DRAQ5 ® from Cell Signaling Technology

Product Specs
ItemDRAQ5 ®
CompanyCell Signaling Technology
Price $263.00 Inquire
Catalog Number4084S
Quantity50 µl
Research AreaRelated Products
ApplicationsIF-IC, FC-FP

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

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(1) Anti-PECAM-1 antibodies: another tool for visualization of Reissner´s fiber and the subcommissural organ in rat central nervous systemBrain Struct FunctApril 4, 2026Lukáš Malčický, Ján Košuth, Daniel Barčák, Jarmila Zrubáková, Marie Vancová, Zuzana Daxnerová, Juraj Ševc, Anna Alexovič Matiašovácell nuclei, samples were counterstained with DRAQ5® (1:500, Cell Signaling Technology, Leiden, Netherlands, #4084) for 10 min, washed in PBS, transferred to glass slides and cover-slipped using ProLong Gold Antifade Reagent with DAPI

(2) IL-17 stimulates erythropoiesis in vivo by amplifying the response of erythroid progenitors to erythropoietinPLoS BiolDecember 11, 2025Qiu C. Wu, Aishwarya Swaminathan, Ashley Winward, Logan Lalonde, Yung Hwang, Noah Littman, Merav Socolovsky, Allon M. Kleinmeasured by flow-cytometric analysis of blood labeled with the Draq5 (Cell Signaling Technology, Item # 4084L) and with CD71 antibody (Biolegend, Clone RI7217). Erythroid precursors (ProE, EryA/B/C) were identified

(3) RIPK1 autophosphorylation at S161 mediates cell death and inflammationThe Journal of experimental medicineDecember 1, 2025Koerner L, Li X, Silnov E, Laurien L, Pasparakis M.treating three wells per genotype in each experiment with the cell-permeable fluorescent stain DRAQ5 (#4084L; Cell Signaling Technology, 500 nM) and imaging at a 2-h time point. Resulting images were analyzed

(4) Cellular Uptake and Tissue Retention of Microplastics in Black Soldier Fly LarvaeInsectsNovember 16, 2025Claudiu-Nicusor Ionica, Romelia Pop, Dragos Hodor, Irina Constantin, Ana Hiruta, Alexia-Teodora Hota, Alexandru Flaviu Tabaran, Sorana Daina, Andrei-Radu Szakacs, Adrian MacriOberkochen, Germany). Cellular nuclei were visualized with the fluorescent dye Draq5 (DRAQ5®, Cat. No. 4084, Cell Signaling Technology, Danvers, MA, USA), using an excitation wavelength of 543 nm and an emission

(5) Engineering Two-in-One Nanoparticles for Simultaneous Delivery of Graphene Quantum Dot and PemetrexedACS OmegaOctober 21, 2025Umut Can Öz, Berrin Küçüktürkmen, I. Jénnifer Gómez, Amr Elsherbeny, Seda Ipek Tekneci, Özgür Eşim, Selin Göksever, Umut Uğur Özköse, Sevgi Gülyüz, Claudia Bazán-Cobelo, et al.in λex = 405 nm and λem = 415–600 nm. The nuclear stain DRAQ5 (Cell Signaling Technology, Cat. No. 4084) imaging was carried out using λex = 633 nm and λem = 650–735 nm. Briefly, 2 × 104 MCF7 cells (ATCC,

(6) Microglia-specific regulation of lipid metabolism in Alzheimer's disease revealed by microglial depletion in 5xFAD MiceNat CommunOctober 15, 2025Ziying Xu, Sepideh Kiani Shabestari, Savannah Barannikov, Kevin F. Bieniek, Mathew Blurton-Jones, Juan Pablo Palavicini, Xianlin Hancatalog# MABN254), anti-GFAP (1:2000, MilliporeSigma, catalog# AB5541), DRAQ5 (1:1000, CST, catalog# 4084S), anti-NeuN (1:1000, MilliporeSigma, ABN90P), anti-Clec7a (1:100, InvivoGen, catalog# mabg-mdect), anti-Tmem119

(7) Depletion of a neural cell adhesion molecule disrupts both epithelial and germ layer identity in sea anemone embryosbioRxivAugust 4, 2025Anna Postnikova, Julissa Tello, Maristhela Alvarez, Grace Lee, Ilhan Ali, Christine M Field, Timothy J Mitchison, Katerina Ragkousi555 (Invitrogen, A21428). DNA was stained with either DAPI (1μg/ml) or Draq5 (1:2000, Cell Signaling 4084) and F-actin was stained with Alexa Fluor 488, 555 or 647 phalloidin (1:200, Invitrogen A12379, A34055

(8) A human-specific enhancer fine-tunes radial glia potency and corticogenesisNatureJuly 31, 2025Jing Liu, Federica Mosti, Hanzhi T. Zhao, Davoneshia Lollis, Jesus E. Sotelo-Fonseca, Carla F. Escobar-Tomlienovich, Camila M. Musso, Yiwei Mao, Abdull J. Massri, Hannah M. Doll, et al.dilution); anti-Synapsin1 (Synaptic Systems, 106008, 1:200 dilution); DRAQ5 (Cell Signaling Technology, 4084S, 1:1000 dilution); anti-Sox9 (Millipore, AB5535,1:2000 dilution); anti-Olig2(Millipore; MABN50,1:500

(9) ChromEMT: visualizing and reconstructing chromatin ultrastructure and 3D organization in situNature ProtocolsApril 1, 2025Horng D. Ou, Sebastien Phan, Thomas J. Deerinck, Akiko Inagaki, Mark H. Ellisman, Clodagh C. O’SheaCalcium chloride, dihydrate (Thermo-Fisher, cat. no. BP510-500) DRAQ5 (Cell Signaling, cat. no. 4084L) Saponin from quillaja bark (Sigma-Aldrich, cat. no. S4521-10G) Bovine serum albumin (Sigma-Aldrich,

(10) Pancreatic expression of CPT1A is essential for whole body glucose homeostasis by supporting glucose-stimulated insulin secretionJournal of Biological ChemistryJanuary 13, 2025Maggie P. Ducote, Caroline R. Cothern, Heidi M. Batdorf, Molly S. Fontenot, Thomas M. Martin, Maria Iftesum, Manas R. Gartia, Robert C. Noland, David H. Burk, Sujoy Ghosh, et al.were then washed twice for 5 min each in TBST, stained with DRAQ5 (Cell Signaling Technology; Cat # 4084L), diluted 1:500 in TBST + sodium azide (0.05%), and then kept at 4 °C until imaging. Cells were imaged

(11) Recruitment of FBXO22 for targeted degradation of NSD2Nature chemical biologyDecember 1, 2024Nie DY, Tabor JR, Li J, Kutera M, St-Germain J, Hanley RP, Wolf E, Paulakonis E, Kenney TMG, Duan S, et al.Donkey anti-Mouse (LI-COR 926–32212; RRID:AB_621847) diluted 1:1,000 and DRAQ5 stain (Cell Signaling 4084) diluted 1:2,000 in 5% BSA for 1 h at room temperature with agitation. The cells were washed 3× by PBS

(12) Estrogen receptor alpha mediated repression of PRICKLE1 destabilizes REST and promotes uterine fibroid pathogenesisbioRxivSeptember 20, 2024Michelle M. McWilliams, Faezeh Koohestani, Wendy N. Jefferson, Sumedha Gunewardena, Kavya Shivashankar, Riley A. Wertenberger, Carmen J. Williams, T. Rajendra Rajendra Kumar, Vargheese M. ChennathukuzhiSuperSignal West Pico Chemiluminescent Substrate (34080) were purchased from Thermo Scientific (Rockford, IL). DRAQ5 (4084L), EZH2 (D2C9) XP Rabbit mAb, and GAPDH (D16H11) pAb (5174) were purchased from Cell Signaling (Danvers,

(13) TRIM44, a Novel Prognostic Marker, Supports the Survival of Proteasome-Resistant Multiple Myeloma CellsCellsAugust 26, 2024Vu T, Wang Y, Fowler A, Simieou A, McCarty N.room temperature, washed three times with PBS, and incubated with DRAQ5 (Cell Signaling Technology, 4084) for 30 min. Slides were analyzed by confocal microscopy (Leica TCS SP5, Leica Microsystems, Exton,

(14) TRIM44 enhances autophagy via SQSTM1 oligomerization in response to oxidative stressScientific reportsAugust 16, 2024Wang Y, Lyu L, Vu T, McCarty N.h at room temperature, washed 3 times with PBS and incubated with DRAQ5 (Cell Signaling Technology, 4084) for 30 min. Slides were analyzed by confocal microscope Leica TCS SP5 and processed with the software

(15) Retinoic acid generates a beneficial microenvironment for liver progenitor cell activation in acute liver failureHepatology CommunicationsAugust 1, 2024Sai Wang, Frederik Link, Stefan Munker, Wenjing Wang, Rilu Feng, Roman Liebe, Yujia Li, Ye Yao, Hui Liu, Chen Shao, et al.#A-21422, Invitrogen); Alexa Flour 488 goat anti-rabbit IgG (cat. #A-11008, Invitrogen); DRAQ5 (cat. #4084L, Cell Signaling Technology); GAPDH antibody (cat. #32233, Santa Cruz Biotechnology); Histone H1 antibody

(16) Distributed X chromosome inactivation in brain circuitry is associated with X-linked disease penetrance of behaviorCell ReportsApril 23, 2024Eric R. Szelenyi, Danielle Fisenne, Joseph E. Knox, Julie A. Harris, James A. Gornet, Ramesh Palaniswamy, Yongsoo Kim, Kannan Umadevi Venkataraju, Pavel Ostensamples were co-stained with 1 μM DAPI (to mark damaged cells) and DRAQ5 (Cell Signaling Technology; 4084) (to label viable cells) for at least 10 min prior to sorting. Cell analysis and sorting was performed

(17) Microsomal triglyceride transfer protein is necessary to maintain lipid homeostasis and retinal functionThe FASEB JournalMarch 15, 2024Catharina R. Grubaugh, Anuradha Dhingra, Binu Prakash, Diego Montenegro, Janet R. Sparrow, Lauren L. Daniele, Christine A. Curcio, Brent A. Bell, M. Mahmood Hussain, Kathleen Boesze‐Battagliatemperature in the dark, followed by PBS washes (23). The sections were counterstained with 5 μM DRAQ5 (4084, Cell Signaling Technology), washed in PBS, mounted in Prolong Gold Antifade and imaged. For LipidTOX™

(18) TRIM44 promotes autophagy through SQSTM1 oligomerization in the response to oxidative stress induced by Arsenic Trioxide in cancer cellsResearch SquareFebruary 21, 2024Nami McCarty, Yuqin Wang, Lin Lyu, Trung Vuh at room temperature, washed 3 times with PBS and incubated with DRAQ5 (Cell Signaling Technology, 4084) for 30 min. Slides were analyzed by Confocal microscopy (Leica TCS SP5). To quantify indicated protein

(19) The Interplay of TGF-β1 and Cholesterol Orchestrating Hepatocyte Cell Fate, EMT, and Signals for HSC ActivationCellular and Molecular Gastroenterology and HepatologyJanuary 1, 2024Sai Wang, Frederik Link, Mei Han, Roohi Chaudhary, Anastasia Asimakopoulos, Roman Liebe, Ye Yao, Seddik Hammad, Anne Dropmann, Marinela Krizanac, et al.Donkey Anti-Rabbit IgG (H+L) (cat. 711-546-152; Jackson ImmunoResearch, West Grove, PA); DRAQ5 (cat. 4084L; Cell Signaling Technology); glyceraldehyde-3-phosphate dehydrogenase antibody (cat. 32233; Santa Cruz

(20) Histological and functional characterization of 3D human skin models mimicking the inflammatory skin diseases psoriasis and atopic dermatitisDisease Models & MechanismsJanuary 1, 2024Jasmin Scheurer, Birgit Sauer, Jule Focken, Martina Giampetraglia, Annika Jäger, Christian M. Schürch, Bettina Weigelin, Birgit Schittekfollowed by detection of cells positive for nuclear staining with DRAQ5 (Cell Signaling Technology, 4084L). Subsequently measurements for mean nuclear expressions of respective markers were sorted in Excel

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DRAQ5 ®

DRAQ5 ® from Cell Signaling Technology

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DRAQ5 ® from Cell Signaling Technology

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