Description
TMB ELISA Substrate is a ready-to-use chromogenic substrate for detection of horseradish peroxidase (HRP) activity. HRP can catalyze 3,3',5,5'-tetramethylbenzidine (TMB) producing a blue color with a maximal absorbance is at 370 nm or 620-652 nm. When stop solution is added, the solution will turn yellow and can be measured at 450 nm