BlpI from New England Biolabs

Product Specs
ItemBlpI
CompanyNew England Biolabs
Price $329.00 Inquire
Catalog NumberR0585L
Quantity2500 units
ApplicationsDNA Manipulation
Concentration10 u/µL
TypeRestriction Enzyme
Temperature-20 C

Sequence

GC/TNAGC

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) CRISPR activation screens map the genomic landscape of cancer glycome remodelingCell GenomicsApril 8, 2026John Daly, Lidia Piatnitca, Mohammed Al-Seragi, Vignesh Krishnamoorthy, Simon Wisnovskysolution),InvivoGen,ant-bl-05 RetroNectin-coated plates,Takara Bio,T100A BstXI,New England Biolabs,R0113L BlpI,New England Biolabs,R0585L T4 DNA Ligase,New England Biolabs,M0202S ,, Critical commercial assays,Critical commercial assays,Critical

(2) Integrative analysis of mRNA stability regulation uncovers a metastasis-suppressive program in breast cancerSci AdvMarch 13, 2026Heather Karner, Tabea C. Mittmann, Vicky W. Chen, Ashir A. Borah, Andreas Langen, Hassan Yousefi, Lisa Fish, Balyn W. Zaro, Albertas Navickas, Hani Goodarzifrom pJR89 (Addgene, 140096) (26) using two-step cloning first with BstXI (NEB, R0113S) and BlpI (NEB, R0585S) and second with BsmBI-v2 (NEB, R0739S). The sgRNA sequences were selected from (36). See table S5 for

(3) Insights from pooled CRISPRi single-cell screens in K562 cells reveal gene functions, regulatory networks, and highlight opportunities and limitationsBMC GenomicsMarch 13, 2026Hong Zhang, Peifen Zhang, Eric Bindels, Eskeatnaf Mulugetawas prepared by digesting the pBA950 plasmid (Addgene, plasmid #122239) with BlpI (NEB, catalog no. R0585S) and BstXI (NEB, catalog no. R0113S) in NEBuffer r2.1 at 37 °C for 1 hour. After subjecting the digested

(4) Reduced Cas9 transgene silencing by incorporation of intron sequencesNature communicationsNovember 27, 2025Arana S, Du PP, Vaughan-Jackson A, Enright N, Spees K, Valbuena R, Garcia CA, Nguyen T, Venida A, Seczynska M, et al.using a double BstXI and BlpI digest (BstXI: New England Biolabs R0113S, BlpI: New England Biolabs R0585S) and the guide oligo was ligated using T4 DNA ligase (New England Biolabs M0202S). The HBB IVS2 intron

(5) Toward optimizing diversifying base editors for high-throughput mutational scanning studiesNucleic Acids ResJuly 4, 2025Carley I Schwartz, Nathan S Abell, Amy Li, Aradhana, Josh Tycko, Alisa Truong, Stephen B Montgomery, Gaelen T HesspGH643 was incubated overnight at 37°C [10 μg pGH643, 4.5 μl Esp3I (NEB, R0734L), 4.5 μl BlpI (NEB, R0585L), and 10 μl of CutSmart Buffer (NEB)]. The digest reaction was run on a 1% TAE gel, and the band was

(6) Integrative analysis of mRNA stability regulation uncovers a metastasis-suppressive program in breast cancerbioRxivJune 7, 2025Heather Karner, Tabea Mittmann, Vicky W. Chen, Ashir A. Borah, Andreas Langen, Hassan Yousefi, Lisa Fish, Balyn W. Zaro, Albertas Navickas, Hani Goodarzifrom pJR89 (Addgene, 140096)27 using two-step cloning first with BstXI (NEB, R0113S) and BlpI (NEB, R0585S) and second with BsmBI-v2 (NEB, R0739S). The sgRNA sequences were selected from Horlbeck et al. (2016,

(7) Elucidating the genetic mechanisms governing cytosine base editing outcomes through CRISPRi screensNat CommunMay 20, 2025Sifeng Gu, Zsolt Bodai, Rachel A. Anderson, Hei Yu Annika So, Quinn T. Cowan, Alexis C. Komorcloned into the corresponding reporter vectors (pSG298 or pSG299) via BstXI/BlpI (BstXI: R0113L, BlpI: R0585L, NEB) restriction enzyme cloning to produce single CRISPRi Sp-sgRNA vectors (Supplementary Data 5).

(8) A hierarchical pathway for assembly of the distal appendages that organize primary ciliaeLifeJanuary 30, 2025Tomoharu Kanie, Beibei Liu, Julia F Love, Saxton D Fisher, Anna-Karin Gustavsson, Peter K Jacksonoligonucleotides encoding sgRNAs into the pMCB306 vector digested with BstXI (R0113S, NEB) and BlpI (R0585S, NEB) restriction enzymes. Before ligation, 4 µM of forward and reverse oligonucleotides listed in ‘Source

(9) Myristoylated Neuronal Calcium Sensor-1 captures the preciliary vesicle at distal appendageseLifeJanuary 30, 2025Tomoharu Kanie, Roy Ng, Keene L Abbott, Niaj Mohammad Tanvir, Esben Lorentzen, Olaf Pongs, Peter K Jacksonoligonucleotides encoding sgRNAs into 25 ng of the pMCB306 vector digested with BstXI (R0113S, NEB) and BlpI (R0585S, NEB) restriction enzymes along with 0.25 µl of T4 ligase (M0202S, NEB) in 2.5 µl total reaction volume.

(10) Long-range regulation of transcription scales with genomic distance in a gene-specific mannerMolecular CellJanuary 16, 2025Christina L. Jensen, Liang-Fu Chen, Tomek Swigut, Olivia J. Crocker, David Yao, Mike C. Bassik, James E. Ferrell, Alistair N. Boettiger, Joanna WysockapMCB320 (https://www.addgene.org/89359/) using the restriction enzymes BstXI (NEB, R0113S) and BlpI (NEB, R0585S) and T4 DNA Ligase (NEB, M0202T). Ligation products were purified using QIAGEN MinElute columns (QIAGEN,

(11) Multiome Perturb-seq unlocks scalable discovery of integrated perturbation effects on the transcriptome and epigenomeCell SystemsJanuary 15, 2025Eli Metzner, Kaden M. Southard, Thomas M. Normaninhibitor,Sigma,Cat#3335402001 BstXI,New England Biolabs,Cat#R0113S BlpI,New England Biolabs,Cat#R0585S eBioscience™ 7-AAD Viability Staining Solution,ThermoFisher Scientific,Cat#00–6993-50 ,, Critical

(12) A benchmarked, high-efficiency prime editing platform for multiplexed dropout screeningNature methodsJanuary 1, 2025Cirincione A, Simpson D, Yan W, McNulty R, Ravisankar P, Solley SC, Yan J, Lim F, Farley EK, Singh M, et al.were aggregated and purified using SPRI. Vector backbone pAC026 was subjected to a BstXI-BlpI (NEB R0585S) double digest at 37 °C for 4 h followed by SPRI purification, BsmBI-v2 (NEB R0739S) digest at 55 °C

(13) CRISPRi/a screens in human iPSC-cardiomyocytes identify glycolytic activation as a druggable target for doxorubicin-induced cardiotoxicityCell stem cellDecember 5, 2024Liu C, Shen M, Liu Y, Manhas A, Zhao SR, Zhang M, Belbachir N, Ren L, Zhang JZ, Caudal A, et al.(WGA),Thermo Fisher Scientific,Cat#W11262 BstXI,New England Biolabs,Cat#R0113 BlpI,New England Biolabs,Cat#R0585 Critical commercial assays,Critical commercial assays,Critical commercial assays CytoTuneTM-iPS 2.0

(14) Mechanisms controlling replication fork stalling and collapse at topoisomerase 1 cleavage complexesMolecular CellSeptember 19, 2024Rose Westhorpe, Johann J. Roske, Joseph T.P. YeelesR0644 NheI-HF®,New England Biolabs,Cat# R3131 SmaI,New England Biolabs,Cat# R0141 BlpI,New England Biolabs,Cat# R0585 T4 PNK,New England Biolabs,Cat# M0201 Sepharose™ 4B,Sigma Aldrich,Cat# 4B200 T4 DNA Ligase,New England Biolabs

(15) Protocol for mass spectrometric profiling of lysine malonylation by lysine acetyltransferase in CRISPRi K562 cell linesSTAR ProtocolsJune 21, 2024Ran Zhang, Joanna Bons, Jacob P. Rose, Birgit Schilling, Eric Verdinproteins" ,, Acetonitrile (Burdick & Jackson; LC-MS grade),Honeywell,Cat. #LC015 BlpI,New England Biolabs,Cat. #R0585 BstXI,New England Biolabs,Cat. #R0113 DL-dithiothreitol (DTT),Sigma-Aldrich,Cat. #D9779 Formic acid

(16) CRISPRi/a Screening with Human iPSCsMethods in Molecular BiologyApril 13, 2024Masataka Nishiga, Lei S. Qi, Joseph C. Wu(pHRdSV40-scFv-GCN4-sfGFP-VP64-GB1-NLS) (Addgene #60904) BstX1 (Thermo Fisher Scientific, FD1024) Blp1 (New England Biolabs, R0585S) 2.3 Lentivirus production psPAX2 plasmid (Addgene #12260) pMD2.G plasmid (Addgene #12259) HEK

(17) Integrated multiplexed assays of variant effect reveal determinants of catechol-O-methyltransferase gene expressionMolecular Systems BiologyFebruary 14, 2024Ian Hoskins, Shilpa Rao, Charisma Tante, Can CenikZymoclean Gel DNA Recovery Kit,Zymo Research,D4007 ZymoPURE II Plasmid Maxiprep Kit,Zymo Research,D4203 BlpI,New England Biolabs,R0585 Q5 Site-Directed Mutagenesis Kit,New England Biolabs,E0554S Lipofectamine 3000,Thermo Fisher Scientific,L3000008 Doxycycline,Fisher

(18) Functional annotation of variants of the BRCA2 gene via locally haploid human pluripotent stem cellsNature Biomedical EngineeringJuly 24, 2023Hanqin Li, Rebecca Bartke, Lei Zhao, Yogendra Verma, Anna Horacek, Alma Rechav Ben-Natan, Gabriella R. Pangilinan, Netravathi Krishnappa, Rasmus Nielsen, Dirk Hockemeyervectors targeting exons 2 and 27 were purchased as oligo pools (IDT) cloned into BstXI/Blp1 (R0113S and R0585, NEB) double digested pJR104 (a gift from Jonathan Weissman) using NEBuilder HiFi DNA Assembly kit (E2627,

(19) Structural and functional properties of mSWI/SNF chromatin remodeling complexes revealed through single-cell perturbation screensMolecular cellApril 20, 2023Otto JE, Ursu O, Wu AP, Winter EB, Cuoco MS, Ma S, Qian K, Michel BC, Buenrostro JD, Berger B, et al.#BP231-100 EDTA,Fisher Scientific,Cat #BP2482 BstXI,New England Biolabs,Cat# R0113S BlpI,New England Biolabs,Cat# R0585S Critical Commercial Assays,Critical Commercial Assays,Critical Commercial Assays QIAquick Gel Extraction

(20) T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formationeLifeJanuary 31, 2023Landshammer A, Bolondi A, Kretzmer H, Much C, Buschow R, Rose A, Wu HJ, Mackowiak SD, Braendl B, Giesselmann P, et al.et al., 2014) was digested with BstXI (New England Biolabs, R0113S) and BlpI (New England Biolabs, R0585S) and the linearized plasmid was gel extracted with the QIAquick Gel Extraction Kit (Quiagen, 28704).

Be the First to Write a Review!

BlpI

BlpI from New England Biolabs

Sequence

New England Biolabs

Citations (31)

Reviews

BlpI from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map