RQ1 RNase-Free DNase from Promega

Product Specs
ItemRQ1 RNase-Free DNase
CompanyPromega
Price $695.00Supplier Page View Company Product Page
Catalog NumberM6101
Quantity1,000u
Concentration1 U/µl
Molecular Weight~68kDa

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to completely degrade 1 µg of DNA in 10 minutes at 37°C in 50 µl of a buffer containing 40mM Tris-HCl (pH 7.9 at 25°C), 10mM NaCl, 6mM MgCl2, 10mM CaCl2.

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Overexpression of Circ_0004496 is Associated with Pathological Bone Formation in Ankylosing Spondylitis via the miR-145/ACTG1 AxisBalkan Medical JournalApril 30, 2026Yu-Cong Zou, Yong-Sheng Wu, Ting Hu, Hao-Bin Zenggenomic DNA (gDNA) contamination, purified RNA was treated with RQ1 RNase-free DNase I (Promega, Cat. No. M6101). qRT-PCR was performed using SYBR Premix DimerEraser (Takara, Dalian, China). Relative gene expression

(2) Phosphatidylinositol transfer protein-1 integrates insulin/IGF-1 and TOR signaling to negatively regulate lifespan and healthspan in Caenorhabditis elegansJ Biomed SciApril 27, 2026Yen-Hung Lin, Yun-Hsun Liao, Sin-Bo Liao, Tzu-Yu Lin, Muniesh Muthaiyan Shanmugam, Pei-Jia Hsu, Chang-Shi Chen, Tsiu-Ting Ching, Oliver Ingvar Wagner, Chiou-Hwa Yuh, et al.adding isopropanol (VWR, 0918). Extracted RNA was further purified by RQ1 RNase-Free DNase (Promega, # M6101). 1 µg of total RNA added with random primer (Promega, C118A) and M-MLV reverse transcriptase (Promega,

(3) MYC-bound enhancer RNAs in cis regulate gene transcription and tumorigenesisSci AdvApril 24, 2026Sihan Li, Zehua Wang, Phuong Nguyen, Yue Wang, Yueshan Zhao, Yifei Wang, Yuang Chen, Haozhe Huang, Xiaofei Wang, Yu Zhang, et al.15596026). For eRNA analysis, 10 μg of total RNA was digested with RNase-free deoxyribonuclease I (Promega, M6101) for 30 min at 37°C, and RNA cleanup was performed using the RNA Clean and Concentrator-5 columns (Zymo Research

(4) Bioengineered iPSC-derived human macrophages with increased angiotensin-converting enzyme (ACE) expression suppress solid tumor growthSignal Transduct Target TherApril 13, 2026Tomohiro Shibata, Shabir Bhat, DuoYao Cao, Suguru Saito, Noor Khan, Ellen A. Bernstein, Takeshi Tokudome, Derick Okwan-Duodu, Warren G. Tourtellotte, Kenneth E. Bernstein, et al.1.5 mg/ml collagenase IV (Worthington Biochemical Corp., LS004182) and 0.1 mg/ml DNase I (Promega, M6101) at 37 °C. Collagenase was neutralized with RPMI medium supplemented with 10% FBS and 1 mM EDTA. After

(5) Lipidomic Analysis Reveals Drug-Induced Lipoxin Synthesis in Glaucoma TreatmentJCI InsightApril 8, 2026David J. Mathew, Shubham Maurya, Julian Ho, Izhar Livne-Bar, Darren Chan, Jenny Wanyu Zhang, Yvonne M. Buys, Marisa Sit, Graham Trope, Donna M. Peters, et al.the manufacturer’s instructions. RNA samples were treated with RNase-free DNase (Promega RQ1 kit, PR-M6101). RNA purity was assessed using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, ND-2000),

(6) Adenovirus phagocytosis by neutrophils triggers a pro-inflammatory responsePLoS PathogApril 6, 2026Salomé Laurans, Soizic Huerre, Olivier Dellis, Céline Férard, Hadrien Jalaber, Clément Vanbergue, Emilie Brun, Rémy Jelin, Oliver Nüsse, Karim Benihoud, et al.Tissue RNA Isolation kit (Biotum CD504) and treated with a DNAse (RQ1 RNAse-free DNAse kit, PROMEGA, M6101). RT-qPCR was performed with GoTaq 2 step RT-qPCR kit (PROMEGA, A6010). qPCR primer sequences (Biomol

(7) NADPH-producing enzymes restrict the formation of pancreatic precancerous lesionsNat MetabApril 1, 2026Megan D. Radyk, Barbara S. Nelson, Mariana Tannus Ruckert, Christopher J. Halbrook, Mengrou Shan, Jonathan M. Alektiar, Brooke L. Lavoie, Lucie Salvatore, Wei Yan, Matthew D. Perricone, et al.Mini Kit (Qiagen, 74104) according to the manufacturer’s instructions. RQ1 RNase-Free DNase (Promega, M6101) was added to samples, and then cDNA was generated using iScript Reverse Transcription Supermix (Bio-Rad,

(8) The human branchpoint-interacting stem-loop sequence and structure regulates U2 snRNA expression, branchpoint recognition, and the transcriptomeNucleic Acids ResMarch 26, 2026Meredith B Stevers, Sol Katzman, Melissa S Juricabiological replicates. Reverse transcription Total RNA was treated with RQ1 DNase (Promega, cat. M6101) followed by phenol:chloroform:iso-amyl alcohol (25:24:1 v/v) extraction, chloroform:iso-amyl alcohol

(9) Elucidating the mechanism of thiosulfate oxidation by a novel two-component system SulRS in deep-sea CampylobacterotaiScienceMarch 20, 2026Yangsheng Zhong, Shasha Wang, Guangshan Wei, Xuewen Gao, Chen Yang, Xiaoqun Nie, Karine Alain, Rongfeng Hong, Xiang Zeng, Zongze Shao, et al.phosphate,Sangon,ZB63E2T Ampicillin,Solarbio,A8180 Kanamycin,MACKLIN,K812217 4S Red Plus nucleic acid stain,Sangon,A606695 RQ1 RNase-Free DNase,Promega,M6101 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, HiScript

(10) Central nervous system pericytes express soluble ST2 in inflammation and injuryMolecular BrainMarch 19, 2026Deidre Jansson, Blake Highet, Susan Li, Taylor J. Stevenson, Leon C. D. Smyth, Justin Rustenhoven, Sheryl Feng, Richard Daneman, Cayce Dorrier, Andrew Clarkson, et al.manufacturer’s instructions. RNA was quantified using Nanodrop. cDNA was made from 2–3 µg DNase-1 (Promega, M6101)-treated RNA using the Superscript IV first strand synthesis kits (Invitrogen, 18090010). PCR was performed

(11) LENG8 mediates RNA nuclear retention and degradation in eukaryotesMol CellMarch 19, 2026Lusong Tian, Liang Liu, Yoseop Yoon, Lindsey V. Soles, Marielle Valdez, Joshua Jeong, Clinton Yu, Lan Huang, Yongsheng ShiLine 4D-Nucleofector,ThermoFisher,Cat#NC0281111 X Kit,Scientific, RQ1 RNase-Free DNase,Promega,Cat#M6101 Superscript III Reverse Transcriptase,ThermoFisher Scientific,Cat#18080044 Random hexamers,ThermoFisher

(12) The E3 ubiquitin ligase mechanism specifying targeted microRNA degradationNatureMarch 18, 2026Jakob Farnung, Elena Slobodyanyuk, Peter Y. Wang, Lianne W. Blodgett, Daniel H. Lin, Susanne von Gronau, Brenda A. Schulman, David P. BartelNEB, M0296). After incubation at 37 °C for 3–4 h, DNA templates were digested with RQ1 DNase (Promega, M6101) at 37 °C for 30 min. RNA products were then gel-purified on a urea-polyacrylamide gel. Gel-purified

(13) Mechanistic dissection of GRHL2 and PR transcriptional co-regulation in breast cellsPLoS GenetMarch 17, 2026Marleen T. Aarts, Anna Nordin, Claudio Cantù, Antonius L. van Boxtel, Renée van Amerongen#15596018) following the manufacturer’s protocol. Isolated RNA was treated with RQ1 DNase (Promega, #M6101) to remove genomic DNA contamination. cDNA synthesis was carried out using SuperScript IV Reverse Transcriptase

(14) Interdependent RNA structural motifs at the 3ʹ-terminus of the West Nile virus genome regulate viral growthbioRxivMarch 14, 2026Lucille H. Tsao, Doug E. Brackney, Anna Marie Pyletranscribed by T7 RNA polymerase P266L variant (39), followed by RQ1 DNase treatment (Promega, cat #M6101) and 5 ul of 0.5 M EDTA (pH 8) to chelate Mg2+. Transcribed RNA was further concentrated on 50 kDa Amicon

(15) m6A reader Ythdf proteins control retrotransposon B2 repeat expression and safeguard early embryo developmentThe EMBO JournalMarch 11, 2026Ruibao Su, Di Gao, Zongchang Du, Tie-Gang Meng, Chao Li, Lei-Ning Chen, Xiaoting Lin, Changchang Cao, Li-Hua Fan, Yanbin Dong, et al.for 10 min. Then, 1 µl of RNase inhibitor (Thermo Scientific, EO0381) and 4 µl of RQ1 DNase (Promega, M6101) were added, and the mixture was then incubated at 37 °C for 3 min. After snap-chilling the tube on

(16) DNA damage and cell death induced by exposure to ultra-high dose rate low-dose pulsed X-rays emitted from a kilojoule plasma focus deviceBiological ResearchMarch 10, 2026Héctor Araya, Jalaj Jain, Rodrigo Andaur, José Moreno, Sergio Davis, Pablo Diaz, Ethel Velásquez, Josefa Orellana, Martín Ríos, Jessica Toro, et al.(Cytation 3™, BioTek instruments). Subsequently, RNA was treated with RQ1 RNase-Free DNase (Promega, cat: M6101) for 30 minutes at 37°C to remove genomic DNA contamination. cDNA was synthetized using Affinity Script

(17) The Lsm1-7 complex couples 3'-end protection to histone H4 acetylation to maintain mRNA homeostasis in Fusarium graminearumNucleic Acids ResMarch 10, 2026Yiyi Ren, Jiayue Yan, Xingmin Han, Chenghui Xu, Meiling Guo, Xuan Wang, Chao Liu, Antonio F Logrieco, Yongfeng Jin, Zhonghua Ma, et al.with shaking for 24 h. Total RNA was extracted using standard protocols and treated with RQ1 DNase (M6101, Promega, Madison, WI, USA) to remove genomic DNA. Partial digestion was performed with RNase T1 (EN0541,

(18) Mitochondrial redox homeostasis links organellar stress surveillance to germline and somatic integrity in Caenorhabditis elegansRedox BiologyMarch 9, 2026Marina Valenzuela-Villatoro, Patricia de la Cruz-Ruiz, David Guerrero-Gómez, Eva Gómez-Orte, Alfonso Schiavi, Silvia Maglioni, Mayte Montero, Rosalba I. Fonteriz, José Carlos Casas-Martínez, Nicole E. Briand, et al.each sample, a total reaction of 10 μl contained: 500 ng RNA, 1 μl RNase-Free DNAse (Promega, Cat. #M6101), 1 μl 10x Reaction Buffer and DEPC water. Reactions were incubated for 30 min at 37 °C and then stopped

(19) Control of retrotransposon-driven activation of the interferon response by the double-stranded RNA binding protein DGCR8Nucleic Acids ResMarch 5, 2026Ana Gázquez-Gutiérrez, Priscilla Chin, Guillermo Peris, Katrina Gordon, Pilar G Marchante, Jeroen Witteveldt, Pablo Tristán-Ramos, Jerome O Rouvière, Lisanne I Knol, Alasdair Ivens, et al.following manufacture’s instructions. RNA was subjected to initial DNase treatment using RQ1 DNase (M6101, Promega) for one hour at 37°C, followed by phenol/chloroform purification and RNA quantification. One

(20) Botulinum toxin-induced masseter muscle atrophy is associated with impaired autophagic flux without signs of apoptosis in miceCell Death DiscoveryFebruary 28, 2026Esteban R. Quezada, Noelia Blanco, Paola Llanos, Alfredo Criollo, Mario Chiong, Sonja Buvinic(Invitrogen, S36939) and stored at 4 °C until use. The DNAase kit was a positive control (Promega, M6101, WI, USA). Images were acquired with an inverted epifluorescence microscope (Ti-E, Nikon®, USA) using

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