Arraystar SYBR Green qPCR Master Mix (ROX+), 5mL from Arraystar, Inc

Product Specs
ItemArraystar SYBR Green qPCR Master Mix (ROX+), 5mL
CompanyArraystar, Inc
Price $362.00Supplier Page View Company Product Page
Catalog NumberAS-MR-006-5
Quantity5mL
TypeMaster Mix
Research AreaPCR
ApplicationsqPCR

Specifications/Features

All components included, just mix with cDNA template; Convenient 2X concentrate; Hot start polymerase for high specificity; Stabilized for consistent high performance; ROX passive reference included.

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Arraystar, Inc

9430 Key West Avenue

Suite 128

Rockville, Maryland 20850

United States

Phone: 888-416-6343
240-314-0388
Fax: 240-238-9860

Company Profile

Website: http://www.arraystar.com

(1) Expression profile of circular RNAs in type 2 diabetes mellitus patients with retinopathyJournal of Diabetes InvestigationJanuary 28, 2026Wencheng An, Bingyan Wang, Fang Chen, Bo An, Shujing Zhou, Lin Jiang, Zhijun Cui, Xiaohong Ou, Wei Huang, Huixian Yanmicroarray analysis. qRT‐PCR Selected circRNAs were quantified by qRT‐PCR using a 2X PCR master mix (Arraystar: AS‐MR‐006‐5) on QuantStudio™ 5 Real‐time PCR System (Applied Biosystems), according to the manufacturer's instructions.

(2) Sequencing and bioinformatics analysis of exosome-derived miRNAs in mouse models of pancreatic injury induced by OSAFrontiers in PhysiologyNovember 11, 2025Jiuhuang Lan, Jiayi Lin, Yaopeng Guo, Meilin Ji, Yizhao Lin, Qingshi Chenmaterials were added into the PCR reaction tube and thoroughly mixed. 5µL of 2X PCR master mix (Arraystar: AS-MR-006–5), 0.5 µL of forward primer (10 µM), 0.5 µL of reverse primer (10 µM), and 8 µL of nuclease-free water

(3) The significance of m6A-circFOXK2 in CD4+T cells from patients with rheumatoid arthritis and circFOXK2 correlates with Th17 % and autophagy☆J Transl AutoimmunOctober 15, 2025Qing Luo, Zhiwei Wu, Qiuyun Xiao, Mengfan Lan, Shiqian Wang, Peng Fu, Biqi Fu, Zikun Huang, Junming LiqPCRs were run on ViiA 7 Real-time PCR System (Applied Biosystems) using 2 × PCR master mix (Arraystar:AS-MR-006-5). 2.5 Reverse-transcription quantitative PCR (RT-qPCR) After evaluating the CD4+T cells purity of

(4) Sperm tRNA-derived fragments expression is potentially linked to abstinence-related improvement of sperm qualityAsian Journal of AndrologySeptember 1, 2025Xi-Ren Ji, Rui-Jun Wang, Zeng-Hui Huang, Hui-Lan Wu, Xiu-Hai Huang, Hao Bo, Ge Lin, Wen-Bing Zhu, Chuan Huangwere selected for validation. For the remaining complementary DNA (cDNA), we used 2× PCR master mix (AS-MR-006-5; Arraystar) to quantify the selected tRFs following the manufacturer’s instructions.23 After adding

(5) miRNA-494 in Lymphocytes: A Promising Biomarker for Acute Ischemic StrokeRev NeurolAugust 29, 2025Zixian Xie, Ziping Han, Tong Shen, Liyuan Zhong, Junfen Fan, Rongliang Wang, Feng Yan, Haiping Zhao, Qingfeng Ma, Yumin LuoTranscriptase (P7040L; Enzymatics, Beijing, China). RT-qPCR was performed with a 2X PCR master mix (AS-MR-006-5, Arraystar, Rockville, MD, USA) on a QuantStudio5 Real-time PCR System (Applied Biosystems, Foster City,

(6) miRNA-494 in Lymphocytes: A Promising Biomarker for Acute Ischemic StrokeRevista de NeurologíaAugust 29, 2025Zixian Xie, Ziping Han, Tong Shen, Liyuan Zhong, Junfen Fan, Rongliang Wang, Feng Yan, Haiping Zhao, Qingfeng Ma, Yumin LuoTranscriptase (P7040L; Enzymatics, Beijing, China). RT-qPCR was performed with a 2X PCR master mix (AS-MR-006-5, Arraystar, Rockville, MD, USA) on a QuantStudio5 Real-time PCR System (Applied Biosystems, Foster City,

(7) Differential expression profiles and bioinformatics analysis of microRNAs in brown adipose tissue dysfunction induced by chronic intermittent hypoxia in obstructive sleep apneaFront Cell Dev BiolAugust 15, 2025Meilin Ji, Yaopeng Guo, Jinjie Zhang, Shu Lin, Liangyi Li, Qingshi Chenusing a QuantStudio5 Real-time PCR System. RT-qPCR was performed using 2×PCR master mix (Arraystar: AS-MR-006-5) on a QuantStudio5 System (Applied Biosystems). Reaction conditions: 95°C for 10 min; 40 cycles of 95°C

(8) M6A Modification and Transcription Analysis of LncRNA in Cerebral Ischemia/Reperfusion InjuryInternational Journal of GenomicsOctober 5, 2024Jierong Mo, Zhiquan Li, Zhengfei Yang, Zuhua Huang, Pengpeng Guo, Jianfeng Gao, Haiqiong xiao, Ping Ye, Haini Qin, Tianen Zhou, et al.Dynabeads M-280 Sheep Anti-Rabbit IgG (Invitrogen, 11203D), RNase inhibitor (Enzymatics, Y9240L) and 2X PCR master mix (Arraystar, AS-MR-006-5) according to the manufacturer's instructions. The primers (Table 1) of key LncRNA are as follows: 2.8.

(9) N6-methyladenosine methylation analysis of circRNAs in acquired middle ear cholesteatomaFrontiers in GeneticsJune 24, 2024Jun He, Ahmad Mahmoudi, Jacqueline Yao, Qiulin Yuan, Jinfeng Fu, Wei LiuArraystar SYBR® Green qPCR Master Mix (Arraystar, Rockville, MD, United States of America, Cat. No. ASMR-006-5) was used for quantification. Three replicates were tested for each sample. The data were normalized

(10) Unraveling the role of microRNAs: potential biomarkers for gestational diabetes mellitus revealed through RNA sequencing analysisArchives of Gynecology and ObstetricsMay 30, 2024Huizhen Lin, Xiao Chen, Lisui Wang, Tang Zhu, Xiaohui Feng, Xiaomei Liu, Haiying Chen, Si PanUSA), according to the manufacture’s protocol. qRT-PCR was performed with a 2× PCR master mix (Cat No. AS-MR-006-5, Arraystar, MD, USA) to quantify miRNA expression. After pre-denaturation at 95 ℃ for 10 min, 40 PCR

(11) Interleukin 21 Drives a Hypermetabolic State and CD4+ T-Cell–Associated Pathogenicity in Chronic Intestinal InflammationGastroenterologyMay 1, 2024Adebowale O. Bamidele, Shravan K. Mishra, Guilherme Piovezani Piovezani Ramos, Petra Hirsova, Emily E. Klatt, Leena M. Abdelrahman, Mary R. Sagstetter, Heidi M. Davidson, Patrick J. Fehrenbach, Lucía Valenzuela-Pérez, et al.Flux/Pak,Agilent Technologies,Cat#102416-100 Arraystar SYBR Green qPCR Mater Mix,ARRAYSTAR INC,Cat#AS-MR-006-5 NuRNA™ Human Central Metabolism PCR Array (Roche Light Cycler 480),ARRAYSTARINC,Cat#AS-NM-004-1-R Spike-in

(12) Comprehensive exosomal microRNA profile and construction of competing endogenous RNA network in autism spectrum disorder: A pilot studyBiomolecules and BiomedicineApril 1, 2024Sha Zhao, Yan Zhong, Fang Shen, Xinning Cheng, Xiaojuan Qing, Jiamin Liuperformed in a QuantStudio5 Real-time PCR System (Applied Biosystems) with 2× PCR master mix (Arraystar: AS-MR-006-5). Relative gene expression was analyzed using the 2−ΔΔCT method. Human U6 was used as endogenous control.

(13) Transfer RNA-derived small RNA tRF-Glu-CTC attenuates neointimal formation via inhibition of fibromodulinCellular & Molecular Biology LettersJanuary 3, 2024Qi-Lan Jiang, Jia-Ying Xu, Qing-Ping Yao, Rui Jiang, Qin Xu, Bo-Tao Zhang, Tao Li, Jun JiangUSA). The cDNA samples were hybridized with PCR-specific primers using the 2X PCR Master Mix (catalog# AS-MR-006-5. Arraystar) and amplification protocols were performed on the QuantStudio 5 Real-Time PCR System (Applied

(14) N 2-methylguanosine modifications on human tRNAs and snRNA U6 are important for cell proliferation, protein translation and pre-mRNA splicingNucleic Acids ResearchAugust 11, 2023Can Wang, Nathalie Ulryck, Lydia Herzel, Nicolas Pythoud, Nicole Kleiber, Vincent Guérineau, Vincent Jactel, Chloé Moritz, Markus T Bohnsack, Christine Carapito, et al.nrStar Human tRNA PCR Array (Arraystar, #AS-NR-001–1) and Arraystar SYBR Green qPCR Master Mix(ROX+) (Arraystar; #AS-MR-006–5). The data were analyzed by the ΔCt method. The ΔCt values were calculated for each tRNA using the following

(15) Analysis of mRNA m6A modification and mRNA expression profiles in middle ear cholesteatomaFront GenetAugust 7, 2023Shumin Xie, Li Jin, Jun He, Jinfeng Fu, Tuanfang Yin, Jihao Ren, Wei Liugenerate cDNA, and the Arraystar SYBR® Green qPCR Master Mix (Arraystar, Rockville, MD, USA, Cat. No. ASMR-006-5) was used for quantification. Three replicates were tested for each sample. Data were normalized to

(16) CircRNA expression profiles and functional analysis in a mouse model of chronic intermittent hypoxia-induced renal injury: new insight into pathogenesisPeerJFebruary 28, 2023Qingshi Chen, Zhenzhen Hong, Zhiyu Chen, Yanfeng Chen, Dexin Liu(18080-044; Invitrogen, Waltham, MA, USA). According to the manufacturer’s instructions, 2X PCR Master Mix (AS-MR-006-5; Arraystar, Rockville, MD, USA) and ViiA 7 Real-Time PCR system (Applied Biosystems, Waltham, MA, USA)

(17) Men who inject opioids exhibit altered tRNA-Gly-GCC isoforms in semenMolecular Human ReproductionFebruary 28, 2023Germán Gornalusse, Ryan M Spengler, Erin Sandford, Yeseul Kim, Claire Levy, Muneesh Tewari, Florian Hladik, Lucia Vojtechfrom each donor (in a 1:40 dilution) were mixed with Arraystar SYBR® Green qPCR Master Mix (ROX+) (AS-MR-006-5, Arraystar, Rockville, MD, USA) and loaded onto the 384-well PCR array. As controls, we included: genomic

(18) Differential expression profiling of tRNA-Derived small RNAs and their potential roles in methamphetamine self-administered ratsFrontiers in GeneticsFebruary 2, 2023Yun Zhou, Qingxiao Hong, Wenjin Xu, Weisheng Chen, Xiaohu Xie, Dingding Zhuang, Miaojun Lai, Dan Fu, Zemin Xu, Majie Wang, et al.RNA-seq were included. The amplified cDNA was mixed with Arraystar SYBR® Green qPCR Master Mix (ROX+) (AS-MR-006-5, Arraystar). Serially diluted DNA templates were prepared for standard curve drawing. All cDNA samples

(19) Differentially expressed tRNA-derived fragments and their roles in primary cardiomyocytes stimulated by high glucoseFront Endocrinol (Lausanne)January 13, 2023Yongting Zhao, Ruxin Wang, Qi Qin, Jiaojiao Yu, Hui Che, Lihong Wanghybridization, 5’ adaptor ligation and reverse transcription. Arraystar SYBR® Green qPCR Master Mix (ROX+) (AS-MR-006-5, Arraystar, USA) was used to perform qRT-PCR, amplifying and quantifying the cDNA according to the manufacturer’s

(20) Circular RNAs expression profiles and bioinformatics analysis in bronchopulmonary dysplasiaJournal of Clinical Laboratory AnalysisJanuary 1, 2023Yu Lun, Junlong Hu, Yang Zuming20.0 μl with RNase‐free water. QRT‐PCR analyses were carried out using 2 × PCR Master Mix (Arraystar: AS‐MR‐006‐5). NCBI Primer‐BLAST and Generay Biotech were utilized to design and manufacture all primers. The 20

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Arraystar SYBR Green qPCR Master Mix (ROX+), 5mL from Arraystar, Inc

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Arraystar, Inc

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Arraystar SYBR Green qPCR Master Mix (ROX+), 5mL from Arraystar, Inc

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