Nb.BsmI from New England Biolabs

Product Specs
ItemNb.BsmI
CompanyNew England Biolabs
Price $77.00 Inquire
Catalog NumberR0706S
Quantity1000 units
Concentration10,000 units/ml
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

5'…GAATGCN…3'
3'…CTTAC/GN...5'

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Control of DNA replication in vitro using a reversible replication barrierNature ProtocolsJuly 1, 2024Emma J. Vontalge, Tamar Kavlashvili, Steven N. Dahmen, Matthew T. Cranford, James M. Dewarphosphorylated, HPLC/PAGE-purified oligonucleotide containing the DNA adduct of interest Nb.BsmI (NEB, cat. no. R0706S) T4 DNA ligase (NEB, cat. no. M0202S) Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB, cat. no. T1030L)

(2) Electrophoretic Mobility Assay to Separate Supercoiled, Catenated, and Knotted DNA MoleculesBIO-PROTOCOLMay 5, 2024Jorge Cebrián, Victor Martínez, Pablo Hernández, Dora B. Krimer, María-Luisa Martínez-Robles, Jorge B. SCHVARTZMAN, María Jose Fernández-Nestosarecombinant (Roche, catalog number: 3115879001) Nb.BsmI 10,000 U/mL (New England Biolabs, catalog number: R0706S)Calcium chloride (Flinn Scientific, catalog number: C0234)Lambda DNA/HindIII Marker (Invitrogen, catalog

(3) Cytosine base modifications regulate DNA duplex stability and metabolismNucleic Acids ResearchDecember 16, 2021Cathia Rausch, Peng Zhang, Corella S Casas-Delucchi, Julia L Daiß, Christoph Engel, Gideon Coster, Florian D Hastert, Patrick Weber, M Cristina Cardoso28104, Qiagen, Hilden, Germany). Purified templates were cut with the nicking endonuclease Nb.BsmI (cat# R0706S, NEB, Ipswich, MA, USA) at 65°C for 2 h. The reaction was inactivated at 80°C for 20 min. After 10 min,

(4) Notch Intracellular Domain Plasmid Delivery via Poly(Lactic-Co-Glycolic Acid) Nanoparticles to Upregulate Notch Pathway MoleculesFrontiers in cardiovascular medicineJanuary 1, 2021Messerschmidt VL, Chintapula U, Kuriakose AE, Laboy S, Truong TTD, Kydd LA, Jaworski J, Pan Z, Sadek H, Nguyen KT, et al.used for analysis. For analysis, the plasmid solutions were incubated with Nb.Bsmi nicking enzyme (R0706S, New England Biolabs) for 60 min at 65°C in NEBuffer 3.1. The enzyme was then inactivated for 20 min

(5) Separable functions of Tof1/Timeless in intra-S-checkpoint signalling, replisome stability and DNA topological stressNucleic acids researchDecember 2, 2020Westhorpe R, Keszthelyi A, Minchell NE, Jones D, Baxter J.plasmid DNA catenation analysis of pRS316, purified DNA was nicked with Nb.Bsm1 (New England Biolabs, R0706S) according to manufacturer's instructions. For fork pausing analysis of pRS426-RFB, purified DNA was

(6) A teaching protocol demonstrating the use of EasyClone and CRISPR/Cas9 for metabolic engineering of Saccharomyces cerevisiae and Yarrowia lipolyticaFEMS Yeast ResearchSeptember 26, 2019N Milne, L R R Tramontin, I BorodinaFD0594)FastDigest SfaAI (AsiSI) (ThermoFisher scientific, MA, USA; FD2094)Nb.BsmI (New England Biolabs, MA, USA; R0706S)GeneRuler 1 Kb DNA Ladder, ready-to-use (ThermoFisher scientific, MA, USA; SM0313)50x TAE Buffer (ThermoFisher scientific

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Nb.BsmI

Nb.BsmI from New England Biolabs

Sequence

New England Biolabs

Citations (6)

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Nb.BsmI from New England Biolabs

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