BseRI from New England Biolabs

Product Specs
ItemBseRI
CompanyNew England Biolabs
Price $80.00 Inquire
Catalog NumberR0581S
Quantity200 units
Concentration4 u/µL
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

GAGGAG(10/8)

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Making GFP count: a validated framework for absolute protein quantification in precision fermentationAppl Microbiol BiotechnolFebruary 4, 2026Christina Peternell, Philipp Noll, Annette Brümmer-Rolf, Marius HenkelGenomics Germany GmbH Hamburg, Germany. The segment of interest was amplified, digested (R0655S PciI and R0581S BseRI, New England Biolabs GmbH, Frankfurt am Main, Germany), and ligated (T4 DNA Ligase EL0014, Thermo Scientific

(2) A CRISPR mis-insertion in the Zic3 5'UTR inhibits in vivo translation and is predicted to result in formation of an mRNA stem-loop hairpinBiology OpenMarch 15, 2025Helen M. Bellchambers, Maria B. Padua, Stephanie M. Wareremainder of the sequence was determined by cutting the PCR amplicon with the restriction enzyme BseRI (R0581S, New England Biolabs, Ipswich. MA, USA), which has a recognition site with the V5 tag. The resulting

(3) A large-scale cancer-specific protein–DNA interaction networkLife Science AllianceOctober 1, 2024Yunwei Lu, Anna Berenson, Ryan Lane, Isabelle Guelin, Zhaorong Li, Yilin Chen, Sakshi Shah, Meimei Yin, Luis Fernando Soto-Ugaldi, Ana Fiszbein, et al.Destination vectors were linearized using single-cutter restriction enzymes (R0520L, R0146L, R3127S, R0581S, R0193L, R0114S, R0187S, R0519L; New England Biolabs). Table S7. Gene promoter info. The pWM#2 and pWM#3

(4) Diindoles produced from commensal microbiota metabolites function as endogenous CAR/Nr1i3 ligandsNat CommunMarch 22, 2024Jiabao Liu, Ainaz Malekoltojari, Anjana Asokakumar, Vimanda Chow, Linhao Li, Hao Li, Marina Grimaldi, Nathanlown Dang, Jhenielle Campbell, Holly Barrett, et al.cloned into a pET28-MHL (GenBank accession EF456735) vector, which was first linearized by BseRI (NEB, R0581S) digestion. The human CAR LBD (Lys108 – Ser352) was amplified by PCR from cDNA with an N-terminal 15-bp

(5) Paired yeast one-hybrid assays to detect DNA-binding cooperativity and antagonism across transcription factorsNature CommunicationsOctober 18, 2023Anna Berenson, Ryan Lane, Luis F. Soto-Ugaldi, Mahir Patel, Cosmin Ciausu, Zhaorong Li, Yilin Chen, Sakshi Shah, Clarissa Santoso, Xing Liu, et al.were linearized with single-cutter restriction enzymes (New England Biolabs R0520L, R0146L, R3127S, R0581S, R0193L, R0114S, R0187S, R0519L). The pWM#2 and pWM#3 plasmids for each promoter were integrated simultaneously

(6) An HNF1α truncation associated with maturity-onset diabetes of the young impairs pancreatic progenitor differentiation by antagonizing HNF1β functionCell reportsMarch 1, 2022Cujba AM, Alvarez-Fallas ME, Pedraza-Arevalo S, Laddach A, Shepherd MH, Hattersley AT, Watt FM, Sancho R.gelatin,Sigma,G7765-1L BbsI restriction enzyme,NEB,R3539S AgeI restriction enzyme,NEB,R3552S BseRI restriction enzyme,NEB,R0581S BamHI restriction enzyme,NEB,R3136S EcoRI restriction enzyme,NEB,R3101S T4 DNA ligase,NEB,M0202 PVDF

(7) Integrated single-cell sequencing of 5-hydroxymethylcytosine and genomic DNA using scH&G-seqSTAR ProtocolsDecember 24, 2021Alex Chialastri, Chatarin Wangsanuwat, Siddharth S. DeyCA-630,Sigma-Aldrich,I8896-50ML Recombinant Ribonuclease Inhibitor,Clontech,2313A AluI,New England BioLabs,R0137S BseRI,New England BioLabs,R0581S Potassium acetate solution,Sigma-Aldrich,95843-100ML-F Trizma acetate (Tris-acetate),Sigma-Aldrich,93337-25G Magnesium

(8) Redox-sensitive CDC-42 clustering promotes wound closure in C. elegansCell ReportsNovember 23, 2021Jingxiu Xu et al.II,Invitrogen,Cat#11791–020 10X KLD Enzyme Mix,NEB,Cat#M0554S T4 DNA Ligase,NEB,Cat#M0202L BseR I,NEB,Cat#R0581S ,, Deposited data,Deposited data,Deposited data MS Proteomics Data,This paper,ProteomeXchange: PASS01707 Supplementary

(9) A probabilistic framework for cellular lineage reconstruction using integrated single-cell 5-hydroxymethylcytosine and genomic DNA sequencingCell Reports MethodsAugust 23, 2021Chatarin Wangsanuwat, Alex Chialastri, Javier F. Aldeguer, Nicolas C. Rivron, Siddharth S. Dey,New England BioLabs,M0541L IGEPAL CA-630,Sigma-Aldrich,I8896-50ML AluI,New England BioLabs,R0137S BseRI,New England BioLabs,R0581S ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, MEGAscript T7 Transcription Kit

(10) Ultrasensitive deletion detection links mitochondrial DNA replication, disease, and agingGenome BiologyDecember 1, 2020Scott A. Lujan et al.10,014–12,405. A second deletion plasmid was generated using BseRI (New England Biolabs Inc., Catalog # R0581S), which cut at positions 9323 and 12,974 to generate a 3652 bp deletion. A third control plasmid generated

(11) Proteome Instability Is a Therapeutic Vulnerability in Mismatch Repair-Deficient CancerCancer cellMarch 16, 2020McGrail DJ, Garnett J, Yin J, Dai H, Shih DJH, Lam TNA, Li Y, Sun C, Li Y, Schmandt R, et al.12605010 Bgll,New England Biolabs,Cat# R0144S Nsil,New England Biolabs,Cat# R0127S BseR1,New England Biolabs,Cat# R0581S Critical Commercial Assays,Critical Commercial Assays,Critical Commercial Assays Lipofectamine 3000,Thermo Fisher Scientific

(12) Viral Satellites Exploit Phage Proteins to Escape Degradation of the Bacterial Host ChromosomeCell Host & MicrobeOctober 9, 2019Amelia C. McKitterick, Stephanie G. Hays, Fatema-Tuz Johura, Munirul Alam, Kimberley D. Seed41005 GelRed,Biotium,Cat# 41003 BsaI,New England Biolabs,Cat# R0535S BseRI,New England Biolabs,Cat# R0581S T4 Ligase,New England Biolabs,Cat# M0202S EcoRV-HF,New England Biolabs,Cat# R3195S SalI-HF,New England Biolabs

(13) c.+62G>A and g.-420C>G RETN Polymorphisms and the Risk of Developing Type 2 Diabetes and Obesity: Original Research on a Mexican Population and Meta-analysisEndocrinology & Metabolic SyndromeJanuary 1, 2016Pablo A. Montiel-Tellez BS, Adriana Nieva-Vazquez249 bp PCR product. The PCR products were digested overnight using BseRI restriction enzyme (Catalog #R0581S, New England BioLabs Inc.). The A allele, after digestion, would produce two fragments (238 and 11 bp),

(14) Paired-end diTagging for transcriptome and genome analysisCurrent protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]July 1, 2007Patrick Ng, Chia-Lin Wei, Yijun Ruan

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Paired- End diTagging for Transcriptome and Genome Analysis
July 01, 2007

BseRI

BseRI from New England Biolabs

Sequence

New England Biolabs

Citations (14)

Reviews

Protocols

Paired- End diTagging for Transcriptome and Genome Analysis

BseRI from New England Biolabs

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