T7 Express lysY/Iq Competent E. coli (High Efficiency) from New England Biolabs

Product Specs
ItemT7 Express lysY/Iq Competent E. coli (High Efficiency)
CompanyNew England Biolabs
Price $190.00 Inquire
Catalog NumberC3013I
GenotypefhuA2 lacZ::T7 gene1 [lon] ompT gal sulA11 R(mcr-73::miniTn10--Tet^S)2 [dcm] R(zgb-210::Tn10--Tet^S) endA1 Δ(mcrC-mrr)114::IS10 [miniF-lysY, lacI^q(Cam^R)]
Quantity6 x 0.2 ml
ApplicationsProteomics & Glycomics
Cell Type (standardized)Competent Cells
Validated Species ReactivityEscherichia
SpeciesE. coli
Temperature-80 C
Cell TypeCompetent Cells

Specifications/Features

Transformation Efficiency:
2 - 6 x 10⁸ cfu/µg

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Discovery of BilV reveals a multienzymatic basis for bilirubin reduction across vertebrate gut microbiomesbioRxivJune 2, 2026Angela K. Jiang, Maggie R. Grant, Gabriela Arp, Keith Dufault-Thompson, Alexandra M. Clarke, Yue Li, Danielle Lehman, Alan K. Jarmusch, Brantley Hall, Xiaofang Jiangvector. Both constructs were transformed into T7 Express lysY/Iq Competent E. coli (New England Biolabs, C3013I) and verified by Oxford Nanopore sequencing (Plasmidsaurus). For whole-cell biotransformation, transformed

(2) Structural and mechanistic insights into the constitutive Themis–Grb2 complex in T cell signallingNature CommunicationsMay 20, 2026Danielle M. Clancy, Alba Sanz-Sanjuan, Elisabeth Gilis, Peter Tougaard, Imke Velghe, Yana Van Droogenbroeck, Jan Felix, Yehudi Bloch, Álvaro Furones Cuadrado, Romain Merceron, et al.by an N-terminal His6 tag and a caspase-3 cleavage site. BL21 T7 Express lysY/Iq (NEB, catalogue no. C3013I) were transformed with pET15.Nb256 and protein expression were induced at OD600 0.6–0.9 with 1 mM IPTG

(3) CoCUT&Tag maps linked chromatin states at single-molecule, single-cell resolutionbioRxivMay 5, 2026Wenjie Sun, Emma Baird, Harry J. Ashbaugh, Alexandria P. Eiken, Derek H. Janssenspurification workflow. Each construct was transformed into T7 Express lysY/Iq Competent E. coli (NEB #C3013I) following the manufacturer’s protocol and plated for selective overnight growth. A single colony was

(4) SpiR is a gut microbial enzyme that drives cholesterol conversionNat CommunApril 14, 2026Gabriela Arp, Sophia Levy, Angela K. Jiang, Keith Dufault-Thompson, Aoshu Zhong, Maggie Grant, Yue Li, Xiaofang Jiang, Brantley HallPlasmids were individually transformed into T7 Express lysY/Iq Competent E. coli (New England Biolabs, C3013I) using the manufacturer’s protocol. The cells were plated on Luria-Bertani (LB, Sigma-Aldrich, L3022)

(5) DiMeLo-cito: a one-tube protocol for mapping protein-DNA interactions reveals CTCF bookmarking in mitosisbioRxivApril 11, 2026Nathan Gamarra, Cy Chittenden, Kousik Sundararajan, Jacob P. Schwartz, Sofia Lundqvist, Denise Robles, Oberon Dixon-Luinenburg, Jeremy Marcus, Annie Maslan, J. Matthew Franklin, et al.targeting mouse IgG Fc were used for anti-rabbit and anti-mouse Hia5 respectively. T7 Express lysY/Iq (NEB C3013I) cells were transformed with expression plasmid according to manufacturer specifications and plated

(6) Cloneable contrast across all biological length scalesJournal of Structural BiologyMarch 1, 2026Kanda M. Borgognoni, Bradley F. Guilliams, Zachary J. Butz, Christopher J. Ackerson(bracketed with red) for the SeNPs. M9 – Materials T7 Expression lysY/Iq Competent E. coli cells (Cat no. C3013I) were purchased from New England Biolabs, Inc. Original plasmids containing GRLMR and F-cSeNP-His were

(7) Simplified MS2 phage-like particle production including a novel maturation protein internal fusion affinity tagBiotechnology & Biotechnological EquipmentDecember 31, 2025Enos C. Kline, Rose Duong, Qin Wang, Barry R. Lutzlysis Plasmids were transformed into T7 Express lysY/Iq Competent E. coli (High Efficiency) (NEB, C3013I) per the manufacturer’s protocol. Select clones were sequence-verified by Sanger sequencing or whole

(8) Staphylococcus aureus exploits lipoic acid salvage to combat host oxidative stressCell ReportsAugust 26, 2025Iván C. Acosta, Andrew Albers, Liwei Fang, Gustavo Serrato, Wei Ping Teoh, David G. Glanville, Francis Alonzo,New England Biolabs,Cat #C2989K IM08B E. coli,Monk et al.95,N/A lysY/Iq E. coli,New England Biolabs,Cat #C3013I lysY/Iq ΔlipA::kan,Grayczyk et al.21,FA-E1344 E. coli lysY/Iq ΔlipA::kan + pET15b-6xhis-gcvH-L,Laczkovich

(9) Protein-based tools for the detection and characterisation of Oropouche virus infectionEMBO Molecular MedicineAugust 11, 2025Monique K Merchant, Juliano de Paula Souza, Sahar Abdelkarim, Shrestha Chakraborty, Tufi A Nasser Neto, Kristel I Gutierrez Manchay, Daniel M de Melo Jorge, Valdinete Alves do Nascimento, Yuxian Sun, Eve R Caroe, et al.CCL-2 Vero cells,ATCC,Cat# CCL-81 Escherichia coli T7 Express lysY/Iq cells,New England Biolabs,Cat# C3013I Escherichia coli WK6 cells,ATCC,Cat# 47078 Oropouche virus strain BeAn19991,Aquino et al (2003) 10.1007/s00705-002-0913-4, Oropouche

(10) Combinatorial profiling of multiple histone modifications and transcriptome in single cells using scMTR-seqScience advancesAugust 8, 2025Wang Y, Li J, Malcolm AA, Mansfield W, Clark SJ, Argelaguet R, Biggins L, Acton RJ, Andrews S, Reik W, et al.(45) was transformed into T7 Express lysY/I q Competent Escherichia coli [New England Biolabs (NEB), C3013I]. A single colony was picked and propagated in 3 ml LB medium at 220 rpm, 37°C for 4 hours as a starter

(11) Chromatin architecture mapping by multiplex proximity taggingMolecular CellJuly 17, 2025Axel Delamarre, Benton Bailey, Jennifer Yavid, Richard Koche, Neeman Mohibullah, Iestyn Whitehouseactivation pETDuet_huCAD_ICADL (Addgene, 100098) was transformed into T7 Express LysY/Iq competent cells (NEB, C3013I). Cells were grown in 2.4L of LB2YT (Tryptone 10 g/L, Yeast Extract 10 g/L, NaCl 5 g/L) until OD reached

(12) Identification of gut bacteria reductases that biotransform steroid hormonesNat CommunJuly 8, 2025Gabriela Arp, Angela K. Jiang, Keith Dufault-Thompson, Sophia Levy, Aoshu Zhong, Jyotsna Talreja Wassan, Maggie R. Grant, Yue Li, Brantley Hall, Xiaofang Jiangmanufacturer’s instructions: NEB 10-beta (NEB, C3019) for pCW-lic plasmids and NEB T7 Express lysY/Iq (NEB, C3013I) for pET28a(+) and pET11 vectors. Transformed cells were plated on Luria-Bertani (LB) agar containing

(13) A distinct class of ferredoxin:NADP+ oxidoreductase enzymes driving thermophilic ethanol productionJournal of Biological ChemistryJuly 1, 2025Shu Huang, Syed Muhammad Saad Imran, Anthony A. Lanahan, Sarah K. Hammer, Carolyn E. Lubner, Lee R. Lynd, Daniel G. Olsonhas NADH-linked ALDH activity and NADPH-linked ADH activity. E. coli T7 Express lysY/Iq (NEB catalog #C3013I) was used for the overexpression of Fnor enzymes for purification. E. coli T7 Express (NEB catalog #C2566H)

(14) Evolutionary paths that link orthogonal pairs of binding proteinsCell SystemsMay 21, 2025Ziv Avizemer, Carlos Martí Martí-Gómez, Shlomo Yakir Hoch, David M. McCandlish, Sarel J. Fleishmanstrains pET21d,Netzer el al.30,http://www.addgene.org/107127 E.coli T7 Express lysY∕Iq,New England Biolabs,NEB C3013I E. coli JM83,Addgene,Cat# 50348 Softwares and algorithms,Softwares and algorithms,Softwares and algorithms Atomistic

(15) Combining Multiplexed CRISPR/Cas9-Nickase and PARP Inhibitors Efficiently and Precisely Targets Cancer CellsCancer ResMay 6, 2025Soyoung Lee, Kyunghwan Kim, Hye-Jin Jeong, Subin Choi, Himchan Cheng, Dayoung Kim, Soomin Heo, Jinhee Mun, Minjong Kim, Eunjin Lee, et al.(Addgene, #104171, RRID: Addgene_104171). These vectors were transformed into the C3013 strain (NEB, #C3013I), and recombinant Cas9WT or LbCas12a-ultra protein was expressed at 25°C for 4 hours by addition of

(16) Functional characterization of the ATOH1 molecular subtype indicates a pro-metastatic role in small cell lung cancerCell ReportsMay 1, 2025Alessia Catozzi, Maria Peiris Pagès, Sam Humphrey, Mitchell Revill, Derrick Morgan, Jordan Roebuck, Yitao Chen, Bethan Davies-Williams, Kevin Brennan, A.S. Md. Mukarram Hossain, et al.50 ng PET28A-ATOH1 were transformed in T7 Express lysY/Iq Competent E. coli (High Efficiency) (NEB, C3013I) according to manufacturer’s instructions. The next day, colonies were picked and cultured in 5–10 mL

(17) Iron-Dependent JMJD1A-Mediated Demethylation of H3K9me2 Regulates Gene Expression During Adipogenesis in a Spatial Genome Organization-Dependent MannerGenes CellsApril 28, 2025Shinnosuke Masuda, Tetsuro Komatsu, Safiya Atia, Tomohiro Suzuki, Mayuko Hayashi, Atsushi Toyoda, Hiroshi Kimura, Takeshi Inagaki3XFlag‐pA‐Tn5‐FI (Addgene, 124,601) was transformed into T7 Express lysY/Iq competent E. coli (NEB, C3013I), and transformants were grown overnight on LB agar plates containing 100 μg/mL ampicillin. A single

(18) Improved precision, sensitivity, and adaptability of ordered two-template relay cDNA library preparation for RNA sequencingRNAFebruary 1, 2025Lucas Ferguson, Heather E. Upton, Sydney C. Pimentel, Chris Jeans, Nicholas T. Ingolia, Kathleen Collinspossesses a plasmid to overexpress rare E. coli tRNAs, or T7 Express LysY/Iq (New England Biolabs, C3013I). Cells were transformed with an expression plasmid (e.g., Addgene plasmid 186461, 185710, or 185713)

(19) Artificial cells with all-aqueous droplet-in-droplet structures for spatially separated transcription and translationNat CommunJanuary 24, 2025Kanji Tomohara, Yoshihiro Minagawa, Hiroyuki NojipRSET-B vector with an N-terminal His-rich tag, expressed in T7 Express lysY/Iq Competent E. coli (NEB, C3013I) and purified as previously reported with some modifications25. Fresh colonies on LB agar plate with

(20) Single chromatin fiber profiling and nucleosome position mapping in the human brainCell Reports MethodsDecember 16, 2024Cyril J. Peter, Aman Agarwal, Risa Watanabe, Bibi S. Kassim, Xuedi Wang, Tova Y. Lambert, Behnam Javidfar, Viviana Evans, Travis Dawson, Maya Fridrikh, et al.S2A). We empirically determined the optimal expression and production of Hia5 in E.coli T7 express (NEB, #C3013I), OD600 0.8 at 18oC, with 0.5 mM IPTG (Invitrogen, AM9464) for induction, and repeated 6-hour spins

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