TransfeX™ Transfection Reagent from ATCC

Product Specs
ItemTransfeX™ Transfection Reagent
CompanyATCC
Price $230.00Supplier Page View Company Product Page
Catalog NumberACS-4005
ApplicationsTransient transfection, stable transfection, gene expression, protein production, and genetic manipulation
TypeMedia Sera Reagents
Volume500 µL

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ATCC

10801 University Boulevard

Manassas, Virginia 20110-2209

United States

Phone: (703) 365-2700
Fax: (703) 365-2701

Company Profile

Website: http://www.atcc.org

(1) Aldh3a1-mediated detoxification of reactive aldehydes contributes to distinct muscle responses to amyotrophic lateral sclerosis progressionFree Radical Biology and MedicineJune 1, 2026Ang Li, Li Dong, Xuejun Li, Jianxun Yi, Jo-Yu Wu, Jianjie Ma, Jingsong Zhoumedium overnight before transfection of 3 μg GFP-MG53 plasmids with TransfeX transfection reagent (ATCC ACS-4005). We selected soleus derived myoblasts because they form multi-nuclei, lengthy myotubes more robustly

(2) HELZ2 Regulates ApoB mRNA Stability to Modulate Fatty Liver Disease and AtherosclerosisCirculationFebruary 10, 2026Yiao Jiang, Zhao ZhangTransfection of plasmids was carried out using Lipofectamine 2000 (Life Technologies) or TransfeX (ATCC, ACS-4005) according to the manufacturer’s instructions. For immunofluorescence assays, 0.5μg plasmid DNA was

(3) HELZ2 regulates APOB mRNA stability to modulate fatty liver disease and atherosclerosisCirculationDecember 25, 2025Yiao Jiang, Zhao ZhangTransfection of plasmids was carried out using Lipofectamine 2000 (Life Technologies) or TransfeX (ATCC, ACS-4005) according to the manufacturer’s instructions. For immunofluorescence assays, 0.5μg plasmid DNA was

(4) Insulin-Regulated Actin Dynamics is Disrupted in a Human Keratinocyte Model of Hailey Hailey DiseasebioRxivDecember 17, 2025Ruby Gupta, Akash Chinchole, Ngozi P. Paul, Mrinal K. Sarkar, Johann E. Gudjonsson, Ajay Verma, Rajini Raothen transfected into an immortalized KC line (N/TERT-2G) using the TransfeX transfection kit (ATCC # ACS4005) in the presence of JAK1/JAK2 inhibitor, baricitinib (10 μg/ml, AchemBlock # G-5743). GFP-positive single

(5) High-throughput in silico screen uncovers key regulators of 3D genome architecturebioRxiv : the preprint server for biologyDecember 11, 2025Bai J, Lyu Q, Tan J, Issner R, Tischer B, Liu H, Goel V, Ling X, Bernstein BE, Tsirigos A, et al.protocols52. Briefly, the sgRNAs were transfected into cells using TransfeX Transfection Reagent (ATCC, ACS-4005) according to the manufacturer’s protocol. In the case of CRISPR knockin cell lines, donor DNA and pX459-sgRNA

(6) The First CRISPR-Based Therapeutic (SL_1.52) for African Swine Fever Is Effective in SwineVirusesNovember 14, 2025Naveen Verma, Alison O’Mahony, Roky Mohammad, Dylan Keiser, Craig W. Mosman, Deric Holden, Kristin Starr, Jared Bauer, Bradley Bauer, Roypim Suntisukwattana, et al.target gene plasmid totaling 250 ng of DNA, along with 0.5 µL TransfeX Transfection Reagent (ATCC, cat# ACS-4005, Manassas, VA, USA) reagent in 50 µL Opti-MEM (Thermo Fisher Scientific, cat# 51985091, Waltham, MA,

(7) A composite motif in calcimembrin/C16orf74 dictates multimeric dephosphorylation by calcineurinNature CommunicationsNovember 12, 2025Devin A. Bradburn, Joana C. Reis, R.Yvette Moreno, Shariq Qayyum, Thibault Viennet, Haribabu Arthanari, Martha S. Cyert(Thermo Fisher Scientific 11668019). MCF7 cells were transfected using Transfex ATCC transfection reagent (ATCC ACS−4005). Plasmids DNA encoding CLMB subcloned into pcDNA3 or pcDNA5/FRT/TO between HindIII and BamHI cut

(8) IgG autoantibodies in bullous pemphigoid induce a pathogenic MyD88-dependent pro-inflammatory response in keratinocytesNat CommunAugust 6, 2025Lei Bao, Christian F. Guerrero-Juarez, Jing Li, Manuela Pigors, Shirin Emtenani, Yingzi Liu, Adrian P. Mansini, Yulu F. Wang, Aadil Ahmed, Norito Ishii, et al.immortalized KC line (N/TERT-2G) using the TransfeX transfection kit (ATCC, Manassas, Virginia, USA; Cat# ACS4005) in the presence of JAK1/JAK2 inhibitor, baricitinib (10 mg/ml, AchemBlock, Hayward, California, USA;

(9) A simplified two-plasmid system for orthogonal control of mammalian gene expression using light-activated CRISPR effectorBMC BiotechnolJuly 1, 2025Shruthi S. Garimella, Shiaki A. Minami, Anusha N. Khanchandani, Justin C. Abad Santos, Susannah R. Schaffer, Priya S. Shah300 ng DNA with the same ratios used with HEK293T cells. C2C12 cell transfections used TransfeX (ATCC ACS-4005) with a TransfeX: DNA volume to mass ratio of 2:1, according to manufacturer instructions. The plasmid

(10) TFE3 fusion oncoprotein condensates drive transcriptional reprogramming and cancer progression in translocation renal cell carcinomaCell ReportsApril 22, 2025Choon Leng So, Ye Jin Lee, Bujamin H. Vokshi, Wanlu Chen, Binglin Huang, Emily De Sousa, Yangzhenyu Gao, Marie Elena Portuallo, Sumaiya Begum, Kasturee Jagirdar, et al.(Addgene #112473) were gifts from Dr. Kevin White. UOK109 cells were transfected with ATCC TransfeX (ACS-4005, ATCC) following the manufacturer’s protocol. The cells were selected with 800 μg/mL G418 sulfate (30-234-CR,

(11) A nucleosome switch primes hepatitis B virus infectionCellApril 17, 2025Nicholas A. Prescott, Tracy Biaco, Andrés Mansisidor, Yaron Bram, Justin Rendleman, Sarah C. Faulkner, Abigail A. Lemmon, Christine Lim, Rachel Tiersky, Eralda Salataj, et al.R0182S T4 Ligase,NEB,Cat# M0202M T5 Exonuclease,NEB,Cat# M0663S TransfeX Transfection Reagent,ATCC,Cat# ACS-4005 TURBO DNase,Invitrogen,Cat# AM2238 α-amanitin,Tocris,Cat# 4025 Critical commercial assays,Critical

(12) Two-photon activation, deactivation, and coherent control of melanopsin in live cellsbioRxivMarch 31, 2025Carlos A. Renteria, Stephen A Boppart, Brian Tibble, Rishyashring R. Tibble, Rishyashring R Iyer, Jindou A Algrain, Eric J. Algrain, Eric J Aksamitiene, Yuan-Zhi Aksamitiene, Yuan-Zhi Robinson, et al.melanopsin-GFP cloned in the mammalian expression plasmid17,18. Thereafter, 8 μL of TransfeX (ATCC, ACS-4005) was added to the cocktail, and the mixture was left to sit for ~15 minutes. Following this period,

(13) ALDH3A1-mediated detoxification of reactive aldehydes contributes to distinct muscle responses to denervation and Amyotrophic Lateral Sclerosis progressionbioRxivDecember 7, 2024Ang Li, Li Dong, Xuejun Li, Jianxun Yi, Jianjie Ma, Jingsong Zhoumedium overnight before transfection of 3 μg GFP-MG53 plasmids with TransfeX transfection reagent (ATCC ACS-4005). We selected soleus derived myoblasts because they form multi-nuclei, lengthy myotubes more robustly

(14) The L27 domain of MPP7 enhances TAZ-YY1 cooperation to renew muscle stem cellsEMBO reportsDecember 1, 2024Shao A, Kissil JL, Fan CM.Biomedicals,Catalog #MP92850145 FGF2,R&D Systems,Catalog #3718-FB-010 TransfeX Reagent,ATCC,Catalog #ACS-4005 Lipofectamine 3000,Thermo Fisher Scientific,Catalog #L3000008 Y-27632,Tocris Bioscience,Catalog #1254 Blebbinstatin,Tocris Bioscience

(15) RELT Is Upregulated in Breast Cancer and Induces Death in Breast Cancer CellsBiomedicinesNovember 22, 2024Batiste M, Joy B, Yee CK, Cho L, Christensen A, Abed I, Nguyen K, Yanumula A, Chang H, Cho ED, et al.according to the manufacturer’s instructions. Similarly, TransfeX transfection reagent (ATCC, cat: ACS-4005) was used to transfect 231 cells according to the manufacturer’s recommendations. RNA extraction, cDNA synthesis

(16) A Leaky Deep Intronic Splice Variant in CLRN1 Is Associated with Non-Syndromic Retinitis PigmentosaGenes (Basel)October 24, 2024Maria Abu Elasal, Samer Khateb, Daan M. Panneman, Susanne Roosing, Frans P. M. Cremers, Eyal Banin, Dror Sharon, Asodu Sandeep Sarmawith empty pET01 plasmid, wild-type and mutant CLRN1 plasmids using (TransfeX™ Transfection Reagent, ACS-4005™, ATCC, Manassas, VA, USA) transfection reagent. Cells were harvested 48 h post-transfection, and the

(17) A critical role for IFN-β signaling for IFN-κ induction in keratinocytesFrontiers in LupusMay 6, 2024Bin Xu, Jon Musai, Yee Sun Tan, Grace A. Hile, William R. Swindell, Benjamin Klein, J. Tingting Qin, Mrinal K. Sarkar, Johann E. Gudjonsson, J. Michelle Kahlenbergtransfected into an immortalized keratinocyte line (N/TERTs) using the TransfeX transfection kit (ATCC, Cat# ACS4005). Single cells positive for green fluorescent protein (GFP) were sorted into 96-well plates using a

(18) HERC6 regulates STING activity in a sex-biased manner through modulation of LATS2/VGLL3 Hippo signalingiScienceFebruary 16, 2024Ranjitha Uppala, Mrinal K. Sarkar, Kelly Z. Young, Feiyang Ma, Pritika Vemulapalli, Rachael Wasikowski, Olesya Plazyo, William R. Swindell, Emanual Maverakis, Mehrnaz Gharaee-Kermani, et al.siRNA,Dharmacon,D-001910-01-05 siRNA Buffer,Dharmacon,B-002000-UB-100 siRNA Delivery media,Dharmacon,B-005000 TransfeX,ATCC,ACS4005 Pierce RIPA buffer,ThermoFisher Scientific,89900 Protease and phosphatase inhibitor cocktail,Sigma,36978 Taqman

(19) Transposable elements as tissue-specific enhancers in cancers of endodermal lineageNature CommunicationsSeptember 1, 2023Konsta Karttunen, Divyesh Patel, Jihan Xia, Liangru Fei, Kimmo Palin, Lauri Aaltonen, Biswajyoti Sahutransfection reagent (Transfectin (Bio-Rad, 170-3351) was used for HepG2 at a 1:3 ratio and TransfeX™ (ATCC, ACS-4005) was used for RPE at a 1:2 ratio) in Opti-MEM medium (Gibco, 11524456), incubated for 15 minutes at

(20) Single cell and spatial sequencing define processes by which keratinocytes and fibroblasts amplify inflammatory responses in psoriasisNature communicationsJune 12, 2023Ma F, Plazyo O, Billi AC, Tsoi LC, Xing X, Wasikowski R, Gharaee-Kermani M, Hile G, Jiang Y, Harms PW, et al.plasmid with proper insertion was transfected into N/TERTs using the TransfeX transfection kit (ATCC # ACS4005). Single cells positive for GFP were sorted into 96-well plates using a MoFlo Astrios #1 cell sorter

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TransfeX™ Transfection Reagent from ATCC

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TransfeX™ Transfection Reagent from ATCC

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