dam-/dcm- Competent E. coli from New England Biolabs

Product Specs
Itemdam-/dcm- Competent E. coli
CompanyNew England Biolabs
Price $206.00 Inquire
Catalog NumberC2925I
Quantity6 x 0.2 ml
Genotypeara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10) Tet^S endA1 rspL136 (Str^R) dam13::Tn9 (Cam^R) xylA-5 mtl-1 thi-1 mcrB1 hsdR2
ApplicationsDNA Manipulation
Cell Type (standardized)Competent Cells
Validated Species ReactivityEscherichia
SpeciesE. coli
Temperature-80 C
Cell TypeCompetent Cells

Specifications/Features

Transformation Efficiency:
1-3 x 10⁶ cfu/µg

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) FACT weakens the nucleosomal barrier to transcription and preserves its integrity by forming a hexasome-like intermediateMolecular CellJune 5, 2025Francesca Burgos-Bravo, Alexander B. Tong, Chen Li, César Díaz-Celis, Craig D. Kaplan, Gary LeRoy, Danny Reinberg, Carlos BustamanteRESOURCE,SOURCE,IDENTIFIER Bacterial strains,Bacterial strains,Bacterial strains dam-/dcm- Competent E. coli,New England Biolabs,Cat# C2925I BL21 Competent E. coli,"Macrolab, QB3-Berkeley",N/A BJ5464 Rpb3 His-Bio S. cerevisiae,Kireeva et al.50,N/A Cell

(2) MinD2 modulates cell shape and motility in the archaeon Haloferax volcaniiFrontiers in MicrobiologyNovember 12, 2024Megha Patro, Felix Grünberger, Shamphavi Sivabalasarma, Sabrina Gfrerer, Marta Rodriguez-Franco, Phillip Nußbaum, Dina Grohmann, Solenne Ithurbide, Sonja-Verena AlbersFor transformation into H. volcanii, non-methylated plasmids were extracted from E. coli dam−/dcm− (C2925I, NEB). Mutant strains generated and used are described in Supplementary Table 1. Plasmids created for

(3) Optimising Transformation Efficiency in Borrelia: Unravelling the Role of the Restriction-Modification System of Borrelia afzelii and Borrelia gariniiInternational Journal of Molecular SciencesOctober 22, 2024Margarida Ruivo, Noémi Zsuzsa Kovács, Anna-Margarita Schötta, Theresa Stelzer, Laura Hermann, Verena Mündler, Andreas Bergthaler, Michael Reiter, Michiel Wijnveld(Microsynth Austria GmbH). Finally, all obtained plasmids were used to transform a dam-/dcm- E. coli strain (C2925I, New England Biolabs) and plated out on ampicillin-containing LB agar plates (50 µg/mL). 4.5. Gene Expression

(4) Functional divergence of a bacterial enzyme promotes healthy or acneic skinNat CommunDecember 5, 2023Irshad A. Hajam, Madhusudhanarao Katiki, Randall McNally, María Lázaro-Díez, Stacey Kolar, Avradip Chatterjee, Cesia Gonzalez, Mousumi Paulchakrabarti, Biswa Choudhury, J. R. Caldera, et al.purified and verified by PCR. Correct plasmids were transformed into dam-negative E. coli (Catalog # C2925I, New England Biolabs) and purified. Competent C. acnes cells were prepared as described previously37.

(5) Examining chromatin heterogeneity through PacBio long-read sequencing of M.EcoGII methylated genomes: an m 6 A detection efficiency and calling bias correcting pipelinebioRxivNovember 30, 2023Allison F. Dennis, Zhuwei Xu, David J. Clarkin pUC19 methylated by Dam or M.EcoGII pUC19 was purified from a dam- dcm- strain of E. coli (NEB C2925I) to prevent endogenous DNA methylation, and linearised with SmaI. Three 350-μl reactions were prepared

(6) “Influence of plasmids, selection markers and auxotrophic mutations on Haloferax volcanii cell shape plasticity”Frontiers in MicrobiologySeptember 29, 2023Megha Patro, Iain G. Duggin, Sonja-Verena Albers, Solenne Ithurbideon the transforming plasmid. Non-methylated plasmids were extracted from Escherichia coli dam−/dcm− (C2925I, NEB) prior to transformation of H. volcanii. E. coli strains were cultured in LB medium, with the necessary

(7) A role for N6-methyldeoxyadenosine in C. elegans mitochondrial genome regulationbioRxivMay 17, 2023Lantana K. Grub, James P. Held, Tyler J. Hansen, Samantha H. Schaffner, Marleigh R. Canter, Evi M. Malagise, Maulik R. Patelvirus strains OP50 E. Coli (Uracil auxotroph),CGC, NA22 E. Coli,CGC, dam-/dcm- competent E. Coli,NEB,C2925I ,, ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(8) Recombineering in Non‐Model BacteriaCurrent ProtocolsDecember 22, 2022Anna Corts, Lynn C. Thomason, Nina Costantino, Donald L. CourtLaboratory). Chemically competent dam− dcm−E. coli cells from New England Biolabs (NEB) (https://www.neb.com) Catalog # C2925I. Alternatively, obtain GM2163 (CGSC#6581, chloramphenicol resistant) and GM1674 (CGSC#7971) from the

(9) Molecular organization of the early stages of nucleosome phase separation visualized by cryo-electron tomographyMolecular CellAugust 18, 2022Meng Zhang, César Díaz-Celis, Bibiana Onoa, Cristhian Cañari-Chumpitaz, Katherinne I. Requejo, Jianfang Liu, Michael Vien, Eva Nogales, Gang Ren, Carlos BustamanteRESOURCE,SOURCE,IDENTIFIER Bacterial strains,Bacterial strains,Bacterial strains dam-/dcm- Competent E. coli,New England Biolabs,C2925I DH5α E. coli competent cells,"Macrolab, QB3-Berkeley",N/A BL21(DE3) pLysS E. coli competent cells,"Macrolab,

(10) Multiplexed Assembly and Annotation of Synthetic Biology Constructs Using Long-Read Nanopore SequencingACS Synthetic BiologyJuly 15, 2022Francesco E. Emiliani, Ian Hsu, Aaron McKennaspecifications. Demethylated Plasmid Preparation Plasmids were transformed into NEB dam–/dcm– competent E. coli (C2925I) as per the vendor’s protocols and plated onto LB agar with ampicillin. The following day we picked

(11) A broad-spectrum cloning vector that exists as both an integrated element and a free plasmid in Chlamydia trachomatisPLOS ONEDecember 16, 2021Lotisha Garvin, Rebecca Vande Voorde, Mary Dickinson, Steven Carrell, Kevin Hybiske, Daniel Rockeyelements, was designated pBVR2 (S1 Fig), and transformed into E. coli dam-/dcm- competent cells (NEB C2925I), grown in 250 ml of LB broth plus 100 μg/ml Amp for plasmid extraction and purified using a Plasmid

(12) An inducible transposon mutagenesis approach for the intracellular human pathogen Chlamydia trachomatisWellcome Open ResearchNovember 16, 2021Colette E. O'Neill, Rachel J. Skilton, Jade Forster, David W. Cleary, Sarah A. Pearson, David J. Lampe, Nicholas R. Thomson, Ian N. ClarkeE. coli,C2925,Methyltransferase deficient (dam-/dcm-) chemically competent E. coli,Supplied by New England Biolabs (C2925I) C. trachomatis,SWFP-,"Plasmid-free urogenital strain, serovar F.","Wang et al., 2013 24 . Supplied

(13) CpG Methylation Profiles of HIV-1 Pro-Viral DNA in Individuals on ARTVirusesApril 29, 2021Valerie F. Boltz et al.and harvesting of the plasmid from a methyltransferase deficient (Dam-/Dcm-) strain of E. coli (NEB; #C2925I). To generate the methylated, positive control template, 1 × 106 CEM cells were spiked with approximately 50

(14) Characterization of Cell Free Plasma Methyl-DNA From Xenografted Tumors to Guide the Selection of Diagnostic Markers for Early-Stage CancersFrontiers in OncologyMarch 26, 2021Ling Liu et al.mice (2–12.8 ng mouse cfDNA each) were used, together with methyltransferase deficient E. coli [NEB C2925I, genotype: ara14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm6 hisG4 rfbD1 R (zgb210::Tn10)

(15) Discovery of an Unnatural DNA Modification Derived from a Natural Secondary MetaboliteCell Chemical BiologyJanuary 1, 2021Tong Wang, Rahul M. Kohli(Figure S1A–C) (Baba et al., 2006; Miller, 1992). For Dcm experiments, dcm− /dam− E. coli were used (NEB C2925I, ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10) TetS endA1

(16) μDamID: A Microfluidic Approach for Joint Imaging and Sequencing of Protein-DNA Interactions in Single CellsCell SystemsOctober 1, 2020Nicolas Altemose, Annie Maslan, Carolina Rios-Martinez, Andre Lai, Jonathan A. White, Aaron Streetsand Virus Strains,Bacterial and Virus Strains Dam−/dcm− Competent E. Coli,New England Biolabs,Cat#C2925I "Chemicals, Peptides, and Recombinant Proteins","Chemicals, Peptides, and Recombinant Proteins","Chemicals,

(17) The acute myeloid leukemia variant DNMT3A Arg882His is a DNMT3B-like enzymeNucleic Acids ResearchApril 17, 2020Allison B Norvil, Lama AlAbdi, Bigang Liu, Yu Han Tu, Nicole E Forstoffer, Amie R Michie, Taiping Chen, Humaira Gowherperformed using 100 ng of an unmethylated pUC19 plasmid purified from dam− /dcm−Escherichia coli strain (C2925I, NEB) or a 1-kb fragment containing 14 CpG sites amplified from the Meis1 enhancer were used as DNA

(18) Simple CRISPR‐Cas9 Genome Editing in Saccharomyces cerevisiaeCurrent Protocols in Molecular BiologyDecember 1, 2019Marian F. Laughery, John J. Wyrickvector Transform sgRNA expression vector (e.g., pML104 or pML107) into a dam- E. coli strain (e.g., NEB #C2925I; see Current Protocols article; Seidman et al., 1997) and isolate plasmid DNA from dam- E. coli strain

(19) Genome-wide CRISPR Screens in T Helper Cells Reveal Pervasive Crosstalk between Activation and DifferentiationCellFebruary 7, 2019Henriksson J, Chen X, Gomes T, Ullah U, Meyer KB, Miragaia R, Duddy G, Pramanik J, Yusa K, Lahesmaa R, et al.kosuke_mfei_fwd/kosuke_clai_rev). The plasmid pMSCV-IRES-mCherry FP (Addgene #52114) grown in dam-/dcm- competent E. coli (NEB #C2925I), was digested with NEB ClaI/MfeI and the backbone was gel purified using the QIAquick Gel Extraction

(20) A Nucleosome Bridging Mechanism for Activation of a Maintenance DNA MethyltransferaseMolecular CellJanuary 1, 2019Caitlin I. Stoddard, Suhua Feng, Melody G. Campbell, Wanlu Liu, Haifeng Wang, Xuehua Zhong, Yana Bernatavichute, Yifan Cheng, Steven E. Jacobsen, Geeta J. NarlikarStrains,Bacterial and Virus Strains Rosetta (DE3),Novagen,70954 BL21 (DE3) pLysS,Promega,L1195 Dam-/dcm-,NEB,C2925I "Chemicals, Peptides, and Recombinant Proteins","Chemicals, Peptides, and Recombinant Proteins","Chemicals,

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