Pseudotyped ΔG-luciferase (G*ΔG-luciferase) rVSV from Kerafast

Product Specs
ItemPseudotyped ΔG-luciferase (G*ΔG-luciferase) rVSV
CompanyKerafast
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberEH1020-PM
TypeVirus
Quantity300uL

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Kerafast

2 Shaker Road, Unit B101

Shirley, MA 01464

United States

Phone: 1-800-546-1760
1-617-336-3228 (Mon–Fri 8:30AM–5:30PM EST)
Fax: 1-866-727-9826

Email: CustomerService@kerafast.com

Company Profile

Website: www.kerafast.com

(1) Deep immunological imprinting due to the ancestral spike in the current bivalent COVID-19 vaccineCell Reports MedicineNovember 21, 2023Qian Wang, Yicheng Guo, Anthony R. Tam, Riccardo Valdez, Aubree Gordon, Lihong Liu, David D. Hostrains,Bacterial and virus strains,Bacterial and virus strains ,, VSV-G pseudotyped ΔG-luciferase,Kerafast,Cat# EH1020-PM ,, Biological samples,Biological samples,Biological samples ,, “4-dose monovalent” sera,This paper,N/A “3-dose

(2) Evolving antibody evasion and receptor affinity of the Omicron BA.2.75 sublineage of SARS-CoV-2iScienceNovember 17, 2023Qian Wang, Zhiteng Li, Yicheng Guo, Ian A. Mellis, Sho Iketani, Michael Liu, Jian Yu, Riccardo Valdez, Adam S. Lauring, Zizhang Sheng, et al.strains,Bacterial and virus strains,Bacterial and virus strains ,, VSV-G pseudotyped ΔG-luciferase,Kerafast,Cat#EH1020-PM ,, Biological samples,Biological samples,Biological samples ,, 3 Shots WT sera,Wang et al.6,N/A 3

(3) Inactivation mechanisms of influenza A virus under pH conditions encountered in aerosol particles as revealed by whole-virus HDX-MSmSphereOctober 24, 2023Shannon C. David, Oscar Vadas, Irina Glas, Aline Schaub, Beiping Luo, Giovanni D'angelo, Jonathan Paz Montoya, Nir Bluvshtein, Walter Hugentobler, Liviana K. Klein, et al.Q33211). Recombinant Vesicular Stomatitis Virus stocks harboring a Firefly Luciferase gene instead of VSV G (Kerafast, EH1020-PM) were pseudotyped with either the VSV G protein (VSV-G) or the A/WSN/1933 HA and NA (VSV-H1N1). 8 ×

(4) Antibodies targeting a quaternary site on SARS-CoV-2 spike glycoprotein prevent viral receptor engagement by conformational lockingImmunityOctober 10, 2023Liu L, Casner RG, Guo Y, Wang Q, Iketani S, Chan JF, Yu J, Dadonaite B, Nair MS, Mohri H, et al.strains,Bacterial and virus strains,Bacterial and virus strains ,, VSV-G pseudotyped ΔG-luciferase,Kerafast,Cat#EH1020-PM hCoV-19/Hong_Kong/HKU-211129-001/2021,Shuai et al.64,N/A SARS-Related Coronavirus 2 Isolate USA-WA1/2020,BEI

(5) Changes in serum-neutralizing antibody potency and breadth post-SARS-CoV-2 mRNA vaccine boostiScienceApril 21, 2023Manoj S. Nair, Ruy M. Ribeiro, Maple Wang, Anthony D. Bowen, Lihong Liu, Yicheng Guo, Jennifer Y. Chang, Pengfei Wang, Zizhang Sheng, Magdalena E. Sobieszczyk, et al.CRL-11268 ,, Virus strains,Virus strains,Virus strains ,, VSV-G pseudotype Δ -luciferase,Kerafast,EH1020-PM WA1,BEI Resources,Cat# NR-52281 B.1.617.2 (Delta),BEI Resources,Cat# NR-55611 B.1.1.529.1 /BA.1.1

(6) Alarming antibody evasion properties of rising SARS-CoV-2 BQ and XBB subvariantsCellJanuary 19, 2023Qian Wang, Sho Iketani, Zhiteng Li, Liyuan Liu, Yicheng Guo, Yiming Huang, Anthony D. Bowen, Michael Liu, Maple Wang, Jian Yu, et al.strains,Bacterial and virus strains,Bacterial and virus strains ,, VSV-G pseudotyped ΔG-luciferase,Kerafast,Cat# EH1020-PM ,, Biological samples,Biological samples,Biological samples ,, Sera from 3 shots of mRNA-vaccinated

(7) Long‐term variations and potency of neutralizing antibodies against Omicron subvariants after CoronaVac‐inactivated booster: A 7‐month follow‐up studyJournal of Medical VirologyJanuary 1, 2023Xinjie Li, Yue Yin, Qianqian Cui, Weijin Huang, Qinghua Zou, Tao Shengene (Genbank: M27165) in pcDNA3.1, was used to amplify the VSV viral vector (G*ΔG‐VSV) (Kerafast: EH1020‐PM). 11 The pcDNA3.1.S2 recombinant plasmid (GenBank: MT613044) was constructed by inserting the S gene

(8) A second functional furin site in the SARS-CoV-2 spike proteinEmerging microbes & infectionsDecember 1, 2022Zhang Y, Zhang L, Wu J, Yu Y, Liu S, Li T, Li Q, Ding R, Wang H, Nie J, et al.293 T cells using lipofectamine 3000 (Invitrogen). Meanwhile, the G*ΔG-VSV (VSV G pseudotyped virus, EH1020-PM, Kerafast) was added to the cell culture supernatant. The cell culture medium was changed 6–8 h later.

(9) Characterization of the enhanced infectivity and antibody evasion of Omicron BA.2.75Cell Host & MicrobeNovember 9, 2022Yunlong Cao, Weiliang Song, Lei Wang, Pan Liu, Can Yue, Fanchong Jian, Yuanling Yu, Ayijiang Yisimayi, Peng Wang, Yao Wang, et al.RESOURCES,SOURCE,IDENTIFIER Bacterial,Bacterial,Bacterial ,, Trelief 5α Chemically Competent Cell,Tsingke,Cat# TSC-C01 G∗ΔG-VSV,Kerafast,Cat# EH1020-PM DH5α Chemically Competent Cell,Invitrogen,Cat# 12034013 ,, Cell lines,Cell lines,Cell lines ,, HEK293F

(10) Increased Resistance of SARS-CoV-2 Variant P.1 to Antibody NeutralizationbioRxivSeptember 15, 2022Pengfei Wang et al.Strains,Bacterial and Virus Strains,Bacterial and Virus Strains VSV-G pseudo-typed ΔG-luciferase,Kerafast,Cat# EH1020-PM ,, "Chemicals, Peptides, and Recombinant Proteins","Chemicals, Peptides, and Recombinant Proteins","Chemicals,

(11) Antigenic characterization of the SARS-CoV-2 Omicron subvariant BA.2.75Cell Host & MicrobeSeptember 6, 2022Qian Wang et al.strains,Bacterial and virus strains,Bacterial and virus strains ,, VSV-G pseudotyped ΔG-luciferase,Kerafast,Cat#EH1020-PM ,, Biological samples,Biological samples,Biological samples ,, Boosted sera,"Wang et al., 2022b",N/A BA.1

(12) Antibody evasion of SARS-CoV-2 Omicron BA.1, BA.1.1, BA.2, and BA.3 sub-lineagesCell Host & MicrobeAugust 10, 2022Jingwen Ai et al.strains,Bacterial and virus strains,Bacterial and virus strains ,, VSV-G pseudo-typed ΔG-luciferase,Kerafast,Cat# EH1020-PM ,, Biological samples,Biological samples,Biological samples ,, Convalescent human plasma samples,"Huashan

(13) Functional properties of the spike glycoprotein of the emerging SARS-CoV-2 variant B.1.1.529Cell reportsJune 14, 2022Wang Q, Anang S, Iketani S, Guo Y, Liu L, Katsamba PS, Shapiro L, Ho DD, Sodroski JG.strains,Bacterial and virus strains,Bacterial and virus strains ,, VSV-G pseudotyped ΔG-luciferase,Kerafast,Cat# EH1020-PM ,, Biological samples,Biological samples,Biological samples ,, CP1-CP11 sera,"(Wang et al., 2021a)",N/A V1-V6

(14) Comparison of BNT162b2-, mRNA-1273- and Ad26.COV2.S-Elicited IgG and Neutralizing Titers against SARS-CoV-2 and Its VariantsVaccines (Basel)May 27, 2022Nigam H. Padhiar et al.spike-expression plasmid and subsequently infected with rVSV-firefly-luciferase (Kerafast, catalog #: EH1020-PM) to yield VSV-pseudovirus containing a supernatant that was then collected, centrifuged at 500× g for

(15) The spike-ACE2 binding assay: An in vitro platform for evaluating vaccination efficacy and for screening SARS-CoV-2 inhibitors and neutralizing antibodiesJournal of Immunological MethodsApril 1, 2022Shuangzhe Zhang, Chunhui Gao, Tuhin Das, Shuhong Luo, Hao Tang, Xinyi Yao, Chih Yun Cho, Jingquiao Lv, Kino Maravillas, Valerie Jones, et al.antibodies (Nabs), small molecules, and serum samples G*ΔG-luciferase VSV (purchased from Kerafast: EH1020-PM) and VSV-SARS-CoV-2 pseudotyped viral particles were generated as previously described (Whitt, 2010;

(16) Antibodies to the SARS-CoV-2 receptor-binding domain that maximize breadth and resistance to viral escapebioRxivApril 1, 2022Tyler N. Starr et al.manufacturer’s instructions. One day post-transfection, cells were infected with VSV-luc (VSV-G) (Kerafast, EH1020-PM) for 1 h, rinsed three times with PBS, then incubated for an additional 24 h in complete media at 37°C.

(17) SARS-CoV-2 Infection Depends on Cellular Heparan Sulfate and ACE2SSRN Electronic JournalMarch 29, 2022Thomas Mandel Clausen et al.(USA-WA1/2020) BEI Resources Cat # NR-52281 VSV-G pseudotyped DG-luciferase or GFP VSV Kerafast Cat # EH1020-PM Bacterial and Virus Strains Chemicals, Peptides, and Recombinant Proteins SARS-CoV-2 (2019-nCoV) spike

(18) Structural and functional characterizations of infectivity and immune evasion of SARS-CoV-2 OmicronCellMarch 3, 2022Zhen Cui, Pan Liu, Nan Wang, Lei Wang, Kaiyue Fan, Qianhui Zhu, Kang Wang, Ruihong Chen, Rui Feng, Zijing Jia, et al.and virus strains ,, DH5α Chemically Competent Cell,Invitrogen,Cat#12034013 G∗ΔG-VSV,Kerafast,Cat#EH1020-PM ,, Cell lines,Cell lines,Cell lines ,, HEK293F cells,Thermo Fisher,Cat # 11625019 Huh7 cells,NIFDC,N/A ,, "Chemicals,

(19) SARS-CoV-2 Antibody Neutralization Assay Platforms Based on Epitopes Sources: Live Virus, Pseudovirus, and Recombinant S Glycoprotein RBDImmune NetworkNovember 23, 2021Endah Puji Septisetyani, Pekik Wiji Prasetyaningrum, Khairul Anam, Adi SantosorVSV lacking G glycoprotein gene in the genome can be obtained from Kerafast (rVSV-ΔG/G∗ luciferase, EH1020-PM). The rVSV is ready to use for pseudotyping by adding the virus solution into cells overexpressing S

(20) Functional differences among the spike glycoproteins of multiple emerging severe acute respiratory syndrome coronavirus 2 variants of concerniScienceNovember 19, 2021Wang Q, Nair MS, Anang S, Zhang S, Nguyen H, Huang Y, Liu L, Ho DD, Sodroski JG.strains,Bacterial and virus strains,Bacterial and virus strains ,, VSV-G pseudotyped ΔG-luciferase,Kerafast,Cat# EH1020-PM ,, Biological samples,Biological samples,Biological samples ,, CP1-CP6 sera,"(Wang et al., 2021a,

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Pseudotyped ΔG-luciferase (G*ΔG-luciferase) rVSV from Kerafast

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Pseudotyped ΔG-luciferase (G*ΔG-luciferase) rVSV from Kerafast

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