BL21(DE3)pLysS Competent Cells, >10(6)cfu/ug from Promega

Product Specs
ItemBL21(DE3)pLysS Competent Cells, >10(6)cfu/ug
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberL1191
Quantity1ml
GenotypeF-, ompT, hsdS_B (r_B –m_B –), dcm, gal, (DE3), pLysS, Cm^r
Cell Type (standardized)Competent Cells
Validated Species ReactivityEscherichia

Specifications/Features

Transformation Efficiency:
10⁶cfu/µg

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) GPLD1 is a scavenger carrier mediating lysosomal degradation of extracellular aberrant proteinsLife Sci AllianceJune 5, 2026Mizuki Tsuchiya, Yoichiro Yagishita, Eisuke Itakuraand Ab42-ALFA-His were cloned into the pRSET-A vector and expressed in E. coli BL21(DE3) pLysS (cat# L1191; Promega). After harvesting, E. coli cells were disrupted by sonication. After ultracentrifugation at

(2) Regulatory dynamics of Sch9 in response to cytosolic acidification: From spatial reconfiguration to cellular adaptation to stressesiScienceJanuary 17, 2025Rui Fujii, Rai Katsukawa, Eigo Takeda, Eisuke Itakura, Akira Matsuuravirus strains,Bacterial and virus strains,Bacterial and virus strains ,, BL21(DE3)pLysS,Promega,Cat# L1191 ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(3) A cryptic K48 ubiquitin chain binding site on UCH37 is required for its role in proteasomal degradationeLifeApril 22, 2022Du J, Babik S, Li Y, Deol KK, Eyles SJ, Fejzo J, Tonelli M, Strieter E.strains Rosetta 2(DE3)pLysS (EMD Millipore Novagen, Cat. # 71403–3)BL21(DE3)pLysS (Promega, Cat. # L1191)One Shot Stbl3 (Fisher Scientific, Cat. # C737303) Chemicals, peptides, and recombinant proteins ProBlock

(4) CrvA and CrvB form a curvature-inducing module sufficient to induce cell-shape complexity in Gram-negative bacteriaNature microbiologyJuly 1, 2021Martin NR, Blackman E, Bratton BP, Chase KJ, Bartlett TM, Gitai Z.cleavage site. The plasmid was transformed by electroporation into E. coli BL21 cells (Promega, cat# L1191). Overnight cultures were back-diluted 1:100 into LB containing 100μg/mL carbenicillin and grown at

(5) Proteasome-Bound UCH37/UCHL5 Debranches Ubiquitin Chains to Promote DegradationMolecular cellDecember 3, 2020Deol KK, Crowe SO, Du J, Bisbee HA, Guenette RG, Strieter ER.Strains,Bacterial and Virus Strains Rosetta 2(DE3)pLysS,EMD Millipore Novagen,71403-3 BL21(DE3)pLysS,Promega,L1191 One Shot™ TOP10,Fisher Scientific,C404003 One Shot™ Stbl3,Fisher Scientific,C737303 "Chemicals, Peptides,

(6) Biased localization of actin binding proteins by actin filament conformationNature CommunicationsDecember 1, 2020Andrew R. Harris, Pamela Jreij, Brian Belardi, Aaron M. Joffe, Andreas R. Bausch, Daniel A. Fletcher1–261) and its associated mutants were expressed recombinantly in E. coli BL21 (DE3) pLysS (Promega, #L1191). Cells were lysed by sonication and HIS tagged protein containing a SUMO solubility tag were purified

(7) Monoclonal antibody against H1N1 influenza virus hemagglutinin cross reacts with hnRNPA1 and hnRNPA2/B1Molecular medicine reportsNovember 1, 2020Guo C, Sun L, Hao S, Huang X, Hu H, Liang D, Feng Q, Li Y, Feng Y, Xie X, et al.YB-ATCC-2224). BL21(DE3)pLysS competent cells, >106 cfu/µg, were purchased from Promega Corporation (cat. no. L1191). Protein A/G PLUS agarose (cat. no. GS4780) was purchased from Santa Cruz Biotechnology, Inc. The total

(8) Steric regulation of tandem calponin homology domain actin-binding affinityMol. Biol. CellDecember 15, 2019Andrew R. Harris, Brian Belardi, Pamela Jreij, Kathy Wei, Hengameh Shams, Andreas Bausch, Daniel A. Fletcherits associated mutants were expressed recombinantly in Escherichia coli BL21 (DE3) pLysS (Promega, #L1191) and purified using affinity chromatography followed by gel filtration. Proteins were stored in 20 mM

(9) Steric regulation of tandem calponin homology domain actin-binding affinityMolecular Biology of the CellDecember 15, 2019Andrew R. Harris, Brian Belardi, Pamela Jreij, Kathy Wei, Hengameh Shams, Andreas Bausch, Daniel A. Fletcherits associated mutants were expressed recombinantly in Escherichia coli BL21 (DE3) pLysS (Promega, #L1191) and purified using affinity chromatography followed by gel filtration. Proteins were stored in 20 mM

(10) Significance of a histone-like protein with its native structure for the diagnosis of asymptomatic tuberculosisPLOS ONEOctober 25, 2018Yukiko Ohara et al.the National Institute of Genetics, Japan. Recombinant protein preparation E. coli BL21 (DE3) pLysS (L1191, Promega, Madison, WI, USA) cells were transformed with the previously constructed plasmid that expresses

(11) Plasma Membrane Localization of Apoptotic Caspases for Non-apoptotic FunctionsDevelopmental cellMay 21, 2018Amcheslavsky A, Wang S, Fogarty CE, Lindblad JL, Fan Y, Bergmann A.Strains,Bacterial and Virus Strains,Bacterial and Virus Strains E. coli strain BL21pLysS(DE3),Promega,Cat#L1191 "Chemicals, Peptides, and Recombinant Proteins","Chemicals, Peptides, and Recombinant Proteins","Chemicals,

(12) The IRF4 Gene Regulatory Module Functions as a Read-Write Integrator to Dynamically Coordinate T Helper Cell FateImmunitySeptember 6, 2017Veena Krishnamoorthy, Sunil Kannanganat, Mark Maienschein-Cline, Sarah L. Cook, Jianjun Chen, Neil Bahroos, Evelyn Sievert, Emily Corse, Anita Chong, Roger Sciammas,Experimental Models: Cell LInes Mammalian: P13,"Yamane et al., 2005",N/A Bacterial: BL21 Rosetta (DE3),Promega,L1191 Bacterial: NEB® 5-alpha,New England Biolabs,C2988J Experimental Models: Organisms/Strains,Experimental Models

(13) An inhibitory mono-ubiquitylation of the Drosophila initiator caspase Dronc functions in both apoptotic and non-apoptotic pathwaysPLoS geneticsFebruary 1, 2017Kamber Kaya HE, Ditzel M, Meier P, Bergmann A.6xHis fusion proteins. Generated plasmids were transformed to BL21(DE3)pLysS competent cells (Promega L1191). 50 ul of bacterial culture was grown at 37˚C. Plasmid expression was induced by 0.2 mM IPTG for 3

(14) GLTP Mediated Non-Vesicular GM1 Transport between Native MembranesPLoS ONEMarch 28, 2013Ines Lauria, Jan van Üüm, Esmina Mjumjunov-Crncevic, David Walrafen, Luis Spitta, Christoph Thiele, Thorsten Langexpression vector via the NdeI and BamHI restriction sites. After transformation into BL21(DE3)pLysS (Cat# L1191, Promega) soluble GLTP and GLTP mutants were expressed after induction with 10 mM IPTG for 6 h at 24°C.

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