T7 Express Competent E. coli (High Efficiency) from New England Biolabs

Product Specs
ItemT7 Express Competent E. coli (High Efficiency)
CompanyNew England Biolabs
Price $225.00 Inquire
Catalog NumberC2566H
Quantity20 x 0.05 ml
ApplicationsProteomics & Glycomics
Cell Type (standardized)Competent Cells
Validated Species ReactivityEscherichia
SpeciesE. coli
Temperature-80 C
Cell TypeCompetent Cells
GenotypefhuA2 lacZ::T7 gene1 [lon] ompT gal sulA11 R(mcr-73::miniTn10--TetS)2 [dcm] R(zgb-210::Tn10--TetS) endA1 ?(mcrC-mrr)114::IS10

Specifications/Features

Transformation Efficiency:
2 - 6 x 10⁸ cfu/µg

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Utilizing Engineered Monobodies for the Electrochemical Quantification of LysozymeAnal ChemDecember 23, 2025Sunanda Dey, Andrew Naser, Daniel R. Woldring, David P. HickeyThe pCT and Pet22b plasmids were obtained from IDTDNA and expressed in T7 (E. coli) cells (NEB Cat: C2566H). LB and kanamycin solution were used to cultivate starting cultures of T7 cells, which were grown overnight.

(2) Structural insights into outer membrane protein biogenesis in pathogenic NeisseriaStructureNovember 6, 2025Evan Billings, Zixing Fan, Moloud Aflaki Sooreshjani, James C. Gumbart, Nicholas Noinajexpression, 100 ng of pNgBamAE and pNgBamCD plasmid were co-transformed into T7 Express cells (NEB # C2566H) and plated on an LB-agar plate containing 50 μg/mL of streptomycin (strep) and 50 μg/mL of carbenicillin.

(3) Structural mechanism of RECQ1 helicase in unfolding G-quadruplexes compared with duplex DNANucleic Acids ResearchSeptember 12, 2025Ze-Yu Song, Xin Zhang, Xia Ai, Ling-Yun Huang, Xi-Miao Hou, Philippe Fossé, Na-Nv Liu, Olivier Mauffret, Stéphane Réty, Xu-Guang Xigenerating His-SUMO-BtRECQ1. The recombinant plasmid was transformed into the Escherichia coli strain C2566H (New England Biolabs). Bacterial cultures were grown in Luria broth containing 100 μg/ml ampicillin

(4) RABGAP1 is a sensor that facilitates the sorting and processing of amyloid precursor proteinThe EMBO JournalAugust 26, 2025Jessica Eden, Jonathan G G Kaufman, Conceição Pereira, Eleanor Fox, Jerome Cattin-Ortolá, Lorena Benedetti, Bart Nieuwenhuis, David J Owen, Jennifer Lippincott-Schwartz, Sean Munro, et al.fused to the cytosolic tail of APP (either WT or a mutant) was expressed in T7 express E. coli (NEB, C2566H) and grown in 1 L of shaking 2xTY at 37 °C, to an OD600 of 0.7. Expression was induced with 0.5 mM IPTG

(5) Verrucomicrobium spinosum essential genome and the divergence of cell division in the PVC superphylumiScienceAugust 15, 2025Valentina Henriques, David Moyano-Palazuelo, Maria Teresa Alonso-Pascual, Manuel Pazos, Damien P. Devos, Elena Rivas-MarinR(mcr-73::miniTn10--TetS)2 [dcm] R(zgb-210::Tn10--TetS) endA1 Δ(mcrC-mrr)114::IS10,New England Biolab,Catalog #: C2566H Verrucomicrobium spinosum,DSMZ,DSM 4136 V. spinosum DV086Genotype:VSP_RS42430::pDV134,This work,N/A V.

(6) A blueprint for biomolecular condensation driven by bacterial microcompartment encapsulation peptidesNat CommunAugust 11, 2025Daniel S. Trettel, Cesar A. López, Eliana Rodriguez, Babetta L. Marrone, Cesar Raul Gonzalez-Esquertransformation. Protein expression and purification Appropriate plasmids were transformed fresh into NEB T7 Express (#C2566H) competent E. coli. Single colonies were used to inoculate 4 mL of 2 × YT broth and carbenicillin antibiotic

(7) A high effective expression of human D-glucuronyl C5-epimerase with dimer structure in Escherichia coliFrontiers in MicrobiologyJuly 31, 2025Qin-xia Song, Li-jian GuoExpression of Glce truncated proteins in Escherichia coli The recombinant plasmids were transformed into C2566H E. coli competent cells (New England Biolabs) for expression of SUMO-Glce102-617 and SUMO-Glce167-617

(8) Functional characterization of DPP4 and FcRn as receptor and coreceptor for classical human astroviruses in Caco-2 cellsPLoS pathogensJuly 1, 2025Aguilera-Flores C, Valencia-Morales MDP, López T, Moreno-Contreras J, Lentz A, Espinoza MA, DuBois RM, López S, Arias CF.#71554). The plasmids were heat-shock transformed into T7 Express E. coli cells (New England Biolabs #C2566H). A culture was grown in LB media with ampicillin via shaking at 37oC. Upon reaching an OD600 of 0.6,

(9) A distinct class of ferredoxin:NADP+ oxidoreductase enzymes driving thermophilic ethanol productionJournal of Biological ChemistryJuly 1, 2025Shu Huang, Syed Muhammad Saad Imran, Anthony A. Lanahan, Sarah K. Hammer, Carolyn E. Lubner, Lee R. Lynd, Daniel G. Olsonwas used for the overexpression of Fnor enzymes for purification. E. coli T7 Express (NEB catalog #C2566H) was used for isolating plasmid DNA for C. thermocellum transformation. E. coli G8EB7 (MG1655 ΔiscR::kana

(10) Therapeutic potential of allosteric HECT E3 ligase inhibitionCellMay 15, 2025Alexander M.K. Rothman, Amir Florentin, Florence Zink, Catherine Quigley, Olivier Bonneau, Rene Hemmig, Amanda Hachey, Tomas Rejtar, Maulik Thaker, Rishi Jain, et al.virus strains,Bacterial and virus strains ,, T7 Express Competent E. coli,New England Biolabs,Cat#C2566H Mach1T1R E. coli,Thermo Fisher Scientific,Cat#C862003 ,, Biological samples,Biological samples,Biological

(11) IgAim: cell surface Ig-aimed immune memory erasers for the therapy of autoimmune diseases and B leukemiaNAR Molecular MedicineApril 30, 2025Hiroshi Arakawaexpression vectors were transformed into T7 Express Competent Escherichiacoli (High Efficiency) (NEB, C2566H). Logarithmically growing bacteria were incubated with 0.4 mM Isopropyl β-D-1-thiogalactopyranoside

(12) NAL1 forms a molecular cage to regulate FZP phase separationProc Natl Acad Sci U S AApril 15, 2025Ling-Yun Huang, Ting-Ting Wang, Peng-Tao Shi, Ze-Yu Song, Wei-Fei Chen, Na-Nv Liu, Xia Ai, Hai-Hong Li, Xi-Miao Hou, Li-Bing Wang, et al.and FZPs [The full-length FZP (NCBI Gene ID: 4344233) and its truncated proteins] were expressed in C2566H Escherichia coli cells (New England Biolabs) and then purified by the nickel-nitrilotriacetic acid column

(13) Structures of respiratory syncytial virus G bound to broadly reactive antibodies provide insights into vaccine designSci RepMarch 13, 2025Maria G. Juarez, Sara M. O'Rourke, John V. Dzimianski, Delia Gagnon, Gabriel Penunuri, Vitor H. B. Serrão, Russell B. Corbett-Detig, Lawrence M. Kauvar, Rebecca M. DuBoisMethod 1: Recombinant RSV G CCD was expressed in T7Express E. coli cells (New England Biolabs, REF C2566H) cells overnight at 18 °C. Cells were lysed by ultrasonication in wash buffer (20 mM Tris-Cl pH 8.0,

(14) Arginine methylation of the p30 C/EBPα oncoprotein regulates progenitor proliferation and myeloid differentiationiScienceNovember 15, 2024Nguyen LT, Zimmermann K, Kowenz-Leutz E, Dörr D, Schütz A, Schönheit J, Mildner A, Leutz A.strains,Bacterial and virus strains,Bacterial and virus strains ,, E. coli T7 Express cells,NEB,#C2566H Platinum-E Retroviral Packaging Cell Lines,N/A,N/A ,, "Chemicals, peptides, and recombinant proteins","Chemicals,

(15) A combinatorially complete epistatic fitness landscape in an enzyme active siteProceedings of the National Academy of SciencesAugust 6, 2024Kadina E. Johnston, Patrick J. Almhjell, Ella J. Watkins-Dulaney, Grace Liu, Nicholas J. Porter, Jason Yang, Frances H. ArnoldAssays. Protein was expressed for in vitro assays in T7 Express cells (New England Biolabs, Catalog # C2566H) in 96-well deep-well plates using the pET22b(+) vector. Colonies were picked into each well and grown

(16) Control of DNA replication in vitro using a reversible replication barrierNature ProtocolsJuly 1, 2024Emma J. Vontalge, Tamar Kavlashvili, Steven N. Dahmen, Matthew T. Cranford, James M. DewarFigs 6 and 7). MATERIALS Biological material:E. coli strains T7 Express E. coli cells (NEB, cat. no. C2566H)DH5α E. coli for subcloning (Thermo Scientific, cat. no. EC0111) Reagents General reagents: Bacto

(17) A communication hub for phosphoregulation of kinetochore-microtubule attachmentCurrent BiologyJune 3, 2024Jacob A. Zahm, Stephen C. Harrisonstrains,Bacterial and virus strains,Bacterial and virus strains T7-express E. Coli,New England Biolabs,C2566H DH5α E. Coli,New England Biolabs,C2987H "Chemicals, peptides, and recombinant proteins","Chemicals,

(18) Carotenoid cleavage enzymes evolved convergently to generate the visual chromophoreNature Chemical BiologyJune 1, 2024Yasmeen J. Solano, Michael P. Everett, Kelly S. Dang, Jude Abueg, Philip D. Kiserof some NinaB homologs was also tested in T7 express E. coli competent cells (New England Biolabs, C2566H) that were co-transformed with the chaperone overexpression plasmid pG-KJE8 (Takara Bio, 3340). NinaB

(19) Structural insights into the N-terminal APHB domain of HrpA: mediating canonical and i-motif recognitionNucleic Acids ResearchApril 12, 2024Ben-Ge Xin, Ling-Yun Huang, Ling-Gang Yuan, Na-Nv Liu, Hai-Hong Li, Xia Ai, Dong-Sheng Lei, Xi-Miao Hou, Stephane Rety, Xu-Guang Xiresulting in the creation of His-sumo-HrpA. The recombinant plasmid was subsequently transformed into the C2566H E. coli strain (New England Biolabs). Cultures were grown in Luria broth (LB) supplemented with 100

(20) A method for rapid nanobody screening with no bias of the library diversityiScienceFebruary 16, 2024Zhiqing Tao, Xiaoling Zhao, Huan Wang, Juan Zhang, Guosheng Jiang, Bin Yu, Yihao Chen, Mingjun Zhu, Junli Long, Lei Yin, et al.strains,Bacterial and virus strains,Bacterial and virus strains ,, T7 Express Competent E. coli,NEB,Cat#C2566H SHuffle® T7 Express Competent E. coli,NEB,Cat#C3029J ,, Biological samples,Biological samples,Biological

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