BsrDI from New England Biolabs

Product Specs
ItemBsrDI
CompanyNew England Biolabs
Price $72.00 Inquire
Catalog NumberR0574S
Quantity200 units
Concentration2 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

GCAAT(2/0)

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Integrative analysis of Poly(A)-seq and RNA-seq reveals transcriptional regulation of poly(A) tail length in tuberculosisMicrobiology SpectrumApril 7, 2026Bahetibieke Tuohetaerbaike, Jie Wang, Ying Li, Liya Yue, Peihan Wang, Cuidan Li, Hao Wen, Wenbao Zhang, Jing Wang, Fei Chen, et al.PCR products were purified by VAHTS DNA Clean Beads (Vazyme, N411-03) and then cut with BsrD1 (NEB, R0574S) to remove the 3′ end adaptor sequences, leaving the 3′ poly(A) tail sequence at the very end of the

(2) Precise, predictable genome integrations by deep-learning-assisted design of microhomology-based templatesNature BiotechnologyAugust 12, 2025Thomas Naert, Taiyo Yamamoto, Shuting Han, Ruth Röck, Melanie Horn, Philipp Bethge, Nikita Vladimirov, Fabian F. Voigt, Joana Figueiro-Silva, Ruxandra Bachmann-Gagescu, et al.with 5–10 nl of injection mix. Restriction digests of PCR products were performed with BsrDI (NEB, R0574S) overnight at 37 °C in NEB buffer r2.1 and with BtsI-v2 (NEB, R0667S) overnight at 37 °C in NEB rCutSmart. For

(3) Evaluation of the Association between LMTK3 Gene Polymorphism and Breast Cancer Risk in the Indian PopulationAsian Pacific Journal of Cancer PreventionJuly 1, 2025Siri C Gampa, Grace B Yriah, Sireesha V Garimellaand 5 m at 72˚C. Five units of the BsrDI restriction enzyme from New England Biolabs, Inc. (Cat. No.# R0574S) were used to digest a 15 µl aliquot of the amplified product at 37°C for an hour. The undigested PCR product

(4) Comprehensive benchmarking of genome editing quantification methods for plant applicationsiScienceApril 29, 2025Zheng Gong, Yan Zhang, Di Xia, Sohye Yoon, Peter Alexander Crisp, José Ramón Botella,New England Biolabs,R3733S NcoI-HF,New England Biolabs,R3193S BcoDI,New England Biolabs,R0542S BsrDI,New England Biolabs,R0574S BglII,New England Biolabs,R0144S MfeI-HF,New England Biolabs,R3589S RsaI,New England Biolabs,R0167S BsrDI,New

(5) Evaluation of the Association between LMTK3 Gene Polymorphism and Breast Cancer Risk in the Indian PopulationAsian Pac J Cancer PrevJanuary 1, 2025Siri C Gampa, Grace B Yriah, Sireesha V Garimellaand 5 m at 72˚C. Five units of the BsrDI restriction enzyme from New England Biolabs, Inc. (Cat. No.# R0574S) were used to digest a 15 µl aliquot of the amplified product at 37°C for an hour. The undigested PCR product

(6) Pythia: Non-random DNA repair allows predictable CRISPR/Cas9 integration and gene editingbioRxivSeptember 27, 2024Thomas Naert, Taiyo Yamamoto, Shuting Han, Melanie Horn, Phillip Bethge, Nikita Vladimirov, Fabian F. Voigt, Joana Figueiro-Silva, Ruxandra Bachmann-Gagescu, Fritjof Helmchen, et al.injection mix. Restriction digests of PCR products where performed with BsrDI (New England Biolabs, R0574S) overnight at 37°C in NEB buffer r2.1 and with BtsI-v2 (New England Biolabs, R0667S) overnight at 37°C

(7) Poly(A)-seq: A method for direct sequencing and analysis of the transcriptomic poly(A)-tailsPLOS ONEJune 16, 2020Fengyun Yu, Yu Zhang, Chao Cheng, Wenqing Wang, Zisong Zhou, Wenliang Rang, Han Yu, Yaxun Wei, Qijia Wu, Yi ZhangPCR products were purified by VAHTS DNA Clean Beads (Vazyme, N411-03) and then cut with BsrD1 (NEB, R0574S) to remove the 3’ end adaptor sequences, leaving the 3’ poly(A) tail sequence at the very end of the

(8) Large Inverted Duplications in the Human Genome Form via a Fold-Back MechanismPLoS GeneticsJanuary 30, 2014Karen E. Hermetz, Scott Newman, Karen N. Conneely, Christa L. Martin, Blake C. Ballif, Lisa G. Shaffer, Jannine D. Cody, M. Katharine RuddDNA (5 µg) from 18q-26C and a normal control was digested following the manufacturer's protocol (NEB #R0574S; 1 h at 65°C, 20 min at 80°C, and store at 4°C). Digested DNA was purified with a QIAquick Purification

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BsrDI

BsrDI from New England Biolabs

Sequence

New England Biolabs

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BsrDI from New England Biolabs

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