BssHII from New England Biolabs

Product Specs
ItemBssHII
CompanyNew England Biolabs
Price $75.00 Inquire
Catalog NumberR0199S
Quantity500 units
Concentration4-20 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

G/CGCGC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) OT-Curtains: an approach for studying protein interactions with DNA ends using optical tweezers and confocal fluorescence microscopyNucleic Acids ResApril 28, 2026Sara De Bragança, Clara Aicart-Ramos, Ángel Rivera-Calzada, Raquel Arribas-Bosacoma, Mark S Dillingham, Oscar Llorca, Fernando Moreno-Herrerosection) at a final concentration of 2.7 nM is digested with 1.25 units of BssHII (New England Biolabs, #R0199S) to linearize the DNA in 6.25 μl buffer provided by the manufacturer. Simultaneously, the plasmid is

(2) CBP-IDRs regulate acetylation and gene expressionCell RepMarch 24, 2026Katie L. Gelder, Nicola A. Carruthers, Grace Gilbert, Laura J. Harrison, Brychan V. Evans, Thomas I. Evans, Sophie S. Ball, Mark Dunning, Timothy D. Craggs, Alison E. Twelvetrees, et al.(PNK),NEB,EK0031 NruI-HF,NEB,R3192S PmlI,NEB,R0532S Quick Calf Intestinal Phosphatase (CIP),NEB,M0525S BamHI-HF,NEB,R3136S MluI-HF,NEB,R3198S BssHII,NEB,R0199S BbsI-HF,NEB,R3539S QuickExtract solution,Lucigen,QE0905T T7 Endonuclease 1 (T7E1) enzyme,NEB,M0689S TRIzol,ThermoFisher,15596018 Turbo

(3) MASTR-seq enables multiplexed analysis of short tandem repeats with sequencingCell Rep MethodsMarch 23, 2026Chuanbin Su, Han-Seul Ryu, Keerthivasan Raanin Chandradoss, Thomas Malachowski, Ravi Boya, Linda Zhou, Hoa Emma Nguyen, Esteban O. Mazzoni, Kristen J. Brennand, Jennifer E. Phillips-Cremins,New England Biolabs,Cat# E6056S EcoRI,New England Biolabs,Cat# R0101S BssH II,New England Biolabs,Cat# R0199S AP Detection buffer 10X (Alkaline Phos. buffer),Gene Link,Cat# 40-5031-10 AP Substrate CDP Star Tropix,Gene

(4) Developing drug-like single-domain antibodies (VHH) from in vitro librariesmAbsDecember 1, 2025Erasmus MF, Teixeira AAR, Molina E, Rodriguez Carnero LA, Li J, Knight D, Di Niro R, Leal-Lopes C, Fanni A, Troell H, et al.gradient centrifugation. The product was linearized using BssHII and NheI restriction enzymes (NEB, #R0199 and #R3131) and gel purified. Phage display sublibrary assembly and transformation CDR1 and CDR2 from

(5) Rapid and quantitative functional interrogation of human enhancer variant activity in live miceNat CommunJanuary 6, 2025Ethan W. Hollingsworth, Taryn A. Liu, Joshua A. Alcantara, Cindy X. Chen, Sandra H. Jacinto, Evgeny Z. Kvonthe H11 homology arms via PCR. A dual-enSERT-2 plasmid (2 μg) was digested overnight with PauI (NEB, R0199) at 50 °C in rCutSmart Buffer. The following day, the reaction was inactivated by incubation at 65 °C

(6) Virus-induced vesicular acidification enhances HIV immune evasionbioRxivDecember 21, 2024Marianne E. Yaple-Maresh, Giselle G. Flores, Gretchen E. Zimmerman, Francisco Gomez-Rivera, Kathleen L. Collins,New England BioLabs,Cat. R0145 MluI Enzyme,New England BioLabs,Cat. R3198 BssHII Enzyme,New England BioLabs,Cat. R0199S SalI Enzyne,New England BioLabs,Cat. R0138S NheI-HF Enzyme,New England BioLabs,Cat. R3131S T4 DNA Ligase

(7) A next-generation Fab library platform directly yielding drug-like antibodies with high affinity, diversity, and developabilitymAbsJanuary 1, 2024Ferrara F, Fanni A, Teixeira AAR, Molina E, Leal-Lopes C, DeAguero A, D'Angelo S, Erasmus MF, Spector L, Rodriguez Carnero LA, et al.into a VL-CL – VH-CH1 PCR product. The Fab amplicons were digested using BssHII and NheI (R0131L and R0199L, NEB) and ligated into the pANDA5 Fab phagemid vector, derived from the pDAN5 scFv vector15 previously

(8) Rapid and Quantitative Functional Interrogation of Human Enhancer Variant Activity in Live MicebioRxivDecember 10, 2023Ethan W. Hollingsworth, Taryn A. Liu, Sandra H. Jacinto, Cindy X. Chen, Joshua A. Alcantara, Evgeny Z. Kvonvia PCR. For dual-enSERT-2.2, a dual-enSERT-2.1 plasmid (1 μg) was digested overnight with PauI (NEB, R0199) at 50°C in rCutSmart Buffer. The following day, the reaction was inactivated by incubation at 65°C

(9) Cell type specializations of the vocal-motor cortex in songbirdsCell ReportsNovember 28, 2023Alexander A. Nevue, Benjamin M. Zemel, Samantha R. Friedrich, Henrique von Gersdorff, Claudio V. MelloDIG RNA Labeling Mix,Roche,Cat#11277073910 Triethanolamine,Fisher,Cat#T350 Formamide,Fisher,Cat#F84 BCIP/NBT,Perkin-Elmer,Cat#NEL937 Acetic Anhydride,Sigma,Cat#A6404 Triton X-100,Sigma,Cat#T8787 BSSHII,NEB,Cat#R0199S T3 RNA Polymerase,Promega,Cat#P208C Tyramide conjugates,Invitrogen,Cat#B40952; B40953 CUBIC reagent,Lee

(10) Identification of an autotransporter peptidase of Rickettsia rickettsii responsible for maturation of surface exposed autotransportersPLOS PathogensJuly 31, 2023Adam M. Nock, Karin Aistleitner, Tina R. Clark, Dan Sturdevant, Stacy Ricklefs, Kimmo Virtaneva, Yixiang Zhang, Naila Gulzar, Neelam Redekar, Amitiva Roy, et al.GeneJet PCR Purification Kit (Thermoscientific, K0702), digested with BsiWi-HF (NEB, R3553S) and BssHII (NEB, R0199S), then re-purified using the GeneJet PCR Purification Kit per manufacturer’s instructions. Plasmids

(11) Simultaneous affinity maturation and developability enhancement using natural liability-free CDRsmAbsDecember 31, 2022Andre A. R. Teixeira et al.with 4 μg of the yeast display vector pSYD previously digested with the enzymes BssHII and NheI (NEB #R0199S and #R0131S) by electroporation using the method described previously.71 Phase 2 library combo construction For

(12) Multiplexed promoter and gene editing in wheat using a virusâ€based guide RNA delivery systemPlant Biotechnology JournalSeptember 7, 2022Wei Wang, Zitong Yu, Fei He, Guihua Bai, Harold N. Trick, Alina Akhunova, Eduard Akhunovexpressing BSMV γ chain carrying sgRNAs were linearized with BssHII (New England BioLabs, catalogue number R0199S). In vitro transcription was performed in 20 μL reaction volume using HiScribe™ T7 High Yield RNA Synthesis Kit

(13) Emergence of sex-specific transcriptomes in a sexually dimorphic brain nucleusCell ReportsAugust 2, 2022Samantha R. Friedrich, Alexander A. Nevue, Abraão L.P. Andrade, Tarciso A.F. Velho, Claudio V. MelloDIG RNA Labeling Mix,Roche,Cat#11277073910 Triethanolamine,Fisher,Cat#T350 Formamide,Fisher,Cat#F84 BCIP/NBT,Perkin-Elmer,Cat#NEL937 Acetic Anhydride,Sigma,Cat#A6404 Triton X-100,Sigma,Cat#T8787 BSSHII,NEB,Cat#R0199S T3 RNA Polymerase,Promega,Cat#P208C ,, Critical commercial assays,, ,, RNeasy Micro Kit,Qiagen,Cat#74004 RNA

(14) Intrinsic antiviral immunity of barrier cells revealed by an iPSC-derived blood-brain barrier cellular modelCell reportsMay 31, 2022Cheng Y, Medina A, Yao Z, Basu M, Natekar JP, Lang J, Sanchez E, Nkembo MB, Xu C, Qian X, et al.Sp6 RNA polymerase,NEB,Cat#M0207S SacI-HF,NEB,Cat#R3156S ClaI,NEB,Cat#R0197S AatII,NEB,Cat#R0117S BsHII,NEB,Cat#R0199S Human brain microvascular endothelial cell lysate,ScienCell,Cat#1006 Sodium Citrate Dihydrate,Fisher

(15) Healthy humans can be a source of antibodies countering COVID-19BioengineeredMay 1, 2022Velappan N, Nguyen HB, Micheva-Viteva S, Bedinger D, Ye C, Mangadu B, Watts AJ, Meagher R, Bradfute S, Hu B, et al.all 18 clones were excised from PDNL6 plasmids by restriction digestion with enzymes BssHII (NEB, #R0199S) and NheI (NEB, #R3131S) and gel purified. These genes were inserted into a yeast scFv-Fc expression

(16) Cleavage of viral DNA by restriction endonucleases stimulates the type II CRISPR-Cas immune responseMolecular CellMarch 1, 2022Pascal Maguin, Andrew Varble, Joshua W. Modell, Luciano A. MarraffiniEnzyme,New England Biolabs,Catalog No. R0102 BssHII Restriction Enzyme,New England Biolabs,Catalog No. R0199 DNase I,Sigma,Catalog No. AMPD1-1KT Heart Infusion (HI) Agar,Becton Dickson,Catalog No. 244400 Lysostaphin,Ambi

(17) Protocol: A Multiplexed Reporter Assay to Study Effects of Chromatin Context on DNA Double-Strand Break RepairFrontiers in GeneticsJanuary 31, 2022Ruben Schep, Christ Leemans, Eva K. Brinkman, Tom van Schaik, Bas van SteenselR3131S/L—20 U/µl)8. KpnI-HF® (New England BioLabs—Cat#: R3142S/L—20 U/µl)9. BssHII (New England BioLabs—Cat#: R0199S/L—5 U/µl)10. MluI-HF (New England BioLabs—Cat#: R0198S/L—20 U/µl)11. Ampicilin (brand not critical)12.

(18) Drug-like antibodies with high affinity, diversity and developability directly from next-generation antibody librariesmAbsDecember 1, 2021Andre Azevedo Reis Azevedo Reis Teixeira et al.used Q5 polymerase. The scFv amplicons were digested using BssHII and NheI restriction enzymes (NEB, #R0199L and #R0131L) and ligated to the pDAN5 phagemid vector22 previously digested with the same enzymes. Ligation

(19) Controlling for activity‐dependent genes and behavioral states is critical for determining brain relationships within and across speciesJournal of Comparative NeurologyAugust 1, 2021Matthew T. Biegler, Lindsey J. Cantin, Danielle L. Scarano, Erich D. Jarvisthe plasmid with PvuII‐HF (New England Biolabs, Cat. #R3151) or BssHII (New England Biolabs, Cat. #R0199) restriction enzymes, targeting restriction sites flanking the probe sequence. The cDNA products were

(20) Construction, characterization and crystal structure of a fluorescent single-chain Fv chimeraProtein Engineering, Design and SelectionFebruary 15, 2021Nileena Velappan, Devin Close, Li-Wei Hung, Leslie Naranjo, Colin Hemez, Natasha DeVore, Donna K McCullough, Antonietta M Lillo, Geoffrey S Waldo, Andrew R M Bradburygenes were cloned into the vector systems described below, using restriction enzymes BssH II and Nhe I (R0199 and R3131, New England Biolabs). The scFv and scTGP genes were both cloned into pEP, a periplasmic expression

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BssHII

BssHII from New England Biolabs

Sequence

New England Biolabs

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BssHII from New England Biolabs

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