BspHI from New England Biolabs

Product Specs
ItemBspHI
CompanyNew England Biolabs
Price $75.00 Inquire
Catalog NumberR0517S
Quantity500 units
Concentration10,000 units/ml
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

T/CATGA

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Enhanced editing of Bifidobacterium lactis using the endogenous Type I-G CRISPR-Cas systemApplied and Environmental MicrobiologyFebruary 18, 2026Ourania Raftopoulou, Kendall Malmstrom, Meichen Pan, Rodolphe Barrangouediting templates were then cloned into BspHI-digested screening vectors (NEB, Ipswich, MA, USA; Cat# R0517S) using NEBuilder HiFi Assembly Master Mix (NEB, Ipswich, MA, USA; Cat# E2621S), yielding the final CRISPR-editing

(2) Concurrent D-loop cleavage by Mus81 and Yen1 yields half-crossover precursorsNucleic Acids ResearchJuly 8, 2024Raquel Carreira, Tomas Lama-Diaz, Maria Crugeiras, F Javier Aguado, Marek Sebesta, Lumir Krejci, Miguel G Blancoindicated nuclease (as described), was followed by cleavage with 10 U BspHI (New England Biolabs, #R0517S) and incubated at 37ºC for 20 min followed by dephosphorylation with 1 U Shrimp Alkaline Phosphatase (rSAP

(3) Alternative polyadenylation alters protein dosage by switching between intronic and 3'UTR sitesScience advancesFebruary 17, 2023de Prisco N, Ford C, Elrod ND, Lee W, Tang LC, Huang KL, Lin A, Ji P, Jonnakuti VS, Boyle L, et al.microliters of the 666-bp PCR product was digested with 0.3 μl of Bsph I (New England Biolabs, catalog no. R0517). The C>T mutation creates a Bsph I restriction site; the PCR product from wild-type zebrafish will

(4) Plasmid-DNA Delivery by Covalently Functionalized PEI-SPIONs as a Potential 'Magnetofection' AgentMoleculesNovember 1, 2022RenÃ© Stein, Felix Pfister, Bernhard Friedrich, Pascal-Raphael Blersch, Harald Unterweger, Anton Arkhypov, Andriy Mokhir, Mikhail Kolot, Christoph Alexiou, Rainer Tietzeusing the BspHI enzyme following the manufacturers’ instructions (New England BioLabs, Product No. R0517S). A volume of 2 µL of 6xPPP loading dye were mixed with 10 µL of each sample. The samples consisted

(5) Identification of an integrase-independent pathway of retrotranspositionScience AdvancesJuly 1, 2022Feng Li, Michael Lee, Caroline Esnault, Katie Wendover, Yabin Guo, Paul Atkins, Mikel Zaratiegui, Henry L. Levin16°C, followed by 15 min at 65°C. Spe I/BspH I digestion Spe I (8 μl; NEB, R3133L) or BspH I (NEB, R0517L), 24 μl of 10× NEB Cutsmart buffer (NEB, B7204S), and 208 μl of ddH2O were added to the 160-μl ligation

(6) pRB-Depleted Pluripotent Stem Cell Retinal Organoids Recapitulate Cell State Transitions of Retinoblastoma Development and Suggest an Important Role for pRB in Retinal Cell DifferentiationStem Cells Translational MedicineApril 29, 2022Agata Rozanska et al.grown in 24-well plates to be assessed for gene editing with restriction enzyme assay (BspHI, NEB, R0517L). Selected clones were sequenced to confirm the introduction of the mutation to the wild-type allele

(7) Efficient genome editing of an extreme thermophile, Thermus thermophilus, using a thermostable Cas9 variantScientific ReportsDecember 1, 2021Bjorn Thor Adalsteinsson, Thordis Kristjansdottir, William Merre, Alexandra Helleux, Julia Dusaucy, Mathilde Tourigny, Olafur Fridjonsson, Gudmundur Oli Hreggvidssonµg/ml) and plasmids miniprepped (NEB, T1010). The miniprepped plasmids were restricted with BspHI (NEB, R0517) and separated on agarose gel to confirm presence of inserts. Plasmids pTTCC_purA and pTTCC_crtI were

(8) Transposon insertion profiling by sequencing (TIPseq) for mapping LINE-1 insertions in the human genomeMobile DNADecember 1, 2019Jared P. Steranka, Zuojian Tang, Mark Grivainis, Cheng Ran Lisa Huang, Lindsay M. Payer, Fernanda O. R. Rego, Thiago Luiz Araujo Miller, Pedro A. F. Galante, Sitharam Ramaswami, Adriana Heguy, et al.no. BP2818500) (CAUTION Ethanol is highly flammable)AseI (NEB, cat. no. R0526S)BspHI (NEB, cat. no. R0517S)BstYI (NEB, cat. no. R0523S)HindIII (NEB, cat. no. R0104S)NcoI (NEB, cat. no. R0193S)PstI (NEB, cat.

(9) Engineered dCas9 with reduced toxicity in bacteria: implications for genetic circuit designNucleic Acids ResearchOctober 5, 2018Shuyi Zhang, Christopher A Voigttransformation, these plasmids were re-purified and digested with restriction enzyme BsphI (New England Biolabs, #R0517S) to make sure they have the expected sizes and thus rule out the possibility of unwanted homologous

(10) A simple PCR-based genotyping method for M105I mutation of alpha-SNAP enhances the study of early pathological changes in hyh phenotypeMolecular and Cellular ProbesJuly 7, 2009Luis Federico Bátiz, Ruth Roales-Buján, Luis M. Rodríguez-Pérez, Isabel M. Matas, Patricia Páez, María Roque, Antonio J. Jiménez, Cayo Ramos, José Manuel Pérez-Fígares

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BspHI

BspHI from New England Biolabs

Description

Sequence

New England Biolabs

Citations (10)

Reviews

BspHI from New England Biolabs

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