Bsu36I from New England Biolabs

Product Specs
ItemBsu36I
CompanyNew England Biolabs
Price $72.00 Inquire
Catalog NumberR0524S
Quantity1000 units
Concentration10 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

CC/TNAGG

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Workflow for Crystallographic Fragment Screening by Crystal Soaking for Protein Targets: A Case Study on Thioredoxin Glutathione Reductase from Schistosoma mansoniBIO-PROTOCOLApril 20, 2026Lauro R. S. Neto, Bogar O. Montoya, José Brandão-Neto, Kelly Parker, Frank Von Delft, Nicholas Furnham, Raymond J. Owens, Floriano P. Silva-Jrkit (Molecular Dimensions, catalog number: MD1-104) 6. Bsu36I (New England Biolabs, catalog number: R0524L) 7. CaCl2·2H2O (Sigma-Aldrich, catalog number: 223506) 8. Chloramphenicol (Sigma-Aldrich, catalog number:

(2) Scaling perturbations: beyond genome-scale CRISPR screensbioRxivJanuary 21, 2026Anran Tang, Rico C. Ardy, Rafaela E. Mendes, Thomas M. NormanNEBuilder® HiFi DNA Assembly Master Mix (NEB, E2621). To remove any undigested backbone, Bsu36I (NEB, R0524S) restriction enzyme was added, incubated at 37 °C for 15 minutes and heat inactivated at 80 °C for 20

(3) In vivo screen reveals specific roles of Hippo pathway components in development and regenerationLife Sci AllianceDecember 29, 2025Susanna E Riley, Michaela Noskova Fairley, Shijia Xia, Richard Cunningham, Justyna Cholewa-Waclaw, Yi Feng, Carsten Gram Hansen(25G x 1”—Nr.18),300400; BD BstXI with NEB buffer 3.1,R0113S; NEB Bsu36I with NEB buffer 1.1 (10X),R0524S; NEB Calcium nitrate tetrahydrate (Ca(NO3)2·4H2O),C1396; Sigma-Aldrich "Capillary glass (1.2 mm outer

(4) A programmable, selection-free CRISPR interference system in Staphylococcus aureus for long-term host interaction studiesiScienceSeptember 19, 2025Roni Miah, Mona Johannessen, Morten Kjos, Christian S. Lentz,Thermo Scientific™,Cat NO: 89833 DNase I,Sigma-Aldrich,Cat NO: AMPD1 Bsu36I,New England Biolabs,Cat NO: R0524S T4 DNA Ligase,New England Biolabs,Cat NO: M0202S 1% penicillin/streptomycin,Gibco™,Cat NO: 11548876 Phorbol 12-myristate 13-acetate

(5) Deep mutational scanning reveals EGFR mutations conferring resistance to the 4th-generation EGFR tyrosine kinase inhibitor BLU-945NPJ Precis OncolAugust 20, 2025Yueyang Wang, Yuan Hao, Michela Ranieri, Tigran M. Abramyan, Lev Tsidilkovski, Michelle Hollenberg, Alfonso Lopez, Xavier T. R. Moore, Fiona Sherman, Jiehui Deng, et al.library construction, the plasmid was linearized using the restriction enzyme BSU36I (New England Biolabs #R0524S), which recognizes distinct sequences flanking the region of interest. The digestion reaction was prepared

(6) Protocol for reconstituting enzymatic activities for ultra-large histone methyltransferases NSD1 and SETD2 using a baculovirus expression systemSTAR ProtocJuly 18, 2025Chen-I Hsu, Emily Yeh, Carol C.L. Chen, Mahnue Sahn, Jia-Ray Yustudy,N/A BAC10:KO1629,Zhang et al.2,N/A ,, Other,Other,Other ,, Bsu36i,New England Biolabs,Cat# R0524S ESF 921 Protein-free insect cell culture medium,Expression Systems,Cat#96-001-01 500 mL Erlenmeyer

(7) RNA degradation triggered by decapping is largely independent of initial deadenylationThe EMBO JournalSeptember 25, 2024Léna Audebert, Frank Feuerbach, Mostafa Zedan, Alexandra P Schürch, Laurence Decourty, Abdelkader Namane, Emmanuelle Permal, Karsten Weis, Gwenaël Badis, Cosmin Saveanu(IAA),Sigma-Aldrich,I2886 Beta-estradiol,Sigma-Aldrich,E2758 Doxycycline,Sigma-Aldrich,D3447 4-Thiouracil,Sigma-Aldrich,440736 BamHI-HF,New England Biolabs,R3136 Bsu36I,New England Biolabs,R0524 NotI-HF,New England Biolabs,R3189 SstII,ThermoFisher,45230-018 SmaI,New England Biolabs,R0141 XhoI,New England Biolabs

(8) Deciphering the functional impact of Alzheimer’s Disease-associated variants in resting and proinflammatory immune cellsmedRxivSeptember 20, 2024Marielle L. Bond, Ivana Y. Quiroga-Barber, Susan D’Costa, Yijia Wu, Jessica L. Bell, Jessica C. McAfee, Nicole E. Kramer, Sool Lee, Mary Patrucco, Douglas H. Phanstiel, et al.disrupt the downstream cloning of the MPRA library. The plasmid was first digested with Bsu36I (NEB, #R0524S), KpnI-HF (NEB, #R3142S), and rSAP. Remove-KpnI-oligo (Supplementary Table 1) was ligated into the digested

(9) Bacterial genome engineering using CRISPR-associated transposasesNature ProtocolsMarch 1, 2024Diego Rivera Gelsinger, Phuc Leo H. Vo, Sanne E. Klompe, Carlotta Ronda, Harris H. Wang, Samuel H. SternbergR3140S/L/T/M)XhoI restriction enzyme (NEB, cat. no. R0146S/M)Bsu36I restriction enzyme (NEB, cat. no. R0524S/L)SalI restriction enzyme (NEB, cat. no. R0138S/T/L/M)CutSmart or rCutSmart buffer, 5× (included with

(10) Solid-to-liquid phase transition in the dissolution of cytosolic misfolded-protein aggregatesiScienceDecember 15, 2023Alexis Tomaszewski, Rebecca Wang, Eduardo Sandoval, Jin Zhu, Jian Liu, Rong Liworkstation (Eppendorf) and 150 μL of refreshed logarithmic phase cultures plus 0.2 μg of Bsu36I (NEB, Cat #: R0524S) linearized FlucSM-GFP-URA3 DNA were added to the Frozen-EZ Yeast solution mix. The transformation mixtures

(11) Binding of the nuclear ribonucleoprotein family member FUS to RNA prevents R-loop RNA:DNA hybrid structuresThe Journal of biological chemistryOctober 1, 2023Thompson VF, Wieland DR, Mendoza-Leon V, Janis HI, Lay MA, Harrell LM, Schwartz JC.and the gene inserted for RNA transcription was Flag-FUS (13). Bsu36I restriction enzyme (50 U, NEB, R0524S) was used to linearize plasmid (10 μg) by incubating at 37 °C for a minimum of 2 h in 1× CutSmart buffer

(12) Screening of Membrane Protein Production by Comparison of Transient Expression in Insect and Mammalian CellsBiomoleculesMay 11, 2023Jagan Mohan Kaipa, Ganna Krasnoselska, Raymond J. Owens, Joop van den Heuveltransfer plasmid (500/750 ng) was added to Bsu36I (New England Biolabs, Frankfurt-am-Main, Germany, #R0524S), linearized empty DsRed bacmid (500 ng) (the bacmid was a kind gift from Prof. Ian Jones) diluted in

(13) Bacterial genome engineering using CRISPR RNA-guided transposasesbioRxivMarch 25, 2023Diego R. Gelsinger, Phuc Leo H. Vo, Sanne E. Klompe, Carlotta Ronda, Harris Wang, Samuel H. SternbergR3140S/L/T/M)XhoI restriction enzyme (NEB cat. no. R0146S/M)Bsu36I restriction enzyme (NEB cat. no. R0524S/L)SalI restriction enzyme (NEB cat. no. R0138S/T/L/M)CutSmart or rCutSmart buffer, 5X (included with

(14) Thermostable negative-marker foot-and-mouth disease virus serotype O induces protective immunity in guinea pigsApplied microbiology and biotechnologyFebruary 1, 2023Ranjitha HB, Dhanesh VV, Hosamani M, Sreenivasa BP, Jabeen U, Biswal JK, Saravanan P, Sanyal A, Bhanuprakash V, Basagoudanavar SH.was digested with Nhe I (New England Biolabs, USA, #R0131S) and Bsu36 I (New England Biolabs, USA, #R0524S) and used to replace the corresponding fragment in pT7-OR2/75. The new recombinant plasmid was designated

(15) Human ALS/FTD brain organoid slice cultures display distinct early astrocyte and targetable neuronal pathologyNature neuroscienceNovember 1, 2021Szebényi K, Wenger LMD, Sun Y, Dunn AWE, Limegrover CA, Gibbons GM, Conci E, Paulsen O, Mierau SB, Balmus G, et al.Blood and Tissue kit (69506). DNA (5 µg) was restriction digested with Bsu36I (New England Biolabs, R0524S) and ran on 0.8% agarose gels accompanied by molecular weight markers II (Roche, 11218590910) and III

(16) ADAR2 mislocalization and widespread RNA editing aberrations in C9orf72-mediated ALS/FTDActa neuropathologicaJuly 1, 2019Moore S, Alsop E, Lorenzini I, Starr A, Rabichow BE, Mendez E, Levy JL, Burciu C, Reiman R, Chew J, et al.NEBuilder HiFi DNA Assembly Cloning Kit (NEB-E2621S), with ADAR2-pEZ-Lv111 destination vector linearized with XbaI and Bsu36I (NEB-R0524S) and gel purified. Fragments were amplified via PCR (Thermo Scientific-F549S) using ADAR2-pEZ-Lv111

(17) Precision Tagging: A Novel Seamless Protein Tagging by Combinational Use of Type II and Type IIS Restriction EndonucleasesBIO-PROTOCOLFebruary 5, 2018Zhen Xu, Yan-Ning Rui, John P. Hagan, Dong H. KimR3136S)SalI-HF (New England Biolabs, catalog number: R3138S)Bsu36I (New England Biolabs, catalog number: R0524S) Type IIS restriction endonucleases such as: BsaI-HF (New England Biolabs, catalog number: R3535S)BbsI-HF

(18) Gene therapy for human ovarian cancer cells using efficient expression of Fas gene combined with γδT cellsMolecular medicine reportsOctober 1, 2017Lin J, Zeng D, He H, Tan G, Lan Y, Jiang F, Sheng S.enzyme digestion using Nhe I (catalog no. R0131S; New England BioLabs, Inc.) and Bsu36I (catalog no. R0524S; New England BioLabs, Inc.). For enzyme digestion, PCR products were digested with XbaI and Kpn I (10

(19) Cell line with endogenous EGFRvIII expression is a suitable model for research and drug development purposesOncotargetMay 31, 2016Stec WJ, Rosiak K, Siejka P, Peciak J, Popeda M, Banaszczyk M, Pawlowska R, Treda C, Hulas-Bigoszewska K, Piaskowski S, et al.Cat. no. OHS5922) previously digested with Bsu36I/SnaBI enzymes (both New England Biolabs, Cat. no. R0524 and R0130, respectively). Electrophoretic analysis and DNA sequencing were performed to verify the resulting

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Bsu36I

Bsu36I from New England Biolabs

Sequence

New England Biolabs

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Bsu36I from New England Biolabs

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