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BstUI
BstUI
Supplier Images
BstUI
BstUI from New England Biolabs
Be the first to write a review!
Citations:
(15)
Supplier Page
Supplier Page from
New England Biolabs for
BstUI
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Product Specs
Company Info
Citations
Reviews
Product Specs
Item
BstUI
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0518S
Concentration
10 u/µL
Quantity
1000 units
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
CG/CG
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (15)
(1)
DNA methyltransferases are complementary in maintaining DNA methylation in embryonic stem cells
iScience
September 16, 2022
Liu Y, Xu Z, Shi J, Zhang Y, Yang S, Chen Q, Song C, Geng S, Li Q, Li J,
et al
.
MF02801S
ClaI
,"Monad, China",Cat. # MF00501M
HhaI
,"
NEB
, USA",Cat. #R0139V
BstUI
,"
NEB
, USA",Cat. #
R0518L
,, Critical commercial
assays
,Critical commercial
assays
,Critical commercial
assays
,, AxyPrep DNA
(2)
Association of polymorphism
TP53
Arg72Pro with radon-induced lung cancer in the Kazakh population
Vavilov Journal of Genetics and Breeding
August 24, 2019
O. Bulgakova, A. Kussainova, A. Kakabayev, А. Kausbekova, R. Bersimbaev
bp enzyme Restricted product length and corresponding genotype
BstUI
Arg72Arg – 199 bp; (cat no.
R0518S
; Arg72Pro – 199 bp,
NEB
, USA) 113 bp, 87 bp; Pro72Pro –113bp, 87 bp consumption using the method proposed
(3)
Commensal Neisseria kill Neisseria gonorrhoeae through a DNA-dependent mechanism in mice
Cell Host & Microbe
August 1, 2019
Won Jong Kim, Dustin Higashi, Maira Goytia, Maria A. Rendón, Michelle Pilligua-Lucas, Matthew Bronnimann, Jeanine A. McLean, Joseph Duncan, David Trees, Ann E. Jerse,
et al.
R0630S
BamHI
,
NEB
,Cat#
R0136S
NotI
,
NEB
,Cat# R1089S
SphI
,
NEB
,Cat# R1082S
PstI
,
NEB
,Cat#
R0140S
BstUI
,
NEB
,Cat#
R0518S
HaeII
,
NEB
,Cat#
R0107S
SacI
,
NEB
,Cat#
R0156S
NdeI
,
NEB
,Cat#
R0111S
ClaI
,
NEB
,Cat#
R0197S
SalI
,
NEB
,Cat#
(4)
Long-Term In Vitro Expansion of Epithelial Stem Cells Enabled by Pharmacological Inhibition of PAK1-ROCK-Myosin II and TGF-β Signaling
Cell reports
October 16, 2018
Zhang C, Lee HJ, Shrivastava A, Wang R, McQuiston TJ, Challberg SS, Pollok BA, Wang T.
(5)
A study on genetic polymorphism in Matrix Metalloproteinases-3 in oral submucous fibrosis patients and in healthy individuals
Applied Cancer Research
December 1, 2017
Preethi A. Poonja, Atul P. Sattur, Krishna N. Burde, Shivaprakash V. Hiremath
2).
Restriction enzyme digestion
All
PCR
products (10 μl) were digested with 10units of
BstUI
(
NEB
#
R0518
)/
Tth111I
(
NEB
# R0185) restriction endonuclease enzyme.
Restriction enzyme digestion
was carried out
(6)
DNA sequence homology induces cytosine-to-thymine mutation by a heterochromatin-related pathway in Neurospora
Nature Genetics
June 1, 2017
Eugene Gladyshev, Nancy Kleckner
using 0.2 μg of purified genomic DNA from the previous step and 4 μl of
BstUI
(10 U/μl;
NEB
, cat. no.
R0518S
). Reactions were incubated for 3 hours at 50°C.
BstUI
was inactivated by
Proteinase K
(by adding 2 μl
(7)
The CpG Dinucleotide Adjacent to a κB Site Affects NF-κB Function through Its Methylation
International Journal of Molecular Sciences
March 1, 2017
Tao Wang et al.
DNA methylation
was verified by digestion with the methylation-sensitive restriction endonuclease
BstUI
(
R0518
,
NEB
). HEK
293T cells
were
transfected
with the indicated plasmids using the X-tremegene HP (
Roche
).
(8)
Vascular endothelial growth factor (VEGF) gene polymorphism in oral submucous fibrosis subjects - A preliminary study
Asian Journal of Medical Sciences
August 31, 2016
Devishree V Rai, Kruthika S Guttal, Bhusan B Kulkarni, Shivaprakash Hiremath, Krishna N Burde
incubating at 37°C for 15 minutes All
PCR
products (10μl) were digested with 10units of
BstUI
(
NEB
#
R0518
)/
Tth111I
(
NEB
# R0185) restriction endonuclease enzyme. The
restriction enzyme digestion
was subjected
(9)
Direct evidence for sequence-dependent attraction between double-stranded DNA controlled by methylation
Nature Communications
April 1, 2016
Jejoong Yoo, Hajin Kim, Aleksei Aksimentiev, Taekjip Ha
was estimated by digesting the dsDNA products with the
BstUI
restriction enzyme (
New England BioLabs
,
R0518L
), which can cut only unmethylated CGCG sequence, and subsequent
electrophoresis
of the digested fragments
(10)
BRAF mutation-specific promoter methylation of FOX genes in colorectal cancer
Clinical Epigenetics
December 1, 2013
Eddy H van Roon, Arnoud Boot, Ashwin A Dihal, Robert F Ernst, Tom van Wezel, Hans Morreau, Judith M Boer
and sequentially digested with two methylation-sensitive restriction enzymes (
HpaII
#
R0171
and
BstUI
#
R0518
,
New England Biolabs
). Digested linker-ligated DNA was used as a template for
PCR amplification
(20
(11)
Desmoglein 1 deficiency results in severe dermatitis, multiple allergies and metabolic wasting
Nature Genetics
October 1, 2013
Liat Samuelov et al.
(12)
Epigenetic regulation of
Sox4
during palate development
Epigenomics
August 20, 2013
Ratnam S Seelan, Partha Mukhopadhyay, Dennis R Warner, Cynthia L Webb, Michele Pisano, Robert M Greene
unmethylated and methylated plasmids with
AclI
(Cat. No.
R0598S
;
New England BioLabs
) and
BstUI
(Cat. No.
R0518S
;
New England BioLabs
). The plasmids were purified using Qiagen's
PCR kit
and the DNA used for
transfection
(13)
Epigenetic Regulation of
TNFA
Expression in Periodontal Disease
Journal of Periodontology
February 2, 2013
Shaoping Zhang, Silvana P. Barros, Antonio J. Moretti, Ning Yu, Jing Zhou, John S. Preisser, Mihai D. Niculescu, Steven Offenbacher
without the supplement of SAM. The completion of the
in vitro
methylation was confirmed by
BstUI
(Cat.#
R0518S
,
New England Biolabs
) digestion. After
purification
, modified insert was directly religated with the
(14)
Epigenetic impact of simulated maternal grooming on estrogen receptor alpha within the developing amygdala
Brain, Behavior, and Immunity
October 1, 2011
Michelle N. Edelmann, Anthony P. Auger
HhaI
. In each case, control DNA received identical treatment, except without the enzyme.
BSTUI
(Cat. #
R0518S
,
New England BioLabs
, Ipswich, MA) recognizes CGCG sites and cleaves DNA between the G and the C if
(15)
In Situ
Detection of Benzimidazole Resistance in Field Isolates of
Venturia inaequalis
in Indiana
Plant Disease
June 1, 2010
Kacie L. Quello, Kimberly S. Chapman, Janna L. Beckerman
BtubF14 and BtubR was digested with 1 unit of
BstUI
restriction enzyme (
New England BioLabs
, cat. no.
R0518S
) or 1 unit of
Cac8I
restriction enzyme (
New England BioLabs
cat. no.
R0579S
) in 1× buffer 4, provided
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BstUI from New England Biolabs
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