. Rodriguez-Lopez, A. Ghobashi, V. Peters, C. Sen, B. F. Reader, K. Shilo, B. Gomperts, et al.temperature with 1X RBC lysis buffer (distilled water, Gibco, 15230162; 10X RBC Lysis Buffer, BioLegend, 420302). The whole lung cell suspension (WLCS) was resuspended in 1X PBS for cell counting and viability assessment

(5) A fully human pan VL9 HLA-E TCRm antibody enables functional dissection of HLA-E biology and checkpoint signalingiScienceApril 9, 2026Soroush Ghaffari, Katherine Upchurch-Ange, Gizem Oter, Trivendra Tripathi, Susanne Gimlin, Debra Wawro Weidanz, Jim Middelburg, Thorbald van Hall, Jon A. Weidanz(IMDM),Cytiva,Cat#SH30228 Lympho prep,Stemcell,Cat#18061 Lysis buffer (red blood cells),Biolegend,Cat#420302 Matrigel,Millipore-Sigma,Cat#CLS354248 McCoy,ATCC,Cat#30-2007 MHC Monomers/Tetramers,In house,N/A MyeloCult™ H5100

(6) Pharmacologic DPP-4 inhibition promotes CD8⁺ T cell metabolic fitness to enhance anti-tumor activitybioRxivApril 3, 2026Oriana Y. Teran Pumar, Elton L VanNoy, Abigail Haffey, Durga Prasad Gannamedi, Christine Isabelle Rafie, Dylan Scott Lykke Lykke Harwood, Julia R. Benedetti, Christine Ann Pittman Pittman Ballard, Erika Ciervo, Pedro Henrique Assenza Tavares Assenza Tavares Coroa, et al.span at 340 g for 5 minutes. Pellets were treated with Red Blood Cell (RBC) lysis buffer (Biolegend #420302) for 5 minutes at room temperature, span again, and then resuspended in 500 μL PBS and aliquoted for

(7) Intraperitoneal CF33-hNIS combined with PD-L1 blockade eradicates gastric cancer peritoneal metastases and prevents recurrence via durable T cell memoryJ Immunother CancerApril 1, 2026Annie Yang, Zhifang Zhang, Anthony K Park, Shyambabu Chaurasiya, Sang-In Kim, Jianming Lu, Yoya Vashi, Jennifer Cillis, Hannah Valencia, Courtney Chen, et al.350xg for 10 min at 4°C and red blood cells (RBC) were lysed using RBC Lysis Buffer (Biolegend, catalog#420302). After washing twice, splenocytes were either analyzed using flow cytometry for memory T cells or co-cultured

(8) ZFP148 is a transcriptional repressor of cytolytic effector CD8+ T cell differentiationNature ImmunologyMarch 27, 2026Tong Xiao, Xingyu Chen, No-Joon Song, Ryan J. Brown, Anjun Ma, Jay K. Mandula, Amir Yousif, Yi Wang, Minh Quynh May Le, Jianying Li, et al.mechanically, washed once with ice-cold PBS, subjected to red blood cell lysis (BioLegend, cat. no. 420302), passed through 70-μm cell strainers, and resuspended as single-cell suspensions. For liver lymphocyte

(9) Delivering cytokine mRNA to secondary lymphoid organs for robust cancer immunotherapyResearch SquareMarch 25, 2026Willem Anbergen, Yuri van Elsas, Stijn R.J. Hofstraat, Merel M.A. Hofstraat, Jeroen Hendrikx, Jeroen Versteeg, Gijs W.B. Versteeg, Myrthe H A P Kapteijns, Branca M. Kapteijns, Branca Bartelet, et al.filtered (70 μm), washed, and centrifuged. Red blood cells were lysed in 1× RBC lysis buffer (BioLegend 420302) for 7 min on ice, quenched with flow buffer, and centrifuged at 400 × g for 3 min at 4 °C. Preparing

(10) FAK inhibition in ovarian cancer releases omega-3 fatty acids to program CXCL13-producing anti-tumor resident peritoneal macrophagesCell ReportsMarch 24, 2026Xiao Lei Chen, Kevin M. Tharp, Marjaana Ojalill, Duygu Ozmadenci, Antonia Boyer, Terrance J. Haanen, Christine Lawson, Hyojae J. Lee, Marvin Xia, Elise Tahon, et al.at 300 × g for 10 min at 4°C. Cell pellets were treated with red blood cell lysis buffer (BioLegend, 420302) for 5 min at room temperature, followed by the addition of PBS to terminate the reaction. Intact cells

(11) CX3CL1-CX3CR1 signaling orchestrates malignant progression of prostate cancer through luminal progenitor-macrophage crosstalkCell Oncol (Dordr)March 23, 2026Yu Jiang, Yuchen Guo, Lizhuang Han, Shiwei Wang, Fang Wang, Yongliang Zhao, Jiajia Wang, Junyan Han, Miaomiao Liu, Zhihua Liu, et al.BS-70-CS, Biosharp, Anhui, China) and subjected to red blood cell lysis using RBC Lysis Buffer (Cat# 420302, Biolegend, California, USA). After washing, cells were resuspended in FACS buffer (PBS containing 2%

(12) In vivo site-specific engineering to reprogram T cellsNatureMarch 18, 2026William A. Nyberg, Pierre-Louis Bernard, Wayne Ngo, Charlotte H. Wang, Jonathan Ark, Allison Rothrock, Gina M. Borgo, Gabriella R. Kimmerly, Jae Hyung Jung, Vincent Allain, et al.samples were collected in EDTA-coated tubes. Red blood cells were lysed with RBC lysis buffer (BioLegend, 420302) for 15 min at room temperature. B cell depletion models For experiments with MHC-intact mice, 8-week-old

(13) Comprehensive single-cell metabolic profiling identifies targets to sensitize triple-negative breast cancer to chemo-immunotherapyCell Rep MedMarch 17, 2026Ying Xu, Xin-Yi Liu, Hang Zhang, Li Chen, Han Wang, Zhi-Ming Shao, Yi Xiao, Yi-Zhou JiangCell Activation Cocktail (with Brefeldin A),BioLegend,Cat# 423304 RBC Lysis Buffer (10x),BioLegend,Cat# 420302 Intracellular Staining Permeabilization Wash Buffer (10x),BioLegend,Cat# 421002 Fixation Buffer,BioLegend,Cat#

(14) Inhibition of MAPK p38α overcomes the cancer immunosurveillance defect caused by FPR1 loss-of-function mutationCell Rep MedMarch 17, 2026Yuhong Pan, Liwei Zhao, Jiani Liu, Misha Mao, Ai-Ling Tian, Julie Le Naour, Sarah Adriana Scuderi, Hui Pan, Flora Doffe, Donal Naylor, et al.high-binding assay plates,Corning,Cat# 9018 "Red Blood Cell (RBC) Lysis Buffer, 10×",Biolegend,Cat# 420302 CTL-Test™ medium,Immunospot,Cat# CTLT-010 GlutaMAX™ Supplement,Life Technologies,Cat# 35050061 RIPA buffer

(15) Respiratory viral infections prime accelerated lung cancer growthCellMarch 11, 2026Wei Qian, Xiaoqin Wei, Andrew J. Barros, Xiangyu Ye, Haibo Zhang, Qing Yu, Samuel P. Young, Eric V. Yeatts, Yury Park, Chaofan Li, et al.15140122 Zombie NIR™ Fixable Viability Kit,BioLegend,Cat# 423106 RBC Lysis Buffer (10X),BioLegend,Cat# 420302 "Brefeldin A Solution (1,000X)",BioLegend,Cat# 420601 GP33 (33-41) peptide,AnaSpec,Cat# AS-61669 mRNA-Spike,UVA

(16) Antibiotic use in early life impairs MAIT cell–mediated immunity in adulthoodJournal of Experimental MedicineMarch 2, 2026Gabrielle R. LeBlanc, Adam L. Sobel, Jonathan Melamed, Dominic Haas, Eduard Ansaldo, Aiko M. Cirone, Elizabeth Murguia, Michael G. Constantinides5 mL of 37% Percoll (Sigma-Aldrich), centrifuged, resuspended in 2 mL RBC Lysis Buffer (BioLegend, 420302), incubated for 3 minutes at room temperature, diluted with 3 mL 3% DNase, centrifuged, and resuspended

(17) Environmentally driven immune imprinting protects against allergyNatureFebruary 26, 2026S. Erickson, B. Lauring, J. Cullen, R. Medzhitov40-µm filters, spun at 800g for 5 min at 4 °C and resuspended in red blood cell lysis buffer (Biolegend 420302) according to the manufacturer’s recommended protocol. Following red blood cell lysis, 45 ml of ice-cold

(18) Aggressive Cholesterol Lowering Normalizes Atherosclerosis Regression in Jak2V617F MiceJournal of Lipid ResearchFebruary 18, 2026Brian D. Hardaway, Trevor P. Fidler, Mojdeh Tavallaie, Cheng-Chieh Hsu, Sandra Schiavone, Tong Xiao, Ranran Wang, Nan Wang, Alan R. TallFlow cytometric analysis for blood leukocytes was performed by lysing in RBC lysis buffer (Biolegend 420302) for 2 min at room temperature before centrifuging at 3,000 g for 2 min at 4°C. Cells were washed in

(19) Tissue-resident macrophage survival depends on mitochondrial function regulated by SerpinB2 in chronic inflammationNat CommunFebruary 12, 2026Sathish Babu Vasamsetti, Samreen Sadaf, Mohammad A. Uddin, Jixing Shen, Ebin Johny, Awishi Mondal, Jonathan Florentin, Liqun Lei, Aleef Mannan, Krithika Sudhakar Rao, et al.rpm for 30 minutes. After red blood cell lysis for three minutes using RBC lysis buffer (BioLegend, #420302), blood cells were washed and resuspended in cold FACS buffer (PBS containing 0.5% BSA). Cell staining

(20) Development of a dendritic cell-targeted vaccine strategy using proximity-induced conjugationTheranosticsFebruary 11, 2026Zhidong Wang, Xiaolin Yang, Jianjiang Li, Guang Chen, Haodi Ma, Zhengshuang Xu, Yu J. CaoSplenocytes were isolated after homogenization and red blood cells lysed (RBC lysis buffer, Biolegend, 420302). The release of IFN-γ under antigen stimulation was detected using mouse IFN-γ ELISpot precoated Kit

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RBC Lysis Buffer (10X) from BioLegend

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RBC Lysis Buffer (10X) from BioLegend

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