T7 Express Competent E. coli (High Efficiency) from New England Biolabs

Product Specs
ItemT7 Express Competent E. coli (High Efficiency)
CompanyNew England Biolabs
Price $170.00 Inquire
Catalog NumberC2566I
Quantity6 x 0.2 ml
GenotypefhuA2 lacZ::T7 gene1 [lon] ompT gal sulA11 R(mcr-73::miniTn10--Tet^S)2 [dcm] R(zgb-210::Tn10--Tet^S) endA1 ?(mcrC-mrr)114::IS10
ApplicationsProteomics & Glycomics
SpeciesE. coli
Cell Type (standardized)Competent Cells
Validated Species ReactivityEscherichia
Temperature-80 C
Cell TypeCompetent Cells

Specifications/Features

Transformation Efficiency:
0.6-1 x 10⁹ cfu/µg

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Revisiting the evolution of the yeast Atg1 complexAutophagy ReportsDecember 31, 2025Kha M. Nguyen, Hannah R. Shariati, Calvin K. Yipexpression and protein purification Atg17 constructs were expressed in T7 Express Escherichia coli (NEB, C2566I), grown in 2x YT medium at 37°C to an OD600 of ~ 0.6, induced with 0.5 mM IPTG (Gold Biotechnology,

(2) Enzymatic carbon-fluorine bond cleavage by human gut microbesProc Natl Acad Sci U S AJune 17, 2025Silke I. Probst, Florian D. Felder, Victoria Poltorak, Ritesh Mewalal, Ian K. Blaby, Serina L. Robinsontransformed into E. coli T7 Express cells for protein production (New England Biolabs Frankfurt, Germany, NEB C2566I). The second batch of synthesized genes for colorimetric assay validation (n = 11) were cloned by the

(3) A multi-kingdom genetic barcoding system for precise clone isolationNature BiotechnologyMay 21, 2025Soh Ishiguro, Kana Ishida, Rina C. Sakata, Minori Ichiraku, Ren Takimoto, Rina Yogo, Yusuke Kijima, Hideto Mori, Mamoru Tanaka, Samuel King, et al.marker-based systems, plasmids were introduced into T7 Express chemically competent E. coli cells (NEB, no. C2566I), following the manufacturer’s high-efficiency transformation protocols. Transformants were selected

(4) Structural basis of BAK sequestration by MCL-1 in apoptosisMolecular CellApril 17, 2025Shagun Srivastava, Giridhar Sekar, Adedolapo Ojoawo, Anup Aggarwal, Elisabeth Ferreira, Emiko Uchikawa, Meek Yang, Christy R. Grace, Raja Dey, Yi-Lun Lin, et al.(DE3),Thermo Fisher Scientific,Cat# C601003 Escherichia coli strain: T7 Express,New England Biolabs,Cat# C2566I Escherichia coli strain : NEB 5-alpha,New England Biolabs,Cat# C2987H Escherichia coli strain : Stbl3,Invitrogen,Cat#

(5) Vibrio cholerae RbmB is an α-1,4-polysaccharide lyase with biofilm-disrupting activity against Vibrio polysaccharide (VPS)PLOS PathogensDecember 2, 2024Ranjuna Weerasekera, Alexis Moreau, Xin Huang, Kee-Myoung Nam, Alexander J. Hinbest, Yun Huynh, Xinyu Liu, Christopher Ashwood, Lauren E. Pepi, Eric Paulson, et al.chemical synthesis. Materials and methods Strains used in this study NEB5α E. coli (C2987I) and T7 Express E. coli (C2566I) were obtained from New England Biolabs, Ipswich, MA. The V. cholerae strain used in this study was

(6) The PWWP domain and the evolution of unique DNA methylation toolkits in HymenopteraiScienceNovember 17, 2023Robert Kucharski, Nancy Ellis, Tomasz P. Jurkowski, Paul J. Hurd, Ryszard Maleszkastrains,Bacterial and virus strains,Bacterial and virus strains ,, E.coli ER2566,New England Biolabs,Cat#C2566I ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(7) Structure-function analysis of ceTIR-1/hSARM1 explains the lack of Wallerian axonal degeneration in C. elegansCell ReportsSeptember 1, 2023Tami Khazma, Atira Grossman, Julia Guez-Haddad, Chengye Feng, Hadas Dabas, Radhika Sain, Michal Weitman, Ran Zalk, Michail N. Isupov, Marc Hammarlund, et al.and virus strains,Bacterial and virus strains,Bacterial and virus strains ,, T7 Express E. coli,NEB,C2566I ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(8) BAG3 regulates the specificity of the recognition of specific MAPT species by NBR1 and SQSTM1bioRxiv : the preprint server for biologyFebruary 9, 2023Lin H, Sandkuhler S, Dunlea C, King DH, Johnson GVW.minor adjustments [25]. His-MAPT and GST was prepared by transforming T7 Express Competent E. coli (#C2566I, NEB) with pT7-htau40, or pGEX6P inducing with IPTG, followed by resuspension in lysis buffer. To purify

(9) Ethanol tolerance of Clostridium thermocellum: the role of chaotropicity, temperature and pathway thermodynamics on growth and fermentative capacityMicrobial Cell FactoriesDecember 25, 2022Teun Kuil, Johannes Yayo, Johanna Pechan, Jan Küchler, Antonius J. A. van Marisin CTFUD medium [54]. Escherichia coli BL21 derivative cultures (New England Biolabs catalog number C2566I; purchased from BioNordika AB, Solna, Sweden) for plasmid cloning were propagated in LB medium (10 g

(10) Small molecule SJ572946 activates BAK to initiate apoptosisiScienceOctober 21, 2022Sekar G, Singh G, Qin X, Guibao CD, Schwam B, Inde Z, Grace CR, Zhang W, Slavish PJ, Lin W, et al.(DE3),Thermo Fisher Scientific,Cat# C601003 Escherichia coli strain: T7 Express,New England Biolabs,Cat# C2566I ,, Biological samples,Biological samples,Biological samples ,, None,N/A,N/A ,, "Chemicals, peptides

(11) Divergent binding mode for a protozoan BRC repeat to RAD51Biochemical JournalMay 27, 2022Teodors Pantelejevs, Marko HyvönenReagent,Source,Identifier T7 Express E. coli cells,New England Biolabs,C2566I BL21(DE3) E. coli cells,New England Biolabs,C2530H FAM-dT60 oligonucleotide,Sigma–Aldrich,n/a TEV

(12) Poxviruses and paramyxoviruses use a conserved mechanism of STAT1 antagonism to inhibit interferon signalingCell host & microbeMarch 9, 2022Talbot-Cooper C, Pantelejevs T, Shannon JP, Cherry CR, Au MT, Hyvönen M, Hickman HD, Smith GL.and virus strains,Bacterial and virus strains ,, T7 Express competent E. coli,New England Biolabs,C2566I E. coli (subcloning efficiency DH5α competent Cells),Invitrogen,18265-017 VACV strain Western Reserve:

(13) Functional Analysis of H ⁺ -Pumping Membrane-Bound Pyrophosphatase, ADP-Glucose Synthase, and Pyruvate Phosphate Dikinase as Pyrophosphate Sources in Clostridium thermocellumApplied and Environmental MicrobiologyFebruary 22, 2022Teun Kuil, Shuen Hon, Johannes Yayo, Charles Foster, Giulia Ravagnan, Costas D. Maranas, Lee R. Lynd, Daniel G. Olson, Antonius J. A. van Marisstrains used in this study (Table 4) originate from a BL21 derivative (New England Biolabs catalog number C2566I; purchased from BioNordika AB, Solna, Sweden). Stock cultures were propagated in LB medium (10 g L−1

(14) Single-stranded DNA binding proteins influence APOBEC3A substrate preferenceScientific ReportsDecember 1, 2021Amber L. Brown, Christopher D. Collins, Secily Thompson, Margo Coxon, Tony M. Mertz, Steven A. Robertshuman RPA was expressed from plasmid p11d-tRPA(123), (Addgene; 102613), in T7 express E. coli (NEB; C2566I). Human RPA was purified following protocols previously described in70, using chromatography on subsequent

(15) Neurotoxins subvert the allosteric activation mechanism of SARM1 to induce neuronal lossCell ReportsOctober 19, 2021Tong Wu et al.Strains Stellar™ Competent Cells,Takara Bio,Cat# 636763 T7 Express Competent E. coli,New England BioLabs,Cat# C2566I "Chemicals, Peptides, and Recombinant Proteins","Chemicals, Peptides, and Recombinant Proteins","Chemicals,

(16) Multiplexed functional metagenomic analysis of the infant microbiome identifies effectors of NF-κB, autophagy, and cellular redox stateCell ReportsSeptember 21, 2021Frank J. Piscotta, Shawn T. Whitfield, Toshiki G. Nakashige, Andreia B. Estrela, Thahmina Ali, Sean F. Bradystrains ,, Escherichia coli EC100,Lucigen,Cat#EC10010 T7 Express Escherichia coli,New England Biolabs,Cat#C2566I ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(17) Peptide-tiling screens of cancer drivers reveal oncogenic protein domains and associated peptide inhibitorsCell SystemsJuly 1, 2021Kyle M. Ford, Rebecca Panwala, Dai-Hua Chen, Andrew Portell, Nathan Palmer, Prashant Maliwere cloned into the pET Champion vector (Thermo K630203) and expressed in T7 express E. coli (NEB C2566I). Constructs were ordered as gBlocks from IDT and cloned directly into the vector via Gibson Assembly.

(18) Interactions of the C-Terminal Truncated DEAD-Box Protein DDX3X With RNA and Nucleotide SubstratesACS OmegaMay 18, 2021Anthony F. T. Moore, Aliana López de Victoria, Eda Koculial.13 In brief, the DDX3X construct bearing an N-terminal His-tag was expressed in Escherichia coli C2566I (NEB). Nickel affinity column (HisPur Ni-NTA Superflow Agarose, Thermo Scientific) and size-exclusion

(19) Laboratory Evolution and Reverse Engineering of Clostridium thermocellum for Growth on Glucose and FructoseApplied and Environmental MicrobiologyApril 13, 2021Johannes Yayo, Teun Kuil, Daniel G. Olson, Lee R. Lynd, Evert K. Holwerda, Antonius J. A. van Marisplasmid using Gibson assembly (51) in an E. coli BL21 derivate (New England Biolabs catalog number C2566I purchased from BioNordika AB, Solna, Sweden). Purification of PCR amplicons and plasmid DNA was done

(20) Single-strand DNA breaks cause replisome disassemblyMolecular CellMarch 1, 2021Kyle B. Vrtis, James M. Dewar, Gheorghe Chistol, R. Alex Wu, Thomas G. W. Graham, Johannes C. WalterCells,Expression Systems,Cat#94-001S Tni Insect Cells,Expression Systems,Cat#94-002S T7 Express Cells,NEB,Cat# C2566I Experimental Models: Organisms/Strains,Experimental Models: Organisms/Strains,Experimental Models:

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