BL21 Competent E. coli from New England Biolabs

Product Specs
ItemBL21 Competent E. coli
CompanyNew England Biolabs
Price $225.00 Inquire
Catalog NumberC2530H
Quantity20 x 0.05 ml
GenotypefhuA2 [lon] ompT gal [dcm] delta hsdS
ApplicationsProteomics & Glycomics
Cell Type (standardized)Competent Cells
Validated Species ReactivityEscherichia
SpeciesE. coli
Temperature-80 C
Cell TypeCompetent Cells

Specifications/Features

Transformation Efficiency:
1–5 x 107 cfu/µg pUC19 DNA

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Biogenesis and downstream effects of 3',5' and 2',3' cAMP isomers in plantsSci AdvMay 8, 2026Mingyue Li, Monika Chodasiewicz, Malavika Muraleedharan, Israel M. Lopez, Michal Gorka, Olga Kerber, Saqer S. Alotaibi, Andrew D.L. Nelson, Rene Lenobel, Jaroslava Friedecká, et al.primers listed in table S1. The resulting plasmids were introduced into BL21 competent cells (NEB, C2530H) to produce MBP-fusion proteins with additional N-terminal 6 × His tags. The transformants were grown

(2) Host-transposon mutualism supports regeneration in planariansDevelopmental CellMarch 30, 2026Hae-Lim Lee, Axel Poulet, Sudheesh Allikka Allikka Parambil, Josien C. van Wolfswinkel[dcm] ΔhsdS λ DE3 = λ sBamHIo ΔEcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 Δnin5,New England Biolabs,Cat#C2530H "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(3) Disease-causing mutations in Synaptotagmin can act via dominant-negative, gain-of-function or haploinsufficient mechanismsbioRxivMarch 19, 2026Zhuo Guan, Maria Bykhovskaia, J. Troy Littletonand virus strains,Bacterial and virus strains E. coli: BL21 Competent cells,New England BioLabs,Cat# C2530H "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(4) LYPLAL1 Rare Loss-of-Function Variants in Humans and Deletion in Human Hepatoma Cells Protect Against MASLDJournal of Lipid ResearchMarch 3, 2026Rawdat Hussain, Chinmay Raut, Ponnandy Prabhu, Akram Irshaid, Yash Hegde, Yue Chen, Asmita Pant, Antonino Oliveri, Xiaochao Wei, Lishi Yin, et al.vector (Addgene, #27458001) and transformed into E. coli BL21 competent cells (New England Biolabs, #C2530H). Transformants were grown overnight in 5 ml LB medium containing 50 μg/ml ampicillin and 34 μg/ml chloramphenicol.

(5) Dock-and-lock binding of SxIP ligands is required for stable and selective EB1 interactionsElifeDecember 10, 2025Teresa Almeida, Eleanor Hargreaves, Tobias Zech, Igor Barsukovdomain of EB1, expression construct" "Strain, strain background (Escherichia coli)",BL21(DE3),NEB,C2530H,Chemically competent cells Cell line (human),HeLa,AATC,"PCS-201–012, RRID:CVCL_0030",Mycoplasma negative Transfected

(6) Dock-and-lock binding of SxIP ligands is required for stable and selective EB1 interactionsElifeDecember 10, 2025Teresa Almeida, Eleanor Hargreaves, Tobias Zech, Igor Barsukovdomain of EB1, expression construct" "Strain, strain background (Escherichia coli)",BL21(DE3),NEB,C2530H,Chemically competent cells Cell line (human),HeLa,AATC,"PCS-201–012, RRID:CVCL_0030",Mycoplasma negative Transfected

(7) Congenital heart disease missense mutations in the TBX5 DNA-binding domain alter thermal stability and DNA-binding affinityG3: Genes, Genomes, GeneticsOctober 8, 2025Alejandro Rivera-Madera, Edwin G Peña-Martínez, Jean L Messon-Bird, Diego A Pomales-Matos, Oswaldo L Echevarría-Bonilla, Leandro Sanabria-Alberto, Esther A Peterson-Peguero, José A Rodríguez-Martínezwhole-plasmid sequencing (Plasmidsaurus Inc.) and transformed into BL21 DE3 E. coli (New England Biolabs #C2530H or Millipore Sigma #70956-4). First, 50 mL Luria broth (Sigma-Aldrich, #L3022) cultures were grown for

(8) Teashirt and C-Terminal Binding Protein Interact to Regulate Drosophila Eye DevelopmentGenes (Basel)September 5, 2025Surya Jyoti Banerjee, Jennifer Curtiss, Chase Drucker, Harley Hinesinto the pGEX 4T-2 plasmids under the tac promoter. They were transformed into BL21 cells (Cat. No. C2530H, New England Biolabs, Ipswich, MA, USA). Each bait protein’s expression was induced in the BL21 cells

(9) Crystal structure of a seven-substitution mutant of hydroxynitrile lyase from rubber treeActa Crystallographica Section F Structural Biology CommunicationsSeptember 1, 2025Colin T. Pierce, Lauren R. Greenberg, Meghan E. Walsh, Ke Shi, Drenen J. Magee, Hideki Aihara, Wendy Gordon, Robert L. Evans, Romas J. Kazlauskasto confirm mutations. The sequence-confirmed plasmid was transformed into E. coli strain BL21 (NEB C2530H) chemically competent cells according to the NEB transformation protocol and plated onto LB plates containing

(10) β‐Catenin localization in the ctenophore Mnemiopsis leidyi suggests an ancestral role in cell adhesion and nuclear functionDevelopmental DynamicsSeptember 1, 2025Brian M. Walters, Lucas J. Guttieres, Mayline Goëb, Stanley J. Marjenberg, Mark Q. Martindale, Athula H. WikramanayakeAntigen preparation and antibody generation BL21(DE3) competent E. coli (New England Biolabs cat.# C2530H) were transformed with the Mlβ‐cat pGEX‐6P‐1 construct. Five milliliters of inoculated terrific broth

(11) Crystal structure of a seven-substitution mutant of hydroxynitrile lyase from rubber treeActa Crystallogr F Struct Biol CommunAugust 27, 2025Colin T. Pierce, Lauren R. Greenberg, Meghan E. Walsh, Ke Shi, Drenen J. Magee, Hideki Aihara, Wendy Gordon, Robert L. Evans, Romas J. Kazlauskasto confirm mutations. The sequence-confirmed plasmid was transformed into E. coli strain BL21 (NEB C2530H) chemically competent cells according to the NEB transformation protocol and plated onto LB plates containing

(12) Ubiquitination of the histone variant mH2A1.2 prevents toxic RAD18 accumulation at a subset of genomic loci upon replication stressMolecular CellAugust 21, 2025Maxime Galloy, Andréanne Blondeau, Élodie Vion, Daein Kim, Collin A. Bakker, Vincent Gaggioli, Mélissa Thomas, Élise G. Lavoie, Isabelle Marois, Alberto David Delgado Monterroso, et al.purified as previously described6. Briefly, plasmids were expressed in BL21 competent Escherichia coli (C2530H, New England Biolabs) and isolated by affinity purification using Ni-NTA Agarose (30230; QIAGEN). UBA1

(13) Phage-displayed synthetic library and screening platform for nanobody discoveryElifeAugust 1, 2025Baolong Xia, Ah-Ram Kim, Feimei Liu, Myeonghoon Han, Emily Stoneburner, Stephanie Makdissi, Francesca Di Cara, Stephanie E Mohr, Aaron Ring, Norbert Perrimon,One Shot ccdB Survival 2 T1R Competent Cells,Invitrogen,A10460, "Strain, strain background (Escherichia coli)",BL21 Competent E. coli,NEB,C2530H, Antibody,M13 Major Coat Protein Antibody (RL-ph1) HRP,Santa Cruz biotechnology,sc-53004 HRP,1:1000

(14) Signal peptide-independent secretion of keratin-19 by pancreatic cancer cellsProc Natl Acad Sci U S AJuly 8, 2025Philip Moresco, Jonathan P. Kastan, Jung-In Yang, Rishvanth Prabakar, Z. Larkin Kelley, Francesca Minicozzi, Dexter W. Adams, Paolo Cifani, David A. Tuveson, Douglas T. Fearonappropriate construct incorporation. Sequenced plasmids were next transfected into BL21 cells (NEB, C2530H) and transfected BL21s were expanded in LB with ampicillin at 37 °C with shaking until the OD600 reached

(15) Dynamic regulation of integrin β1 phosphorylation supports invasion of breast cancer cellsNature Cell BiologyMay 26, 2025James R. W. Conway, Omkar Joshi, Jasmin Kaivola, Gautier Follain, Michalis Gounis, David Kühl, Johanna IvaskaSoftware). Recombinant protein purification As described previously65, BL21 competent Escherichia coli (NEB, C2530H) were transformed with the pET28a-HaloTag-CNA35 plasmid and grown overnight on a shaker at 37 °C to

(16) Congenital heart disease missense mutations in the TBX5 DNA-binding domain alter thermal stability and DNA-binding affinityBIORXIVMay 17, 2025Alejandro Rivera-Madera, Edwin G. Peña-Martínez, Jean L. Messon-Bird, Diego A. Pomales-Matos, Oswaldo L. Echevarría-Bonilla, Leandro Sanabria-Alberto, Esther A. Peterson-Peguero, José A. Rodríguez-Martínezwhole-plasmid sequencing (Plasmidsaurus Inc.) and transformed into BL21 DE3 E. coli (New England Biolabs #C2530H or Millipore Sigma #70956–4). First, 50 mL Luria Broth (Sigma-Aldrich, #L3022) cultures were grown for

(17) Hyperactivation of RAB5 disrupts the endosomal Rab cascade leading to endolysosomal dysregulation in Down syndrome: A necessary role for increased APP gene doseAlzheimer's & dementia : the journal of the Alzheimer's AssociationMay 1, 2025Chen XQ, Zuo X, Becker A, Mobley WC.(ab65306), and Cathepsin D Activity Assay Kit (ab65302) were from Abcam. Escherichia coli strain BL21 (C2530H) was from New England Biolabs. RESEARCH IN CONTEXT Systematic review: It is well‐established that in

(18) Purification of Low-Complexity Domain Proteins FUS, EWSR1, and Their FusionsCurr ProtocApril 26, 2025Jesse J. Altemus, Michelle A. Lay, Valery F. Thompson, Jacob C. Schwartzelectrophoretic mobility shift assays (EMSA). Materials Chemically competent E. coli BL21 cells (NEB, cat. no. C2530H)Ice100 ng plasmid DNA appropriate for the protein expression intendedLB growth medium (Miller's) (RPI,

(19) Single-fluorogen imaging reveals distinct environmental and structural features of biomolecular condensatesNature PhysicsMarch 14, 2025Tingting Wu, Matthew R. King, Yuanxin Qiu, Mina Farag, Rohit V. Pappu, Matthew D. Lewprotein was stored at 4 °C until used. Expression and purification of DDX4-NT BL21 E. coli cells (NEB, C2530H) transfected with His-SUMO-DDX4-NT28, a gift from L. E. Kay, were grown in the same manner as A1-LCD.

(20) TIR1-produced cAMP as a second messenger in transcriptional auxin signallingNatureMarch 5, 2025Huihuang Chen, Linlin Qi, Minxia Zou, Mengting Lu, Mateusz Kwiatkowski, Yuanrong Pei, Krzysztof Jaworski, Jiří Frimlplasmids confirmed by sequencing were transformed into E. coli BL21 competent cells (NEB, catalogue no. C2530H) for protein purification. GST-ASK1 was obtained similarly. Expression and purification of the recombinant

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