BspQI from New England Biolabs

Product Specs
ItemBspQI
CompanyNew England Biolabs
Price $317.00 Inquire
Catalog NumberR0712L
Quantity2500 units
Concentration10,000 units/ml
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

GCTCTTC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Poly(A) variants supporting robust transmission stability in bacteria and high protein expression in animals for mRNA in vitro transcriptionMolecular Therapy Nucleic AcidsMarch 12, 2026Hua Chen, Wei Qin, Hui Bao, Xuwei Chen, Xu Ye, Yi Wang, Liang Liu, Yanguang Zhang, Ying Sun, Tingting Zhang, et al.In vitro transcription For each IVT reaction, 20 μg of plasmid was linearized with 40 U of BspQ1 (NEB, Cat# R0712L) to generate template DNA ending with poly(A) tail. mRNA was synthesized by IVT using HighYield T7 RNA

(2) Brachyury‐Activated Fucoidan Hydrogel Microspheres Rejuvenate Degenerative Intervertebral Discs MicroenvironmentAdvanced ScienceSeptember 1, 2025Yuhao Gong, Wenxiao Shi, Xingzhu Liu, Hang Yu, Yinghui Wu, Yanzhang Xia, Caichun Yue, Chongkai Yang, Cong Shen, Renjun Pei, et al.commissioned VectorBuilder (Guangzhou, China) to synthesize plasmids. After the BspQI restriction enzyme (NEB, R0712L) treatment, the linearized plasmid was purified with the Monarch PCR & DNA Cleanup Kit (NEB, T1030L).

(3) PALB2 and 53BP1 govern post-resection homologous recombination DNA repairMolecular CellAugust 7, 2025Yifan Wang, Yan Coulombe, Wei-Ting Yueh, Gregory D. Conway, Larissa Milano, David J. Glass, Mélissa Thomas, Amélie Rodrigue, Joseph Nacson, Dongbo Lu, et al.proteins" Rucaparib,Clovis Oncology,N/A Cas9 nuclease,New England Biolabs,Cat# M0386S BspQ1,New England Biolabs,Cat# R0712L Vectashield mounting medium with DAPI,Vector,Cat# H-1200 5-Bromo-2’-deoxyuridine (BrdU),Millipore Sigma

(4) Sortilin‐Mediated Rapid, Precise and Sustained Degradation of Membrane Proteins via mRNA‐Encoded Lysosome‐Targeting ChimeraAdvanced ScienceApril 30, 2025Xin Chang, Xinyu Qiu, Xiaoning Tong, Shaoju Gan, Weicheng Yi, Sitao Xie, Xiangsheng Liu, Chao Zuo, Weihong TanIn Vitro Transcription and mRNA Purification Plasmids were linearized using BspQ I (NEB, catalog no. R0712L) and subsequently used as DNA templates for in vitro transcription (IVT) with T7 RNA polymerase and

(5) An mRNA vaccine induces antimycobacterial immunity by activating DNA damage repair and autophagyMolecular Therapy - Nucleic AcidsMarch 11, 2025Dan Chen, Weili Huang, Lifang Shen, Junli Zhang, Zhifen Pan, Chen Zhang, Yuting Tang, Ziwei Zhou, Jie Tao, Geyang Luo, et al.codon-optimized L3 protein. The plasmid was linearized using the restriction endonuclease BspQI (NEB, catalog no. R0712L). mRNA was transcribed to contain a 75-nt-long poly(A) tail, with 100% of uridine triphosphate (UTP)

(6) Optimal Chimeric Antigen Receptor (CAR)-mRNA for Transient CAR T Cell GenerationInternational journal of molecular sciencesJanuary 23, 2025Kitte R, Serfling R, Blache U, Seitz C, Schrader S, Köhl U, Fricke S, Bär C, Tretbar US.72 °C (final extension). Optionally, the obtained PCR product was digested with 10 units of BspQI (#R0712L, New England Biolabs, Ipswich, MA, USA) per 1 µg DNA. To produce the DNA template for IVT using pDNA

(7) Protocol for precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-CasSTAR ProtocolsMarch 15, 2024Adrian Pascal Nievergelt, Dennis Ray Diener, Aliona Bogdanova, Thomas Brown, Gaia PiginoPlus,Biotium,31077-T GelGreen,Biotium,41005 Betaine,Merck,61962 NEBuilder HiFi assembly master mix,NEB,E2621L BspQI,NEB,R0712L BtsI-v2,NEB,R0667L SacI-HF,NEB,R3156L EcoRI-HF,NEB,R3101L NheI-HF,NEB,R3131S Hutner’s trace elements,Chlamydomonas

(8) A single immunization with core–shell structured lipopolyplex mRNA vaccine against rabies induces potent humoral immunity in mice and dogsEmerging Microbes & InfectionsDecember 8, 2023Jiawu Wan, Jianmei Yang, Zongmei Wang, Ruizhong Shen, Chengguang Zhang, Yuntao Wu, Ming Zhou, Huanchun Chen, Zhen F. Fu, Haiwei Sun, et al.(GenBank: GU565703). The plasmid was linearized using the restriction endonuclease BspQI (NEB, Cat.no.R0712L). mRNA was transcribed to contain a 75 nucleotide-long poly(A) tail, with 100% of UTP substituted with

(9) Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-CasCell Reports MethodsAugust 22, 2023Adrian Pascal Nievergelt, Dennis Ray Diener, Aliona Bogdanova, Thomas Brown, Gaia PiginoPlus,Biotinum,31077-T GelGreen,Biotinum,41005 Betaine,Merck,61962 NEBuilder HiFi assembly MM,NEB,E2621L BspQI,NEB,R0712L SacI-HF,NEB,R3156L EcoRI-HF,NEB,R3101L NheI-HF,NEB,R3131S Hutner’s Trace Elements,Chlamydomonas

(10) Structural Characterization of a Neutralizing Nanobody With Broad Activity Against SARS-CoV-2 VariantsFrontiers in MicrobiologyJune 2, 2022Tingting Li et al.the pDX_init vector (Zimmermann et al., 2018) were digested separately with 50 units of BspQI (Cat. R0712L, New England Biolabs) for 1.5 h at 50°C before heat inactivation at 80°C for 10 min. The digested DNA

(11) Isolation, characterization, and structure-based engineering of a neutralizing nanobody against SARS-CoV-2International Journal of Biological MacromoleculesJune 1, 2022Tingting Li, Bingjie Zhou, Yaning Li, Suqiong Huang, Zhipu Luo, Yuanze Zhou, Yanling Lai, Anupriya Gautam, Salome Bourgeau, Shurui Wang, et al.gel-purified PCR product and 10 μg of the pDX_init vector [25] were digested separately with BspQI (Cat. R0712L, New England Biolabs) at 50 °C. After 1.5 h, the mixture was heated at 80 °C for 10 min to inactivate

(12) RNAcompete methodology and application to determine sequence preferences of unconventional RNA-binding proteinsMethodsApril 1, 2017Debashish Ray, Kevin C.H. Ha, Kate Nie, Hong Zheng, Timothy R. Hughes, Quaid D. Morris(165-3301) Bromophenol Blue (BB),BioShop (BRO777.10) BSA Standard (2 mg/mL),Pierce (23209) BspQI,NEB (R0712L) C41 E.coli competent cells,Lucigen Corporation (60442-1) Coomassie Brilliant Blue,BioShop (CBB555.25) dNTPs,Thermo Scientific

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BspQI

BspQI from New England Biolabs

Description

Sequence

New England Biolabs

Citations (12)

Reviews

BspQI from New England Biolabs

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