T7 ExpresslysYCompetentE. coli(High Efficiency) from New England Biolabs

Product Specs
ItemT7 ExpresslysYCompetentE. coli(High Efficiency)
CompanyNew England Biolabs
Price $190.00 Inquire
Catalog NumberC3010I
Quantity6 x 0.2 ml
GenotypefhuA2 lacZ::T7 gene1 [lon] ompT gal sulA11 R(mcr-73::miniTn10--Tet^S)2 [dcm] R(zgb-210::Tn10--Tet^S) endA1 Δ(mcrC-mrr)114::IS10 [miniF-lysY (Cam^R)]
ApplicationsProteomics & Glycomics
Cell Type (standardized)Competent Cells
Validated Species ReactivityEscherichia
SpeciesE. coli
Temperature-80 C
Cell TypeCompetent Cells

Specifications/Features

Transformation Efficiency:
2 - 6 x 10⁸ cfu/µg

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Bacterial Schlafen proteins mediate phage defenceNature MicrobiologyMarch 9, 2026Veronica Perez Taboada, Yimo Wu, Riley Cassidy, Kirill E. Medvedev, Luuk Loeff, Anna Nemudraia, Artem Nemudryi(6xHisTwinStrepSUMO-RorSlfn5) and pAN302 (6xHisTwinStrepSUMO-T5.142) were transformed into T7 Express lysY cells (NEB, C3010I). Cells were grown in LB broth (Lennox) supplemented with ampicillin (100 µg ml−1) at 37 °C to an OD600

(2) Oligomerization enables the selective targeting of an intrinsically disordered region by a small moleculeScience AdvancesFebruary 27, 2026Stasė Bielskutė-García, Borja Mateos, Muhammad Awawdy, Carla Garcia-Cabau, Henri Niskanen, Carolina Sánchez-Zarzalejo, Lorenzo Bracaglia, Roberta Pierattelli, Isabella C. Felli, Marta Frigolé-Vivas, et al.+ H434A + F437A) mutants of Tau-5* were produced in E. coli BL21 (DE3) LysY (New England Biolabs, #C3010I) cells at 25°C overnight for unlabeled LB media and 37°C for 3 hours for 15N/13C M9 media. In all cases,

(3) Structural basis of undecaprenyl phosphate glycosylation leading to polymyxin resistance in Gram-negative bacteriaNat CommunDecember 9, 2025Khuram U. Ashraf, Mariana Bunoro-Batista, T. Bertie Ansell, Ankita Punetha, Stephannie Rosario-Garrido, Emre Firlar, Jason T. Kaelber, Phillip J. Stansfeld, Vasileios I. Petroupurification The resulting ArnC constructs were transformed into T7 Express lysY E. coli competent cells (NEB, C3010I), an enhanced BL21 derivative strain, and grown overnight in 1 mL 2xYT medium supplemented with 50 μg/mL

(4) Structural basis of undecaprenyl phosphate glycosylation leading to polymyxin resistance in Gram-negative bacteriabioRxivFebruary 1, 2025Khuram U. Ashraf, Mariana Bunoro-Batista, T. Bertie Ansell, Ankita Punetha, Stephannie Rosario-Garrido, Emre Firlar, Jason T. Kaelber, Phillip J. Stansfeld, Vasileios I. Petroupurification. The resulting ArnC constructs were transformed into T7 Express lysY E. coli competent cells (NEB, C3010I), an enhanced BL21 derivative strain, and grown overnight in 1mL 2xYT medium supplemented with 50μg/mL

(5) Structure of the human autophagy factor EPG5 and the molecular basis of its conserved mode of interaction with Atg8-family proteinsAutophagyJanuary 14, 2025Yiu Wing Sunny Cheung, Sung-Eun Nam, Gage M. J. Fairlie, Karlton Scheu, Jennifer M. Bui, Hannah R. Shariati, Jörg Gsponer, Calvin K. Yipas an N-terminally GST-tagged construct in Escherichia coli T7 Express cells (New England Biolabs, C3010I). Cells were grown in minimal M9 media supplemented with 1 g/L 15NH4Cl (Cambridge Isotope Laboratories,

(6) Laboratory Evolution of Metalloid Reductase Substrate Recognition and Nanoparticle Product SizeACS Chemical BiologyFebruary 16, 2024Alexander R. Hendricks, Rachel S. Cohen, Gavin A. McEwen, Tony Tien, Bradley F. Guilliams, Audrey Alspach, Christopher D. Snow, Christopher J. AckersonGRLMR mutants. The library was subsequently transformed into T7 Express lysY (New England BioLabs #C3010I) for downstream selection experiments. To validate the mutational load of our WT-GRLMR DNA library,

(7) CasPlay provides a gRNA-barcoded CRISPR-based display platform for antibody repertoire profilingCell Rep MethodsOctober 24, 2022Barber KW, Shrock E, Elledge SJ.strains,Bacterial and virus strains,Bacterial and virus strains ,, T7 Express lysY Competent E. coli,NEB,C3010I Electromax DH10B E. coli,ThermoFisher,18290015 ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, SurePrint

(8) Human RNase 4 improves mRNA sequence characterization by LCâ€“MS/MSNucleic Acids ResearchOctober 14, 2022Eric J Wolf, Sebastian GrÃ¼nberg, Nan Dai, Tien-Hao Chen, Bijoyita Roy, Erbay Yigit, IvanÂ R CorrÃªagal sulA11 R(mcr-73::miniTn10–TetS)2 [dcm] R(zgb-210::Tn10–TetS) endA1 Δ(mcrC-mrr)114::IS10] (NEB #C3010I) for 16 h at 16°C. The cells were lysed by sonication in lysis buffer (20 mM Tris–HCl pH 7.5, 200 mM

(9) Understanding the separation of timescales in bacterial proteasome core particle assemblyBiophysical JournalOctober 1, 2022Pushpa Itagi, Anupama Kante, Leonila Lagunes, Eric J. DeedsUSA). Plasmids were freshly transformed in E. coli BL21 (DE3)-pLyse S (NEB, Ipswich, MA, USA, catalog #C3010I) cells. Cells were grown in 4 L of Luria Barteni media containing 100 μg/mL carbenicillin and 30 μg/mL

(10) Preparation and Use of Cellular Reagents: A Low‐resource Molecular Biology Reagent PlatformCurrent ProtocolsApril 1, 2022Sanchita Bhadra, Inyup Paik, Jose‐Angel Torres, Stéphane Fadanka, Chiara Gandini, Harry Akligoh, Jenny Molloy, Andrew D. Ellingtonperformed using low-copy plasmids and engineered E. coli strains (e.g., T7 Express lysY, NEB, cat. No. C3010I) designed to reduce leaky expression. Most commonly used protein expression systems can, in turn, be

(11) The stem rust effector protein AvrSr50 escapes Sr50 recognition by a substitution in a single surface‐exposed residueNew PhytologistApril 1, 2022Diana Ortiz et al.and protein purification AvrSr50 proteins were expressed in T7 Express lysY competent E. coli (NEB, C3010I). Bacterial cultures were grown in Terrific Broth (TB) medium at 37°C with shaking at 220 RPM until

(12) Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of nsp14/nsp10 exoribonucleaseBiochemical JournalJuly 16, 2021Berta Canal et al.169161). For expression of the nsp10–nsp14 fusion protein, T7 Express LysY competent cells from NEB (C3010I) were transformed with plasmid 169161 (Supplementary Table S1) and grown overnight (ON) at 37°C in LB

(13) Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of nsp14/nsp10 exoribonucleaseBiochem JMay 11, 2021Berta Canal, Allison W. McClure, Joseph F. Curran, Mary Wu, Rachel Ulferts, Florian Weissmann, Jingkun Zeng, Agustina P. Bertolin, Jennifer C. Milligan, Souradeep Basu, et al.169161). For expression of the nsp10–nsp14 fusion protein, T7 Express LysY competent cells from NEB (C3010I) were transformed with plasmid 169161 (Supplementary Table S1) and grown overnight (ON) at 37°C in LB

(14) FRET-based Microscopy Assay to Measure Activity of Membrane Amino Acid Transporters with Single-transporter ResolutionBIO-PROTOCOLApril 5, 2021Didar Ciftci, Gerard H M Huysmans, Xiaoyu Wang, Changhao He, Daniel Terry, Zhou Zhou, Gabriel Fitzgerald, Scott C Blanchard, Olga Boudker27104) 45. T7 express lysY competent E. coli (High efficiency) (New England Biolabs, catalog number: C3010I) 46. Thermo Scientific TM FastDigest DpnI (Fisher Scientific, catalog number: FERFD1704) 47. PfuUltra II Hotstart PCR master mix

(15) Atypical UV Photoproducts Induce Non-canonical Mutation Classes Associated with Driver Mutations in MelanomaCell ReportsNovember 17, 2020Marian F. Laughery et al.10XHis tagged CPD photolyase was sequence confirmed and transformed into T7 Express lysY E. coli (NEB, C3010I). To avoid bacteria entering stationary phase and reducing phrB expression, a single colony containing

(16) Using Yeast as a Model Organism to Study the Functional Roles of Histone Acetylation in DNA Excision RepairMethods in Molecular BiologyJune 19, 2020Amelia J. Hodges, Steven A. Roberts, John J. Wyrick(encodes T4 endoV with an N-terminal His-tag) into T7 Express lysγ chemically competent E. coli (NEB #C3010I) by mixing 10 ng of pET16-T4 endoV plasmid with 50 μL of E. coli suspension in a 15 mL culture tube

(17) Engineering selective competitors for the discrimination of highly conserved protein-protein interaction modulesNature CommunicationsDecember 1, 2019Charlotte Rimbault et al.labelled proteins, isotopically labelled constructs were expressed in BL21 pLysY (New England Biolabs, C3010I) from LB plates containing 30 µg mL−1 kanamycin. Cells pellets from overnight cultures in 10 mL of LB

(18) Distinctive Activation Mechanism for Angiotensin Receptor Revealed by a Synthetic NanobodyCellJanuary 24, 2019Wingler LM, McMahon C, Staus DP, Lefkowitz RJ, Kruse AC.and Virus Strains,Bacterial and Virus Strains T7 Express lysY BL21 E. coli,New England Biolabs,Cat# C3010I "Chemicals, Peptides, and Recombinant Proteins","Chemicals, Peptides, and Recombinant Proteins","Chemicals,

(19) Crystal structure of human nucleosome core particle containing enzymatically introduced CpG methylationFEBS Open BioJune 1, 2016Yoshifumi Fujii, Masatoshi Wakamori, Takashi Umehara, Shigeyuki Yokoyamaexpression of the M.SssI protein, T7 Express lysY Competent E. coli (High Efficiency) cells from NEB (cat. C3010I), harboring the pET15b‐M.SssI plasmid, were grown at 37 °C in LB medium containing 100 μg·mL−1 ampicillin

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